LOCUS Exported 12704 bp ds-DNA circular SYN 13-APR-2017 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pXPR_121 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12704) AUTHORS . TITLE Direct Submission JOURNAL Exported Wednesday, May 17, 2017 from SnapGene 3.1.3 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12704 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 18..1276 /note="EF1a" promoter 98..1276 /note="EF-1-alpha promoter" /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 329..1267 /note="EF-1-alpha intron A" /note="intron upstream of the start codon of human EF-1-alpha" protein_bind 1296..1320 /gene="mutant version of attB" /bound_moiety="BP Clonase(TM)" /note="attB1" /note="recombination site for the Gateway(R) BP reaction" CDS 1320..1535 /codon_start=1 /note="KRAB" /translation="MDAKSLTAWSRTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLE NYKNLVSLGYQLTKPDVILRLEKGEEP" CDS 1350..1535 /codon_start=1 /gene="ZNF10" /gene_synonym="KOX1" /product="Kruppel-associated box (KRAB) transcriptional repression domain from the human zinc finger protein ZNF10 (Margolin et al., 1994)" /note="KRAB" /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY QLTKPDVILRLEKGEEP" CDS 1536..1556 /codon_start=1 /note="linker" /translation="GGSGGGS" CDS 1557..5660 /codon_start=1 /note="dCas9" /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" CDS 5664..5702 /codon_start=1 /note="HA" /translation="YPYDVPDYASLGS" CDS 5664..5690 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /note="HA" /translation="YPYDVPDYA" CDS 5703..5798 /codon_start=1 /note="NLS" /translation="GSPKKKRKVEDPKKKRKVDGIGSGSNGSSGS" CDS 5709..5729 /codon_start=1 /product="nuclear localization signal of SV40 large T antigen" /note="SV40 NLS" /translation="PKKKRKV" CDS 5736..5756 /codon_start=1 /product="nuclear localization signal of SV40 large T antigen" /note="SV40 NLS" /translation="PKKKRKV" protein_bind complement(5801..5825) /gene="mutant version of attB" /bound_moiety="BP Clonase(TM)" /note="attB2" /note="recombination site for the Gateway(R) BP reaction" CDS 5833..5874 /codon_start=1 /product="epitope tag from simian virus 5" /note="V5 tag" /translation="GKPIPNPLLGLDST" misc_binding 5925..6513 /note="WPRE" misc_feature 5925..6513 /note="WPRE" /note="woodchuck hepatitis virus posttranscriptional regulatory element" CDS complement(6396..6407) /codon_start=1 /product="Factor Xa recognition and cleavage site" /note="Factor Xa site" /translation="IEGR" 3'UTR 6584..6819 /note="3'LTR" LTR 6585..6818 /note="3' LTR (Delta-U3)" /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 6896..7026 /note="SV40 pA" /note="SV40 polyadenylation signal" polyA_signal 6896..7017 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" rep_origin 7057..7192 /note="SV40 ori" /note="SV40 origin of replication" promoter complement(7213..7231) /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(7241..7257) /note="M13 fwd" /note="common sequencing primer, one of multiple similar variants" rep_origin 7399..7854 /note="F1 ori" promoter 7880..7984 /gene="bla" /note="AmpR promoter" gene 7985..8845 /note="AmpR" CDS 7985..8845 /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 9016..9604 /direction=RIGHT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" rep_origin 9106..9721 /note="pUC Ori" protein_bind 9892..9913 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding site" /note="CAP binding activates transcription in the presence of cAMP." promoter 9928..9958 /note="lac promoter" /note="promoter for the E. coli lac operon" protein_bind 9966..9982 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 9990..10006 /note="M13 rev" /note="common sequencing primer, one of multiple similar variants" promoter 10027..10045 /note="T3 promoter" /note="promoter for bacteriophage T3 RNA polymerase" LTR 10069..10481 /label=5'LTR /note="5'LTR" promoter 10073..10299 /note="RSV promoter" /note="Rous sarcoma virus enhancer/promoter" LTR 10300..10480 /note="5' LTR (truncated)" /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 10527..10652 /note="HIV-1 Psi" /note="packaging signal of human immunodeficiency virus type 1" misc_feature 10532..10669 /label=psi /note="psi" misc_feature 10636..11000 /label=gag /note="gag" misc_feature 11145..11378 /note="RRE" /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." misc_feature 11146..11387 /label=RRE /note="RRE" misc_feature 11788..12062 /note="SV40" promoter 11838..12048 /note="SV40 promoter" /note="SV40 early promoter" promoter 11860..12056 /note="SV40 promoter" /note="SV40 early promoter" rep_origin 11907..12042 /note="SV40 ori" /note="SV40 origin of replication" CDS 12078..12473 /codon_start=1 /note="BlastR" /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI KAIVKDSDGQPTAVGIRELLPSGYVWEG" misc_feature 12547..12664 /label=cPPT /note="cPPT" ORIGIN 1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag 61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc 121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg 181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct 241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt 301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg 361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt 421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta 481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt 541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta 601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg 661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc 721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg 781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc 841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc 901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc 961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga 1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac 1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt 1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt 1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc 1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca 1321 tggatgctaa gtcactaact gcctggtccc ggacactggt gaccttcaag gatgtatttg 1381 tggacttcac cagggaggag tggaagctgc tggacactgc tcagcagatc gtgtacagaa 1441 atgtgatgct ggagaactat aagaacctgg tttccttggg ttatcagctt actaagccag 1501 atgtgatcct ccggttggag aagggagaag agcccggcgg ttccggcgga gggtcgatgg 1561 acaagaagta cagcatcggc ctggccatcg gcaccaactc tgtgggctgg gccgtgatca 1621 ccgacgagta caaggtgccc agcaagaaat tcaaggtgct gggcaacacc gaccggcaca 1681 gcatcaagaa gaacctgatc ggcgccctgc tgttcgacag cggagaaaca gccgaggcca 1741 cccggctgaa gagaaccgcc agaagaagat acaccagacg gaagaaccgg atctgctatc 1801 tgcaagagat cttcagcaac gagatggcca aggtggacga cagcttcttc cacagactgg 1861 aagagtcctt cctggtggaa gaggataaga agcacgagcg gcaccccatc ttcggcaaca 1921 tcgtggacga ggtggcctac cacgagaagt accccaccat ctaccacctg agaaagaaac 1981 tggtggacag caccgacaag gccgacctgc ggctgatcta tctggccctg gcccacatga 2041 tcaagttccg gggccacttc ctgatcgagg gcgacctgaa ccccgacaac agcgacgtgg 2101 acaagctgtt catccagctg gtgcagacct acaaccagct gttcgaggaa aaccccatca 2161 acgccagcgg cgtggacgcc aaggccatcc tgtctgccag actgagcaag agcagacggc 2221 tggaaaatct gatcgcccag ctgcccggcg agaagaagaa tggcctgttc ggcaacctga 2281 ttgccctgag cctgggcctg acccccaact tcaagagcaa cttcgacctg gccgaggatg 2341 ccaaactgca gctgagcaag gacacctacg acgacgacct ggacaacctg ctggcccaga 2401 tcggcgacca gtacgccgac ctgtttctgg ccgccaagaa cctgtccgac gccatcctgc 2461 tgagcgacat cctgagagtg aacaccgaga tcaccaaggc ccccctgagc gcctctatga 2521 tcaagagata cgacgagcac caccaggacc tgaccctgct gaaagctctc gtgcggcagc 2581 agctgcctga gaagtacaaa gagattttct tcgaccagag caagaacggc tacgccggct 2641 acatcgatgg cggagccagc caggaagagt tctacaagtt catcaagccc atcctggaaa 2701 agatggacgg caccgaggaa ctgctcgtga agctgaacag agaggacctg ctgcggaagc 2761 agcggacctt cgacaacggc agcatccccc accagatcca cctgggagag ctgcacgcca 2821 ttctgcggcg gcaggaagat ttttacccat tcctgaagga caaccgggaa aagatcgaga 2881 agatcctgac cttccgcatc ccctactacg tgggccctct ggccagggga aacagcagat 2941 tcgcctggat gaccagaaag agcgaggaaa ccatcacccc ctggaacttc gaggaagtgg 3001 tggacaaggg cgccagcgcc cagagcttca tcgagcggat gaccaacttc gataagaacc 3061 tgcccaacga gaaggtgctg cccaagcaca gcctgctgta cgagtacttc accgtgtaca 3121 acgagctgac caaagtgaaa tacgtgaccg agggaatgag aaagcccgcc ttcctgagcg 3181 gcgagcagaa aaaagccatc gtggacctgc tgttcaagac caaccggaaa gtgaccgtga 3241 agcagctgaa agaggactac ttcaagaaaa tcgagtgctt cgactccgtg gaaatctccg 3301 gcgtggaaga tcggttcaac gcctccctgg gcacatacca cgatctgctg aaaattatca 3361 aggacaagga cttcctggac aatgaggaaa acgaggacat tctggaagat atcgtgctga 3421 ccctgacact gtttgaggac agagagatga tcgaggaacg gctgaaaacc tatgcccacc 3481 tgttcgacga caaagtgatg aagcagctga agcggcggag atacaccggc tggggcaggc 3541 tgagccggaa gctgatcaac ggcatccggg acaagcagtc cggcaagaca atcctggatt 3601 tcctgaagtc cgacggcttc gccaacagaa acttcatgca gctgatccac gacgacagcc 3661 tgacctttaa agaggacatc cagaaagccc aggtgtccgg ccagggcgat agcctgcacg 3721 agcacattgc caatctggcc ggcagccccg ccattaagaa gggcatcctg cagacagtga 3781 aggtggtgga cgagctcgtg aaagtgatgg gccggcacaa gcccgagaac atcgtgatcg 3841 aaatggccag agagaaccag accacccaga agggacagaa gaacagccgc gagagaatga 3901 agcggatcga agagggcatc aaagagctgg gcagccagat cctgaaagaa caccccgtgg 3961 aaaacaccca gctgcagaac gagaagctgt acctgtacta cctgcagaat gggcgggata 4021 tgtacgtgga ccaggaactg gacatcaacc ggctgtccga ctacgatgtg gacgctatcg 4081 tgcctcagag ctttctgaag gacgactcca tcgataacaa agtgctgact cggagcgaca 4141 agaaccgggg caagagcgac aacgtgccct ccgaagaggt cgtgaagaag atgaagaact 4201 actggcgcca gctgctgaat gccaagctga ttacccagag gaagttcgac aatctgacca 4261 aggccgagag aggcggcctg agcgaactgg ataaggccgg cttcatcaag agacagctgg 4321 tggaaacccg gcagatcaca aagcacgtgg cacagatcct ggactcccgg atgaacacta 4381 agtacgacga gaacgacaaa ctgatccggg aagtgaaagt gatcaccctg aagtccaagc 4441 tggtgtccga tttccggaag gatttccagt tttacaaagt gcgcgagatc aacaactacc 4501 accacgccca cgacgcctac ctgaacgccg tcgtgggaac cgccctgatc aaaaagtacc 4561 ctaagctgga aagcgagttc gtgtacggcg actacaaggt gtacgacgtg cggaagatga 4621 tcgccaagag cgagcaggaa atcggcaagg ctaccgccaa gtacttcttc tacagcaaca 4681 tcatgaactt tttcaagacc gagattaccc tggccaacgg cgagatccgg aagcggcctc 4741 tgatcgagac aaacggcgaa acaggcgaga tcgtgtggga taagggccgg gactttgcca 4801 ccgtgcggaa agtgctgtct atgccccaag tgaatatcgt gaaaaagacc gaggtgcaga 4861 caggcggctt cagcaaagag tctatcctgc ccaagaggaa cagcgacaag ctgatcgcca 4921 gaaagaagga ctgggaccct aagaagtacg gcggcttcga cagccccacc gtggcctatt 4981 ctgtgctggt ggtggccaaa gtggaaaagg gcaagtccaa gaaactgaag agtgtgaaag 5041 agctgctggg gatcaccatc atggaaagaa gcagcttcga gaagaatccc atcgactttc 5101 tggaagccaa gggctacaaa gaagtgaaaa aggacctgat catcaagctg cctaagtact 5161 ccctgttcga gctggaaaac ggccggaaga gaatgctggc ctctgccggc gaactgcaga 5221 agggaaacga actggccctg ccctccaaat atgtgaactt cctgtacctg gccagccact 5281 atgagaagct gaagggctcc cccgaggata atgagcagaa acagctgttt gtggaacagc 5341 acaaacacta cctggacgag atcatcgagc agatcagcga gttctccaag agagtgatcc 5401 tggccgacgc taatctggac aaggtgctga gcgcctacaa caagcacaga gacaagccta 5461 tcagagagca ggccgagaat atcatccacc tgtttaccct gaccaatctg ggagcccctg 5521 ccgccttcaa gtactttgac accaccatcg accggaagag gtacaccagc accaaagagg 5581 tgctggacgc caccctgatc caccagagca tcaccggcct gtacgagaca cggatcgacc 5641 tgtctcagct gggaggcgac gcctatccct atgacgtgcc cgattatgcc agcctgggca 5701 gcggctcccc caagaaaaaa cgcaaggtgg aagatcctaa gaaaaagcgg aaagtggacg 5761 gcattggtag tgggagcaac ggcagcagcg gatcctaaac ccccaacttt cttgtacaaa 5821 gtggttgata tcggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa 5881 tgaactagta ccggttaagt cgacaatcaa cgcgttaagt cgacaatcaa cctctggatt 5941 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg 6001 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct 6061 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc 6121 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca 6181 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac 6241 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt 6301 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct 6361 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc 6421 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga 6481 cgagtcggat ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa 6541 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca 6601 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat 6661 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt 6721 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc 6781 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt acgtatagta gttcatgtca 6841 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt 6901 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 6961 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca 7021 tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt 7081 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc 7141 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg 7201 tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac 7261 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt 7321 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca 7381 gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg 7441 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct 7501 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc 7561 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg 7621 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt 7681 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg 7741 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc 7801 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg 7861 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa 7921 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa 7981 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct 8041 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg 8101 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg 8161 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt 8221 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga 8281 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga 8341 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac 8401 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg 8461 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac 8521 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct 8581 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct 8641 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg 8701 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat 8761 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg 8821 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat 8881 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct 8941 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa 9001 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa 9061 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc 9121 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta 9181 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct 9241 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg 9301 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag 9361 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc 9421 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg 9481 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt 9541 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg 9601 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca 9661 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg 9721 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc 9781 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag 9841 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag 9901 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg 9961 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa 10021 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct 10081 tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa 10141 ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta 10201 ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc 10261 agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag 10321 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc 10381 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga 10441 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact 10501 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc 10561 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag 10621 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc 10681 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag 10741 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag 10801 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac 10861 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa 10921 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg 10981 cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga 11041 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag 11101 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt 11161 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag 11221 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag 11281 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc 11341 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga 11401 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa 11461 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc 11521 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta 11581 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg 11641 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct 11701 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac 11761 ctcccaaccc cgaggggacc catgcattgc atctcaatta gtcagcaacc aggtgtggaa 11821 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcgtctcaat tagtcagcaa 11881 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt 11941 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 12001 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc 12061 tttctagagg taccaccatg gccaagcctt tgtctcaaga agaatccacc ctcattgaaa 12121 gagcaacggc tacaatcaac agcatcccca tctctgaaga ctacagcgtc gccagcgcag 12181 ctctctctag cgacggccgc atcttcactg gtgtcaatgt atatcatttt actgggggac 12241 cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc ggcagctggc aacctgactt 12301 gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag cccctgcgga cggtgccgac 12361 aggtgcttct cgatctgcat cctgggatca aagccatagt gaaggacagt gatggacagc 12421 cgacggcagt tgggattcgt gaattgctgc cctctggtta tgtgtgggag ggcctgcagc 12481 tgcagtagta aggcgcgccg ttaacgaatt ctagatcttg agacaaatgg cagtattcat 12541 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga 12601 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa 12661 ttttcgggtt tattacaggg acagcagaga tccactttgg cgcc //