LOCUS       Exported                8694 bp ds-DNA     circular SYN 29-JAN-2010
DEFINITION  -.
ACCESSION   -
VERSION     .
KEYWORDS    pXPR_502
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8694)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, May 17, 2017 from SnapGene 3.1.3
            http://www.snapgene.com
COMMENT     formerly pXPR_024-Tr14-P104
FEATURES             Location/Qualifiers
     source          1..8694
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..413
                     /label=5'LTR
                     /note="5'LTR"
     misc_feature    1..231
                     /note="RSV"
     misc_feature    464..601
                     /label=psi
                     /note="psi"
     misc_feature    568..932
                     /label=gag
                     /note="gag"
     misc_feature    1078..1319
                     /label=RRE
                     /note="RRE"
     promoter        1717..1966
                     /note="hU6"
     misc_RNA        1992..2197
                     /note="2xPP7-2xMS2"
     misc_feature    2239..2356
                     /note="CPPT"
     promoter        2415..2917
                     /label=PGK
                     /note="PGK promoter"
     CDS             2941..3483
                     /note="PP7-NLS"
     CDS             3490..4428
                     /note="P65-HSF"
     misc_feature    4435..4497
                     /note="2A"
     CDS             4525..5130
                     /note="PuroR"
     LTR             5210..5445
                     /label=3'\LTR\SIN
                     /note="3' LTR SIN"
     polyA_signal    5522..5652
                     /note="SV40 polyadenylation signal"
     rep_origin      5732..5807
                     /note="SV40 origin of replication"
     rep_origin      6025..6480
                     /note="F1 ori"
     CDS             6611..7471
                     /note="AmpR"
     rep_origin      7619..8286
                     /note="pUC ori"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccagagagg gcctatttcc catgattcct
     1741 tcatatttgc atatacgata caaggctgtt agagagataa ttagaattaa tttgactgta
     1801 aacacaaaga tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt
     1861 gcagttttaa aattatgttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat
     1921 ttcgatttct tggctttata tatcttgtgg aaaggacgaa acaccggaga cgaccggttt
     1981 tttgcgtctc tgtttaagag ctatgctgcg aatacgaggg agcagacgat atggcgtcgc
     2041 tcctctccac gagagcatat gggctccgtg gtctcgtaca ccatcagggt acgtatcaga
     2101 caccatcagg gtctgctcgt attcgcagca tagcaagttt aaataaggct agtccgttat
     2161 caacttgaaa aagtggcacc gagtcggtgc tttttttgaa ttctagatct tgagacaaat
     2221 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
     2281 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
     2341 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccacttt ggcgccggct
     2401 cgagggggcc cgggttgcgc cttttccaag gcagccctgg gtttgcgcag ggacgcggct
     2461 gctctgggcg tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc acattcttca
     2521 cgtccgttcg cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc
     2581 ttcctgctcc gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac
     2641 aaacggaagc cgcacgtctt actagtaccc tcgcagacgg acagcgccag ggagcaatgg
     2701 cagcgcgccg accgcgatgg gctgtggcca atagcggctg ctcagcaggg cgcgccgaga
     2761 gcagcggccg ggaaggggcg gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt
     2821 tcctgcccgc gcggtgttcc gcattctgca agcctccgga gcgcacgtcg gcagtcggct
     2881 ccctcgttga ccgaatcacc gacctctctc cccaggggga tccaacttaa gcttgccacc
     2941 atgtcgcgga ggcggcacag ctacgagaac gatggtggac aacctcacaa aaggaggaag
     3001 acgtctatgc tagcctccaa aaccatcgtt ctttcggtcg gcgaggctac tcgcactctg
     3061 actgagatcc agtccaccgc agaccgtcag atcttcgaag agaaggtcgg gcctctggtg
     3121 ggtcggctgc gcctcacggc ttcgctccgt caaaacggag ccaagaccgc gtatcgagtc
     3181 aacctaaaac tggatcaggc ggacgtcgtt gattccggac ttccgaaagt gcgctacact
     3241 caggtatggt cgcacgacgt gacaatcgtt gcgaatagca ccgaggcctc gcgcaaatcg
     3301 ttgtacgatt tgaccaagtc cctcgtcgcg acctcgcagg tcgaagatct tgtcgtcaac
     3361 cttgtgccgc tgggccgttc ttcaactttc ttgtacaaag tgggcggagg aggtggaagc
     3421 ggaggaggag gaagcggagg aggaggtagc ggacctaaga aaaagaggaa ggtggcggcc
     3481 gctggtaccc cttcagggca gatcagcaac caggccctgg ctctggcccc tagctccgct
     3541 ccagtgctgg cccagactat ggtgccctct agtgctatgg tgcctctggc ccagccacct
     3601 gctccagccc ctgtgctgac cccaggacca ccccagtcac tgagcgctcc agtgcccaag
     3661 tctacacagg ccggcgaggg gactctgagt gaagctctgc tgcacctgca gttcgacgct
     3721 gatgaggacc tgggagctct gctggggaac agcaccgatc ccggagtgtt cacagatctg
     3781 gcctccgtgg acaactctga gtttcagcag ctgctgaatc agggcgtgtc catgtctcat
     3841 agtacagccg aaccaatgct gatggagtac cccgaagcca ttacccggct ggtgaccggc
     3901 agccagcggc cccccgaccc cgctccaact cccctgggaa ccagcggcct gcctaatggg
     3961 ctgtccggag atgaagactt ctcaagcatc gctgatatgg actttagtgc cctgctgtca
     4021 cagatttcct ctagtgggca gggaggaggt ggaagcggct tcagcgtgga caccagtgcc
     4081 ctgctggacc tgttcagccc ctcggtgacc gtgcccgaca tgagcctgcc tgaccttgac
     4141 agcagcctgg ccagtatcca agagctcctg tctccccagg agccccccag gcctcccgag
     4201 gcagagaaca gcagcccgga ttcagggaag cagctggtgc actacacagc gcagccgctg
     4261 ttcctgctgg accccggctc cgtggacacc gggagcaacg acctgccggt gctgtttgag
     4321 ctgggagagg gctcctactt ctccgaaggg gacggcttcg ccgaggaccc caccatctcc
     4381 ctgctgacag gctcggagcc tcccaaagcc aaggacccca ctgtctccga tatcggcagt
     4441 ggagagggca gaggaagtct gctaacatgc ggtgacgtcg aggagaatcc tggcccaggt
     4501 accacagatc caccggagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc
     4561 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     4621 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     4681 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcc
     4741 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     4801 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     4861 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtttcgccc
     4921 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     4981 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     5041 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     5101 atgacccgca agcccggtgc cgctagctga acgcgttaag tcgactttaa gaccaatgac
     5161 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     5221 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     5281 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     5341 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     5401 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtacgt atagtagttc
     5461 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     5521 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     5581 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     5641 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     5701 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     5761 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     5821 gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     5881 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     5941 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     6001 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     6061 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     6121 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     6181 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     6241 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     6301 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     6361 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     6421 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     6481 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     6541 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     6601 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     6661 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     6721 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     6781 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     6841 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     6901 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     6961 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     7021 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     7081 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     7141 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     7201 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     7261 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     7321 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     7381 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     7441 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     7501 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     7561 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     7621 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     7681 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     7741 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     7801 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     7861 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     7921 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     7981 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     8041 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     8101 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     8161 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     8221 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     8281 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     8341 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     8401 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     8461 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     8521 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     8581 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     8641 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagc
//
