LOCUS       Exported                8793 bp ds-DNA     circular SYN 05-MAR-2019
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pNOC-NLUC.dna
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 5, 2019 from SnapGene 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8793
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     promoter        1..798
                     /label=LDSP promoter
                     /note="color: #ffffff"
     CDS             799..1824
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /note="color: #993366"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     terminator      1842..2018
                     /label=35S terminator
                     /note="color: #ffffff"
     protein_bind    2068..2192
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
                     /note="color: #31849b"
     CDS             2301..2960
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /note="color: #993366"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             3302..3607
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /note="color: #993366"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(3648..3772)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
                     /note="color: #31849b; direction: LEFT"
     CDS             3792..4307
                     /codon_start=1
                     /label=Nlux
                     /note="color: #993366"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILAG"
     CDS             4308..4334
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="color: #993366"
                     /translation="YPYDVPDYA"
     terminator      4342..6009
                     /label=LDSP terminator
                     /note="color: #ffffff"
     rep_origin      complement(6468..7056)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(7227..8087)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #993366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8088..8192)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     rep_origin      complement(8270..8725)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
ORIGIN
        1 gtaggtctct aagatggagt ggatggagga ggaggcgagc gtagcagcaa gcgtgagtta
       61 tacagccagg cacatgtcgc aatccttcgg tctcgggctt aaaatccacg cactaatcac
      121 gctgggccat gcaaagagca atgccgaggc ccaccacaca aaacgctgtg tcgcgcgttg
      181 cggcctgaag cttcatactt cttagtcgcc gccaaaaggg ctcgagagac gagacccgtt
      241 ggcatgaccg atgttgttcg acgcggtttg cttcgtcaca gtcgacgtga ttcaggaatc
      301 tggagcctgc agatcatttt tttcagcctg atatcgttct tttccactga gaaccatcag
      361 accacctttt cttccattgt gtgaaggagt aggagttgcc gtgctgcttt gtgggagaca
      421 tctgcgatgg tgaccagcct cccgtcgtct ggtcgacgtg acgagcctct tcactgttct
      481 tcgacggaga gacgcaagcg agacggctct agaccttttg gacacgcatt ctgtgtgtga
      541 actagtggac agtgatacca cgtctgaaag ctcaccactg cccatggtgc agctacttgt
      601 cacaaagttt tgactccgtc ggtatcacca ttcgcgctcg tgtgcctggt tgttccgcca
      661 cgccggcctg ccccggggcg gggcaatatt ctaaaatctc acgcaaaaca ccgcacttac
      721 ccctcacaca tattcgtgat agaccaccac caatctcagc ccgcatcaac acaggagggc
      781 cccggggggc aataagatat gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt
      841 ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct
      901 cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc
      961 gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt
     1021 ccggaagtgc ttgacattgg ggaattcagc gagagcctga cctattgcat ctcccgccgt
     1081 gcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg
     1141 gtcgcggagg ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc
     1201 ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt
     1261 gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc
     1321 gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc
     1381 gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc
     1441 attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc
     1501 tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg
     1561 gagcttgcag gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc
     1621 tatcagagct tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac
     1681 gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg
     1741 gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc
     1801 actcgtccga gggcaaagga atagagtaga tgccgaccgg agtccgcaaa aatcaccagt
     1861 ctctctctac aaatctatct ctctctattt ttctccagaa taatgtgtga gtagttccca
     1921 gataagggaa ttagggttct tatagggttt cgctcatgtg ttgagcatat aagaaaccct
     1981 tagtatgtat ttgtatttgt aaaatacttc tatcaataaa atttctaatt cctaaaacca
     2041 aaatccagtg acctgcaggc gatatcaaca agtttgtaca aaaaagctga acgagaaacg
     2101 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa
     2161 tactgtaaaa cacaacatat ccagtcacta tggcggccgc attaggcacc ccaggcttta
     2221 cactttatgc ttccggctcg tataatgtgt ggattttgag ttaggatccg gcgagatttt
     2281 caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat
     2341 cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata
     2401 accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca
     2461 agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc
     2521 gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg
     2581 ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc
     2641 ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt
     2701 tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca
     2761 ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg
     2821 gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg
     2881 ccgtctgtga tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg
     2941 agtggcaggg cggggcgtaa agatctggat ccggcttact aaaagccaga taacagtatg
     3001 cgtatttgcg cgctgatttt tgcggtataa gaatatatac tgatatgtat acccgaagta
     3061 tgtcaaaaag aggtgtgcta tgaagcagcg tattacagtg acagttgaca gcgacagcta
     3121 tcagttgctc aaggcatata tgatgtcaat atctccggtc tggtaagcac aaccatgcag
     3181 aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg aaaatcagga agggatggct
     3241 gaggtcgccc ggtttattga aatgaacggc tcttttgctg acgagaacag ggactggtga
     3301 aatgcagttt aaggtttaca cctataaaag agagagccgt tatcgtctgt ttgtggatgt
     3361 acagagtgat attattgaca cgcccgggcg acggatggtg atccccctgg ccagtgcacg
     3421 tctgctgtca gataaagtct cccgtgaact ttacccggtg gtgcatatcg gggatgaaag
     3481 ctggcgcatg atgaccaccg atatggccag tgtgccggtc tccgttatcg gggaagaagt
     3541 ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg
     3601 aatataaatg tcaggctccc ttatacacag ccagtctgca ggtcgaccat agtgactgga
     3661 tatgttgtgt tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat
     3721 tgatatttat atcattttac gtttctcgtt cagctttctt gtacaaagtg gttgatagct
     3781 tggcgcgcct catggtgttt actctcgagg acttcgtggg cgactggcgg cagacagctg
     3841 gctataacct cgaccaggtg ctggagcagg gcggtgtgtc ctcgcttttc cagaacctgg
     3901 gcgtctccgt gaccccaatc cagcgcatcg tcctgagcgg cgagaacggc ctgaagatcg
     3961 acatccacgt catcattccc tacgagggcc tgagcgggga ccaaatgggc cagatcgaga
     4021 agatcttcaa ggttgtgtac cccgtggacg atcaccactt caaggtgata ctgcactacg
     4081 ggacgctggt catcgacggt gtgacgccga acatgattga ctacttcggc cgtccttacg
     4141 agggcattgc cgtgttcgac ggcaagaaga tcacggtgac cggcaccctg tggaacggca
     4201 ataagatcat tgacgagcgg ctgatcaacc cggatggctc gctgttgttc cgcgtgacca
     4261 tcaacggggt aaccggctgg cgcctgtgcg agcgcatcct ggccggatac ccctacgacg
     4321 tgcccgacta cgcgtgagct cgaaagatcc aagagagacg agtagagatt ttttttttgg
     4381 gattgatgtt tgtcgttctt tgagttgtcg tcgagttacg ccttttgtaa gaatgttccg
     4441 caggagagga ggaggatggg catgagtgag ggtgagaggg cttgcccgct ttttttttta
     4501 aaaacgctgc tcttcgtggt tgtcaaacaa accccccata gaaacgattt tgttacggtg
     4561 cggtccagac gtcacttgaa tggctccgcg gaaaggccag ggagggaagg ggggagggag
     4621 gaaacatgaa acatgttgaa cggctcaaca gggtttgggg gacaagagag gtagcgccct
     4681 gatggactgc tccctcccct cctttccctc aatgtctcat tcatccatgc ttcccccttc
     4741 tctctctccc ctccgttcca tcccccgcgg gcgtggtagt ggcgtgatgg gatccactaa
     4801 aatgtacgtg taagaaaagc cggtgagctt acgcttttgt gaaagtggga gtacgagtgt
     4861 tgtgtgtgtg tgtagtggtt tcagacccca gacagaggcg aagcagaaaa agcagacgat
     4921 gaagacgacg aagaaatgag cagtctattt ttatcgtgga aacagaagag gtgatatcgt
     4981 ctcgttcttt gttatcacct accccgcgtg catgtacatg cagccttttt attttgtaat
     5041 ctttcccgaa aaatcaaccg ccacctcccc cccgccttct ctcacccatc atcttctcct
     5101 gtttatcttc tactttacac tagatcgcat ggcacatctc cctcgcaatc catcggtgca
     5161 accatcatcg atcccactcc tccctccctc cctccctccc tcctcgcatc tcttctaaga
     5221 aatccgctag ctgcgaacgc agctcaccta cctcatttat cagagcctcg attcggtcct
     5281 tttccttctc ccctacctcg ccgcaaacag caacagaagc aggagcagca ggagcagcgg
     5341 cacctgtgtc aacagcacaa gggcaagtac agacagaaga cgaggcggag gaggaggaag
     5401 acgatgtgat gtcattggag ctggctaaac tattagccgt tgctctcacc gcctttcccc
     5461 aaaactcttc cacccatcgt gtcactcctt ctatcagggg aaccatccct cttttcctcc
     5521 ccaccttctt ccccttgctc ttcttcttcc tttgtctctt tgttccccct cctctcttcg
     5581 cctcctcctc cgcatcgtcc tctcctttta tcaagtcttg cgttgcaaag gaagcaagct
     5641 ctttgtgaag gaccgcgcaa gggataggag agtggagagt gaggtgatgc agacaaaccg
     5701 aggattcccg ggcgtcagat aacctaaaat cgactacttc ttgaggtgga ggaggagggg
     5761 gaggagacgg agaataagtg tgtgtagaaa tatagcttgc tgcctctgct tccatcttcg
     5821 ataaaattgc tgttgctgtt gctgttgcgg ctgatatctg ctgcggctgc tgctgcacct
     5881 cctgaacgaa gactgcagcc gcgtcttcac cctcttggac tactctgccc tcctcccttg
     5941 cctttacaac tcgatcgacg agattcgtgc cagtgctgtc atcatcattg gaaagagcaa
     6001 cagcatcacc ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct
     6061 gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg
     6121 ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata
     6181 gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag gctgcattaa
     6241 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     6301 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     6361 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     6421 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     6481 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     6541 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     6601 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     6661 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     6721 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     6781 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     6841 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     6901 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     6961 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     7021 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     7081 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     7141 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     7201 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     7261 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     7321 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     7381 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     7441 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     7501 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     7561 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     7621 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     7681 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     7741 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     7801 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     7861 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     7921 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     7981 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     8041 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     8101 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     8161 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgat
     8221 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcagga aattgtaagc
     8281 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
     8341 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
     8401 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
     8461 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
     8521 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
     8581 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
     8641 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
     8701 cttaatgcgc cgctacaggg cgcgtccatt cgccattcag gctgcgcaac tgttgggaag
     8761 ggcgatcggt gcgggcctct tcgctattac gcc
//
