LOCUS       p15a_Kan_MLS_Ros        9202 bp    DNA     circular SYN 19-May-2017
DEFINITION  p15a_Kan_MLS_Rosa26_Short_CRISPRDel_CAG_FNeoStop_WPRE_bgh_pA
ACCESSION   p15a_Kan_MLS_Rosa26_Short_CRISPRDel_CAG_FNeoStop_WPRE_bgh_pA
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 9202)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     Textco/File created by Gene Construction Kit (Textco BioSoftware)
FEATURES             Location/Qualifiers
     CDS             complement(8061..8873)
                     /label="KanR"
     misc_feature    945..1970
                     /label="5' Rosa26 Homology Arm"
     misc_feature    6804..8035
                     /label="3' Rosa26 Homology Arm"
     misc_feature    complement(1..911)
                     /label="p15a origin"
     misc_feature    1998..3698
                     /label="CAG"
     misc_feature    1981..3699
                     /label="Construct:  PCR_RR823_RR824"
     misc_feature    6567..6794
                     /label="bgh_pA"
     misc_feature    complement(2230..2249)
                     /label="RR712"
     misc_feature    complement(2198..2216)
                     /label="RR729"
     misc_feature    complement(6674..6693)
                     /label="RR779"
     misc_feature    complement(2786..2805)
                     /label="RR829"
     misc_feature    3320..3339
                     /label="RR830"
     CDS             3744..4553
                     /label="NeoR"
     misc_feature    4557..5905
     misc_feature    4557..5905
                     /label="Construct:  pCassette_Neo_Stop"
                     /note=">gi|6691170|gb|M77789.2|SYNPUC19V Cloning vector pUC19 comple
                     te sequence"
     misc_feature    4557..5905
                     /label="PBS302 Stop Cassette"
     misc_feature    4557..4585
                     /label="RR819"
     misc_feature    complement(5059..5088)
                     /label="RR800"
     misc_feature    3748..3767
                     /label="RR777"
     misc_feature    5014..5042
                     /label="RR833"
     misc_feature    4208..4227
                     /label="RR778"
     misc_feature    complement(5880..5905)
                     /label="RR820"
     misc_feature    complement(5510..5530)
                     /label="RR863"
     misc_feature    5506..5527
                     /label="RR842"
     misc_feature    3707..3740
     misc_feature    5906..5939
     misc_feature    3707..5939
                     /label="p15a_Amp_CAG_MCS1_bgh_pA.gcc"
     misc_feature    3707..3740
                     /label="FRT"
     misc_feature    5906..5939
                     /label="FRT"
     misc_feature    5966..6557
                     /label="WPRE"
     misc_feature    complement(6333..6352)
                     /label="RR919"
     misc_feature    complement(6532..6557)
                     /label="RR917"
     misc_feature    5966..5990
                     /label="RR916"
     misc_feature    complement(6179..6198)
                     /label="RR920"
     misc_feature    complement(6334..6353)
                     /label="RR919"
     misc_feature    6179..6198
                     /label="RR918"
     misc_feature    complement(6132..6151)
                     /label="RR921"
     misc_feature    complement(6041..6060)
                     /label="RR923"
     misc_feature    5962..5965
     misc_feature    5962..5965
                     /label="p15a_Amp_CAG_F_P_WPRE_bghpA"
     misc_feature    5962..5990
                     /label="RR1091"
     misc_feature    1981..6799
                     /label="p15a_Kan_MLS_Rosa26_Short_CRISPRDel"
ORIGIN
        1 gctagcggag tgtatactgg cttactatgt tggcactgat gagggtgtca gtgaagtgct  
       61 tcatgtggca ggagaaaaaa ggctgcaccg gtgcgtcagc agaatatgtg atacaggata  
      121 tattccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg gcgagcggaa  
      181 atggcttacg aacggggcgg agatttcctg gaagatgcca ggaagatact taacagggaa  
      241 gtgagagggc cgcggcaaag ccgtttttcc ataggctccg cccccctgac aagcatcacg  
      301 aaatctgacg ctcaaatcag tggtggcgaa acccgacagg actataaaga taccaggcgt  
      361 ttccccctgg cggctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca  
      421 ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga cactcagttc cgggtaggca  
      481 gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgaccg ctgcgcctta  
      541 tccggtaact atcgtcttga gtccaacccg gaaagacatg caaaagcacc actggcagca  
      601 gccactggta attgatttag aggagttagt cttgaagtca tgcgccggtt aaggctaaac  
      661 tgaaaggaca agttttggtg actgcgctcc tccaagccag ttacctcggt tcaaagagtt  
      721 ggtagctcag agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt tttcagagca  
      781 agagattacg cgcagaccaa aacgatctca agaagatcat cttattaatc agataaaata  
      841 tttctagatt tcagtgcaat ttatctcttc aaatgtagca cctgaagtca gccccatacg  
      901 atataagttg tATTTCGTCG ACGGAAGAGC GGCGCGCCAA ATCTCCGTTC TGTGAGACAG  
      961 CCGGGTACGA GTCGTGACGC TGGAAGGGGC AAGCGGGTGG TGGGCAGGAA TGCGGTCCGC  
     1021 CCTGCAGCAA CCGGAGGGGG AGGGAGAAGG GAGCGGAAAA GTCTCCACCG GACGCGGCCA  
     1081 TGGCTCGGGG GGGGGGGGGC AGCGGAGGAG CGCTTCCGGC CGACGTCTCG TCGCTGATTG  
     1141 GCTTCTTTTC CTCCCGCCGT GTGTGAAAAC ACAAATGGCG TGTTTTGGTT GGCGTAAGGC  
     1201 GCCTGTCAGT TAACGGCAGC CGGAGTGCGC AGCCGCCGGC AGCCTCGCTC TGCCCACTGG  
     1261 GTGGGGCGGG AGGTAGGTGG GGTGAGGCGA GCTGGACGTG CGGGCGCGGT CGGCCTCTGG  
     1321 CGGGGCGGGG GAGGGGAGGG AGGGTCAGCG AAAGTAGCTC GCGCGCGAGC GGCCGCCCAC  
     1381 CCTCCCCTTC CTCTGGGGGA GTCGTTTTAC CCGCCGCCGG CCGGGCCTCG TCGTCTGATT  
     1441 GGCTCTCGGG GCCCAGAAAA CTGGCCCTTG CCATTGGCTC GTGTTCGTGC AAGTTGAGTC  
     1501 CATCCGCCGG CCAGCGGGGG CGGCGAGGAG GCGCTCCCAG GTTCCGGCCC TCCCCTCGGC  
     1561 CCCGCGCCGC AGAGTCTGGC CGCGCGCCCC TGCGCAACGT GGCAGGAAGC GCGCGCTGGG  
     1621 GGCGGGGACG GGCAGTAGGG CTGAGCGGCT GCGGGGCGGG TGCAAGCACG TTTCCGACTT  
     1681 GAGTTGCCTC AAGAGGGGCG TGCTGAGCCA GACCTCCATC GCGCACTCCG GGGAGTGGAG  
     1741 GGAAGGAGCG AGGGCTCAGT TGGGCTGTTT TGGAGGCAGG AAGCACTTGC TCTCCCAAAG  
     1801 TCGCTCTGAG TTGTTATCAG TAAGGGAGCT GCAGTGGAGT AGGCGGGGAG AAGGCCGCAC  
     1861 CCTTCTCCGG AGGGGGGAGG GGAGTGTTGC AATACCTTTC TGGGAGTTCT CTGCTGCCTC  
     1921 CTGGCTTCTG AGGACCGCCC TGGGCCTGGG AGAATCCCTT CCCCCTCTTC TTTCTTTAAT  
     1981 taagccattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata  
     2041 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc  
     2101 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag  
     2161 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac  
     2221 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg  
     2281 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg  
     2341 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc  
     2401 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca  
     2461 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg  
     2521 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt  
     2581 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc  
     2641 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc  
     2701 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc  
     2761 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag  
     2821 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg  
     2881 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg  
     2941 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc  
     3001 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg  
     3061 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc  
     3121 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc  
     3181 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg  
     3241 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct  
     3301 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg  
     3361 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc  
     3421 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt  
     3481 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg  
     3541 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg  
     3601 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca  
     3661 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attGATGAAG TTCCTATTCt  
     3721 ctagaaaGTA TAGGAACTTC accatgggat cggccattga acaagatgga ttgcacgcag  
     3781 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg  
     3841 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca  
     3901 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc  
     3961 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg  
     4021 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg  
     4081 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta  
     4141 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag  
     4201 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac  
     4261 tgttcgccag gctcaaggcg cgcatgcccg acggcgatga tctcgtcgtg acccatggcg  
     4321 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg  
     4381 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg  
     4441 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg  
     4501 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttcTAATAG TGAGATatta  
     4561 agggttccgg atcctcgggg acaccaaata tggcgatctc ggccttttcg tttcttggag  
     4621 ctgggacatg tttgccatcg atccatctac caccagaacg gccgttagat ctgctgccac  
     4681 cgttgtttcc accgaagaaa ccaccgttgc cgtaaccacc acgacggttg ttgctaaaga  
     4741 agctgccacc gccacggcca ccgttgtagc cgccgttgtt gttattgtag ttgctcatgt  
     4801 tatttctggc acttcttggt tttcctctta agtgaggagg aacataacca ttctcgttgt  
     4861 tgtcgttgat gcttaaattt tgcacttgtt cgctcagttc agccataata tgaaatgctt  
     4921 ttcttgttgt tcttacggaa taccacttgc cacctatcac cacaactaac tttttcccgt  
     4981 tcctccatct cttttatatt ttttttctcg agggatcttt gtgaaggaac cttacttctg  
     5041 tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg taaatataaa  
     5101 atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattcc  
     5161 aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt  
     5221 ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc aacattctac  
     5281 tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag aattgctaag  
     5341 ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta tttacaccac  
     5401 aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatattctg taacctttat  
     5461 aagtaggcat aacagttata atcataacat actgtttttt cttactccac acaggcatag  
     5521 agtgtctgct attaataact atgctcaaaa attgtgtacc tttagctttt taatttgtaa  
     5581 aggggttaat aaggaatatt tgatgtatag tgccttgact agagatcata atcagccata  
     5641 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga  
     5701 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca  
     5761 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt  
     5821 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatctgacat ggtaagtaag  
     5881 cttgggctgc aggtcgaggg acctaGAAGT TCCTATTCtc tagaaaGTAT AGGAACTTCA  
     5941 TCAGGCCTGT TTAAAACATT TAAATAATCA ACCTCTGGAT TACAAAATTT GTGAAAGATT  
     6001 GACTGGTATT CTTAACTATG TTGCTCCTTT TACGCTATGT GGATACGCTG CTTTAATGCC  
     6061 TTTGTATCAT GCTATTGCTT CCCGTATGGC TTTCATTTTC TCCTCCTTGT ATAAATCCTG  
     6121 GTTGCTGTCT CTTTATGAGG AGTTGTGGCC CGTTGTCAGG CAACGTGGCG TGGTGTGCAC  
     6181 TGTGTTTGCT GACGCAACCC CCACTGGTTG GGGCATTGCC ACCACCTGTC AGCTCCTTTC  
     6241 CGGGACTTTC GCTTTCCCCC TCCCTATTGC CACGGCGGAA CTCATCGCCG CCTGCCTTGC  
     6301 CCGCTGCTGG ACAGGGGCTC GGCTGTTGGG CACTGACAAT TCCGTGGTGT TGTCGGGGAA  
     6361 GCTGACGTCC TTTCCATGGC TGCTCGCCTG TGTTGCCACC TGGATTCTGC GCGGGACGTC  
     6421 CTTCTGCTAC GTCCCTTCGG CCCTCAATCC AGCGGACCTT CCTTCCCGCG GCCTGCTGCC  
     6481 GGCTCTGCGG CCTCTTCCGC GTCTTCGCCT TCGCCCTCAG ACGAGTCGGA TCTCCCTTTG  
     6541 GGCCGCCTCC CCGCCTGAAA TAATTCcgac tgtgccttct agttgccagc catctgttgt  
     6601 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta  
     6661 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg  
     6721 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc  
     6781 ggtgggctct atggTTAATT AAAGAAGATG GGCGGGAGTC TTCTGGGCAG GCTTAAAGGC  
     6841 TAACCTGGTG TGTGGGCGTT GTCCTGCAGG GGAATTGAAC AGGTGTAAAA TTGGAGGGAC  
     6901 AAGACTTCCC ACAGATTTTC GGTTTTGTCG GGAAGTTTTT TAATAGGGGC AAATAAGGAA  
     6961 AATGGGAGGA TAGGTAGTCA TCTGGGGTTT TATGCAGCAA AACTACAGGT TATTATTGCT  
     7021 TGTGATCCGC CTCGGAGTAT TTTCCATCGA GGTAGATTAA AGACATGCTC ACCCGAGTTT  
     7081 TATACTCTCC TGCTTGAGAT CCTTACTACA GTATGAAATT ACAGTGTCGC GAGTTAGACT  
     7141 ATGTAAGCAG AATTTTAATC ATTTTTAAAG AGCCCAGTAC TTCATATCCA TTTCTCCCGC  
     7201 TCCTTCTGCA GCCTTATCAA AAGGTATTTT AGAACACTCA TTTTAGCCCC ATTTTCATTT  
     7261 ATTATACTGG CTTATCCAAC CCCTAGACAG AGCATTGGCA TTTTCCCTTT CCTGATCTTA  
     7321 GAAGTCTGAT GACTCATGAA ACCAGACAGA TTAGTTACAT ACACCACAAA TCGAGGCTGT  
     7381 AGCTGGGGCC TCAACACTGC AGTTCTTTTA TAACTCCTTA GTACACTTTT TGTTGATCCT  
     7441 TTGCCTTGAT CCTTAATTTT CAGTGTCTAT CACCTCTCCC GTCAGGTGGT GTTCCACATT  
     7501 TGGGCCTATT CTCAGTCCAG GGAGTTTTAC AACAATAGAT GTATTGAGAA TCCAACCTAA  
     7561 AGCTTAACTT TCCACTCCCA TGAATGCCTC TCTCCTTTTT CTCCATTTAT AAACTGAGCT  
     7621 ATTAACCATT AATGGTTTCC AGGTGGATGT CTCCTCCCCC AATATTACCT GATGTATCTT  
     7681 ACATATTGCC AGGCTGATAT TTTAAGACAT TAAAAGGTAT ATTTCATTAT TGAGCCACAT  
     7741 GGTATTGATT ACTGCTTACT AAAATTTTGT CATTGTACAC ATCTGTAAAA GGTGGTTCCT  
     7801 TTTGGAATGC AAAGTTCAGG TGTTTGTTGT CTTTCCTGAC CTAAGGTCTT GTGAGCTTGT  
     7861 ATTTTTTCTA TTTAAGCAGT GCTTTCTCTT GGACTGGCTT GACTCATGGC ATTCTACACG  
     7921 TTATTGCTGG TCTAAATGTG ATTTTGCCAA GCTTCTTCAG GACCTATAAT TTTGCTTGAC  
     7981 TTGTAGCCAA ACACAAGTAA AATGATTAAG CAACAAATGT ATTTGTGAAG CTTGGTCCAC  
     8041 CACGCTCGGA GGGCCTGTTA GAAAAACTCA TCGAGCATCA AATGAAACTG CAATTTATTC  
     8101 ATATCAGGAT TATCAATACC ATATTTTTGA AAAAGCCGTT TCTGTAATGA AGGAGAAAAC  
     8161 TCACCGAGGC AGTTCCATAG GATGGCAAGA TCCTGGTATC GGTCTGCGAT TCCGACTCGT  
     8221 CCAACATCAA TACAACCTAT TAATTTCCCC TCGTCAAAAA TAAGGTTATC AAGTGAGAAA  
     8281 TCACCATGAG TGACGACTGA ATCCGGTGAG AATGGCAAAA GCTTATGCAT TTCTTTCCAG  
     8341 ACTTGTTCAA CAGGCCAGCC ATTACGCTCG TCATCAAAAT CACTCGCATC AACCAAACCG  
     8401 TTATTCATTC GTGATTGCGC CTGAGCGAGA CGAAATACGC GATCGCTGTT AAAAGGACAA  
     8461 TTACAAACAG GAATCGAATG CAACCGGCGC AGGAACACTG CCAGCGCATC AACAATATTT  
     8521 TCACCTGAAT CAGGATATTC TTCTAATACC TGGAATGCTG TTTTCCCGGG GATCGCAGTG  
     8581 GTGAGTAACC ATGCATCATC AGGAGTACGG ATAAAATGCT TGATGGTCGG AAGAGGCATA  
     8641 AATTCCGTCA GCCAGTTTAG TCTGACCATC TCATCTGTAA CATCATTGGC AACGCTACCT  
     8701 TTGCCATGTT TCAGAAACAA CTCTGGCGCA TCGGGCTTCC CATACAATCG ATAGATTGTC  
     8761 GCACCTGATT GCCCGACATT ATCGCGAGCC CATTTATACC CATATAAATC AGCATCCATG  
     8821 TTGGAATTTA ATCGCGGCCT CGAGCAAGAC GTTTCCCGTT GAATATGGCT CATAACACCC  
     8881 CTTGTATTAC TGTTTATGTA AGCAGACAGT TTTATTGTTC ATGATGATAT ATTTTTATCT  
     8941 TGTGCAATGT AACATCAGAG ATTTTGAGAC ACAACGTGGC TTTGTTGAAT AAATCGAACT  
     9001 TTTGCTGAGT TGAAGGATCA GATCACGCAT CTTCCCGACA ACGCAGACCG TTCCGTGGCA  
     9061 AAGCAAAAGT TCAAAATCAC CAACTGGTCC ACCTACAACA AAGCTCTCAT CAACCGTGGC  
     9121 TCCCTCACTT TCTGGCTGGA TGATGGGGCG ATTCAGGCCT GGTATGAGTC AGCAACACCT  
     9181 TCTTCACGAG GCAGACCTCa gc 
//
