LOCUS       _pCMV-pSNAPm_NEU        8198 bp    DNA     circular     14-JAN-2009
SOURCE      
  ORGANISM  
COMMENT     This vector has been fully sequenced.
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|507205641|
COMMENT     VNTDBDATE|507205641|
COMMENT     LSOWNER|
COMMENT     VNTNAME|_pCMV-pSNAPm_NEU|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|Invitrogen|
COMMENT     VNTOAUTHORTEL|(800) 955-6288|
COMMENT     VNTOAUTHOREML|bioinfosales@invitrogen.com|
COMMENT     VNTOAUTHORAD1|Invitrogen Corporation|
COMMENT     VNTOAUTHORAD2|5791 Van Allen Way|
COMMENT     VNTOAUTHORAD3|Carlsbad, CA 92008 U.S.A.|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
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COMMENT        #6=(CRSite (CStringList) "PstI" "ctgcag" 6 0 5 938 0 1213 0 1351 0 2141 
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COMMENT        #10=(CRSite (CStringList) "BglII" "agatct" 2 0 2 13 0 1307 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "NruI" "tcgcga" 4 0 1 209 0 0 "") 
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COMMENT             "") 
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COMMENT             "")) 
COMMENT       (CObList 
COMMENT        #23=(CFSignal (CObList) "CMV promoter (209-862)" 29 0 0 209 861 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "209..861") 
COMMENT        #24=(CFSignal (CObList) "Flag-Tag (901-927)" 21 0 0 901 926 0 
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COMMENT        #27=(CFSignal (CObList) "NeoR" 88 0 0 4903 5696 0 (CStringList) 
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COMMENT        #28=(CFSignal (CObList) "AmpR" 21 0 1 7202 7642 0 (CStringList) 
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COMMENT                          "Currently local object. Original author: Invitrogen" 
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COMMENT                          "Created: 01/14/09 10:27" 1) 
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COMMENT                          "Promoter Eukaryotic (1 total)") 
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FEATURES             Location/Qualifiers
     promoter        209..861
                     /vntifkey="29"
                     /label=CMV\promoter\(209-862)
     misc_feature    901..926
                     /vntifkey="21"
                     /label=Flag-Tag\(901-927)
     exon            2346..3019
                     /vntifkey="61"
                     /label=EosFP_1_cDNA\(967-1641)
     exon            3063..3739
                     /vntifkey="61"
                     /label=EosFP_2_cDNA\(1684-2361)
     misc_structure  4903..5696
                     /vntifkey="88"
                     /label=NeoR
     misc_feature    complement(7202..7642)
                     /vntifkey="21"
                     /label=AmpR
     misc_feature    3021..3062
                     /vntifkey="21"
                     /label=Flexible\Linker\(1642-1683)
     misc_feature    953..2345
                     /vntifkey="21"
                     /label=TNFR1
BASE COUNT     1948 a      2126 c      2113 g      2011 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc 
      901 atggactaca aagacgatga cgataaagaa ttctgcagat atccatcaca ctgcggccgc 
      961 tgtctggcat gggcctctcc accgtgcctg acctgctgct gccactggtg ctcctggagc 
     1021 tgttggtggg aatatacccc tcaggggtta ttggactggt ccctcaccta ggggacaggg 
     1081 agaagagaga tagtgtgtgt ccccaaggaa aatatatcca ccctcaaaat aattcgattt 
     1141 gctgtaccaa gtgccacaaa ggaacctact tgtacaatga ctgtccaggc ccggggcagg 
     1201 atacggactg cagggagtgt gagagcggct ccttcaccgc ttcagaaaac cacctcagac 
     1261 actgcctcag ctgctccaaa tgccgaaagg aaatgggtca ggtggagatc tcttcttgca 
     1321 cagtggaccg ggacaccgtg tgtggctgca ggaagaacca gtaccggcat tattggagtg 
     1381 aaaacctttt ccagtgcttc aattgcagcc tctgcctcaa tgggaccgtg cacctctcct 
     1441 gccaggagaa acagaacacc gtgtgcacct gccatgcagg tttctttcta agagaaaacg 
     1501 agtgtgtctc ctgtagtaac tgtaagaaaa gcctggagtg cacgaagttg tgcctacccc 
     1561 agattgagaa tgttaagggc actgaggact caggcaccac agtgctgttg cccctggtca 
     1621 ttttctttgg tctttgcctt ttatccctcc tcttcattgg tttaatgtat cgctaccaac 
     1681 ggtggaagtc caagctctac tccattgttt gtgggaaatc gacacctgaa aaagaggggg 
     1741 agcttgaagg aactactact aagcccctgg ccccaaaccc aagcttcagt cccactccag 
     1801 gcttcacccc caccctgggc ttcagtcccg tgcccagttc caccttcacc tccagctcca 
     1861 cctatacccc cggtgactgt cccaactttg cggctccccg cagagaggtg gcaccaccct 
     1921 atcagggggc tgaccccatc cttgcgacag ccctcgcctc cgaccccatc cccaaccccc 
     1981 ttcagaagtg ggaggacagc gcccacaagc cacagagcct agacactgat gaccccgcga 
     2041 cgctgtacgc cgtggtggag aacgtgcccc cgttgcgctg gaaggaattc gtgcggcgcc 
     2101 tagggctgag cgaccacgag atcgatcggc tggagctgca gaacgggcgc tgcctgcgcg 
     2161 aggcgcaata cagcatgctg gcgacctgga ggcggcgcac gccgcggcgc gaggccacgc 
     2221 tggagctgct gggacgcgtg ctccgcgaca tggacctgct gggctgcctg gaggacatcg 
     2281 aggaggcgct ttgcggcccc gccgccctcc cgcccgcgcc cagtcttctc agatttggcc 
     2341 tcgagagtgc gattaagcca gacatgaaga tcaacctccg tatggaaggc aacgtaaacg 
     2401 ggcaccactt tgtgatcgac ggagatggta caggcaagcc ttttgaggga aaacagagta 
     2461 tggatcttga agtcaaagag ggcggacctc tgccttttgc ctttgatatc ctgaccactg 
     2521 cattccatta cggcaacagg gtattcgccg aatatccaga ccacatacaa gactatttta 
     2581 agcagtcgtt tcctaagggg tattcgtggg aacgaagctt gactttcgaa gacgggggca 
     2641 tttgcattgc cagaaacgac ataacaatgg aaggggacac tttctataat aaagttcgat 
     2701 ttcacggtgt aaactttccc gccaatggtc cagttatgca gaagaagacg ctgaaatggg 
     2761 agccctccac tgagaaaatg tatgtgcgtg atggagtgct gaccggtgat attcgcatgg 
     2821 ctttgttgct tgaaggaaat gcccattacc gatgtgactt cagaactact tacaaagcta 
     2881 aggagaaggg tgtcaagtta ccaggctacc actttgtgga ccactgcatt gagattttaa 
     2941 gccatgacaa agattacaac aaggttaagc tgtatgagca tgctgttgct cattctggat 
     3001 tgcctgacaa tgccagacga tctagaggtc acggtactgg ttctactggt tctggttctt 
     3061 ctagtgcgat taagccagac atgaagatca acctccgtat ggaaggcaac gtaaacgggc 
     3121 accactttgt gatcgacgga gatggtacag gcaagccttt tgagggaaaa cagagtatgg 
     3181 atcttgaagt caaagagggc ggacctctgc cttttgcctt tgatatcctg accactgcat 
     3241 tccattacgg caacagggta ttcgccgaat atccagacca catacaagac tattttaagc 
     3301 agtcgtttcc taaggggtat tcgtgggaac gaagcttgac tttcgaagac gggggcattt 
     3361 gcattgccag aaacgacata acaatggaag gggacacttt ctataataaa gttcgatttc 
     3421 acggtgtaaa ctttcccgcc aatggtccag ttatgcagaa gaagacgctg aaatgggagc 
     3481 cctccactga gaaaatgtat gtgcgtgatg gagtgctgac cggtgatatt cgcatggctt 
     3541 tgttgcttga aggaaatgcc cattaccgat gtgacttcag aactacttac aaagctaagg 
     3601 agaagggtgt caagttacca ggctaccact ttgtggacca ctgcattgag attttaagcc 
     3661 atgacaaaga ttacaacaag gttaagctgt atgagcatgc tgttgctcat tctggattgc 
     3721 ctgacaatgc cagacgataa gggccctatt ctatagtgtc acctaaatgc tagagctcgc 
     3781 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg 
     3841 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt 
     3901 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc 
     3961 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct 
     4021 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc 
     4081 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc 
     4141 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc 
     4201 cgtcaagctc taaatcgggg catcccttta gggttccgat ttagtgcttt acggcacctc 
     4261 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg 
     4321 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact 
     4381 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttggggatt 
     4441 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt 
     4501 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccaggcagg cagaagtatg 
     4561 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca 
     4621 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact 
     4681 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta 
     4741 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag 
     4801 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc 
     4861 attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga 
     4921 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa 
     4981 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt 
     5041 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg 
     5101 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa 
     5161 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac 
     5221 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt 
     5281 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact 
     5341 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg 
     5401 ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg 
     5461 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc 
     5521 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt 
     5581 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc 
     5641 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg 
     5701 ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg 
     5761 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct 
     5821 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta 
     5881 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat 
     5941 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct 
     6001 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt 
     6061 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag 
     6121 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt 
     6181 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 
     6241 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 
     6301 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 
     6361 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 
     6421 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 
     6481 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 
     6541 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 
     6601 ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca 
     6661 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 
     6721 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 
     6781 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 
     6841 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct 
     6901 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 
     6961 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 
     7021 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 
     7081 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 
     7141 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 
     7201 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 
     7261 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 
     7321 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 
     7381 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 
     7441 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 
     7501 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 
     7561 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 
     7621 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 
     7681 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 
     7741 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 
     7801 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc 
     7861 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 
     7921 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 
     7981 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 
     8041 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 
     8101 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 
     8161 ttccgcgcac atttccccga aaagtgccac ctgacgtc 
//
