LOCUS       Exported                3819 bp ds-DNA     circular UNA 20-MAR-2017
DEFINITION  natural circular DNA
ACCESSION   urn.local...1375724983699.8
VERSION     .
KEYWORDS    pWT057h_032017_pBR322_sgRNA-HEK4_dHHR_17_blocker_CMV-iRFP
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, May 15, 2017 from SnapGene 3.1.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     promoter        67..330
                     /label=hU6 prom
                     /note="hU6 prom promoter"
     misc_feature    331
                     /note="Geneious type: Editing History Insertion"
     misc_feature    complement(332..348)
                     /label=HEK293 site 4 blocker
                     /note="HEK293 site 4 blocker"
     misc_feature    complement(349..411)
                     /label=dHHR
                     /note="dHHR"
     primer_bind     complement(359..390)
                     /label=WT571
                     /note="WT571"
     modified_base   383
                     /label=mutation
                     /note="mutation"
     misc_feature    412..431
                     /label=HEK293 site 4
                     /note="HEK293 site 4"
     misc_feature    complement(432..514)
                     /note="gRNA backbone"
     primer_bind     complement(526..559)
                     /note="WT518"
     primer_bind     552..572
                     /note="WT517"
     misc_feature    555..2013
                     /note="Geneious type: Editing History Insertion"
     promoter        610..691
                     /note="CMV prom"
     misc_feature    781..1716
                     /note="iRFP"
     misc_feature    complement(1708..1716)
                     /note="end"
     terminator      1731..1975
                     /note="SV40_PA term"
     primer_bind     complement(1976..2010)
                     /note="WT519"
     primer_bind     2005..2035
                     /note="WT520"
     misc_feature    complement(2055..2674)
                     /note="pBR322 origin"
                     /note="Geneious type: Origin of Replication"
     misc_feature    complement(2829..3689)
                     /note="amp marker"
     promoter        complement(3731..3759)
                     /note="amp prom"
ORIGIN
        1 gacgtcgcta gctgtacaaa aaagcaggct ttaaaggaac caattcagtc gactggatcc
       61 ggtaccaagg tcgggcagga agagggccta tttcccatga ttccttcata tttgcatata
      121 cgatacaagg ctgttagaga gataattaga attaatttga ctgtaaacac aaagatatta
      181 gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta
      241 tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct
      301 ttatatatct tgtggaaagg acgaaacacc gcctccagcc gcagtgccgg tacatccagc
      361 tgatgagtcc caaataggac gagacgcgct tcggtgcgtc ctggattcca cggcactgcg
      421 gctggaggtg ggttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa
      481 cttgaaaaag tggcaccgag tcggtgcttt ttttaagctt gggccgctcg aggtacctct
      541 ctacatatga catgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      601 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      661 aagcagagct ggtttagtga accgtcagat ccgctagcgc taccggactc agatctcgag
      721 ctcaagcttc gaattctgca gtcgacggta ccgcgggccc gggatccacc ggtcgccacc
      781 atggcgcgta aggtcgatct cacctcctgc gatcgcgagc cgatccacat ccccggcagc
      841 attcagccgt gcggctgcct gctagcctgc gacgcgcagg cggtgcggat cacgcgcatt
      901 acggaaaatg ccggcgcgtt ctttggacgc gaaactccgc gggtcggtga gctactcgcc
      961 gattacttcg gcgagaccga agcccatgcg ctgcgcaacg cactggcgca gtcctccgat
     1021 ccaaagcgac cggcgctgat cttcggttgg cgcgacggcc tgaccggccg caccttcgac
     1081 atctcactgc atcgccatga cggtacatcg atcatcgagt tcgagcctgc ggcggccgaa
     1141 caggccgaca atccgctgcg gctgacgcgg cagatcatcg cgcgcaccaa agaactgaag
     1201 tcgctcgaag agatggccgc acgggtgccg cgctatctgc aggcgatgct cggctatcac
     1261 cgcgtgatgt tgtaccgctt cgcggacgac ggctccggga tggtgatcgg cgaggcgaag
     1321 cgcagcgacc tcgagagctt tctcggtcag cactttccgg cgtcgctggt cccgcagcag
     1381 gcgcggctac tgtacttgaa gaacgcgatc cgcgtggtct cggattcgcg cggcatcagc
     1441 agccggatcg tgcccgagca cgacgcctcc ggcgccgcgc tcgatctgtc gttcgcgcac
     1501 ctgcgcagca tctcgccctg ccatctcgaa tttctgcgga acatgggcgt cagcgcctcg
     1561 atgtcgctgt cgatcatcat tgacggcacg ctatggggat tgatcatctg tcatcattac
     1621 gagccgcgtg ccgtgccgat ggcgcagcgc gtcgcggccg aaatgttcgc cgacttctta
     1681 tcgctgcact tcaccgccgc ccaccaccaa cgctaagcgg ccgcgactct agatcataat
     1741 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     1801 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     1861 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     1921 ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta
     1981 atattttgtt aaaattcgcg ttaaattttt atgtgagcaa aaggccagca aaaggccagg
     2041 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     2101 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     2161 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     2221 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     2281 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     2341 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     2401 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     2461 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     2521 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     2581 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     2641 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     2701 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     2761 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     2821 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     2881 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     2941 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     3001 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     3061 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     3121 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     3181 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     3241 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     3301 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     3361 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     3421 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     3481 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     3541 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     3601 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     3661 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     3721 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     3781 ataggggttc cgcgcacatt tccccgaaaa gtgccacct
//
