LOCUS       pAAVsynFlexRGC-W.xdna    6638 bp      DNA     circular   24/07/2017
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=Ligation of : pAAV-Syn-flex RGC-W.xdna [6249  nt] : (NsiI[36
            53] / EcoRI[3315]) to pAAV-MCS.xdna [381  nt] : (EcoRI[1326]
             / NsiI[1711])
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     misc_feature    643..676
                     /label=lox2272f
                     /SerialCloner_Color=&h736CAD
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    739..772
                     /label=loxpf
                     /SerialCloner_Color=&h860EEE
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    complement(2440..3151)
                     /label=cerulean
                     /SerialCloner_Color=&h4096DC
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="opposite strand translation"translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKL \ 
     misc_feature    complement(2363..2439)
                     /label=E2A
                     /SerialCloner_Color=&hEB4E18
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="opposite strand translation"translation="RLAVVVNVLTTLC*" \ 
     misc_feature    complement(785..2362)
                     /label=RG
                     /SerialCloner_Color=&hE735D7
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="opposite strand translation"translation="SGVPQALLFVPLLVFPLCFGKFPIYTIPDKLGPWSPIDIHHLSCPNNLVVEDEGCTNLSGFSYMELKVGYILAIKVNGFTCTGVVTEAETYTNFVGYVTTTFKRKHFRPTPDACRAAYNWKMAGDPRYEESLHNPYPDYRWLRTVKTTKESLVIISPSVADLDPYDRSLHSRVFPSGKCSGVAVSSTYCSTNHDYTIWMPENPRLGM \ 
     rep_origin      5968..6596
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      complement(4122..4562)
                     /label=F1 ori
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      4117..4572
                     /label=M13 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             5157..5816
                     /label=AmpR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     polyA_signal    3385..3865
                     /label=hGH polyA signal
                     /SerialCloner_Color=&hFFC0CB
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_recomb     3264..3297
                     /label=Lox2272r
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    162..635
                     /label=hSyn
                     /SerialCloner_Color=&hC07510
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment extracted from pAAV-hSYN-Flex-GTB.xdna [635  nt] : (PciI[7749] / NcoI[634]) \ 
     misc_feature    3160..3193
                     /label=loxpr
                     /SerialCloner_Color=&h6064C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     3320..3700
                     /label=insert from pAAV-MCS.xdna
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment extracted from pAAV-MCS.xdna [381  nt] : (EcoRI[1326] / NsiI[1711]) \ 
     misc_feature    3902..4042
                     /label=itr
                     /SerialCloner_Color=&h153CC0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
ORIGIN
        1 TCCTGCAGGC AGCTGCGCGC TCGCTCGCTC ACTGAGGCCG CCCGGGCAAA GCCCGGGCGT 
       61 CGGGCGACCT TTGGTCGCCC GGCCTCAGTG AGCGAGCGAG CGCGCAGAGA GGGAGTGGCC 
      121 AACTCCATCA CTAGGGGTTC CTGCGGCCGC ACGCGGGCCC Tgcgtatgag tgcaagtggg 
      181 ttttaggacc aggatgaggc ggggtggggg tgcctacctg acgaccgacc ccgacccact 
      241 ggacaagcac ccaaccccca ttccccaaat tgcgcatccc ctatcagaga gggggagggg 
      301 aaacaggatg cggcgaggcg cgtgcgcact gccagcttca gcaccgcgga cagtgccttc 
      361 gcccccgcct ggcggcgcgc gccaccgccg cctcagcact gaaggcgcgc tgacgtcact 
      421 cgccggtccc ccgcaaactc cccttcccgg ccaccttggt cgcgtccgcg ccgccgccgg 
      481 cccagccgga ccgcaccacg cgaggcgcga gatagggggg cacgggcgcg accatctgcg 
      541 ctgcggcgcc ggcgactcag cgctgcctca gtctgcggtg ggcagcggag gagtcgtgtc 
      601 gtgcctgaga gcgcagtcga gaaaccggtC GCCACcatgG CCATAACTTC GTATAAAGTA 
      661 TCCTATACGA AGTTATATCA AAATAGGAAG ACCAATGCTT CACCATCGAC CCGAATTGCC 
      721 AAGCATCACC ATCGACCCAT AACTTCGTAT AATGTATGCT ATACGAAGTT ATACTTCAAC 
      781 TAGTTCACAG TCTGGTCTCG CCGCCGCTCT TGTGGCTCTC CCAGCTGCTG ATGATCTTGC 
      841 CGCTCTGGGG GGTCACGCTC ACCTCTCTGC CGGTGCCTCT CAGGTTGTGC TGGGTGGGCT 
      901 CGCTTCTGTT CACTCTTCTG CAGCAGGTCA TCAGGAAGAT GATCAGCATC AGGGCGGTCA 
      961 GGGCGCCGGC GCTCAGCAGC ACGTACTTGC CCCAGTTGGG CAGGCCCAGG TCCACGCCGC 
     1021 TCACCTGGTT GTGCACGTCG GGCAGGTGCA CCTCCACGAA GTCCTCGGCC TCGTCGCCGT 
     1081 CCTTGAACAC GGTGCTGGGG TCGGCCAGGG GGTGCACCAG GGGGATCACG CTGCTCTCCA 
     1141 GCAGCTCCAT GTGCTGCTGC AGCAGGCTGC TCTGCATCTC GGGGATCAGC ACGTTGCCGT 
     1201 CGGGGCCCAG GATGATGCCG TTGAAGAACA CGCCGTTCAC GTGGGGGTGG CATCTGCCGC 
     1261 CCACTCTCAG GCAGCCCTTG CTGGGCAGGA TCTCGTTCCA GGTTCTCACG CTCTTGTAGT 
     1321 GGGCGTCGGC CTCCATCAGG GTCTTGTTGA AGATGGTGTA GGCCTTGCCG AAGCCGGGCA 
     1381 CCAGCTTTCT CAGGTGGCTC AGTCTTCTGA AGCTCACGCT CTTGGTGGTC ATGATGCTCT 
     1441 CCAGGGCGTC CAGGCACTCC TCTCTCTTTC TCACCAGCTC CTCCACCACC AGGTGCTCGA 
     1501 TCTCGTCGCT TCTGAAGTCG TGCAGGTTCA CCAGCTTGTC GGGGGGGCAC CACTTGGTCT 
     1561 CGTTGCTGGT CTGCATGCTC ACCCAGGTGC CGTCCATCAG TCTCAGGCCC AGCACGCCGC 
     1621 ACAGCTTCAG CTTGCAGGCG CCCTTCAGGC TCTTGTACAG GCCTCTCTCG TCCACGAAGC 
     1681 CGCAGGTCTC GCTGCCCTTG CTGGCTCTCT TGCCTCTGCT GTTGGTGAAG ATGTCGCAGC 
     1741 TCATGCCCAG TCTGGGGTTC TCGGGCATCC AGATGGTGTA GTCGTGGTTG GTGCTGCAGT 
     1801 AGGTGCTGCT CACGGCCACG CCGCTGCACT TGCCGCTGGG GAACACTCTG CTGTGCAGGC 
     1861 TTCTGTCGTA GGGGTCCAGG TCGGCCACGC TGGGGCTGAT GATCACCAGG CTCTCCTTGG 
     1921 TGGTCTTCAC GGTTCTCAGC CATCTGTAGT CGGGGTAGGG GTTGTGCAGG CTCTCCTCGT 
     1981 ATCTGGGGTC GCCGGCCATC TTCCAGTTGT AGGCGGCTCT GCAGGCGTCG GGGGTGGGTC 
     2041 TGAAGTGCTT TCTCTTGAAG GTGGTGGTCA CGTAGCCCAC GAAGTTGGTG TAGGTCTCGG 
     2101 CCTCGGTCAC CACGCCGGTG CAGGTGAAGC CGTTCACCTT GATGGCCAGG ATGTAGCCCA 
     2161 CCTTCAGCTC CATGTAGCTG AAGCCGCTCA GGTTGGTGCA GCCCTCGTCC TCCACCACCA 
     2221 GGTTGTTGGG GCAGCTCAGG TGGTGGATGT CGATGGGGCT CCAGGGGCCC AGCTTGTCGG 
     2281 GGATGGTGTA GATGGGGAAC TTGCCGAAGC ACAGGGGGAA CACCAGCAGG GGCACGAACA 
     2341 GCAGGGCCTG GGGCACTCCG GAAGGACCGG GGTTACTTTC AACATCGCCA GCGAGTTTCA 
     2401 ACAAAGCGTA GTTAGTACAT TGACCACTAC CGCTAGCctt gtacagctcg tccatgccga 
     2461 gagtgatccc ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg 
     2521 ggtctttgct cagcttggac tgggtgctca ggtagtggtt gtcgggcagc agcacggggc 
     2581 cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag ctgcacgctg ccgtcctcga 
     2641 tgttgtggcg gatcttgaag ttggccttga tgccgttctt ctgcttgtcg gcggtgatat 
     2701 agacgttgtc gctgatggcg ttgtactcca gcttgtgccc caggatgttg ccgtcctcct 
     2761 tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg aacttcacct 
     2821 cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc tggacgtagc 
     2881 cttcgggcat ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg tagcgggcga 
     2941 agcactgcac gccccaggtc agggtggtca cgagggtggg ccagggcacg ggcagcttgc 
     3001 cggtggtgca gatgaacttc agggtcagct tgccgtaggt ggcatcgccc tcgccctcgc 
     3061 cggacacgct gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg atgggCACCA 
     3121 CCCCGGTGAA CAGCTCCTCG CCCTTGCTCA CCTCGACGCA TAACTTCGTA TAGGATACTT 
     3181 TATACGAAGT TATCTTTGGG ATTCTTCCTA TTTTGATCCA AGCATCACCA TCGACCCTCT 
     3241 AGTCCAGATC TCACCATCGA CCCATAACTT CGTATAGCAT ACATTATACG AAGTTATGTC 
     3301 CCTCGAAGAG GTTCGaattC CCCGGGGATC CTCTAGAGTC GACCTGCAGA AGCTTGCCTC 
     3361 GAGCAGCGCT GCTCGAGAGA TCTACGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT 
     3421 CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG 
     3481 CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG 
     3541 GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG 
     3601 CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT 
     3661 TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA tgcaTGACCA GGCTCAGCTA 
     3721 ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC 
     3781 CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC 
     3841 CACTGCTCCC TTCCCTGTCC TTCTGATTTT GTAGGTAACC ACGTGCGGAC CGAGCGGCCG 
     3901 CAGGAACCCC TAGTGATGGA GTTGGCCACT CCCTCTCTGC GCGCTCGCTC GCTCACTGAG 
     3961 GCCGGGCGAC CAAAGGTCGC CCGACGCCCG GGCTTTGCCC GGGCGGCCTC AGTGAGCGAG 
     4021 CGAGCGCGCA GCTGCCTGCA GGGGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC 
     4081 GGTATTTCAC ACCGCATACG TCAAAGCAAC CATAGTACGC GCCCTGTAGC GGCGCATTAA 
     4141 GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC 
     4201 CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG 
     4261 CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA 
     4321 AAAAACTTGA TTTGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC 
     4381 GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA 
     4441 CACTCAACCC TATCTCGGGC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT 
     4501 ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTTAAC AAAATATTAA 
     4561 CGTTTACAAT TTTATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC 
     4621 AGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT 
     4681 CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT 
     4741 CATCACCGAA ACGCGCGAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG 
     4801 TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA 
     4861 CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC 
     4921 CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG 
     4981 TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC 
     5041 TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG 
     5101 ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA 
     5161 GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC 
     5221 AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG 
     5281 AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA 
     5341 GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG 
     5401 CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA 
     5461 ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT 
     5521 TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT 
     5581 GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT 
     5641 TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG 
     5701 GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA 
     5761 TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC 
     5821 TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA 
     5881 AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT 
     5941 TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT 
     6001 TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT 
     6061 GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC 
     6121 AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG 
     6181 TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG 
     6241 ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT 
     6301 CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC 
     6361 TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG 
     6421 ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG 
     6481 GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT 
     6541 TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT 
     6601 TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCAcatg
//