LOCUS pAAVsynFlexRGC-W.xdna 6638 bp DNA circular 24/07/2017 DEFINITION ACCESSION VERSION SOURCE ORGANISM COMMENT Serial Cloner Genbank Format COMMENT SerialCloner_Type=DNA COMMENT SerialCloner_Comments=Ligation of : pAAV-Syn-flex RGC-W.xdna [6249 nt] : (NsiI[36 53] / EcoRI[3315]) to pAAV-MCS.xdna [381 nt] : (EcoRI[1326] / NsiI[1711]) COMMENT SerialCloner_Ends=0,0,,0, FEATURES Location/Qualifiers misc_feature 643..676 /label=lox2272f /SerialCloner_Color=&h736CAD /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 739..772 /label=loxpf /SerialCloner_Color=&h860EEE /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature complement(2440..3151) /label=cerulean /SerialCloner_Color=&h4096DC /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=note="opposite strand translation"translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKL \ misc_feature complement(2363..2439) /label=E2A /SerialCloner_Color=&hEB4E18 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=note="opposite strand translation"translation="RLAVVVNVLTTLC*" \ misc_feature complement(785..2362) /label=RG /SerialCloner_Color=&hE735D7 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=note="opposite strand translation"translation="SGVPQALLFVPLLVFPLCFGKFPIYTIPDKLGPWSPIDIHHLSCPNNLVVEDEGCTNLSGFSYMELKVGYILAIKVNGFTCTGVVTEAETYTNFVGYVTTTFKRKHFRPTPDACRAAYNWKMAGDPRYEESLHNPYPDYRWLRTVKTTKESLVIISPSVADLDPYDRSLHSRVFPSGKCSGVAVSSTYCSTNHDYTIWMPENPRLGM \ rep_origin 5968..6596 /label=ColE1 origin /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True rep_origin complement(4122..4562) /label=F1 ori /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True rep_origin 4117..4572 /label=M13 origin /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True CDS 5157..5816 /label=AmpR /SerialCloner_Color=&h8FBC8F /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True polyA_signal 3385..3865 /label=hGH polyA signal /SerialCloner_Color=&hFFC0CB /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True misc_recomb 3264..3297 /label=Lox2272r /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 162..635 /label=hSyn /SerialCloner_Color=&hC07510 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=note="Fragment extracted from pAAV-hSYN-Flex-GTB.xdna [635 nt] : (PciI[7749] / NcoI[634]) \ misc_feature 3160..3193 /label=loxpr /SerialCloner_Color=&h6064C0 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True 3320..3700 /label=insert from pAAV-MCS.xdna /SerialCloner_Color=&h84A4C0 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=note="Fragment extracted from pAAV-MCS.xdna [381 nt] : (EcoRI[1326] / NsiI[1711]) \ misc_feature 3902..4042 /label=itr /SerialCloner_Color=&h153CC0 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True ORIGIN 1 TCCTGCAGGC AGCTGCGCGC TCGCTCGCTC ACTGAGGCCG CCCGGGCAAA GCCCGGGCGT 61 CGGGCGACCT TTGGTCGCCC GGCCTCAGTG AGCGAGCGAG CGCGCAGAGA GGGAGTGGCC 121 AACTCCATCA CTAGGGGTTC CTGCGGCCGC ACGCGGGCCC Tgcgtatgag tgcaagtggg 181 ttttaggacc aggatgaggc ggggtggggg tgcctacctg acgaccgacc ccgacccact 241 ggacaagcac ccaaccccca ttccccaaat tgcgcatccc ctatcagaga gggggagggg 301 aaacaggatg cggcgaggcg cgtgcgcact gccagcttca gcaccgcgga cagtgccttc 361 gcccccgcct ggcggcgcgc gccaccgccg cctcagcact gaaggcgcgc tgacgtcact 421 cgccggtccc ccgcaaactc cccttcccgg ccaccttggt cgcgtccgcg ccgccgccgg 481 cccagccgga ccgcaccacg cgaggcgcga gatagggggg cacgggcgcg accatctgcg 541 ctgcggcgcc ggcgactcag cgctgcctca gtctgcggtg ggcagcggag gagtcgtgtc 601 gtgcctgaga gcgcagtcga gaaaccggtC GCCACcatgG CCATAACTTC GTATAAAGTA 661 TCCTATACGA AGTTATATCA AAATAGGAAG ACCAATGCTT CACCATCGAC CCGAATTGCC 721 AAGCATCACC ATCGACCCAT AACTTCGTAT AATGTATGCT ATACGAAGTT ATACTTCAAC 781 TAGTTCACAG TCTGGTCTCG CCGCCGCTCT TGTGGCTCTC CCAGCTGCTG ATGATCTTGC 841 CGCTCTGGGG GGTCACGCTC ACCTCTCTGC CGGTGCCTCT CAGGTTGTGC TGGGTGGGCT 901 CGCTTCTGTT CACTCTTCTG CAGCAGGTCA TCAGGAAGAT GATCAGCATC AGGGCGGTCA 961 GGGCGCCGGC GCTCAGCAGC ACGTACTTGC CCCAGTTGGG CAGGCCCAGG TCCACGCCGC 1021 TCACCTGGTT GTGCACGTCG GGCAGGTGCA CCTCCACGAA GTCCTCGGCC TCGTCGCCGT 1081 CCTTGAACAC GGTGCTGGGG TCGGCCAGGG GGTGCACCAG GGGGATCACG CTGCTCTCCA 1141 GCAGCTCCAT GTGCTGCTGC AGCAGGCTGC TCTGCATCTC GGGGATCAGC ACGTTGCCGT 1201 CGGGGCCCAG GATGATGCCG TTGAAGAACA CGCCGTTCAC GTGGGGGTGG CATCTGCCGC 1261 CCACTCTCAG GCAGCCCTTG CTGGGCAGGA TCTCGTTCCA GGTTCTCACG CTCTTGTAGT 1321 GGGCGTCGGC CTCCATCAGG GTCTTGTTGA AGATGGTGTA GGCCTTGCCG AAGCCGGGCA 1381 CCAGCTTTCT CAGGTGGCTC AGTCTTCTGA AGCTCACGCT CTTGGTGGTC ATGATGCTCT 1441 CCAGGGCGTC CAGGCACTCC TCTCTCTTTC TCACCAGCTC CTCCACCACC AGGTGCTCGA 1501 TCTCGTCGCT TCTGAAGTCG TGCAGGTTCA CCAGCTTGTC GGGGGGGCAC CACTTGGTCT 1561 CGTTGCTGGT CTGCATGCTC ACCCAGGTGC CGTCCATCAG TCTCAGGCCC AGCACGCCGC 1621 ACAGCTTCAG CTTGCAGGCG CCCTTCAGGC TCTTGTACAG GCCTCTCTCG TCCACGAAGC 1681 CGCAGGTCTC GCTGCCCTTG CTGGCTCTCT TGCCTCTGCT GTTGGTGAAG ATGTCGCAGC 1741 TCATGCCCAG TCTGGGGTTC TCGGGCATCC AGATGGTGTA GTCGTGGTTG GTGCTGCAGT 1801 AGGTGCTGCT CACGGCCACG CCGCTGCACT TGCCGCTGGG GAACACTCTG CTGTGCAGGC 1861 TTCTGTCGTA GGGGTCCAGG TCGGCCACGC TGGGGCTGAT GATCACCAGG CTCTCCTTGG 1921 TGGTCTTCAC GGTTCTCAGC CATCTGTAGT CGGGGTAGGG GTTGTGCAGG CTCTCCTCGT 1981 ATCTGGGGTC GCCGGCCATC TTCCAGTTGT AGGCGGCTCT GCAGGCGTCG GGGGTGGGTC 2041 TGAAGTGCTT TCTCTTGAAG GTGGTGGTCA CGTAGCCCAC GAAGTTGGTG TAGGTCTCGG 2101 CCTCGGTCAC CACGCCGGTG CAGGTGAAGC CGTTCACCTT GATGGCCAGG ATGTAGCCCA 2161 CCTTCAGCTC CATGTAGCTG AAGCCGCTCA GGTTGGTGCA GCCCTCGTCC TCCACCACCA 2221 GGTTGTTGGG GCAGCTCAGG TGGTGGATGT CGATGGGGCT CCAGGGGCCC AGCTTGTCGG 2281 GGATGGTGTA GATGGGGAAC TTGCCGAAGC ACAGGGGGAA CACCAGCAGG GGCACGAACA 2341 GCAGGGCCTG GGGCACTCCG GAAGGACCGG GGTTACTTTC AACATCGCCA GCGAGTTTCA 2401 ACAAAGCGTA GTTAGTACAT TGACCACTAC CGCTAGCctt gtacagctcg tccatgccga 2461 gagtgatccc ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg 2521 ggtctttgct cagcttggac tgggtgctca ggtagtggtt gtcgggcagc agcacggggc 2581 cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag ctgcacgctg ccgtcctcga 2641 tgttgtggcg gatcttgaag ttggccttga tgccgttctt ctgcttgtcg gcggtgatat 2701 agacgttgtc gctgatggcg ttgtactcca gcttgtgccc caggatgttg ccgtcctcct 2761 tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg aacttcacct 2821 cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc tggacgtagc 2881 cttcgggcat ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg tagcgggcga 2941 agcactgcac gccccaggtc agggtggtca cgagggtggg ccagggcacg ggcagcttgc 3001 cggtggtgca gatgaacttc agggtcagct tgccgtaggt ggcatcgccc tcgccctcgc 3061 cggacacgct gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg atgggCACCA 3121 CCCCGGTGAA CAGCTCCTCG CCCTTGCTCA CCTCGACGCA TAACTTCGTA TAGGATACTT 3181 TATACGAAGT TATCTTTGGG ATTCTTCCTA TTTTGATCCA AGCATCACCA TCGACCCTCT 3241 AGTCCAGATC TCACCATCGA CCCATAACTT CGTATAGCAT ACATTATACG AAGTTATGTC 3301 CCTCGAAGAG GTTCGaattC CCCGGGGATC CTCTAGAGTC GACCTGCAGA AGCTTGCCTC 3361 GAGCAGCGCT GCTCGAGAGA TCTACGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT 3421 CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG 3481 CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG 3541 GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG 3601 CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT 3661 TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA tgcaTGACCA GGCTCAGCTA 3721 ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC 3781 CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC 3841 CACTGCTCCC TTCCCTGTCC TTCTGATTTT GTAGGTAACC ACGTGCGGAC CGAGCGGCCG 3901 CAGGAACCCC TAGTGATGGA GTTGGCCACT CCCTCTCTGC GCGCTCGCTC GCTCACTGAG 3961 GCCGGGCGAC CAAAGGTCGC CCGACGCCCG GGCTTTGCCC GGGCGGCCTC AGTGAGCGAG 4021 CGAGCGCGCA GCTGCCTGCA GGGGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC 4081 GGTATTTCAC ACCGCATACG TCAAAGCAAC CATAGTACGC GCCCTGTAGC GGCGCATTAA 4141 GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC 4201 CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG 4261 CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA 4321 AAAAACTTGA TTTGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC 4381 GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA 4441 CACTCAACCC TATCTCGGGC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT 4501 ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTTAAC AAAATATTAA 4561 CGTTTACAAT TTTATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC 4621 AGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT 4681 CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT 4741 CATCACCGAA ACGCGCGAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG 4801 TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA 4861 CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC 4921 CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG 4981 TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC 5041 TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG 5101 ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA 5161 GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC 5221 AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG 5281 AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA 5341 GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG 5401 CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA 5461 ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT 5521 TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT 5581 GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT 5641 TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG 5701 GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA 5761 TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC 5821 TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA 5881 AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT 5941 TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT 6001 TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT 6061 GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC 6121 AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG 6181 TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG 6241 ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT 6301 CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC 6361 TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG 6421 ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG 6481 GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT 6541 TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT 6601 TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCAcatg //