LOCUS Exported 10560 bp ds-DNA circular SYN 19-FEB-2017 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pNOC-ARS-CRISPR-P2A-BlastR SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10560) AUTHORS . TITLE Direct Submission JOURNAL Exported Friday, Jul 14, 2017 from SnapGene Viewer 3.3.4 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10560 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 22..126 /gene="bla" /label=AmpR promoter CDS 127..987 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 1158..1746 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" terminator 2223..2822 /label=LDSP terminator CDS complement(2860..3246) /codon_start=1 /label=BlastR /translation="MPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGVN VYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIKA IVKDSDGQPTAVGIRELLPSGYVWE" CDS complement(3247..3426) /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="FCPRPGIHDPAAFSKSHPENSDGRSERLSTAMTTLSFQGPGATNF SLLKQAGDVEENPGP" CDS complement(3436..3456) /codon_start=1 /product="nuclear localization signal of SV40 large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS complement(3472..3498) /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" CDS complement(3499..4014) /codon_start=1 /label=Nlux /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW RLCERILAG" CDS complement(4039..8151) /codon_start=1 /label=Cas9 /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD ATLIHQSITGLYETRIDLSQLGGDSRAD" CDS complement(8152..8172) /codon_start=1 /product="nuclear localization signal of SV40 large T antigen" /label=SV40 NLS /translation="PKKKRKV" promoter 8183..9366 /label=Ribi misc_feature 9395..9474 /label=sgRNA misc_feature 9475..9542 /label=HDV terminator 9554..10527 /label=CS terminator ORIGIN 1 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca 61 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg 121 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc 181 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg 241 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt 301 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta 361 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat 421 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga 481 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca 541 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact 601 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc 661 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact 721 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt 781 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt 841 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt 901 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata 961 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag 1021 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat 1081 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa 1141 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca 1201 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt 1261 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg 1321 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc 1381 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga 1441 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc 1501 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc 1561 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca 1621 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg 1681 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta 1741 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct 1801 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag 1861 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa 1921 gctcaggtca ctggattttg gttttaggaa ttagaaattt tattgataga agtattttac 1981 aaatacaaat acatactaag ggtttcttat atgctcaaca catgagcgaa accctataag 2041 aaccctaatt cccttatctg ggaactactc acacattatt ctggagaaaa atagagagag 2101 atagatttgt agagagagac tggtgatttt tgcggactcc ggtcggcatc tactactagt 2161 gcggccgcca tggcctctag tggatcagct tgcatgcctg caggagacga tatcacctct 2221 tctgtttcca cgataaaaat agactgctca tttcttcgtc gtcttcatcg tctgcttttt 2281 ctgcttcgcc tctgtctggg gtctgaaacc actacacaca cacacaacac tcgtactccc 2341 actttcacaa aagcgtaagc tcaccggctt ttcttacacg tacattttag tggatcccat 2401 cacgccacta ccacgcccgc gggggatgga acggagggga gagagagaag ggggaagcat 2461 ggatgaatga gacattgagg gaaaggaggg gagggagcag tccatcaggg cgctacctct 2521 cttgtccccc aaaccctgtt gagccgttca acatgtttca tgtttcctcc ctcccccctt 2581 ccctccctgg cctttccgcg gagccattca agtgacgtct ggaccgcacc gtaacaaaat 2641 cgtttctatg gggggtttgt ttgacaacca cgtcttcagc gtttttaaaa aaaaaagcgg 2701 gcaagccctc tcaccctcac tcatgcccat cctcctcctc tcctgcggaa cattcttaca 2761 aaaggcgtaa ctcgacgaca actcaaagaa cgacaaacat caatcccaaa aaaaaaatct 2821 ctactcgtct ctcttggatc tttccaattg actagttcac tcccacacgt agcccgaagg 2881 gagaagttct ctgataccga ccgccgtagg ctgcccgtcg gaatccttca caatcgcctt 2941 gatcccaggg tggaggtcga gcaggacctg ccggcaccgc ccgcaggggc tcagaatccc 3001 gcggttctcg ttcccgatgg cgacaatgca cgtcaggttc ccagccgcgg cagcagcggc 3061 ggtccccagg accaccagct ccgcgcaagg cccccccgta aaatggtaca cgttgacccc 3121 cgtgaagatc cgtccgtcgg acgacagagc agcggaagcc accgagtaat cctcggagat 3181 aggaatcgag ttaatcgtcg ccgtagccct ctcgatcagc gtgctctcct cctgcgagag 3241 gggcatgggc cccgggttct cctccacgtc cccggcctgc ttcagcaagg agaagttcgt 3301 cgcccccggc ccctggaagg acagggtcgt catcgcggtg ctcaggcgtt cgctgcggcc 3361 atcgctgttt tccggatggc ttttgctaaa cgccgccgga tcatgaatgc cggggcgcgg 3421 gcaaaagccg cttccgacct tcctcttctt tttcgggcta gccaccatgg acgcgtagtc 3481 gggcacgtcg taggggtatc cggccaggat gcgctcgcac aggcgccagc cggttacccc 3541 gttgatggtc acgcggaaca acagcgagcc atccgggttg atcagccgct cgtcaatgat 3601 cttattgccg ttccacaggg tgccggtcac cgtgatcttc ttgccgtcga acacggcaat 3661 gccctcgtaa ggacggccga agtagtcaat catgttcggc gtcacaccgt cgatgaccag 3721 cgtcccgtag tgcagtatca ccttgaagtg gtgatcgtcc acggggtaca caaccttgaa 3781 gatcttctcg atctggccca tttggtcccc gctcaggccc tcgtagggaa tgatgacgtg 3841 gatgtcgatc ttcaggccgt tctcgccgct caggacgatg cgctggattg gggtcacgga 3901 gacgcccagg ttctggaaaa gcgaggacac accgccctgc tccagcacct ggtcgaggtt 3961 atagccagct gtctgccgcc agtcgcccac gaagtcctcg agagtaaaca ccatgttaac 4021 ggacccgctg ccggacccgt cagccctgct gtctccaccg agctgagaga ggtcgattct 4081 tgtttcatag agccccgtaa ttgactgatg aatcagtgtg gcgtccagga cctcctttgt 4141 agaggtgtac cgctttctgt ctatggtggt gtcgaagtac ttgaaggctg caggcgcgcc 4201 caagttggtc agagtaaaca agtggataat gttttctgcc tgctccctga tgggcttatc 4261 cctgtgctta ttgtaagcag aaagcacctt atcgaggtta gcgtcggcga ggatcactct 4321 tttggagaat tcgcttattt gctcgatgat ctcatcaagg tagtgtttgt gttgttccac 4381 gaacagctgc ttctgctcat tatcttcggg agaccctttg agcttttcat agtggctggc 4441 cagatacaag aaattaacgt atttagaggg cagtgccagc tcgttacctt tctgcagctc 4501 gcccgcacta gcgagcattc gtttccggcc gttttcaagc tcaaagagag agtacttggg 4561 aagcttaatg atgaggtctt ttttgacctc tttatatcct ttcgcctcga gaaagtcgat 4621 ggggtttttt tcgaagcttg atcgctccat gattgtgatg cccagcagtt ccttgacgct 4681 tttgagtttt ttagacttcc ctttctccac tttggccaca accagtacac tgtaagcgac 4741 tgtaggagaa tcgaatccgc cgtatttctt ggggtcccaa tcttttttgc gtgcgatcag 4801 cttgtcgctg ttccttttcg ggaggatact ttccttggag aagcctccgg tctgtacttc 4861 ggtcttttta acgatgttca cctgcggcat ggacaggacc ttccggactg tcgcgaaatc 4921 cctacccttg tcccacacga tttctcctgt ttctccgttt gtttcgataa gtggtcgctt 4981 ccgaatctct ccattggcca gtgtaatctc ggtcttgaaa aaattcataa tattgctgta 5041 aaagaagtac ttagcggtgg ccttgcctat ttcctgctca gactttgcga tcattttcct 5101 aacatcgtac actttatagt ctccgtaaac aaattcagat tcaagcttgg gatatttttt 5161 gataagtgca gtgcctacca ctgcattcag gtaggcatca tgcgcatggt ggtaattgtt 5221 gatctctctc accttataaa actgaaagtc ctttctgaaa tctgagacca gcttagactt 5281 cagagtaata actttcacct ctcgaatcag tttgtcattt tcatcgtact tggtgttcat 5341 gcgtgaatcg agaatttggg ccacgtgctt ggtgatctgg cgtgtctcaa caagctgcct 5401 tttgatgaag ccggctttat ccaactcaga caggccacct cgttcagcct tagtcagatt 5461 atcgaacttc cgttgtgtga tcagtttggc gttcagcagc tgccgccaat aatttttcat 5521 tttcttgaca acttcttctg aggggacgtt atcactcttc cctctatttt tatcggatct 5581 tgtcaacact ttattatcaa tagaatcatc tttgagaaaa gactggggca cgatatgatc 5641 cacgtcgtag tcggagagcc gattgatgtc cagttcctga tccacgtaca tgtccctgcc 5701 gttctgcagg tagtacaggt agagcttctc attctgaagc tgggtgtttt caactgggtg 5761 ttccttaagg atttgggacc ccagttcttt tataccctct tcaatcctct tcatcctttc 5821 cctactgttc ttctgtccct tctgggtagt ttggttctct cgggccatct cgataacgat 5881 attctcgggc ttatgccttc ccattacttt gacgagttca tccacgacct taacggtctg 5941 cagtattccc tttttgatag ctgggctacc tgcaagatta gcgatgtgct cgtgaagact 6001 gtccccctgg ccagaaactt gtgctttctg gatgtcctcc ttaaaggtga gagagtcatc 6061 atggatcaac tgcatgaagt tccggttggc aaatccatcg gacttaagaa aatccaggat 6121 tgtctttcca ctctgcttgt ctcggatccc attgatcagt tttcttgaca gccgccccca 6181 tcctgtatat cggcgcctct tgagctgttt catgactttg tcgtcgaaga gatgagcgta 6241 agttttcaag cgttcttcaa tcatctccct atcttcaaac aacgtaaggg tgaggacaat 6301 gtcctcaaga atgtcctcgt tctcctcatt gtccaggaag tccttgtctt taatgatttt 6361 caggagatcg tgatacgttc ccagggatgc gttgaagcga tcctccactc cgctgatttc 6421 aacagagtcg aaacattcaa tctttttgaa atagtcttct ttgagctgtt tcacggtaac 6481 tttccggttc gtcttgaaga ggaggtccac gatagctttc ttctgctctc cagacaggaa 6541 tgctggcttt ctcatccctt ctgtgacgta tttgaccttg gtgagctcgt tataaactgt 6601 gaagtactcg tacagcagag agtgtttagg aagcaccttt tcgttaggca gatttttatc 6661 aaagttagtc atcctttcga tgaaggactg ggcagaggcc cccttatcca cgacttcctc 6721 gaagttccag ggagtgatgg tctcttctga tttgcgagtc atccacgcga atctggaatt 6781 tccccgggcg agggggccta catagtaggg tatccgaaat gtgaggattt tctcaatctt 6841 ttccctgtta tctttcaaaa aggggtagaa atcctcttgc cgcctgagga tagcgtgcag 6901 ttcgcccagg tgaatctggt gggggatgct tccattgtcg aaagtgcgct gtttgcgcaa 6961 cagatcttct ctgttaagct ttaccagcag ctcctcggtg ccgtccattt tttccaagat 7021 gggcttaata aatttgtaaa attcctcctg gcttgctccg ccgtcaatgt atccggcgta 7081 gccattttta gactgatcga agaaaatttc cttgtacttc tcaggcagtt gctgtctgac 7141 aagggccttc agcaaagtca agtcttggtg gtgctcatca tagcgcttga tcatactagc 7201 gctcagcgga gctttggtga tctccgtgtt cactcgcaga atatcactca gcagaatggc 7261 gtctgacagg ttctttgccg ccaaaaaaag gtctgcgtac tggtcgccga tctgggccag 7321 cagattgtcg agatcatcat cgtaggtgtc tttgctcagt tgaagcttgg catcttcggc 7381 caggtcgaag ttagatttaa agttgggggt cagcccgagt gacagggcga taagattacc 7441 aaacaggccg ttcttcttct ccccagggag ctgtgcgatg aggttttcga gccgccggga 7501 tttggacagc ctagcgctca ggattgcttt ggcgtcaact ccggatgcgt tgatcgggtt 7561 ctcttcgaaa agctgattgt aagtctgaac cagttggata aagagtttgt cgacatcgct 7621 gttgtctggg ttcaggtccc cctcgatgag gaagtgtccc cgaaatttga tcatatgcgc 7681 cagcgcgaga tagatcaacc gcaagtcagc cttatcagta ctgtctacaa gcttcttcct 7741 cagatgatat atggttgggt acttttcatg gtacgccacc tcgtccacga tattgccaaa 7801 gattgggtgg cgctcgtgct ttttatcctc ctccaccaaa aaggactcct ccagcctatg 7861 gaagaaagag tcatccacct tagccatctc attactaaag atctcctgca ggtagcagat 7921 ccgattcttt ctgcgggtat atctgcgccg tgctgttctt ttgagccgcg tggcttcggc 7981 cgtctccccg gagtcgaaca ggagggcgcc aatgaggttc ttctttatgc tgtggcgatc 8041 ggtattgccc agaactttga attttttgct cggcaccttg tactcgtccg taatgacggc 8101 ccagccgacg ctgtttgtgc cgatatcgag cccaatggag tacttcttgt ccaccttccg 8161 ctttttcttg ggcatgctcg agggttgcgt gtgtatctgt gtgcagtgga tattgttacc 8221 gagtttggtg agcgtgagtc cgttagtgcc ctggtggtgg tggattagga gagtgggtga 8281 ctcggtgtcc atggctttct tcgctcatta taggagggga aaggaatgag ggagggtggg 8341 gagaccgcgt ctgttgttga ccaccgattt acttcttgcc tcccttcccc tccctcccct 8401 caatccgtac gacacaaata gtagccgagt gtctgctgca gagcgcatga ttagtgtggt 8461 agacaacgag ggagggaagg atgtacaggg catggcacgg agaagcgatg gtggccagga 8521 agaggagagg tcgcgagaac aggatgtgtt gcgaatggat aaaaacagaa agcgatggct 8581 ctgggcttcg aaagcagggg acattaggac gtgtagacca tctcgacgga tccctctgta 8641 tctctgttgt gcgtgaatgt tttctgtgca cgtgtagtgt gtgagagtag aacccgggaa 8701 ctcgaacaga gaaaagcatg ggtggctgtg gtgtggaggc ttcgttccca ccacatgccc 8761 ttctccttcg cctcgcctct ccctgccttc ttccacgcac ccttgcgccc ctcgttctca 8821 atacctggct cacttccacc attcaaacaa ccatcacgat acaggcattt atctatcgtt 8881 gaagacttct tcctccggta gatcttagcc aaggtaagaa gaggggcatg cagcaaggag 8941 aaagaaatga tgcatgatga ggaatagaag gggaggaggg agggatatga tgggaagcga 9001 aagcgcatat tctggtggtc tgctgcctga tggggacgcg tctagctgtg acactgagga 9061 cggtggctgc tggtggctgc gggcgctgcc ttggtgatca atgggagtaa agggagggaa 9121 ggaggtagcg tgaacggatg acgcggagaa gtttaggggt ctctttacgt atcgcccctg 9181 ccgcccgcct ctctgcgata aatgtgcctg ttaccctgca gcctctattc ttcactgtgt 9241 tcctgttttc caacagcctc tattcttccc tgtcttttgt tgcagtggcg tcatcctctc 9301 tttgccccag tcgtcgttct ctcgactcac tcactccccc cctccttccc tccctccatc 9361 cacagaatcg atgaagagca ggccttggct cttcgtttta gagctagaaa tagcaagtta 9421 aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc ttttggccgg 9481 catggtccca gcctcctcgc tggcgccggc tgggcaacat gcttcggcat ggcgaatggg 9541 actcctgggt accatgggaa agaaaggatg agaaaggaga aaggacatct agataaccgg 9601 catatcacgg tggtgtatta gtgtagaata gtgaagagaa gacttgggaa aatgtgtagg 9661 aaaggttgtt tctgtgtatg tgggttggga tgggtggctg tttgagaagg aacagcgggc 9721 agggcgatgt agtgctgaac gggcacggaa ccactgagac tgaaggaagt agggagagag 9781 aggggcaggg gacgtgcact ttaatctttg cctcggtaga gtatacccat gcaagagtat 9841 gtggccacct gtggtggctt ttggccaggt ctggtgcagt gccaatcatc tcccatcaat 9901 aatacaactt cagaacaacg gcgcattgat ggggagagag aaagtaaatt taagtaaggg 9961 gtacgtagta gaggattcaa ctgaaatatt ttcgaggagc ggttgggaag ttgaccgatt 10021 gaaaggagaa gggaggggag caggtgtgat agtcatgtgt aaagtaattc ttttttgccg 10081 tcgtcacaca atccacatca atgataaaat atgtttaagg atcaatcaca cggagtcggt 10141 cataaggcaa ccgcaaacgc aatgcaaact agcaagcaag caggaccaca acaacaacca 10201 ttaccatcac aggcgacagc agcagcagct gcagcagcag caaggcaagc aaaggccatc 10261 tgcgcgtgga cttttccagg caccggcttt aagcgtaatt ttcaatgatt gttgtccgtg 10321 tatcctctca ccctttcacc gcttgcgcgc acgtgagcag cacaactggt aatgcccgag 10381 gacaatagac gaggaaaaag aagaagaaaa atgaagaggg agtgatgaag aagaagagaa 10441 gaataaaaga agacagaaaa gaagataaaa aagtcaaaag acgacaaaga cggaaaaaaa 10501 agaaacgcgc aattttaaca ccagcaacga cgacgaaaag ggcgtcagct ttgcaccggt //