LOCUS       Exported               10996 bp ds-DNA     circular SYN 05-MAR-2019
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pNSA-FLUC.dna
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 5, 2019 from SnapGene 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10996
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     promoter        745..1737
                     /label=CCMP537 EF promoter
                     /note="color: #ffffff"
     CDS             1753..2127
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /note="color: #993366"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     terminator      2191..2831
                     /label=LDSP terminator
                     /note="color: #ffffff"
     protein_bind    2921..3045
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
                     /note="color: #31849b"
     promoter        3070..3100
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
                     /note="color: #ffffff"
     CDS             3154..3813
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /note="color: #993366"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             4155..4460
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /note="color: #993366"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(4501..4625)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
                     /note="color: #31849b; direction: LEFT"
     CDS             4645..6312
                     /codon_start=1
                     /label=Flux
                     /note="color: #993366"
                     /translation="MGGSGEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIA
                     FTDAHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGV
                     AVAPANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQ
                     GFQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
                     FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSL
                     QDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGI
                     RQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGP
                     MIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAEL
                     ESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLR
                     GGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAVSS"
     CDS             6313..6339
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="color: #993366"
                     /translation="YPYDVPDYA"
     terminator      6349..8016
                     /label=LDSP terminator
                     /note="color: #ffffff"
     promoter        complement(8121..8139)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(8157..8173)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    8181..8197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(8205..8235)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="color: #ffffff; direction: LEFT"
     protein_bind    8250..8271
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(8559..9147)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(9318..10178)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #993366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10179..10283)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     rep_origin      complement(10361..10816)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     primer_bind     10957..10973
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat
       61 atcactagag tatagctaca tggtagctag tagtcgtcaa aggaaataaa gacatagcgt
      121 aaagtataca aaatgacttc cgcggcgcgg gagcggcagg cagcacttgc aaaacgaagc
      181 ccaatgggcc agattacggt caacctcgtc attttattac ggtcgtcagc aagagcggtt
      241 tagaccgtcg ccggcacggg tggctcccga tattcttggc tttcgtgggt ggaggcggcg
      301 tgtgactcct catctttaag tagtggtcgc ttttgctgca tgcttttgat cacctcgtga
      361 ttgatggctg gattctcagc tgtcttctga catctttggg tgcatgggtt gaccggtgtc
      421 gcgggaggca gaccctgttc cgtggaggtg tgtggctgcg gatgcggcga cacggaagaa
      481 gggcgtgcat cacttctctc ttagtcgatt tacgatgcgc ttctgcgcac agctcattca
      541 cccacccgcc accactcctc ttgcacatgt atgcaggtgt cgggccaagt tagtcgcgcg
      601 ctgagtggcc cgaaccccgc atgtggtagt ggaatgcgtg gtcggcagaa gccttgagca
      661 cagcgaaaga agaaaacaag ataagcaggg gatggctgcg gtttgcgcgt gcctttgccg
      721 cgcagcccgt cacaacaata cgtacgatcc ttctgattgt ttgtcgctcg gcagaaaatg
      781 aggagtctag ctctgcaaca caaagaaatt cacaaatgta acaagactct gtgccattat
      841 ttcttatttc ttcctctgtt gtcccaatcg gaaaagctag ggccgtgggg tggccgtaat
      901 cccagacatt ccgggcgctg cctcgccaac gggtggctcc cgaaggactt ggctttcgtg
      961 ggcagggagg gccctgcgct gggttttaga cgtgccgcga gtgctgcctt gattcgaggt
     1021 acacgttaga gatatgaggc acatagcagt tcgctggcac ccccgcgtgt aggcgtgtcc
     1081 tcatccgtgt ttggtttggt acgtcggtta ccggaggggc ctgtgtggct gtcaagcgcg
     1141 acgaagacgg gtgagtgttg cctcgtgcct ccaggtgctt tgatgacgcg cattgcgcgt
     1201 ttcgcttatc caaagaccgc aatcactctc tgaccatgca atcaggttga tagggacgtc
     1261 aaagtgcatt ttatggctta atgctggtcg cgcgggtgta catgtggcgt ctggacggga
     1321 acctcagaac gaagaaaaca agatagggat ggctaacagt tgcacacgtc cctcgtgtgg
     1381 ctgactgaaa gacgtttgcg ccacattaat gtgtaaacat attgagctag tcttacagtt
     1441 attcaggagg aggacaaaat taagtcgtcc gaccgtgtgt tatctaggaa gccatgacgc
     1501 gagacatgtg cctagtatct ctagtacggc tgcgtcttct ttcctccctc tttgaaagga
     1561 agccgtcggt gtccgcgatt ctattctatg cgaaagctgt ggaagatgca ttagtcgcgc
     1621 tttccttgat agttgaccgt cagtggctga ttcagggaaa tcctcacatc atcctgtctt
     1681 tctcacacga cagcctcttc tttcggaata ttcccctcaa cacctaatct gtaaaccctc
     1741 gagcagctga ccatggccaa gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc
     1801 gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt cgtggaggac
     1861 gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt ccaggaccag
     1921 gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc
     1981 gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc catgaccgag
     2041 atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg caactgcgtg
     2101 cacttcgtgg ccgaggagca ggactgacaa ttgggtcacg aactccagca gggctagcgt
     2161 acgcgtacga gagctccgat gccccaattg gaaagatcca agagagacga gtagagattt
     2221 tttttttggg attgatgttt gtcgttcttt gagttgtcgt cgagttacgc cttttgtaag
     2281 aatgttccgc aggagaggag gaggatgggc atgagtgagg gtgagagggc ttgcccgctt
     2341 ttttttttaa aaacgctgaa gacgtggttg tcaaacaaac cccccataga aacgattttg
     2401 ttacggtgcg gtccagacgt cacttgaatg gctccgcgga aaggccaggg agggaagggg
     2461 ggagggagga aacatgaaac atgttgaacg gctcaacagg gtttggggga caagagaggt
     2521 agcgccctga tggactgctc cctcccctcc tttccctcaa tgtctcattc atccatgctt
     2581 cccccttctc tctctcccct ccgttccatc ccccgcgggc gtggtagtgg cgtgatggga
     2641 tccactaaaa tgtacgtgta agaaaagccg gtgagcttac gcttttgtga aagtgggagt
     2701 acgagtgttg tgtgtgtgtg tagtggtttc agaccccaga cagaggcgaa gcagaaaaag
     2761 cagacgatga agacgacgaa gaaatgagca gtctattttt atcgtggaaa cagaagaggt
     2821 gatatcgtct cctgcaggca tgcaagctga tccactagag gccatggcgg ccgcactagt
     2881 ggatcccccg ggatgcatga attcaagctt atcgatatca acaagtttgt acaaaaaagc
     2941 tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa
     3001 acagactaca taatactgta aaacacaaca tatccagtca ctatggcggc cgcattaggc
     3061 accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt gagttaggat
     3121 ccggcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc
     3181 accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct
     3241 caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag
     3301 aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct
     3361 catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac
     3421 ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac
     3481 cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa
     3541 aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc
     3601 tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc
     3661 gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt
     3721 caggttcatc atgccgtctg tgatggcttc catgtcggca gaatgcttaa tgaattacaa
     3781 cagtactgcg atgagtggca gggcggggcg taaagatctg gatccggctt actaaaagcc
     3841 agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata tactgatatg
     3901 tatacccgaa gtatgtcaaa aagaggtgtg ctatgaagca gcgtattaca gtgacagttg
     3961 acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg gtctggtaag
     4021 cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca
     4081 ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg ctgacgagaa
     4141 cagggactgg tgaaatgcag tttaaggttt acacctataa aagagagagc cgttatcgtc
     4201 tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg gtgatccccc
     4261 tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg gtggtgcata
     4321 tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg gtctccgtta
     4381 tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc
     4441 tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct gcaggtcgac
     4501 catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc
     4561 taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt cttgtacaaa
     4621 gtggttgata gcttggcgcg cctcatgggc gggtccggcg aggacgcaaa gaatatcaag
     4681 aaaggccctg caccgttcta ccctttggag gacgggaccg cgggagagca gcttcacaag
     4741 gctatgaaac gttacgctct tgttccgggc acgatcgcat tcacggatgc ccacatcgag
     4801 gtcgacatta catatgctga gtacttcgag atgtccgtgc gccttgccga agctatgaag
     4861 cgttacgggc tgaacacgaa ccaccgcatc gtggtctgtt cagagaattc cctacagttc
     4921 tttatgcccg tgttgggggc ccttttcatt ggcgtcgccg tggcccccgc caacgacatt
     4981 tataacgagc gcgagctgct caacagcatg gggatttcgc agccgactgt ggtcttcgtt
     5041 tccaagaaag gtttgcaaaa gatattgaat gtacagaaga aactgcccat tatccagaag
     5101 attatcatta tggactctaa gaccgattac cagggattcc agtcgatgta cactttcgtc
     5161 acgagccatc tcccacccgg gttcaacgag tacgacttcg tgccggagag cttcgaccgc
     5221 gacaagacaa tcgcccttat tatgaactct agtggtagca ccggtttgcc caagggcgtg
     5281 gcgctgcccc atcgtaccgc ctgcgtgcgc ttctcccatg cgcgtgatcc tatcttcggc
     5341 aaccagatca ttccggacac cgcgattctt tcggtcgtgc cgtttcacca tggatttggc
     5401 atgtttacga ctttgggcta cctcatctgc ggcttccggg tggtcctgat gtaccggttt
     5461 gaggaagagc tgtttttgcg cagtctgcag gactacaaga tccagtccgc gctgctcgtc
     5521 cctacactct tttctttctt tgctaagtcc accttaatcg acaagtacga tctgtcaaac
     5581 ctccacgaga tcgcctccgg aggtgcgccc ttatctaagg aagtcgggga ggctgtcgcg
     5641 aagagatttc acctgcccgg catcaggcag ggatatggcc tgaccgagac cacttccgcc
     5701 atcttgatca cgcccgaggg tgacgataaa ccaggcgccg tggggaaagt ggtccctttt
     5761 ttcgaagcaa aggtcgttga cctggacacc ggcaagacgc tcggggtgaa ccaacgaggt
     5821 gagttgtgcg tgcgcggccc tatgattatg agcgggtacg tgaacaatcc cgaagccacc
     5881 aacgcactca ttgataagga cggatggcta cacagcggtg atatcgccta ctgggatgag
     5941 gacgagcact tcttcatcgt agaccggctg aagtcgctca tcaagtacaa ggggtatcag
     6001 gtggcccctg cggagttgga atctattctg ctccaacacc ctaatatctt cgacgccggc
     6061 gtggccggct tgcccgacga tgacgcgggc gagcttcccg ccgcggttgt ggtcctggag
     6121 cacggcaaga cgatgaccga gaaggagatc gtagactacg tggcttccca agtgactacg
     6181 gcgaagaagt tgaggggtgg agtagtgttc gtggatgagg tccccaaggg gctcaccggt
     6241 aagctcgatg cccgtaagat ccgagagatc ttgattaagg ctaagaaggg gggcaagatc
     6301 gccgtgtcgt cgtatcccta cgatgttccc gattatgcct aagagctcga aagatccaag
     6361 agagacgagt agagattttt tttttgggat tgatgtttgt cgttctttga gttgtcgtcg
     6421 agttacgcct tttgtaagaa tgttccgcag gagaggagga ggatgggcat gagtgagggt
     6481 gagagggctt gcccgctttt ttttttaaaa acgctgctct tcgtggttgt caaacaaacc
     6541 ccccatagaa acgattttgt tacggtgcgg tccagacgtc acttgaatgg ctccgcggaa
     6601 aggccaggga gggaaggggg gagggaggaa acatgaaaca tgttgaacgg ctcaacaggg
     6661 tttgggggac aagagaggta gcgccctgat ggactgctcc ctcccctcct ttccctcaat
     6721 gtctcattca tccatgcttc ccccttctct ctctcccctc cgttccatcc cccgcgggcg
     6781 tggtagtggc gtgatgggat ccactaaaat gtacgtgtaa gaaaagccgg tgagcttacg
     6841 cttttgtgaa agtgggagta cgagtgttgt gtgtgtgtgt agtggtttca gaccccagac
     6901 agaggcgaag cagaaaaagc agacgatgaa gacgacgaag aaatgagcag tctattttta
     6961 tcgtggaaac agaagaggtg atatcgtctc gttctttgtt atcacctacc ccgcgtgcat
     7021 gtacatgcag cctttttatt ttgtaatctt tcccgaaaaa tcaaccgcca cctccccccc
     7081 gccttctctc acccatcatc ttctcctgtt tatcttctac tttacactag atcgcatggc
     7141 acatctccct cgcaatccat cggtgcaacc atcatcgatc ccactcctcc ctccctccct
     7201 ccctccctcc tcgcatctct tctaagaaat ccgctagctg cgaacgcagc tcacctacct
     7261 catttatcag agcctcgatt cggtcctttt ccttctcccc tacctcgccg caaacagcaa
     7321 cagaagcagg agcagcagga gcagcggcac ctgtgtcaac agcacaaggg caagtacaga
     7381 cagaagacga ggcggaggag gaggaagacg atgtgatgtc attggagctg gctaaactat
     7441 tagccgttgc tctcaccgcc tttccccaaa actcttccac ccatcgtgtc actccttcta
     7501 tcaggggaac catccctctt ttcctcccca ccttcttccc cttgctcttc ttcttccttt
     7561 gtctctttgt tccccctcct ctcttcgcct cctcctccgc atcgtcctct ccttttatca
     7621 agtcttgcgt tgcaaaggaa gcaagctctt tgtgaaggac cgcgcaaggg ataggagagt
     7681 ggagagtgag gtgatgcaga caaaccgagg attcccgggc gtcagataac ctaaaatcga
     7741 ctacttcttg aggtggagga ggagggggag gagacggaga ataagtgtgt gtagaaatat
     7801 agcttgctgc ctctgcttcc atcttcgata aaattgctgt tgctgttgct gttgcggctg
     7861 atatctgctg cggctgctgc tgcacctcct gaacgaagac tgcagccgcg tcttcaccct
     7921 cttggactac tctgccctcc tcccttgcct ttacaactcg atcgacgaga ttcgtgccag
     7981 tgctgtcatc atcattggaa agagcaacag catcacctta agattgaatc ctgttgccgg
     8041 tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat
     8101 gtaatgcata gcttgagtat tctatagtgt cacctaaata gcttggcgta atcatggtca
     8161 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     8221 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     8281 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     8341 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     8401 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     8461 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     8521 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     8581 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     8641 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     8701 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     8761 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     8821 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     8881 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     8941 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     9001 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     9061 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     9121 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     9181 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     9241 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     9301 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     9361 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     9421 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     9481 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     9541 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     9601 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     9661 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     9721 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     9781 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     9841 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     9901 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     9961 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    10021 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    10081 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    10141 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    10201 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
    10261 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga tgcggtgtga
    10321 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaag cgttaatatt
    10381 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
    10441 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
    10501 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
    10561 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
    10621 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
    10681 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
    10741 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
    10801 ccgctacagg gcgcgtccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
    10861 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
    10921 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattgt
    10981 aatacgactc actata
//
