LOCUS       TetO-FUW-Ascl1-P        9762 bp    DNA     circular     11-FEB-2011
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|581623824|
COMMENT     VNTDBDATE|581627454|
COMMENT     LSOWNER|
COMMENT     VNTNAME|TetO-FUW-Ascl1-Puro|
COMMENT     VNTAUTHORNAME|Samuele Marro|
COMMENT     VNTAUTHOREML|smarro@stanford.edu|
COMMENT     VNTOAUTHORNAME|Ruth Foreman|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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FEATURES             Location/Qualifiers
     promoter        6930..7534
                     /dnas_title="CMV promoter/enhancer"
                     /vntifkey="29"
                     /label=CMV\promoter/enhancer
     LTR             7553..8227
                     /dnas_title="5' HIVR-U5"
                     /vntifkey="19"
                     /label=5'\HIVR-U5
     misc_binding    8251..9108
                     /dnas_title="HIV rev response element"
                     /vntifkey="20"
                     /label=HIV\rev\response\element
     misc_binding    9134..9311
                     /dnas_title="HIV flap/nuclear import signal"
                     /vntifkey="20"
                     /label=HIV\flap/nuclear\import\signal
     misc_feature    1410..1998
                     /dnas_title="WRE"
                     /vntifkey="21"
                     /label=WRE
     LTR             2014..2709
                     /dnas_title="HIV delU3RdelU5"
                     /vntifkey="19"
                     /label=HIV\delU3RdelU5
     misc_marker     3889..4262
                     /dnas_title="ZEO"
                     /vntifkey="22"
                     /label=ZEO
     misc_marker     5728..6586
                     /dnas_title="AMP"
                     /vntifkey="22"
                     /label=AMP
     misc_feature    9314..9758
                     /dnas_title="TetO"
                     /vntifkey="21"
                     /label=TetO
     CDS             13..705
                     /vntifkey="4"
                     /label=Aslc1
     misc_feature    712..765
                     /vntifkey="21"
                     /label=T2A_ncbi
     misc_feature    772..1371
                     /vntifkey="21"
                     /label=Puro
     misc_feature    7..12
                     /vntifkey="21"
                     /label=Kozak
BASE COUNT     2366 a      2542 c      2603 g      2251 t 
ORIGIN
        1 gaattcgcca ccatggagag ctctggcaag atggagagtg gagccggcca gcagccgcag 
       61 cccccgcagc ccttcctgcc tcccgcagcc tgcttctttg cgaccgcggc ggcggcggca 
      121 gcggcggcgg ccgcggcagc tcagagcgcg cagcagcaac agccgcaggc gccgccgcag 
      181 caggcgccgc agctgagccc ggtggccgac agccagccct cagggggcgg tcacaagtca 
      241 gcggccaagc aggtcaagcg ccagcgctcg tcctctccgg aactgatgcg ctgcaaacgc 
      301 cggctcaact tcagcggctt cggctacagc ctgccacagc agcagccggc cgccgtggcg 
      361 cgccgcaacg agcgcgagcg caaccgggtc aagttggtca acctgggttt tgccaccctc 
      421 cgggagcatg tccccaacgg cgcggccaac aagaagatga gcaaggtgga gacgctgcgc 
      481 tcggcggtcg agtacatccg cgcgctgcag cagctgctgg acgagcacga cgcggtgagc 
      541 gctgcctttc aggcgggcgt cctgtcgccc accatctccc ccaactactc caacgacttg 
      601 aactctatgg cgggttctcc ggtctcgtcc tactcctccg acgagggatc ctacgaccct 
      661 cttagcccag aggaacaaga gctgctggac tttaccaact ggttctctag agagggcagg 
      721 ggaagtcttc taacatgcgg ggacgtggag gaaaatcccg gcccactcga gatgaccgag 
      781 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc 
      841 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc 
      901 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag 
      961 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa 
     1021 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg 
     1081 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg 
     1141 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc 
     1201 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc 
     1261 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag 
     1321 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg attaattaag 
     1381 ctagctaatt cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa 
     1441 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa 
     1501 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat 
     1561 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt 
     1621 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc 
     1681 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc 
     1741 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg 
     1801 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga 
     1861 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc 
     1921 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc 
     1981 tttgggccgc ctccccgcat cgataccgtc gacctcgaga cctagaaaaa catggagcaa 
     2041 tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg 
     2101 aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg 
     2161 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact 
     2221 cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg 
     2281 attggcagaa ctacacacca gggccaggga tcagatatcc actgaccttt ggatggtgct 
     2341 acaagctagt accagttgag caagagaagg tagaagaagc caatgaagga gagaacaccc 
     2401 gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtattagagt 
     2461 ggaggtttga cagccgccta gcatttcatc acatggcccg agagctgcat ccggactgta 
     2521 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc 
     2581 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt 
     2641 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta 
     2701 gcagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct 
     2761 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt 
     2821 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg 
     2881 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg 
     2941 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat 
     3001 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg 
     3061 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc 
     3121 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga 
     3181 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt 
     3241 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat 
     3301 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat 
     3361 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa 
     3421 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct 
     3481 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa 
     3541 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa 
     3601 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt 
     3661 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct 
     3721 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc 
     3781 tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc 
     3841 ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca tggccaagtt 
     3901 gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac 
     3961 cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga 
     4021 cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc 
     4081 ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac 
     4141 gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg 
     4201 ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga 
     4261 ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg 
     4321 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt 
     4381 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat 
     4441 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact 
     4501 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc 
     4561 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg 
     4621 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat 
     4681 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg 
     4741 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct 
     4801 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 
     4861 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 
     4921 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 
     4981 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 
     5041 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 
     5101 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 
     5161 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 
     5221 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 
     5281 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 
     5341 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 
     5401 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 
     5461 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 
     5521 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 
     5581 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 
     5641 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 
     5701 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 
     5761 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 
     5821 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 
     5881 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 
     5941 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 
     6001 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg 
     6061 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 
     6121 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 
     6181 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 
     6241 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 
     6301 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc 
     6361 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 
     6421 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 
     6481 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 
     6541 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 
     6601 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 
     6661 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt 
     6721 cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat 
     6781 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc 
     6841 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg 
     6901 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat 
     6961 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat 
     7021 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac 
     7081 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc 
     7141 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg 
     7201 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat 
     7261 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc 
     7321 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt 
     7381 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac 
     7441 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc 
     7501 ggtaggcgtg tacggtggga ggtctatata agcagcgcgt tttgcctgta ctgggtctct 
     7561 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 
     7621 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 
     7681 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc 
     7741 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg 
     7801 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt 
     7861 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg 
     7921 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa 
     7981 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg 
     8041 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca 
     8101 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa 
     8161 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa 
     8221 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag 
     8281 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt 
     8341 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg 
     8401 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac 
     8461 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct 
     8521 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct 
     8581 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg 
     8641 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa 
     8701 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa 
     8761 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa 
     8821 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac 
     8881 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag 
     8941 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc 
     9001 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga 
     9061 aggtggagag agagacagag acagatccat tcgattagtg aacggatcgg cactgcgtgc 
     9121 gccaattctg cagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg 
     9181 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag 
     9241 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag 
     9301 atccagtttg gttactcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg 
     9361 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc 
     9421 agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga 
     9481 aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc 
     9541 cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa 
     9601 aagtgaaagt cgagctcggt acccgggtcg agtaggcgtg tacggtggga ggcctatata 
     9661 agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac 
     9721 ctccatagaa gacaccggga ccgatccagc ctccgcggcc cc 
//
