LOCUS       Exported                8343 bp ds-DNA     circular SYN 21-MAR-2017
DEFINITION  GPP_Production_Plasmids_1.E06.
ACCESSION   .
VERSION     .
KEYWORDS    pXPR_050
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8343)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, May 17, 2017 from SnapGene 3.1.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8343
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    186..774
                     /note="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     LTR             846..1079
                     /note="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1157..1278
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      1318..1453
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     rep_origin      1660..2115
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        2141..2245
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             2246..3106
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3277..3865
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        4334..4560
                     /note="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4561..4741
                     /note="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4793..4930
                     /note="psi"
                     /note="psi"
     misc_feature    4897..5261
                     /note="gag"
                     /note="gag"
     misc_feature    5406..5639
                     /note="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     promoter        6046..6286
                     /note="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_feature    6468..6585
                     /note="cPPT/CTS"
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6723..7901
                     /note="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     CDS             7914..170
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /note="PuroR"
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
ORIGIN
        1 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg
       61 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
      121 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga acgcgttaag
      181 tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
      241 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
      301 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
      361 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
      421 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
      481 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
      541 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
      601 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
      661 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
      721 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac
      781 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
      841 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
      901 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
      961 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     1021 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     1081 tacgtatagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     1141 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     1201 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     1261 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc
     1321 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     1381 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     1441 ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct
     1501 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     1561 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     1621 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat
     1681 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     1741 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     1801 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     1861 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     1921 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     1981 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     2041 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     2101 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     2161 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     2221 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     2281 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     2341 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     2401 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     2461 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     2521 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     2581 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     2641 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     2701 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     2761 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     2821 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     2881 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     2941 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     3001 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     3061 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     3121 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     3181 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     3241 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     3301 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     3361 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     3421 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     3481 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     3541 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     3601 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     3661 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     3721 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     3781 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     3841 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     3901 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     3961 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     4021 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     4081 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     4141 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     4201 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     4261 ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga
     4321 gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg
     4381 agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta
     4441 aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga
     4501 accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac
     4561 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     4621 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     4681 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     4741 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
     4801 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
     4861 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
     4921 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
     4981 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
     5041 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     5101 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     5161 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     5221 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
     5281 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     5341 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     5401 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     5461 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     5521 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     5581 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     5641 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     5701 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     5761 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     5821 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     5881 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     5941 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     6001 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccagagaggg cctatttccc
     6061 atgattcctt catatttgca tatacgatac aaggctgtta gagagataat tagaattaat
     6121 ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt
     6181 gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact
     6241 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccggagac
     6301 gaccggtttt ttgcgtctct gtttcagagc tacagcagaa atgctgtagc aagttgaaat
     6361 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttgaattcaa
     6421 gcttggcgta actagatctt gagacaaatg gcagtattca tccacaattt taaaagaaaa
     6481 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     6541 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
     6601 gacagcagag atccactttg gcgccggctc agggggcccg ggtgcaaaga tggataaagt
     6661 tttaaacaga gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa
     6721 ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     6781 ggggaggggt cggcaattga tccggtgcct agagaaggtg gcgcggggta aactgggaaa
     6841 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     6901 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     6961 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     7021 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     7081 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     7141 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     7201 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     7261 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     7321 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     7381 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     7441 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     7501 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     7561 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     7621 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     7681 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     7741 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     7801 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     7861 cagtggttca aagttttttt cttccatttc aggtgtcgtg acgtacggcc accatgaccg
     7921 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc
     7981 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca
     8041 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
     8101 aggtgtgggt cgcggacgac ggcgccgccg tggcggtctg gaccacgccg gagagcgtcg
     8161 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
     8221 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
     8281 ggttcctggc caccgtcgga gtctcgcccg accaccaggg caagggtctg ggcagcgccg
     8341 tcg
//
