LOCUS       Exported               19582 bp ds-DNA     circular SYN 23-AUG-2017
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pH-nCas9-PBE
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 23, 2017 from SnapGene 4.0.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..19582
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    21..45
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     89..105
                     /label=P2044 hHO1-F
                     /note="Comments: Forward primer to confirm hHO1 insert, 
                     Plasmids: 74669, Source: MGL"
     primer_bind     109..136
                     /label=pUC-3
                     /note="Primer P649, Plasmid 27182, Comments: Confirm 1st 
                     loxP site in pMOD (Forward primer); Wada lab"
     primer_bind     117..132
                     /label=P1941 pRMC2_SEQF
                     /note="Comments: Forward primer to confirm MCS in pRMC2; 
                     Plasmid: pRMC2; Source: JM"
     primer_bind     158..177
                     /label=LacZ-F2
                     /note="Primer P398, Plasmid 22287, Comments: In LacZ gene. 
                     To confirm that 4xHA tag is in-frame with Rev1; AMH"
     primer_bind     202..219
                     /label=SIRT1U-F
                     /note="Primer P171, Plasmid 20379-80, Comments: To confirm 
                     mut in SIRT1 3'UTR; Munekazu Yamakuchi from Lowenstein lab"
     primer_bind     202..219
                     /label=A161.pmirreport
                     /note="Primer P356, Plasmid 15094, Comments: Confirm 
                     strange insertion reported by Chunxing Yang; Yang"
     primer_bind     221..244
                     /label=pBAC
                     /note="P25"
     primer_bind     233..255
                     /label=#44 M13pUC-Fwd
                     /note="In lacZ gene, also called M13 pUC Forward. Created 
                     by: Invitrogen"
     primer_bind     247..264
                     /label=#42 M13-F20
                     /note="In lacZ gene, also called M13 (-21) Forward, M13F, 
                     M13 Forward. Created by: Unknown"
     primer_bind     248..264
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        331..710
                     /label=OsU3 promoter
                     /note="Oryza sativa (rice) snRNA U3 promoter"
     primer_bind     complement(729..748)
                     /label=pFUS_B4-rev
                     /note="Primer p1293, Plasmid 0, Comments: reverse primer 
                     for the Ekker lab TALEN plasmids; mtf"
     primer_bind     complement(729..748)
                     /label=pCR8_R1
                     /note="Primer P693, Plasmid TALEN project, Comments: 
                     Reverse sequencing primer for pCR8; Colby Starker"
     primer_bind     774..787
                     /label=EF1aintseqR
                     /note="Primer P1119, Plasmid 21893, Comments: Confirm 
                     deletion in 5' AAV rep ori ITR as reported by Nick Hand; 
                     Nick Hand"
     primer_bind     complement(954..963)
                     /label=P2159 hSCN2A-F2
                     /note="Comments: Forward primer to confirm mutation in 
                     hSCN2A insert, Plasmids: 84766, Source: AH"
     primer_bind     complement(962..981)
                     /label=Spect-R (pSEVA-R)
                     /note="Primer P1314, Plasmid 45941, 45942, 45943, 45944, 
                     Comments: Reverse plasmid for (some?) pSEVA plasmid 
                     backbones; renamed Spect-R ; Thorben Dammeyer lab (AMJ)"
     CDS             990..1781
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     complement(1195..1214)
                     /label=aadA1-F
                     /note="Primer P110, Plasmid 15456, Comments: pCL25; EP"
     primer_bind     1650..1669
                     /label=Spect-F
                     /note="Primer P1361, Plasmid Deposit 70166, Comments: Fwd 
                     primer for Spectomycin resistance; AMJ"
     misc_RNA        1933..2008
                     /label=g RNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(1979..1999)
                     /label=StrepPyoCas9-5UTR-F
                     /note="Primer P1222, Plasmid 42876, Comments: Anneals in 
                     the 5'UTR of Streptococcus pyogenes Cas9, to seq Cas9 ORF, 
                     fwd dir'n; AMJ"
     primer_bind     1991..2000
                     /label=P1530 hLIMK1-Fwd
                     /note="Comments: To confirm mut around aa270 of human 
                     LIMK1; Plasmid: 23511; Source: LS"
     promoter        2312..4304
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     primer_bind     2669..2679
                     /label=mGata3-R
                     /note="Primer P1164, Plasmid 40568, Comments: Rev primer 
                     from mouse Gata3 out into vector, anneals around bp 435 of 
                     NM_008091.3 ; AMJ"
     primer_bind     complement(2936..2946)
                     /label=Albumin-b
                     /note="Primer P314, Plasmid 22037, Comments: 0; Trono lab"
     primer_bind     complement(3102..3113)
                     /label=mMybbp1a-F
                     /note="Primer P992, Plasmid 41, Comments: verify aa 935 in 
                     Mybbp1a; anneals to 2321-2340 in NM_016776; JN"
     primer_bind     3274..3285
                     /label=P2082 sUra4-F
                     /note="Comments: To confirm ura4 insert, Plasmids: 78404, 
                     Source: AH"
     primer_bind     complement(3598..3615)
                     /label=mYes1-3 (2)
                     /note="P77"
     primer_bind     complement(3598..3615)
                     /label=mYes1-3
                     /note="Primer P77, Plasmid 18068, Comments: 
                     pMIK-Neo-mYes-Y535F; Sequence mouse Yes 1 from 3' end to 
                     get 535 mutation"
     primer_bind     complement(4314..4334)
                     /label=p1958 SV40-NLS-R
                     /note="Comments: no primers anywhere near region to QC 
                     insert; Plasmid: 65838; Source: mac"
     CDS             4315..4335
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    4336..5016
                     /label=rAPOBEC1
     primer_bind     4575..4587
                     /label=hProgerin-F
                     /note="Primer P326, Plasmid 22662, Comments: to get 
                     deletion that results in progerin; MH"
     primer_bind     4619..4629
                     /label=P1879 mBicD2-R
                     /note="Comments: Mouse BicD2 reverse primer to sequence 5' 
                     end and upstream of gene; anneals to bp 516-535 of ref seq 
                     AJ250106; Plasmid: 64205; Source: JN"
     primer_bind     4872..4881
                     /label=P1732 mCherry-F2
                     /note="Comments: Forward primer from the 3' end of mCherry 
                     Exon 2 to verify mir124 Seed Site and mutated Vamp3delta3; 
                     Plasmid: 59891; Source: CLC"
     misc_feature    5017..5064
                     /label=XTEN
     misc_feature    5071..9219
                     /label=nCas9(D10A)
     primer_bind     complement(5392..5411)
                     /label=hSpCas9-R1
                     /note="Primer P1233, Plasmid 42229 & 42333, Comments: 
                     Anneals near the N-terminal end of the humanized S. 
                     pyogenes Crispr-associated Cas9 nuclease, sequencing in the
                     reverse direction to verify 5' NLS and tags; AMJ"
     primer_bind     complement(5612..5627)
                     /label=hGLIS1-Rev
                     /note="Primer P1099, Plasmid 37625, Comments: Rev primer to
                     confirm mutations; anneals approx. aa 246; EJP"
     primer_bind     complement(5847..5856)
                     /label=P2042 ASB11-R
                     /note="Comments: Reverse primer to confirm C-term of H. 
                     sapiens ASB11 insert, Plasmids: 73245, Source: MGL"
     primer_bind     7048..7057
                     /label=#93 RVprimer3
                     /note="From Promega, for pGL3 plasmid; 5' of MCS in pGL3 
                     vector, forward primer; also called RV primer3. Created by:
                     Unknown"
     primer_bind     complement(7127..7146)
                     /label=P1750 CM009 Cas9 M R
                     /note="Comments: Depositor suggested primer to sequence the
                     Cas9 coding insert in this plasmid; Plasmid: 61408; Source:
                     Randy Platt - Feng Zhang lab (AMJ)"
     primer_bind     complement(7365..7384)
                     /label=synthCas9Int-R
                     /note="Primer P1290, Plasmid 46168, Comments: Rev primer to
                     verify site of intron insertion into synthetic 
                     codon-optimized S. pyogenes Cas9 in this plasmid; AMJ"
     primer_bind     7462..7473
                     /label=hEGFR-Fwd2
                     /note="Primer P743, Plasmid deposit 69158, Comments: To 
                     confirm mutations near aa 740; mp"
     primer_bind     7701..7720
                     /label=P1742 SpCas9-F2
                     /note="Comments: Forward primer from Feng Zhang lab SpCas9 
                     (anneals ~895 aa) to verify N963A mutation; Plasmid: 61425;
                     Source: CLC"
     primer_bind     complement(7948..7958)
                     /label=P1647 cMyc-R
                     /note="Comments: Reverse primer to sequence the 5' end of 
                     human cMyc and cMyc-IRES junction; Plasmid: 58972; Source: 
                     CLC"
     primer_bind     complement(8281..8290)
                     /label=P1976 Acly-R
                     /note="Comments: Rev primer to capture relevant 
                     phosho-mutations in middle of inserts; Plasmid: 70768; 
                     Source: MGL"
     primer_bind     complement(8355..8369)
                     /label=hP53-R2
                     /note="Primer P789, Plasmid 32301, Comments: Rev primer 
                     from N-term of human TP53; EJP"
     primer_bind     8404..8416
                     /label=MoCloF
                     /note="Primer P1390, Plasmid Deposit 70459 (MoClo Kit), 
                     Comments: Fwd primer for Marillonnet Mo Clo system vectors;
                     Sylvestre Marillonnet lab (AMJ)"
     primer_bind     complement(8618..8638)
                     /label=P1751 CM010 Cas9 3' R
                     /note="Comments: Depositor suggested primer to sequence the
                     Cas9 coding insert in this plasmid; Plasmid: 61408; Source:
                     Randy Platt - Feng Zhang lab (AMJ)"
     primer_bind     8841..8856
                     /label=hNMHC-F1
                     /note="Primer p980, Plasmid 35689, Comments: confirm stop 
                     codon after aa1928; mp"
     primer_bind     9029..9054
                     /label=P2060 hDNMT3A-F
                     /note="Comments: Confirm flag tag at end of dCas9 and N 
                     term of DNMT3A insert, Plasmids: 78256, 78257, Source: AH"
     primer_bind     9088..9100
                     /label=P1712 dCas9-F2
                     /note="Comments: Forward primer from C-term dCas9 to 
                     confirm C-term SV40 and HA, and N-term SunTag; Plasmid: 
                     60910; Source: CLC"
     misc_feature    9241..9501
                     /label=UGI
     primer_bind     9324..9337
                     /label=pongTpase_5R
                     /note="Primer P1405, Plasmid 47101, Comments: Rev 
                     primer--anneals ~bp7240 of Tpase. ; Parrot lab (mp)"
     primer_bind     9417..9428
                     /label=#28 HA-F
                     /note="Created from HA sequence: for sequencing plasmids 
                     with N-terminal HA tag, but note that some HA sequences may
                     vary; HA tag, forward primer. Created by: Sharon"
     CDS             9502..9522
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(9595..9614)
                     /label=P1925 E9-term-R
                     /note="Comments: Reverse primer for E9 terminator (from pea
                     rbcS-E9 gene). Anneals to 5' end of terminator and 
                     sequences in reverse direction.; Plasmid: Deposit 72127; 
                     Source: JN"
     primer_bind     complement(10180..10196)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10193..10216)
                     /label=#102 Tac promoter
                     /note="Source: David Waugh's lab; Tac promoter, forward 
                     primer. Created by: Depositor"
     primer_bind     complement(10193..10215)
                     /label=#45 M13pUC-Rev
                     /note="5'-primer, binds to the lac/tac-operator region of 
                     many vectors (pProEX-HTabc, pUC8,9,13,18,19,57), reads 
                     downstream; In lacZ gene, also called M13 pUC Reverse. 
                     Created by: Invitrogen"
     primer_bind     complement(10194..10213)
                     /label=pQE-F2
                     /note="Primer P1418, Plasmid 48732, Comments: Forward 
                     primer, binds upstream of the MCS in the lac/tac operator 
                     region; for all pQE-vectors (Qiagen) lacking the DHFR-gene;
                     called TypeIII/IV by Sequiserve; Sequiserve (JN)"
     primer_bind     complement(10198..10217)
                     /label=pBMTL-1-5
                     /note="Primer P359, Plasmid 22811, Comments: Recommended 
                     primer for new vector; Gill lab"
     protein_bind    10204..10220
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10212..10231
                     /label=pCATseqR
                     /note="Primer P109, Plasmid 14590, Comments: pG6L6 CAT; 
                     designed by LS"
     promoter        complement(10228..10258)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(10235..10254)
                     /label=Lac-promoter
                     /note="P29"
     primer_bind     10239..10258
                     /label=#177 lac-promoter-R
                     /note="27396 and also general Lac promoter reverse primer."
     primer_bind     complement(10262..10273)
                     /label=pBbA5c-5
                     /note="Primer P949, Plasmid 35150-2, Comments: Forward 
                     primer for pBbA5c vector; Taek Soon Lee lab"
     protein_bind    10273..10294
                     /label=CAP bindin
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     10375..10393
                     /label=pRcoM1bs-R
                     /note="Primer P1017, Plasmid 69595, Comments: Reverse into 
                     Rcom1 binding sites ; AEB"
     promoter        10485..11161
                     /label=CaMV 35S promoter (enhanced) promoter
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(10563..10584)
                     /label=P1970 CaMV35S-R
                     /note="Comments: reverse primer recognizing CaMV 35S 
                     promoter region to verify upstream of the promoter.; 
                     Plasmid: deposit 72157; Source: MGL"
     primer_bind     complement(10888..10909)
                     /label=P1970 CaMV35S-R
                     /note="Comments: reverse primer recognizing CaMV 35S 
                     promoter region to verify upstream of the promoter.; 
                     Plasmid: deposit 72157; Source: MGL"
     primer_bind     11137..11156
                     /label=P1968 CaMV35S-F
                     /note="Comments: Sequence forw from the plant CaMV 35S 
                     promoter; Plasmid: deposit 72257; Source: JM"
     CDS             11228..12253
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     primer_bind     complement(11345..11364)
                     /label=#180 Hygro-R
                     /note="general; Hygromycin B reverse primer; anneals to 5' 
                     end of hygromycin; used pBABE-hygro as template"
     primer_bind     11385..11404
                     /label=P1724 Hygro-internal-F
                     /note="Comments: Hygromycin internal primer, anneals ~160bp
                     from 5' end and sequences through hygromycin in forward 
                     direction to confirm hygro sequence; Plasmid: 19066; 
                     Source: JN"
     primer_bind     11861..11870
                     /label=mREST-R
                     /note="Primer P242, Plasmid 21311, Comments: One last shot 
                     at this plasmid; EJP"
     primer_bind     complement(11975..11992)
                     /label=hCLSPN-F
                     /note="Primer P819, Plasmid 31319 (and others), Comments: 
                     Confirm mutations in human Claspin gene; EJP"
     primer_bind     12054..12065
                     /label=P1422 CeEgl-13_NLS-R
                     /note="Comments: Reverse primer from the C. Elegans 3' 
                     egl-13 NLS; Plasmid: 47944, 47945, 47946, 47947 (Deposit 
                     70450); Source: AMJ"
     primer_bind     12065..12083
                     /label=#179 Hygro-F
                     /note="general; Hygromycin B forward primer; anneals to 3' 
                     end of hygromycin; used pBABE-hygro as template."
     polyA_signal    12294..12468
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(12536..12556)
                     /label=LB-F1
                     /note="Primer P1392, Plasmid Deposit 70459 (MoClo Kit), 
                     Comments: Primer for LB (left border) element in 
                     Marillonnet Mo Clo system vectors; Sylvestre Marillonnet 
                     lab (AMJ)"
     misc_feature    12546..12570
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             12995..14566
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKGNDSNLLIVFYVQIMPDDFVMQLHRF*ERQRLPSQPCQVLPQ
                     IQVMPLNSLRISLADYVQLSLQAGFIQRPAIRHPYHHVKG*QQAHKTPQRRHSAFTEYV
                     RNNRLPETVIRVKQPALARFSPDIAPLFVHFRADDDVTARLYARGYRLRPEFFK*RKIV
                     LRPTPIMRAVARHPTPFMAISMIFWCVPG*EAV*VNCSCHVLRQ*EQR*R*CPAVLLPL
                     RTTPSVAEQEGQLIETEATGAPQKHHHTLNQ*VGSITMAKMRISPELKKLIEKYRCVKD
                     TEGMSPAKVYKLVGENENLYLKMTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFER
                     HDGWSNLLMSEADGVLCSEEYEDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRL
                     AELDYLLNNDLADVDCENWEEDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVK
                     DGKVSGFIDLGRSGRADKWYDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYI
                     LLDELF"
     primer_bind     complement(13910..13930)
                     /label=P1877 KanR1-R1
                     /note="Comments: Reverse primer from the 5' end of the R1 
                     permutation of Kan resistance; Plasmid: 66811; Source: AMJ"
     primer_bind     14339..14358
                     /label=KanR1-F1
                     /note="Primer P1360, Plasmid 46881, Comments: Fwd primer 
                     from 3' end of the R1 permutation of Kan resistance; AMJ"
     primer_bind     14512..14531
                     /label=pTC14_F2
                     /note="Primer P691, Plasmid TALEN project, Comments: 5' 
                     sequence primer for pTC14 vector; Colby Starker"
     rep_origin      14653..15241
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14678..14697)
                     /label=pBR322ori-3
                     /note="Primer P1021, Plasmid 37060, Comments: Binds 3' end 
                     of pBR322ori and reads out--verify MCS; mp"
     primer_bind     15142..15161
                     /label=#125 pBR322ori-F
                     /note="pBRS322 origin forward primer"
     primer_bind     15182..15201
                     /label=pRS414-R
                     /note="Primer P380, Plasmid 23081 and others, Comments: To 
                     confirm H4 in Dent lab plasmids; Dent lab"
     primer_bind     15255..15274
                     /label=ASK-FN2
                     /note="Primer P1160, Plasmid tbd, Comments: Universal (?) F
                     primer, needed for four plasmids in the Veit Hornung talen 
                     batch deposit; (AMJ)"
     primer_bind     complement(15278..15288)
                     /label=URA3-F
                     /note="Primer P201, Plasmid 21699..., Comments: 
                     Differentiating pFP series from Haber lab; HS"
     primer_bind     15341..15360
                     /label=RNAde-BB-2R
                     /note="Primer P1067, Plasmid Deposit 69711, Comments: 
                     sequence Jaramillo Biobrick vectors, 3' end; Jaramillo Lab 
                     (AMJ)"
     primer_bind     15341..15360
                     /label=pBbC6c-R
                     /note="Primer P1280, Plasmid 45152, Comments: Reverse 
                     primer aprox. 50bp downstream of insert, to pick up the 
                     C-term LVA; EJP"
     primer_bind     15358..15385
                     /label=ColEterm
                     /note="Primer P1043, Plasmid dep 62623, Comments: Verify 
                     Han Lim lab plasmids; Lim Lab"
     primer_bind     15373..15392
                     /label=ColE2
                     /note="Primer P808, Plasmid 32177, Comments: to confirm 
                     plasmids from deposit 69216; Niederweis Lab (mp)"
     primer_bind     15395..15412
                     /label=#34 L4440
                     /note="From Caldwell lab; 5' of MCS in L4440 vector, 
                     forward primer. Created by: Depositor"
     primer_bind     15400..15422
                     /label=pMOD4-5
                     /note="Primer P149, Plasmid 19182-3,5, Comments: pMOD4 
                     galK-G> Fisher Lab"
     primer_bind     15407..15426
                     /label=MoCloF
                     /note="Primer P1390, Plasmid Deposit 70459 (MoClo Kit), 
                     Comments: Fwd primer for Marillonnet Mo Clo system vectors;
                     Sylvestre Marillonnet lab (AMJ)"
     misc_feature    15427..15567
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(15492..15512)
                     /label=pRSII-F1
                     /note="Primer P1141, Plasmid Deposit 69759, Comments: 
                     anneals at ~bp150 in pRSII vectors, to confirm auxotrophic 
                     selection markers; AMJ"
     primer_bind     complement(15493..15512)
                     /label=#85 pRS-marker
                     /note="At 151 of pRS314 (1-186 same in all pRS's); To 
                     sequence yeast selectable marker in pRS vectors. Created 
                     by: Sharon"
     primer_bind     15653..15675
                     /label=#74 pGEX-3
                     /note="3' of MCS in pGEX vectors, reverse primer; also 
                     called pGEX3, pGEX-3', pGEX-3. Created by: Unknown"
     primer_bind     complement(15756..15767)
                     /label=pJFRC-F
                     /note="Primer P511, Plasmid Rubin plasmids, Comments: For 
                     repeat regions in pJFRC vector; Rubin lab"
     rep_origin      15911..16105
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(16171..17244)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     primer_bind     16636..16645
                     /label=pCAX-F
                     /note="Primer P708, Plasmid 30137 and friends, Comments: 
                     from the 5' end of pCAX; Tracy Young-Pierce lab"
     primer_bind     16838..16849
                     /label=hFLT1-F
                     /note="Primer P1095, Plasmid 23912, Comments: anneals 
                     around bp 2000 of human FLT1; LS"
     primer_bind     complement(17358..17370)
                     /label=P2141 ChREBP-R1
                     /note="Comments: Internal primer to confirm mouse ChREBP 
                     for customer complaint, Plasmids: 39235, Source: MC"
     primer_bind     17509..17519
                     /label=mCherry-R
                     /note="Primer P189, Plasmid 20297, Comments: Reverse primer
                     from N-terminus of DsRed (mCherry?); LS"
     CDS             complement(17673..18302)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     primer_bind     18053..18065
                     /label=P1922 AllEos-R
                     /note="Comments: Reverse primer for all Davidson Eos 
                     variants; Plasmid: ; Source: JM"
     primer_bind     18129..18139
                     /label=pACLU
                     /note="P24"
     primer_bind     18484..18494
                     /label=ColEterm
                     /note="Primer P1043, Plasmid dep 62623, Comments: Verify 
                     Han Lim lab plasmids; Lim Lab"
     primer_bind     18970..18983
                     /label=P1730 biPGK-F
                     /note="Comments: depositor recommended for miR trap 
                     plasmids-forward primer; Plasmid: 60934, 61487, 61488; 
                     Source: BMP"
     primer_bind     19234..19243
                     /label=mFOXO1-R
                     /note="Primer P810, Plasmid 24379, Comments: to confirm 
                     that mFOXO1 is in-frame with GST; AMH"
     primer_bind     19540..19559
                     /label=RB-F1
                     /note="Primer P1393, Plasmid Deposit 70459 (MoClo Kit), 
                     Comments: Primer for RB (right border) element in 
                     Marillonnet Mo Clo system vectors; Sylvestre Marillonnet 
                     lab (AMJ)"
ORIGIN
        1 taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta
       61 aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc
      121 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      181 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      241 cgacgttgta aaacgacggc cagtgccaag cttagtaatt catccaggtc tccaagttct
      301 aggattttca gaactgcaac ttattttatc aaggaatctt taaacatacg aacagatcac
      361 ttaaagttct tctgaagcaa cttaaagtta tcaggcatgc atggatcttg gaggaatcag
      421 atgtgcagtc agggaccata gcacaagaca ggcgtcttct actggtgcta ccagcaaatg
      481 ctggaagccg ggaacactgg gtacgttgga aaccacgtga tgtgaagaag taagataaac
      541 tgtaggagaa aagcatttcg tagtgggcca tgaagccttt caggacatgt attgcagtat
      601 gggccggccc attacgcaat tggacgacaa caaagactag tattagtacc acctcggcta
      661 tccacataga tcaaagctga tttaaaagag ttgtgcagat gatccgtggc gtgagaccaa
      721 cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac
      781 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca
      841 tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag
      901 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg
      961 gcagtcgccc taaaacaaag ttaaacatca tgggggaagc ggtgatcgcc gaagtatcga
     1021 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
     1081 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc
     1141 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
     1201 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
     1261 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
     1321 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
     1381 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
     1441 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
     1501 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
     1561 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact
     1621 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt
     1681 atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc
     1741 actacgtgaa aggcgagatc accaaggtag tcggcaaata atgtctagct agaaattcgt
     1801 tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac taagcacata
     1861 attgctcaca gccaaactat caggtcaagt ctgcttttat tatttttaag cgtgcataat
     1921 aagccggtct cggttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca
     1981 acttgaaaaa gtggcaccga gtcggtgctt ttttttttcg ttttgcattg agttttctcc
     2041 gtcgcatgtt tgcagtttta ttttccgttt tgcattgaaa tttctccgtc tcatgtttgc
     2101 agcgtgttca aaaagtacgc agctgtattt cacttattta cggcgccaca ttttcatgcc
     2161 gtttgtgcca actatcccga gctagtgaat acagcttggc ttcacacaac actggtgacc
     2221 cgctgacctg ctcgtacctc gtaccgtcgt acggcacagc atttggaatt aaagggtgtg
     2281 atcgatactg cttgctgcta agcttgcatg cctgcagtgc agcgtgaccc ggtcgtgccc
     2341 ctctctagag ataatgagca ttgcatgtct aagttataaa aaattaccac atattttttt
     2401 tgtcacactt gtttgaagtg cagtttatct atctttatac atatatttaa actttactct
     2461 acgaataata taatctatag tactacaata atatcagtgt tttagagaat catataaatg
     2521 aacagttaga catggtctaa aggacaattg agtattttga caacaggact ctacagtttt
     2581 atctttttag tgtgcatgtg ttctcctttt tttttgcaaa tagcttcacc tatataatac
     2641 ttcatccatt ttattagtac atccatttag ggtttagggt taatggtttt tatagactaa
     2701 tttttttagt acatctattt tattctattt tagcctctaa attaagaaaa ctaaaactct
     2761 attttagttt ttttatttaa taatttagat ataaaataga ataaaataaa gtgactaaaa
     2821 attaaacaaa taccctttaa gaaattaaaa aaactaagga aacatttttc ttgtttcgag
     2881 tagataatgc cagcctgtta aacgccgtcg acgagtctaa cggacaccaa ccagcgaacc
     2941 agcagcgtcg cgtcgggcca agcgaagcag acggcacggc atctctgtcg ctgcctctgg
     3001 acccctctcg agagttccgc tccaccgttg gacttgctcc gctgtcggca tccagaaatt
     3061 gcgtggcgga gcggcagacg tgagccggca cggcaggcgg cctcctcctc ctctcacggc
     3121 accggcagct acgggggatt cctttcccac cgctccttcg ctttcccttc ctcgcccgcc
     3181 gtaataaata gacaccccct ccacaccctc tttccccaac ctcgtgttgt tcggagcgca
     3241 cacacacaca accagatctc ccccaaatcc acccgtcggc acctccgctt caaggtacgc
     3301 cgctcgtcct cccccccccc ccctctctac cttctctaga tcggcgttcc ggtccatggt
     3361 tagggcccgg tagttctact tctgttcatg tttgtgttag atccgtgttt gtgttagatc
     3421 cgtgctgcta gcgttcgtac acggatgcga cctgtacgtc agacacgttc tgattgctaa
     3481 cttgccagtg tttctctttg gggaatcctg ggatggctct agccgttccg cagacgggat
     3541 cgatttcatg attttttttg tttcgttgca tagggtttgg tttgcccttt tcctttattt
     3601 caatatatgc cgtgcacttg tttgtcgggt catcttttca tgcttttttt tgtcttggtt
     3661 gtgatgatgt ggtctggttg ggcggtcgtt ctagatcgga gtagaattct gtttcaaact
     3721 acctggtgga tttattaatt ttggatctgt atgtgtgtgc catacatatt catagttacg
     3781 aattgaagat gatggatgga aatatcgatc taggataggt atacatgttg atgcgggttt
     3841 tactgatgca tatacagaga tgctttttgt tcgcttggtt gtgatgatgt ggtgtggttg
     3901 ggcggtcgtt cattcgttct agatcggagt agaatactgt ttcaaactac ctggtgtatt
     3961 tattaatttt ggaactgtat gtgtgtgtca tacatcttca tagttacgag tttaagatgg
     4021 atggaaatat cgatctagga taggtataca tgttgatgtg ggttttactg atgcatatac
     4081 atgatggcat atgcagcatc tattcatatg ctctaacctt gagtacctat ctattataat
     4141 aaacaagtat gttttataat tattttgatc ttgatatact tggatgatgg catatgcagc
     4201 agctatatgt ggattttttt agccctgcct tcatacgcta tttatttgct tggtactgtt
     4261 tcttttgtcg atgctcaccc tgttgtttgg tgttacttct gcagccctag gatgccaaag
     4321 aagaagagga aggtttcatc ggagaccggc cctgttgctg ttgaccccac cctgcggcgg
     4381 agaatcgagc cacacgagtt cgaggtgttc ttcgacccaa gggagctccg caaggagacg
     4441 tgcctcctgt acgagatcaa ctggggcggc aggcactcca tctggaggca caccagccaa
     4501 aacaccaaca agcacgtgga ggtcaacttc atcgagaagt tcaccaccga gaggtacttc
     4561 tgcccaaaca cccgctgctc catcacctgg ttcctgtcct ggagcccatg cggcgagtgc
     4621 tccagggcca tcaccgagtt cctcagccgc tacccacacg tcaccctgtt catctacatc
     4681 gccaggctct accaccacgc cgacccaagg aacaggcagg gcctccgcga cctgatctcc
     4741 agcggcgtga ccatccaaat catgaccgag caggagtccg gctactgctg gaggaacttc
     4801 gtcaactact ccccaagcaa cgaggcccac tggccaaggt acccacacct ctgggtgcgc
     4861 ctctacgtgc tcgagctgta ctgcatcatc ctcggcctgc caccatgcct caacatcctg
     4921 aggcgcaagc aaccacagct gaccttcttc accatcgccc tccaaagctg ccactaccag
     4981 aggctcccac cacacatcct gtgggctacc ggcctcaagt ccggcagcga gacgccaggc
     5041 acctccgaga gcgctacgcc tgaacttaag gacaagaagt actcgatcgg cctcgccatc
     5101 gggacgaact cagttggctg ggccgtgatc accgacgagt acaaggtgcc ctctaagaag
     5161 ttcaaggtcc tggggaacac cgaccgccat tccatcaaga agaacctcat cggcgctctc
     5221 ctgttcgaca gcggggagac cgctgaggct acgaggctca agagaaccgc taggcgccgg
     5281 tacacgagaa ggaagaacag gatctgctac ctccaagaga ttttctccaa cgagatggcc
     5341 aaggttgacg attcattctt ccaccgcctg gaggagtctt tcctcgtgga ggaggataag
     5401 aagcacgagc ggcatcccat cttcggcaac atcgtggacg aggttgccta ccacgagaag
     5461 taccctacga tctaccatct gcggaagaag ctcgtggact ccaccgataa ggcggacctc
     5521 agactgatct acctcgctct ggcccacatg atcaagttcc gcggccattt cctgatcgag
     5581 ggggatctca acccagacaa cagcgatgtt gacaagctgt tcatccaact cgtgcagacc
     5641 tacaaccaac tcttcgagga gaacccgatc aacgcctctg gcgtggacgc gaaggctatc
     5701 ctgtccgcga ggctctcgaa gtccaggagg ctggagaacc tgatcgctca gctcccaggc
     5761 gagaagaaga acggcctgtt cgggaacctc atcgctctca gcctggggct caccccgaac
     5821 ttcaagtcga acttcgatct cgctgaggac gccaagctgc aactctccaa ggacacctac
     5881 gacgatgacc tcgataacct cctggcccag atcggcgatc aatacgcgga cctgttcctc
     5941 gctgccaaga acctgtcgga cgccatcctc ctgtcagata tcctccgcgt gaacaccgag
     6001 atcacgaagg ctccactctc tgcctccatg atcaagcgct acgacgagca ccatcaggat
     6061 ctgaccctcc tgaaggcgct ggtccgccaa cagctcccgg agaagtacaa ggagattttc
     6121 ttcgatcagt cgaagaacgg ctacgctggg tacatcgacg gcggggcctc acaagaggag
     6181 ttctacaagt tcatcaagcc aatcctggag aagatggacg gcacggagga gctcctggtg
     6241 aagctcaaca gggaggacct cctgcggaag cagagaacct tcgataacgg cagcatcccc
     6301 caccaaatcc atctcgggga gctgcacgcc atcctgagaa ggcaagagga cttctaccct
     6361 ttcctcaagg ataaccggga gaagatcgag aagatcctga ccttcagaat cccatactac
     6421 gtcggccctc tcgcgcgggg gaactcaaga ttcgcttgga tgacccgcaa gtctgaggag
     6481 accatcacgc cgtggaactt cgaggaggtg gtggacaagg gcgctagcgc tcagtcgttc
     6541 atcgagagga tgaccaactt cgacaagaac ctgcccaacg agaaggtgct ccctaagcac
     6601 tcgctcctgt acgagtactt caccgtctac aacgagctca cgaaggtgaa gtacgtcacc
     6661 gagggcatgc gcaagccagc gttcctgtcc ggggagcaga agaaggctat cgtggacctc
     6721 ctgttcaaga ccaaccggaa ggtcacggtt aagcaactca aggaggacta cttcaagaag
     6781 atcgagtgct tcgattcggt cgagatcagc ggcgttgagg accgcttcaa cgccagcctc
     6841 gggacctacc acgatctcct gaagatcatc aaggataagg acttcctgga caacgaggag
     6901 aacgaggata tcctggagga catcgtgctg accctcacgc tgttcgagga cagggagatg
     6961 atcgaggagc gcctgaagac gtacgcccat ctcttcgatg acaaggtcat gaagcaactc
     7021 aagcgccgga gatacaccgg ctgggggagg ctgtcccgca agctcatcaa cggcatccgg
     7081 gacaagcagt ccgggaagac catcctcgac ttcctcaaga gcgatggctt cgccaacagg
     7141 aacttcatgc aactgatcca cgatgacagc ctcaccttca aggaggatat ccaaaaggct
     7201 caagtgagcg gccaggggga ctcgctgcac gagcatatcg cgaacctcgc tggctccccc
     7261 gcgatcaaga agggcatcct ccagaccgtg aaggttgtgg acgagctcgt gaaggtcatg
     7321 ggccggcaca agcctgagaa catcgtcatc gagatggcca gagagaacca aaccacgcag
     7381 aaggggcaaa agaactctag ggagcgcatg aagcgcatcg aggagggcat caaggagctg
     7441 gggtcccaaa tcctcaagga gcacccagtg gagaacaccc aactgcagaa cgagaagctc
     7501 tacctgtact acctccagaa cggcagggat atgtacgtgg accaagagct ggatatcaac
     7561 cgcctcagcg attacgacgt cgatcatatc gttccccagt ctttcctgaa ggatgactcc
     7621 atcgacaaca aggtcctcac caggtcggac aagaaccgcg gcaagtcaga taacgttcca
     7681 tctgaggagg tcgttaagaa gatgaagaac tactggaggc agctcctgaa cgccaagctg
     7741 atcacgcaaa ggaagttcga caacctcacc aaggctgaga gaggcgggct ctcagagctg
     7801 gacaaggccg gcttcatcaa gcggcagctg gtcgagacca gacaaatcac gaagcacgtt
     7861 gcgcaaatcc tcgactctcg gatgaacacg aagtacgatg agaacgacaa gctgatcagg
     7921 gaggttaagg tgatcaccct gaagtctaag ctcgtctccg acttcaggaa ggatttccag
     7981 ttctacaagg ttcgcgagat caacaactac caccatgccc atgacgctta cctcaacgct
     8041 gtggtcggca ccgctctgat caagaagtac ccaaagctgg agtccgagtt cgtgtacggg
     8101 gactacaagg tttacgatgt gcgcaagatg atcgccaagt cggagcaaga gatcggcaag
     8161 gctaccgcca agtacttctt ctactcaaac atcatgaact tcttcaagac cgagatcacg
     8221 ctggccaacg gcgagatccg gaagagaccg ctcatcgaga ccaacggcga gacgggggag
     8281 atcgtgtggg acaagggcag ggatttcgcg accgtccgca aggttctctc catgccccag
     8341 gtgaacatcg tcaagaagac cgaggtccaa acgggcgggt tctcaaagga gtctatcctg
     8401 cctaagcgga acagcgacaa gctcatcgcc agaaagaagg actgggaccc aaagaagtac
     8461 ggcgggttcg acagccctac cgtggcctac tcggtcctgg ttgtggcgaa ggttgagaag
     8521 ggcaagtcca agaagctcaa gagcgtgaag gagctcctgg ggatcaccat catggagagg
     8581 tccagcttcg agaagaaccc aatcgacttc ctggaggcca agggctacaa ggaggtgaag
     8641 aaggacctga tcatcaagct cccgaagtac tctctcttcg agctggagaa cggcaggaag
     8701 agaatgctgg cttccgctgg cgagctccag aaggggaacg agctcgcgct gccaagcaag
     8761 tacgtgaact tcctctacct ggcttcccac tacgagaagc tcaagggcag cccggaggac
     8821 aacgagcaaa agcagctgtt cgtcgagcag cacaagcatt acctcgacga gatcatcgag
     8881 caaatctccg agttcagcaa gcgcgtgatc ctcgccgacg cgaacctgga taaggtcctc
     8941 tccgcctaca acaagcaccg ggacaagccc atcagagagc aagcggagaa catcatccat
     9001 ctcttcaccc tgacgaacct cggcgctcct gctgctttca agtacttcga caccacgatc
     9061 gatcggaaga gatacacctc cacgaaggag gtcctggacg cgaccctcat ccaccagtcg
     9121 atcaccggcc tgtacgagac gaggatcgac ctctcacaac tcggcgggga taagagaccc
     9181 gcagcaacca agaaggcagg gcaagcaaag aagaagaaga cgcgtgactc cggcggcagc
     9241 accaacctgt ccgacatcat cgagaaggag acgggcaagc aactcgtgat ccaggagagc
     9301 atcctcatgc tgccagagga ggtggaggag gtcatcggca acaagccaga gtccgacatc
     9361 ctggtgcaca ccgcctacga cgagtccacc gacgagaacg tcatgctcct gaccagcgac
     9421 gccccagagt acaagccatg ggccctcgtc atccaggaca gcaacgggga gaacaagatc
     9481 aagatgctgt cgggggggag cccaaagaag aagcggaagg tgtagagctc agagctttcg
     9541 ttcgtatcat cggtttcgac aacgttcgtc aagttcaatg catcagtttc attgcgcaca
     9601 caccagaatc ctactgagtt tgagtattat ggcattggga aaactgtttt tcttgtacca
     9661 tttgttgtgc ttgtaattta ctgtgttttt tattcggttt tcgctatcga actgtgaaat
     9721 ggaaatggat ggagaagagt taatgaatga tatggtcctt ttgttcattc tcaaattaat
     9781 attatttgtt ttttctctta tttgttgtgt gttgaatttg aaattataag agatatgcaa
     9841 acattttgtt ttgagtaaaa atgtgtcaaa tcgtggcctc taatgaccga agttaatatg
     9901 aggagtaaaa cacttgtagt tgtaccatta tgcttattca ctaggcaaca aatatatttt
     9961 cagacctaga aaagctgcaa atgttactga atacaagtat gtcctcttgt gttttagaca
    10021 tttatgaact ttcctttatg taattttcca gaatccttgt cagattctaa tcattgcttt
    10081 ataattatag ttatactcat ggatttgtag ttgagtatga aaatattttt taatgcattt
    10141 tatgacttgc caattgattg acaacgaatt cgtaatcatg tcatagctgt ttcctgtgtg
    10201 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
    10261 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
    10321 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
    10381 cggtttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac
    10441 tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa
    10501 agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa
    10561 aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct
    10621 atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc
    10681 atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgaacat
    10741 ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct cagaagacca
    10801 aagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg gattccattg
    10861 cccagctatc tgtcacttca tcaaaaggac agtagaaaag gaaggtggca cctacaaatg
    10921 ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctgccgaca gtggtcccaa
    10981 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
    11041 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
    11101 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga ggacacgctg
    11161 aaatcaccag tctctctcta caaatctatc tctctcgagc tttcgcagat ccggggggca
    11221 atgagatatg aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa
    11281 gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag
    11341 cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta
    11401 caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct
    11461 tgacattggg gagtttagcg agagcctgac ctattgcatc tcccgccgtt cacagggtgt
    11521 cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctacaaccgg tcgcggaggc
    11581 tatggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc
    11641 gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca
    11701 tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct
    11761 cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga
    11821 tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag
    11881 cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg
    11941 gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg
    12001 atcgccacga ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt
    12061 ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg
    12121 atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac
    12181 cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag
    12241 ggcaaagaaa tagagtagat gccgaccggg atctgtcgat cgacaagctc gagtttctcc
    12301 ataataatgt gtgagtagtt cccagataag ggaattaggg ttcctatagg gtttcgctca
    12361 tgtgttgagc atataagaaa cccttagtat gtatttgtat ttgtaaaata cttctatcaa
    12421 taaaatttct aattcctaaa accaaaatcc agtactaaaa tccagatccc ccgaattaat
    12481 tcggcgttaa ttcagtacat taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc
    12541 aatttgttta caccacaata tatcctgcca ccagccagcc aacagctccc cgaccggcag
    12601 ctcggcacaa aatcaccact cgatacaggc agcccatcag tccgggacgg cgtcagcggg
    12661 agagccgttg taaggcggca gactttgctc atgttaccga tgctattcgg aagaacggca
    12721 actaagctgc cgggtttgaa acacggatga tctcgcggag ggtagcatgt tgattgtaac
    12781 gatgacagag cgttgctgcc tgtgatcacc gcggtttcaa aatcggctcc gtcgatacta
    12841 tgttatacgc caactttgaa aacaactttg aaaaagctgt tttctggtat ttaaggtttt
    12901 agaatgcaag gaacagtgaa ttggagttcg tcttgttata attagcttct tggggtatct
    12961 ttaaatactg tagaaaagag gaaggaaata ataaatggct aaaggtaatg actccaactt
    13021 attgatagtg ttttatgttc agataatgcc cgatgacttt gtcatgcagc tccaccgatt
    13081 ttgagaacga cagcgacttc cgtcccagcc gtgccaggtg ctgcctcaga ttcaggttat
    13141 gccgctcaat tcgctgcgta tatcgcttgc tgattacgtg cagctttccc ttcaggcggg
    13201 attcatacag cggccagcca tccgtcatcc atatcaccac gtcaaagggt gacagcaggc
    13261 tcataagacg ccccagcgtc gccatagtgc gttcaccgaa tacgtgcgca acaaccgtct
    13321 tccggagact gtcatacgcg taaaacagcc agcgctggcg cgatttagcc ccgacatagc
    13381 cccactgttc gtccatttcc gcgcagacga tgacgtcact gcccggctgt atgcgcgagg
    13441 ttaccgactg cggcctgagt tttttaagtg acgtaaaatc gtgttgaggc caacgcccat
    13501 aatgcgggct gttgcccggc atccaacgcc attcatggcc atatcaatga ttttctggtg
    13561 cgtaccgggt tgagaagcgg tgtaagtgaa ctgcagttgc catgttttac ggcagtgaga
    13621 gcagagatag cgctgatgtc cggcggtgct tttgccgtta cgcaccaccc cgtcagtagc
    13681 tgaacaggag ggacagctga tagaaacaga agccactgga gcacctcaaa aacaccatca
    13741 tacactaaat cagtaagttg gcagcatcac catggctaaa atgagaatat caccggaatt
    13801 gaaaaaactg atcgaaaaat accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa
    13861 ggtatataag ctggtgggag aaaatgaaaa cctatattta aaaatgacgg acagccggta
    13921 taaagggacc acctatgatg tggaacggga aaaggacatg atgctatggc tggaaggaaa
    13981 gctgcctgtt ccaaaggtcc tgcactttga acggcatgat ggctggagca atctgctcat
    14041 gagtgaggcc gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa
    14101 gattatcgag ctgtatgcgg agtgcatcag gctctttcac tccatcgaca tatcggattg
    14161 tccctatacg aatagcttag acagccgctt agccgaattg gattacttac tgaataacga
    14221 tctggccgat gtggattgcg aaaactggga agaagacact ccatttaaag atccgcgcga
    14281 gctgtatgat tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt cccacggcga
    14341 cctgggagac agcaacatct ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg
    14401 gagaagcggc agggcggaca agtggtatga cattgccttc tgcgtccggt cgatcaggga
    14461 ggatatcggg gaagaacagt atgtcgagct attttttgac ttactgggga tcaagcctga
    14521 ttgggagaaa ataaaatatt atattttact ggatgaattg ttttagtacc tagaatgcat
    14581 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
    14641 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
    14701 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
    14761 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
    14821 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
    14881 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
    14941 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
    15001 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
    15061 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
    15121 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
    15181 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
    15241 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
    15301 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
    15361 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
    15421 agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg
    15481 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta
    15541 tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc
    15601 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
    15661 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcaggg tgccttgatg
    15721 tgggcgccgg cggtcgagtg gcgacggcgc ggcttgtccg cgccctggta gattgcctgg
    15781 ccgtaggcca gccatttttg agcggccagc ggccgcgata ggccgacgcg aagcggcggg
    15841 gcgtagggag cgcagcgacc gaagggtagg cgctttttgc agctcttcgg ctgtgcgctg
    15901 gccagacagt tatgcacagg ccaggcgggt tttaagagtt ttaataagtt ttaaagagtt
    15961 ttaggcggaa aaatcgcctt ttttctcttt tatatcagtc acttacatgt gtgaccggtt
    16021 cccaatgtac ggctttgggt tcccaatgta cgggttccgg ttcccaatgt acggctttgg
    16081 gttcccaatg tacgtgctat ccacaggaaa cagacctttt cgaccttttt cccctgctag
    16141 ggcaatttgc cctagcatct gctccgtaca ttaggaaccg gcggatgctt cgccctcgat
    16201 caggttgcgg tagcgcatga ctaggatcgg gccagcctgc cccgcctcct ccttcaaatc
    16261 gtactccggc aggtcatttg acccgatcag cttgcgcacg gtgaaacaga acttcttgaa
    16321 ctctccggcg ctgccactgc gttcgtagat cgtcttgaac aaccatctgg cttctgcctt
    16381 gcctgcggcg cggcgtgcca ggcggtagag aaaacggccg atgccgggat cgatcaaaaa
    16441 gtaatcgggg tgaaccgtca gcacgtccgg gttcttgcct tctgtgatct cgcggtacat
    16501 ccaatcagct agctcgatct cgatgtactc cggccgcccg gtttcgctct ttacgatctt
    16561 gtagcggcta atcaaggctt caccctcgga taccgtcacc aggcggccgt tcttggcctt
    16621 cttcgtacgc tgcatggcaa cgtgcgtggt gtttaaccga atgcaggttt ctaccaggtc
    16681 gtctttctgc tttccgccat cggctcgccg gcagaacttg agtacgtccg caacgtgtgg
    16741 acggaacacg cggccgggct tgtctccctt cccttcccgg tatcggttca tggattcggt
    16801 tagatgggaa accgccatca gtaccaggtc gtaatcccac acactggcca tgccggccgg
    16861 ccctgcggaa acctctacgt gcccgtctgg aagctcgtag cggatcacct cgccagctcg
    16921 tcggtcacgc ttcgacagac ggaaaacggc cacgtccatg atgctgcgac tatcgcgggt
    16981 gcccacgtca tagagcatcg gaacgaaaaa atctggttgc tcgtcgccct tgggcggctt
    17041 cctaatcgac ggcgcaccgg ctgccggcgg ttgccgggat tctttgcgga ttcgatcagc
    17101 ggccgcttgc cacgattcac cggggcgtgc ttctgcctcg atgcgttgcc gctgggcggc
    17161 ctgcgcggcc ttcaacttct ccaccaggtc atcacccagc gccgcgccga tttgtaccgg
    17221 gccggatggt ttgcgaccgc tcacgccgat tcctcgggct tgggggttcc agtgccattg
    17281 cagggccggc aggcaaccca gccgcttacg cctggccaac cgcccgttcc tccacacatg
    17341 gggcattcca cggcgtcggt gcctggttgt tcttgatttt ccatgccgcc tcctttagcc
    17401 gctaaaattc atctactcat ttattcattt gctcatttac tctggtagct gcgcgatgta
    17461 ttcagatagc agctcggtaa tggtcttgcc ttggcgtacc gcgtacatct tcagcttggt
    17521 gtgatcctcc gccggcaact gaaagttgac ccgcttcatg gctggcgtgt ctgccaggct
    17581 ggccaacgtt gcagccttgc tgctgcgtgc gctcggacgg ccggcactta gcgtgtttgt
    17641 gcttttgctc attttctctt tacctcatta actcaaatga gttttgattt aatttcagcg
    17701 gccagcgcct ggacctcgcg ggcagcgtcg ccctcgggtt ctgattcaag aacggttgtg
    17761 ccggcggcgg cagtgcctgg gtagctcacg cgctgcgtga tacgggactc aagaatgggc
    17821 agctcgtacc cggccagcgc ctcggcaacc tcaccgccga tgcgcgtgcc tttgatcgcc
    17881 cgcgacacga caaaggccgc ttgtagcctt ccatccgtga cctcaatgcg ctgcttaacc
    17941 agctccacca ggtcggcggt ggcccatatg tcgtaagggc ttggctgcac cggaatcagc
    18001 acgaagtcgg ctgccttgat cgcggacaca gccaagtccg ccgcctgggg cgctccgtcg
    18061 atcactacga agtcgcgccg gccgatggcc ttcacgtcgc ggtcaatcgt cgggcggtcg
    18121 atgccgacaa cggttagcgg ttgatcttcc cgcacggccg cccaatcgcg ggcactgccc
    18181 tggggatcgg aatcgactaa cagaacatcg gccccggcga gttgcagggc gcgggctaga
    18241 tgggttgcga tggtcgtctt gcctgacccg cctttctggt taagtacagc gataaccttc
    18301 atgcgttccc cttgcgtatt tgtttattta ctcatcgcat catatacgca gcgaccgcat
    18361 gacgcaagct gttttactca aatacacatc acctttttag acggcggcgc tcggtttctt
    18421 cagcggccaa gctggccggc caggccgcca gcttggcatc agacaaaccg gccaggattt
    18481 catgcagccg cacggttgag acgtgcgcgg gcggctcgaa cacgtacccg gccgcgatca
    18541 tctccgcctc gatctcttcg gtaatgaaaa acggttcgtc ctggccgtcc tggtgcggtt
    18601 tcatgcttgt tcctcttggc gttcattctc ggcggccgcc agggcgtcgg cctcggtcaa
    18661 tgcgtcctca cggaaggcac cgcgccgcct ggcctcggtg ggcgtcactt cctcgctgcg
    18721 ctcaagtgcg cggtacaggg tcgagcgatg cacgccaagc agtgcagccg cctctttcac
    18781 ggtgcggcct tcctggtcga tcagctcgcg ggcgtgcgcg atctgtgccg gggtgagggt
    18841 agggcggggg ccaaacttca cgcctcgggc cttggcggcc tcgcgcccgc tccgggtgcg
    18901 gtcgatgatt agggaacgct cgaactcggc aatgccggcg aacacggtca acaccatgcg
    18961 gccggccggc gtggtggtgt cggcccacgg ctctgccagg ctacgcaggc ccgcgccggc
    19021 ctcctggatg cgctcggcaa tgtccagtag gtcgcgggtg ctgcgggcca ggcggtctag
    19081 cctggtcact gtcacaacgt cgccagggcg taggtggtca agcatcctgg ccagctccgg
    19141 gcggtcgcgc ctggtgccgg tgatcttctc ggaaaacagc ttggtgcagc cggccgcgtg
    19201 cagttcggcc cgttggttgg tcaagtcctg gtcgtcggtg ctgacgcggg catagcccag
    19261 caggccagcg gcggcgctct tgttcatggc gtaatgtctc cggttctagt cgcaagtatt
    19321 ctactttatg cgactaaaac acgcgacaag aaaacgccag gaaaagggca gggcggcagc
    19381 ctgtcgcgta acttaggact tgtgcgacat gtcgttttca gaagacggct gcactgaacg
    19441 tcagaagccg actgcactat agcagcggag gggttggatc aaagtacttt gatcccgagg
    19501 ggaaccctgt ggttggcatg cacatacaaa tggacgaacg gataaacctt ttcacgccct
    19561 tttaaatatc cgattattct aa
//
