LOCUS       Exported               18804 bp ds-DNA     circular SYN 14-APR-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pH-dCas9-PBE
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, May 30, 2017 from SnapGene 3.3.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..18804
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    21..45
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     248..264
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        331..710
                     /label=OsU3 promoter
                     /note="Oryza sativa (rice) snRNA U3 promoter"
     CDS             990..1781
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_RNA        1933..2008
                     /label=g RNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        2312..4303
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             4314..4334
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    4335..5015
                     /label=rAPOBEC1
     misc_feature    5016..5063
                     /label=XTEN
     misc_feature    5070..9218
                     /label=dCas9
     CDS             5070..9170
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    9240..9500
                     /label=UGI
     CDS             9501..9521
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(10179..10195)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    10203..10219
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(10227..10257)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10272..10293
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10484..11161
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             11229..12254
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    12294..12468
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    12546..12570
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             12995..13789
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      13876..14464
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    14650..14790
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      15134..15328
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(15394..16467)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(16896..17525)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta
       61 aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc
      121 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      181 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      241 cgacgttgta aaacgacggc cagtgccaag cttagtaatt catccaggtc accaagttct
      301 aggattttca gaactgcaac ttattttatc aaggaatctt taaacatacg aacagatcac
      361 ttaaagttct tctgaagcaa cttaaagtta tcaggcatgc atggatcttg gaggaatcag
      421 atgtgcagtc agggaccata gcacaagaca ggcgtcttct actggtgcta ccagcaaatg
      481 ctggaagccg ggaacactgg gtacgttgga aaccacgtga tgtgaagaag taagataaac
      541 tgtaggagaa aagcatttcg tagtgggcca tgaagccttt caggacatgt attgcagtat
      601 gggccggccc attacgcaat tggacgacaa caaagactag tattagtacc acctcggcta
      661 tccacataga tcaaagctga tttaaaagag ttgtgcagat gatccgtggc gtgagaccaa
      721 cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac
      781 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca
      841 tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag
      901 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg
      961 gcagtcgccc taaaacaaag ttaaacatca tgggggaagc ggtgatcgcc gaagtatcga
     1021 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
     1081 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc
     1141 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
     1201 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
     1261 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
     1321 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
     1381 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
     1441 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
     1501 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
     1561 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact
     1621 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt
     1681 atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc
     1741 actacgtgaa aggcgagatc accaaggtag tcggcaaata atgtctagct agaaattcgt
     1801 tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac taagcacata
     1861 attgctcaca gccaaactat caggtcaagt ctgcttttat tatttttaag cgtgcataat
     1921 aagccggtct cggttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca
     1981 acttgaaaaa gtggcaccga gtcggtgctt ttttttttcg ttttgcattg agttttctcc
     2041 gtcgcatgtt tgcagtttta ttttccgttt tgcattgaaa tttctccgtc tcatgtttgc
     2101 agcgtgttca aaaagtacgc agctgtattt cacttattta cggcgccaca ttttcatgcc
     2161 gtttgtgcca actatcccga gctagtgaat acagcttggc ttcacacaac actggtgacc
     2221 cgctgacctg ctcgtacctc gtaccgtcgt acggcacagc atttggaatt aaagggtgtg
     2281 atcgatactg cttgctgcta agcttgcatg cctgcagtgc agcgtgaccc ggtcgtgccc
     2341 ctctctagag ataatgagca ttgcatgtct aagttataaa aaattaccac atattttttt
     2401 tgtcacactt gtttgaagtg cagtttatct atctttatac atatatttaa actttactct
     2461 acgaataata taatctatag tactacaata atatcagtgt tttagagaat catataaatg
     2521 aacagttaga catggtctaa aggacaattg agtattttga caacaggact ctacagtttt
     2581 atctttttag tgtgcatgtg ttctcctttt tttttgcaaa tagcttcacc tatataatac
     2641 ttcatccatt ttattagtac atccatttag ggtttagggt taatggtttt tatagactaa
     2701 tttttttagt acatctattt tattctattt tagcctctaa attaagaaaa ctaaaactct
     2761 attttagttt ttttatttaa taatttagat ataaaataga ataaaataaa gtgactaaaa
     2821 attaaacaaa taccctttaa gaaattaaaa aaactaagga aacatttttc ttgtttcgag
     2881 tagataatgc cagcctgtta aacgccgtcg acgagtctaa cggacaccaa ccagcgaacc
     2941 agcagcgtcg cgtcgggcca agcgaagcag acggcacggc atctctgtcg ctgcctctgg
     3001 acccctctcg agagttccgc tccaccgttg gacttgctcc gctgtcggca tccagaaatt
     3061 gcgtggcgga gcggcagacg tgagccggca cggcaggcgg cctcctcctc ctctcacggc
     3121 acggcagcta cgggggattc ctttcccacc gctccttcgc tttcccttcc tcgcccgccg
     3181 taataaatag acaccccctc cacaccctct ttccccaacc tcgtgttgtt cggagcgcac
     3241 acacacacaa ccagatctcc cccaaatcca cccgtcggca cctccgcttc aaggtacgcc
     3301 gctcgtcctc cccccccccc cctctctacc ttctctagat cggcgttccg gtccatggtt
     3361 agggcccggt agttctactt ctgttcatgt ttgtgttaga tccgtgtttg tgttagatcc
     3421 gtgctgctag cgttcgtaca cggatgcgac ctgtacgtca gacacgttct gattgctaac
     3481 ttgccagtgt ttctctttgg ggaatcctgg gatggctcta gccgttccgc agacgggatc
     3541 gatttcatga ttttttttgt ttcgttgcat agggtttggt ttgccctttt cctttatttc
     3601 aatatatgcc gtgcacttgt ttgtcgggtc atcttttcat gctttttttt gtcttggttg
     3661 tgatgatgtg gtctggttgg gcggtcgttc tagatcggag tagaattctg tttcaaacta
     3721 cctggtggat ttattaattt tggatctgta tgtgtgtgcc atacatattc atagttacga
     3781 attgaagatg atggatggaa atatcgatct aggataggta tacatgttga tgcgggtttt
     3841 actgatgcat atacagagat gctttttgtt cgcttggttg tgatgatgtg gtgtggttgg
     3901 gcggtcgttc attcgttcta gatcggagta gaatactgtt tcaaactacc tggtgtattt
     3961 attaattttg gaactgtatg tgtgtgtcat acatcttcat agttacgagt ttaagatgga
     4021 tggaaatatc gatctaggat aggtatacat gttgatgtgg gttttactga tgcatataca
     4081 tgatggcata tgcagcatct attcatatgc tctaaccttg agtacctatc tattataata
     4141 aacaagtatg ttttataatt attttgatct tgatatactt ggatgatggc atatgcagca
     4201 gctatatgtg gattttttta gccctgcctt catacgctat ttatttgctt ggtactgttt
     4261 cttttgtcga tgctcaccct gttgtttggt gttacttctg cagccctagg atgccaaaga
     4321 agaagaggaa ggtttcatcg gagaccggcc ctgttgctgt tgaccccacc ctgcggcgga
     4381 gaatcgagcc acacgagttc gaggtgttct tcgacccaag ggagctccgc aaggagacgt
     4441 gcctcctgta cgagatcaac tggggcggca ggcactccat ctggaggcac accagccaaa
     4501 acaccaacaa gcacgtggag gtcaacttca tcgagaagtt caccaccgag aggtacttct
     4561 gcccaaacac ccgctgctcc atcacctggt tcctgtcctg gagcccatgc ggcgagtgct
     4621 ccagggccat caccgagttc ctcagccgct acccacacgt caccctgttc atctacatcg
     4681 ccaggctcta ccaccacgcc gacccaagga acaggcaggg cctccgcgac ctgatctcca
     4741 gcggcgtgac catccaaatc atgaccgagc aggagtccgg ctactgctgg aggaacttcg
     4801 tcaactactc cccaagcaac gaggcccact ggccaaggta cccacacctc tgggtgcgcc
     4861 tctacgtgct cgagctgtac tgcatcatcc tcggcctgcc accatgcctc aacatcctga
     4921 ggcgcaagca accacagctg accttcttca ccatcgccct ccaaagctgc cactaccaga
     4981 ggctcccacc acacatcctg tgggctaccg gcctcaagtc cggcagcgag acgccaggca
     5041 cctccgagag cgctacgcct gaacttaagg acaagaagta ctcgatcggc ctcgccatcg
     5101 ggacgaactc agttggctgg gccgtgatca ccgacgagta caaggtgccc tctaagaagt
     5161 tcaaggtcct ggggaacacc gaccgccatt ccatcaagaa gaacctcatc ggcgctctcc
     5221 tgttcgacag cggggagacc gctgaggcta cgaggctcaa gagaaccgct aggcgccggt
     5281 acacgagaag gaagaacagg atctgctacc tccaagagat tttctccaac gagatggcca
     5341 aggttgacga ttcattcttc caccgcctgg aggagtcttt cctcgtggag gaggataaga
     5401 agcacgagcg gcatcccatc ttcggcaaca tcgtggacga ggttgcctac cacgagaagt
     5461 accctacgat ctaccatctg cggaagaagc tcgtggactc caccgataag gcggacctca
     5521 gactgatcta cctcgctctg gcccacatga tcaagttccg cggccatttc ctgatcgagg
     5581 gggatctcaa cccagacaac agcgatgttg acaagctgtt catccaactc gtgcagacct
     5641 acaaccaact cttcgaggag aacccgatca acgcctctgg cgtggacgcg aaggctatcc
     5701 tgtccgcgag gctctcgaag tccaggaggc tggagaacct gatcgctcag ctcccaggcg
     5761 agaagaagaa cggcctgttc gggaacctca tcgctctcag cctggggctc accccgaact
     5821 tcaagtcgaa cttcgatctc gctgaggacg ccaagctgca actctccaag gacacctacg
     5881 acgatgacct cgataacctc ctggcccaga tcggcgatca atacgcggac ctgttcctcg
     5941 ctgccaagaa cctgtcggac gccatcctcc tgtcagatat cctccgcgtg aacaccgaga
     6001 tcacgaaggc tccactctct gcctccatga tcaagcgcta cgacgagcac catcaggatc
     6061 tgaccctcct gaaggcgctg gtccgccaac agctcccgga gaagtacaag gagattttct
     6121 tcgatcagtc gaagaacggc tacgctgggt acatcgacgg cggggcctca caagaggagt
     6181 tctacaagtt catcaagcca atcctggaga agatggacgg cacggaggag ctcctggtga
     6241 agctcaacag ggaggacctc ctgcggaagc agagaacctt cgataacggc agcatccccc
     6301 accaaatcca tctcggggag ctgcacgcca tcctgagaag gcaagaggac ttctaccctt
     6361 tcctcaagga taaccgggag aagatcgaga agatcctgac cttcagaatc ccatactacg
     6421 tcggccctct cgcgcggggg aactcaagat tcgcttggat gacccgcaag tctgaggaga
     6481 ccatcacgcc gtggaacttc gaggaggtgg tggacaaggg cgctagcgct cagtcgttca
     6541 tcgagaggat gaccaacttc gacaagaacc tgcccaacga gaaggtgctc cctaagcact
     6601 cgctcctgta cgagtacttc accgtctaca acgagctcac gaaggtgaag tacgtcaccg
     6661 agggcatgcg caagccagcg ttcctgtccg gggagcagaa gaaggctatc gtggacctcc
     6721 tgttcaagac caaccggaag gtcacggtta agcaactcaa ggaggactac ttcaagaaga
     6781 tcgagtgctt cgattcggtc gagatcagcg gcgttgagga ccgcttcaac gccagcctcg
     6841 ggacctacca cgatctcctg aagatcatca aggataagga cttcctggac aacgaggaga
     6901 acgaggatat cctggaggac atcgtgctga ccctcacgct gttcgaggac agggagatga
     6961 tcgaggagcg cctgaagacg tacgcccatc tcttcgatga caaggtcatg aagcaactca
     7021 agcgccggag atacaccggc tgggggaggc tgtcccgcaa gctcatcaac ggcatccggg
     7081 acaagcagtc cgggaagacc atcctcgact tcctcaagag cgatggcttc gccaacagga
     7141 acttcatgca actgatccac gatgacagcc tcaccttcaa ggaggatatc caaaaggctc
     7201 aagtgagcgg ccagggggac tcgctgcacg agcatatcgc gaacctcgct ggctcccccg
     7261 cgatcaagaa gggcatcctc cagaccgtga aggttgtgga cgagctcgtg aaggtcatgg
     7321 gccggcacaa gcctgagaac atcgtcatcg agatggccag agagaaccaa accacgcaga
     7381 aggggcaaaa gaactctagg gagcgcatga agcgcatcga ggagggcatc aaggagctgg
     7441 ggtcccaaat cctcaaggag cacccagtgg agaacaccca actgcagaac gagaagctct
     7501 acctgtacta cctccagaac ggcagggata tgtacgtgga ccaagagctg gatatcaacc
     7561 gcctcagcga ttacgacgtc gatgctatcg ttccccagtc tttcctgaag gatgactcca
     7621 tcgacaacaa ggtcctcacc aggtcggaca agaaccgcgg caagtcagat aacgttccat
     7681 ctgaggaggt cgttaagaag atgaagaact actggaggca gctcctgaac gccaagctga
     7741 tcacgcaaag gaagttcgac aacctcacca aggctgagag aggcgggctc tcagagctgg
     7801 acaaggccgg cttcatcaag cggcagctgg tcgagaccag acaaatcacg aagcacgttg
     7861 cgcaaatcct cgactctcgg atgaacacga agtacgatga gaacgacaag ctgatcaggg
     7921 aggttaaggt gatcaccctg aagtctaagc tcgtctccga cttcaggaag gatttccagt
     7981 tctacaaggt tcgcgagatc aacaactacc accatgccca tgacgcttac ctcaacgctg
     8041 tggtcggcac cgctctgatc aagaagtacc caaagctgga gtccgagttc gtgtacgggg
     8101 actacaaggt ttacgatgtg cgcaagatga tcgccaagtc ggagcaagag atcggcaagg
     8161 ctaccgccaa gtacttcttc tactcaaaca tcatgaactt cttcaagacc gagatcacgc
     8221 tggccaacgg cgagatccgg aagagaccgc tcatcgagac caacggcgag acgggggaga
     8281 tcgtgtggga caagggcagg gatttcgcga ccgtccgcaa ggttctctcc atgccccagg
     8341 tgaacatcgt caagaagacc gaggtccaaa cgggcgggtt ctcaaaggag tctatcctgc
     8401 ctaagcggaa cagcgacaag ctcatcgcca gaaagaagga ctgggaccca aagaagtacg
     8461 gcgggttcga cagccctacc gtggcctact cggtcctggt tgtggcgaag gttgagaagg
     8521 gcaagtccaa gaagctcaag agcgtgaagg agctcctggg gatcaccatc atggagaggt
     8581 ccagcttcga gaagaaccca atcgacttcc tggaggccaa gggctacaag gaggtgaaga
     8641 aggacctgat catcaagctc ccgaagtact ctctcttcga gctggagaac ggcaggaaga
     8701 gaatgctggc ttccgctggc gagctccaga aggggaacga gctcgcgctg ccaagcaagt
     8761 acgtgaactt cctctacctg gcttcccact acgagaagct caagggcagc ccggaggaca
     8821 acgagcaaaa gcagctgttc gtcgagcagc acaagcatta cctcgacgag atcatcgagc
     8881 aaatctccga gttcagcaag cgcgtgatcc tcgccgacgc gaacctggat aaggtcctct
     8941 ccgcctacaa caagcaccgg gacaagccca tcagagagca agcggagaac atcatccatc
     9001 tcttcaccct gacgaacctc ggcgctcctg ctgctttcaa gtacttcgac accacgatcg
     9061 atcggaagag atacacctcc acgaaggagg tcctggacgc gaccctcatc caccagtcga
     9121 tcaccggcct gtacgagacg aggatcgacc tctcacaact cggcggggat aagagacccg
     9181 cagcaaccaa gaaggcaggg caagcaaaga agaagaagac gcgtgactcc ggcggcagca
     9241 ccaacctgtc cgacatcatc gagaaggaga cgggcaagca actcgtgatc caggagagca
     9301 tcctcatgct gccagaggag gtggaggagg tcatcggcaa caagccagag tccgacatcc
     9361 tggtgcacac cgcctacgac gagtccaccg acgagaacgt catgctcctg accagcgacg
     9421 ccccagagta caagccatgg gccctcgtca tccaggacag caacggggag aacaagatca
     9481 agatgctgtc gggggggagc ccaaagaaga agcggaaggt gtagagctca gagctttcgt
     9541 tcgtatcatc ggtttcgaca acgttcgtca agttcaatgc atcagtttca ttgcgcacac
     9601 accagaatcc tactgagttt gagtattatg gcattgggaa aactgttttt cttgtaccat
     9661 ttgttgtgct tgtaatttac tgtgtttttt attcggtttt cgctatcgaa ctgtgaaatg
     9721 gaaatggatg gagaagagtt aatgaatgat atggtccttt tgttcattct caaattaata
     9781 ttatttgttt tttctcttat ttgttgtgtg ttgaatttga aattataaga gatatgcaaa
     9841 cattttgttt tgagtaaaaa tgtgtcaaat cgtggcctct aatgaccgaa gttaatatga
     9901 ggagtaaaac acttgtagtt gtaccattat gcttattcac taggcaacaa atatattttc
     9961 agacctagaa aagctgcaaa tgttactgaa tacaagtatg tcctcttgtg ttttagacat
    10021 ttatgaactt tcctttatgt aattttccag aatccttgtc agattctaat cattgcttta
    10081 taattatagt tatactcatg gatttgtagt tgagtatgaa aatatttttt aatgcatttt
    10141 atgacttgcc aattgattga caacgaattc gtaatcatgt catagctgtt tcctgtgtga
    10201 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
    10261 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
    10321 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
    10381 ggtttgcgta ttggctagag cagcttgcca acatggtgga gcacgacact ctcgtctact
    10441 ccaagaatat caaagataca gtctcagaag accaaagggc tattgagact tttcaacaaa
    10501 gggtaatatc gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcaaaa
    10561 ggacagtaga aaaggaaggt ggcacctaca aatgccatca ttgcgataaa ggaaaggcta
    10621 tcgttcaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca
    10681 tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgataaca
    10741 tggtggagca cgacactctc gtctactcca agaatatcaa agatacagtc tcagaagacc
    10801 aaagggctat tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt
    10861 gcccagctat ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat
    10921 gccatcattg cgataaagga aaggctatcg ttcaagatgc ctctgccgac agtggtccca
    10981 aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt
    11041 caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact
    11101 atccttcgca agaccttcct ctatataagg aagttcattt catttggaga ggacacgctg
    11161 aaatcaccag tctctctcta caaatctatc tctctcgagc tttcgcagat cccggggggc
    11221 aatgagatat gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa
    11281 agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca
    11341 gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct
    11401 acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc
    11461 ttgacattgg ggagtttagc gagagcctga cctattgcat ctcccgccgt gcacagggtg
    11521 tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctacaaccg gtcgcggagg
    11581 ctatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac
    11641 cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc
    11701 atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc
    11761 tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg
    11821 atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga
    11881 gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt
    11941 ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag
    12001 gatcgccacg actccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct
    12061 tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc
    12121 gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga
    12181 ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga
    12241 gggcaaagaa atagagtaga tgccgaccgg atctgtcgat cgacaagctc gagtttctcc
    12301 ataataatgt gtgagtagtt cccagataag ggaattaggg ttcctatagg gtttcgctca
    12361 tgtgttgagc atataagaaa cccttagtat gtatttgtat ttgtaaaata cttctatcaa
    12421 taaaatttct aattcctaaa accaaaatcc agtactaaaa tccagatccc ccgaattaat
    12481 tcggcgttaa ttcagtacat taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc
    12541 aatttgttta caccacaata tatcctgcca ccagccagcc aacagctccc cgaccggcag
    12601 ctcggcacaa aatcaccact cgatacaggc agcccatcag tccgggacgg cgtcagcggg
    12661 agagccgttg taaggcggca gactttgctc atgttaccga tgctattcgg aagaacggca
    12721 actaagctgc cgggtttgaa acacggatga tctcgcggag ggtagcatgt tgattgtaac
    12781 gatgacagag cgttgctgcc tgtgatcacc gcggtttcaa aatcggctcc gtcgatacta
    12841 tgttatacgc caactttgaa aacaactttg aaaaagctgt tttctggtat ttaaggtttt
    12901 agaatgcaag gaacagtgaa ttggagttcg tcttgttata attagcttct tggggtatct
    12961 ttaaatactg tagaaaagag gaaggaaata ataaatggct aaaatgagaa tatcaccgga
    13021 attgaaaaaa ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa tgtctcctgc
    13081 taaggtatat aagctggtgg gagaaaatga aaacctatat ttaaaaatga cggacagccg
    13141 gtataaaggg accacctatg atgtggaacg ggaaaaggac atgatgctat ggctggaagg
    13201 aaagctgcct gttccaaagg tcctgcactt tgaacggcat gatggctgga gcaatctgct
    13261 catgagtgag gccgatggcg tcctttgctc ggaagagtat gaagatgaac aaagccctga
    13321 aaagattatc gagctgtatg cggagtgcat caggctcttt cactccatcg acatatcgga
    13381 ttgtccctat acgaatagct tagacagccg cttagccgaa ttggattact tactgaataa
    13441 cgatctggcc gatgtggatt gcgaaaactg ggaagaagac actccattta aagatccgcg
    13501 cgagctgtat gattttttaa agacggaaaa gcccgaagag gaacttgtct tttcccacgg
    13561 cgacctggga gacagcaaca tctttgtgaa agatggcaaa gtaagtggct ttattgatct
    13621 tgggagaagc ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc ggtcgatcag
    13681 ggaggatatc ggggaagaac agtatgtcga gctatttttt gacttactgg ggatcaagcc
    13741 tgattgggag aaaataaaat attatatttt actggatgaa ttgttttagt acctagaatg
    13801 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
    13861 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
    13921 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
    13981 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
    14041 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
    14101 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
    14161 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
    14221 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
    14281 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
    14341 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
    14401 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
    14461 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
    14521 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
    14581 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
    14641 ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
    14701 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg
    14761 ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg
    14821 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
    14881 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gggtgccttg
    14941 atgtgggcgc cggcggtcga gtggcgacgg cgcggcttgt ccgcgccctg gtagattgcc
    15001 tggccgtagg ccagccattt ttgagcggcc agcggccgcg ataggccgac gcgaagcggc
    15061 ggggcgtagg gagcgcagcg accgaagggt aggcgctttt tgcagctctt cggctgtgcg
    15121 ctggccagac agttatgcac aggccaggcg ggttttaaga gttttaataa gttttaaaga
    15181 gttttaggcg gaaaaatcgc cttttttctc ttttatatca gtcacttaca tgtgtgaccg
    15241 gttcccaatg tacggctttg ggttcccaat gtacgggttc cggttcccaa tgtacggctt
    15301 tgggttccca atgtacgtgc tatccacagg aaacagacct tttcgacctt tttcccctgc
    15361 tagggcaatt tgccctagca tctgctccgt acattaggaa ccggcggatg cttcgccctc
    15421 gatcaggttg cggtagcgca tgactaggat cgggccagcc tgccccgcct cctccttcaa
    15481 atcgtactcc ggcaggtcat ttgacccgat cagcttgcgc acggtgaaac agaacttctt
    15541 gaactctccg gcgctgccac tgcgttcgta gatcgtcttg aacaaccatc tggcttctgc
    15601 cttgcctgcg gcgcggcgtg ccaggcggta gagaaaacgg ccgatgccgg gatcgatcaa
    15661 aaagtaatcg gggtgaaccg tcagcacgtc cgggttcttg ccttctgtga tctcgcggta
    15721 catccaatca gctagctcga tctcgatgta ctccggccgc ccggtttcgc tctttacgat
    15781 cttgtagcgg ctaatcaagg cttcaccctc ggataccgtc accaggcggc cgttcttggc
    15841 cttcttcgta cgctgcatgg caacgtgcgt ggtgtttaac cgaatgcagg tttctaccag
    15901 gtcgtctttc tgctttccgc catcggctcg ccggcagaac ttgagtacgt ccgcaacgtg
    15961 tggacggaac acgcggccgg gcttgtctcc cttcccttcc cggtatcggt tcatggattc
    16021 ggttagatgg gaaaccgcca tcagtaccag gtcgtaatcc cacacactgg ccatgccggc
    16081 cggccctgcg gaaacctcta cgtgcccgtc tggaagctcg tagcggatca cctcgccagc
    16141 tcgtcggtca cgcttcgaca gacggaaaac ggccacgtcc atgatgctgc gactatcgcg
    16201 ggtgcccacg tcatagagca tcggaacgaa aaaatctggt tgctcgtcgc ccttgggcgg
    16261 cttcctaatc gacggcgcac cggctgccgg cggttgccgg gattctttgc ggattcgatc
    16321 agcggccgct tgccacgatt caccggggcg tgcttctgcc tcgatgcgtt gccgctgggc
    16381 ggcctgcgcg gccttcaact tctccaccag gtcatcaccc agcgccgcgc cgatttgtac
    16441 cgggccggat ggtttgcgac cgctcacgcc gattcctcgg gcttgggggt tccagtgcca
    16501 ttgcagggcc ggcagacaac ccagccgctt acgcctggcc aaccgcccgt tcctccacac
    16561 atggggcatt ccacggcgtc ggtgcctggt tgttcttgat tttccatgcc gcctccttta
    16621 gccgctaaaa ttcatctact catttattca tttgctcatt tactctggta gctgcgcgat
    16681 gtattcagat agcagctcgg taatggtctt gccttggcgt accgcgtaca tcttcagctt
    16741 ggtgtgatcc tccgccggca actgaaagtt gacccgcttc atggctggcg tgtctgccag
    16801 gctggccaac gttgcagcct tgctgctgcg tgcgctcgga cggccggcac ttagcgtgtt
    16861 tgtgcttttg ctcattttct ctttacctca ttaactcaaa tgagttttga tttaatttca
    16921 gcggccagcg cctggacctc gcgggcagcg tcgccctcgg gttctgattc aagaacggtt
    16981 gtgccggcgg cggcagtgcc tgggtagctc acgcgctgcg tgatacggga ctcaagaatg
    17041 ggcagctcgt acccggccag cgcctcggca acctcaccgc cgatgcgcgt gcctttgatc
    17101 gcccgcgaca cgacaaaggc cgcttgtagc cttccatccg tgacctcaat gcgctgctta
    17161 accagctcca ccaggtcggc ggtggcccat atgtcgtaag ggcttggctg caccggaatc
    17221 agcacgaagt cggctgcctt gatcgcggac acagccaagt ccgccgcctg gggcgctccg
    17281 tcgatcacta cgaagtcgcg ccggccgatg gccttcacgt cgcggtcaat cgtcgggcgg
    17341 tcgatgccga caacggttag cggttgatct tcccgcacgg ccgcccaatc gcgggcactg
    17401 ccctggggat cggaatcgac taacagaaca tcggccccgg cgagttgcag ggcgcgggct
    17461 agatgggttg cgatggtcgt cttgcctgac ccgcctttct ggttaagtac agcgataacc
    17521 ttcatgcgtt ccccttgcgt atttgtttat ttactcatcg catcatatac gcagcgaccg
    17581 catgacgcaa gctgttttac tcaaatacac atcacctttt tagacggcgg cgctcggttt
    17641 cttcagcggc caagctggcc ggccaggccg ccagcttggc atcagacaaa ccggccagga
    17701 tttcatgcag ccgcacggtt gagacgtgcg cgggcggctc gaacacgtac ccggccgcga
    17761 tcatctccgc ctcgatctct tcggtaatga aaaacggttc gtcctggccg tcctggtgcg
    17821 gtttcatgct tgttcctctt ggcgttcatt ctcggcggcc gccagggcgt cggcctcggt
    17881 caatgcgtcc tcacggaagg caccgcgccg cctggcctcg gtgggcgtca cttcctcgct
    17941 gcgctcaagt gcgcggtaca gggtcgagcg atgcacgcca agcagtgcag ccgcctcttt
    18001 cacggtgcgg ccttcctggt cgatcagctc gcgggcgtgc gcgatctgtg ccggggtgag
    18061 ggtagggcgg gggccaaact tcacgcctcg ggccttggcg gcctcgcgcc cgctccgggt
    18121 gcggtcgatg attagggaac gctcgaactc ggcaatgccg gcgaacacgg tcaacaccat
    18181 gcggccggcc ggcgtggtgg tgtcggccca cggctctgcc aggctacgca ggcccgcgcc
    18241 ggcctcctgg atgcgctcgg caatgtccag taggtcgcgg gtgctgcggg ccaggcggtc
    18301 tagcctggtc actgtcacaa cgtcgccagg gcgtaggtgg tcaagcatcc tggccagctc
    18361 cgggcggtcg cgcctggtgc cggtgatctt ctcggaaaac agcttggtgc agccggccgc
    18421 gtgcagttcg gcccgttggt tggtcaagtc ctggtcgtcg gtgctgacgc gggcatagcc
    18481 cagcaggcca gcggcggcgc tcttgttcat ggcgtaatgt ctccggttct agtcgcaagt
    18541 attctacttt atgcgactaa aacacgcgac aagaaaacgc caggaaaagg gcagggcggc
    18601 agcctgtcgc gtaacttagg acttgtgcga catgtcgttt tcagaagacg gctgcactga
    18661 acgtcagaag ccgactgcac tatagcagcg gaggggttgg atcaaagtac tttgatcccg
    18721 aggggaaccc tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc
    18781 ccttttaaat atccgttatt ctaa
//
