LOCUS pMGF170_pGEM_GST 7529 bp ds-DNA circular 27-JUL-2015 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers misc_binding 5..27 /label=LacO /ApEinfo_fwdcolor=cornflower blue /ApEinfo_revcolor=cornflower blue /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2663..3235 /label=NAT ORF /ApEinfo_fwdcolor=#006410 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1055..1295 /label=ish1-tail /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3203..3222 /label=NAT internal reverse primer /ApEinfo_fwdcolor=#00ffff /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 708..734 /label=PreScissionProtease /ApEinfo_fwdcolor=#ecec00 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2320..2332 /label=Begin MX Cassette 4/6 FOR /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1326..2033 /label=mCherry /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3449..3467 /label=End MX Cassette Rev primer 4/6 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 4618..5277 /label=AmpR /ApEinfo_fwdcolor=yellow /ApEinfo_revcolor=yellow /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 48..707 /label=GST /ApEinfo_fwdcolor=#66ffff /ApEinfo_revcolor=#66ffff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 rep_origin 5375..6057 /label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 645..664 /label=pGEX-GST-FOR-primer /ApEinfo_fwdcolor=#ff8000 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1326..2033 /label=mCherry(1) /ApEinfo_label=mCherry /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 735..754 /label=oMGF-486 /ApEinfo_fwdcolor=#c0c0c0 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3975..3996 /label=oMGF-485 /ApEinfo_fwdcolor=#007575 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1296..1325 /label=3'linker /ApEinfo_fwdcolor=#8000ff /ApEinfo_revcolor=#8000ff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2034..2035 /label=3X HA /ApEinfo_fwdcolor=#ff8000 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2057..2236 /label=yADH1_terminator /ApEinfo_fwdcolor=#804040 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2319..2319 /label=MX6-seq /ApEinfo_fwdcolor=#c0c0c0 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2573..2592 /label=MX cassette check reverse primer /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ORIGIN 1 nnnnGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC AGTATTCATG TCCCCTATAC 61 TAGGTTATTG GAAAATTAAG GGCCTTGTGC AACCCACTCG ACTTCTTTTG GAATATCTTG 121 AAGAAAAATA TGAAGAGCAT TTGTATGAGC GCGATGAAGG TGATAAATGG CGAAACAAAA 181 AGTTTGAATT GGGTTTGGAG TTTCCCAATC TTCCTTATTA TATTGATGGT GATGTTAAAT 241 TAACACAGTC TATGGCCATC ATACGTTATA TAGCTGACAA GCACAACATG TTGGGTGGTT 301 GTCCAAAAGA GCGTGCAGAG ATTTCAATGC TTGAAGGAGC GGTTTTGGAT ATTAGATACG 361 GTGTTTCGAG AATTGCATAT AGTAAAGACT TTGAAACTCT CAAAGTTGAT TTTCTTAGCA 421 AGCTACCTGA AATGCTGAAA ATGTTCGAAG ATCGTTTATG TCATAAAACA TATTTAAATG 481 GTGATCATGT AACCCATCCT GACTTCATGT TGTATGACGC TCTTGATGTT GTTTTATACA 541 TGGACCCAAT GTGCCTGGAT GCGTTCCCAA AATTAGTTTG TTTTAAAAAA CGTATTGAAG 601 CTATCCCACA AATTGATAAG TACTTGAAAT CCAGCAAGTA TATAGCATGG CCTTTGCAGG 661 GCTGGCAAGC CACGTTTGGT GGTGGCGACC ATCCTCCAAA ATCGGATctg gaagttctgt 721 tccaggggcc cctgGCTGTT CCTAATTGGG CTGCTTATTT TGATACTTTG AGCTCCAAAG 781 AGCTTTCTGC CTGGATTAAG GAATACAAAA AACATTACCA TGGAAAACTT CACCCATCCA 841 AAGATCGTGA ACATCTTTTC CAAAATGCCT GTAATATTTA TCGCGCTATT GCTGAAAAGG 901 CTGACAAATC TGTTCTTCAA TCGATTCAAT CAAAGTTTCC TAAGGCAACT GTTCCCGGTT 961 ATGACTCTTG GTCTAATGAA GATCTAAAGT CTGCACTCAA GGAATATGGT GACAGTATTG 1021 GTAAGGTGTT TAATCGTAAA GATGCTATTG AACGCTTAAA GCGTCATGAT ATTTTATTCT 1081 ATGGTCCTGT TGTTGCCGAC AAGGCTAAAT CAGGAGTCTC TGGCTTCCTT CGACGTGTTG 1141 CTTCTtttat gggatttggc actcgcgacg tagctgaaat taagctagga gaaaacattc 1201 aaaaggtaaa agatactgcc tctaaagcct catctgctgt atcttcagca ggcgactatg 1261 tcgaaactaa tgtaaagaaa gcccaaaggg ttttgggagg aacgggcggt agtggaggca 1321 ctggtatggt gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct 1381 tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg 1441 agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc 1501 tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga 1561 agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg 1621 agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc 1681 aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc 1741 ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg 1801 acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg 1861 acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca 1921 acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt 1981 acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac aagTGAGGCG 2041 CGCCACTTCT AAATAAGCGA ATTTCTTATG ATTTATGATT TTTATTATTA AATAAGTTAT 2101 AAAAAAAATA AGTGTATACA AATTTTAAAG TGACTCTTAG GTTTTAAAAC GAAAATTCTT 2161 ATTCTTGAGT AACTCTTTCC TGTAGGTCAG GTTGCTTTCT CAGGTATAGT ATGAGGTCGC 2221 TCTTATTGAC CACACCTCTA CCGGCAGATC CGCTAGGGAT AACAGGGTAA TATAGATCTG 2281 TTTAGCTTGC CTCGTCCCCG CCGGGTCACC CGGCCAGCGA CATGGAGGCC CAGAATACCC 2341 TCCTTGACAG TCTTGACGTG CGCAGCTCAG GGGCATGATG TGACTGTCGC CCGTACATTT 2401 AGCCCATACA TCCCCATGTA TAATCATTTG CATCCATACA TTTTGATGGC CGCACGGCGC 2461 GAAGCAAAAA TTACGGCTCC TCGCTGCAGA CCTGCGAGCA GGGAAACGCT CCCCTCACAG 2521 ACGCGTTGAA TTGTCCCCAC GCCGCGCCCC TGTAGAGAAA TATAAAAGGT TAGGATTTGC 2581 CACTGAGGTT CTTCTTTCAT ATACTTCCTT TTAAAATCTT GCTAGGATAC AGTTCTCACA 2641 TCACATCCGA ACATAAACAA CCATGGGTAC CACTCTTGAC GACACGGCTT ACCGGTACCG 2701 CACCAGTGTC CCGGGGGACG CCGAGGCCAT CGAGGCACTG GATGGGTCCT TCACCACCGA 2761 CACCGTCTTC CGCGTCACCG CCACCGGGGA CGGCTTCACC CTGCGGGAGG TGCCGGTGGA 2821 CCCGCCCCTG ACCAAGGTGT TCCCCGACGA CGAATCGGAC GACGAATCGG ACGACGGGGA 2881 GGACGGCGAC CCGGACTCCC GGACGTTCGT CGCGTACGGG GACGACGGCG ACCTGGCGGG 2941 CTTCGTGGTC GTCTCGTACT CCGGCTGGAA CCGCCGGCTG ACCGTCGAGG ACATCGAGGT 3001 CGCCCCGGAG CACCGGGGGC ACGGGGTCGG GCGCGCGTTG ATGGGGCTCG CGACGGAGTT 3061 CGCCCGCGAG CGGGGCGCCG GGCACCTCTG GCTGGAGGTC ACCAACGTCA ACGCACCGGC 3121 GATCCACGCG TACCGGCGGA TGGGGTTCAC CCTCTGCGGC CTGGACACCG CCCTGTACGA 3181 CGGCACCGCC TCGGACGGCG AGCAGGCGCT CTACATGAGC ATGCCCTGCC CCTAATCAGT 3241 ACTGACAATA AAAAGATTCT TGTTTTCAAG AACTTGTCAT TTGTATAGTT TTTTTATATT 3301 GTAGTTGTTC TATTTTAATC AAATGTTAGC GTGATTTATA TTTTTTTTCG CCTCGACATC 3361 ATCTGCCCAG ATGCGAAGTT AAGTGCGCAG AAAGTAATAT CATGCGTCAA TCGTATGTGA 3421 ATGCTGGTCG CTATACTGCT GTCGATTCGA TACTAACGCC GCCATCCAta aattgttcat 3481 gaacgttctt aatgtctatt tatattattt aaaaagaagg agattacgtc taaaacgagt 3541 tctcgtttaa tatatgactt ttaatgaaat atctatgttc atattcgcta cttaaccttc 3601 cttggcaagt aatccatcct attgttaaca ttaatataat ccatcaagat acttttttat 3661 taatttaagt ttttcagaat tagatgttgg tgcacctcct ccaaatctag agctgtggaa 3721 agaattggta aaatcttatc gcgatgttcg tgattctcta aaaattgcag taaaacattt 3781 ctagcatatt ctttgttaat caattcttta gaaatattgc ttataacagg tggctcgaat 3841 gaacctgctg aatcacttga agctttgggc aatatttcac tgctcataac actggacagc 3901 tcgctctcgt gagatttgac actttccttt tgaacctttt gcttttcaat gagttctttt 3961 atttcatccc ttgactccac taactcgttt ccaacactcg agcggccgca tcgtgactga 4021 ctgacgatct gcctcgcgcg ttTCGGTGAT GACGGTGAAA ACCTCTGACA CATGCAGCTC 4081 CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC CCGTCAGGGC 4141 GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC GCAGCCATGA CCCAGTCACG TAGCGATAGC 4201 GGAGTGTATA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT 4261 GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA 4321 ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA 4381 CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT 4441 GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG 4501 CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG 4561 GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG 4621 AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG 4681 CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA 4741 GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG 4801 AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC 4861 GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG 4921 AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG 4981 TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC 5041 TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG 5101 TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG 5161 GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT 5221 ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA 5281 CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT 5341 AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG 5401 TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT 5461 TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT 5521 TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG 5581 CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT 5641 GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC 5701 GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG 5761 TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA 5821 CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG 5881 GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG 5941 GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA 6001 TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT 6061 TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT 6121 GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA 6181 ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT 6241 CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATAAATT CCGACACCAT CGAATGGTGC 6301 AAAACCTTTC GCGGTATGGC ATGATAGCGC CCGGAAGAGA GTCAATTCAG GGTGGTGAAT 6361 GTGAAACCAG TAACGTTATA CGATGTCGCA GAGTATGCCG GTGTCTCTTA TCAGACCGTT 6421 TCCCGCGTGG TGAACCAGGC CAGCCACGTT TCTGCGAAAA CGCGGGAAAA AGTGGAAGCG 6481 GCGATGGCGG AGCTGAATTA CATTCCCAAC CGCGTGGCAC AACAACTGGC GGGCAAACAG 6541 TCGTTGCTGA TTGGCGTTGC CACCTCCAGT CTGGCCCTGC ACGCGCCGTC GCAAATTGTC 6601 GCGGCGATTA AATCTCGCGC CGATCAACTG GGTGCCAGCG TGGTGGTGTC GATGGTAGAA 6661 CGAAGCGGCG TCGAAGCCTG TAAAGCGGCG GTGCACAATC TTCTCGCGCA ACGCGTCAGT 6721 GGGCTGATCA TTAACTATCC GCTGGATGAC CAGGATGCCA TTGCTGTGGA AGCTGCCTGC 6781 ACTAATGTTC CGGCGTTATT TCTTGATGTC TCTGACCAGA CACCCATCAA CAGTATTATT 6841 TTCTCCCATG AAGACGGTAC GCGACTGGGC GTGGAGCATC TGGTCGCATT GGGTCACCAG 6901 CAAATCGCGC TGTTAGCGGG CCCATTAAGT TCTGTCTCGG CGCGTCTGCG TCTGGCTGGC 6961 TGGCATAAAT ATCTCACTCG CAATCAAATT CAGCCGATAG CGGAACGGGA AGGCGACTGG 7021 AGTGCCATGT CCGGTTTTCA ACAAACCATG CAAATGCTGA ATGAGGGCAT CGTTCCCACT 7081 GCGATGCTGG TTGCCAACGA TCAGATGGCG CTGGGCGCAA TGCGCGCCAT TACCGAGTCC 7141 GGGCTGCGCG TTGGTGCGGA TATCTCGGTA GTGGGATACG ACGATACCGA AGACAGCTCA 7201 TGTTATATCC CGCCGTTAAC CACCATCAAA CAGGATTTTC GCCTGCTGGG GCAAACCAGC 7261 GTGGACCGCT TGCTGCAACT CTCTCAGGGC CAGGCGGTGA AGGGCAATCA GCTGTTGCCC 7321 GTCTCACTGG TGAAAAGAAA AACCACCCTG GCGCCCAATA CGCAAACCGC CTCTCCCCGC 7381 GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG 7441 TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT 7501 TATGCTTCCG GCTCGTATGT TGTGTnnnn //