LOCUS       Exported                5167 bp ds-DNA     circular SYN 05-JUN-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    Ypet FtnA plasmidmap
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jun 5, 2017 from SnapGene 3.1.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    10..563
                     /note="CMV immearly promoter"
     misc_feature    520..589
                     /note="CMV promoter"
     CDS             610..1376
                     /note="Ypet"
                     /note="A206K"
     misc_feature    1225..1227
                     /note="A206k Mutation"
     gene            1324..1941
                     /note="FtnA"
     misc_feature    2700..2916
                     /note="SV40 origin"
     misc_feature    2712..2981
                     /note="SV40 promoter"
     misc_feature    3066..3854
                     /note="NeoR"
     misc_feature    4449..5069
                     /note="pBR322 origin"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gccaccggtc
      601 gccaccatgg tgagcaaagg cgaagagctg ttcaccggcg tggtgcccat cctggtggag
      661 ctggacggcg acgtgaacgg ccacaagttc agcgtgagcg gcgagggcga gggcgacgcc
      721 acctacggca agctgaccct gaagctgctg tgcaccaccg gcaagctgcc cgtgccctgg
      781 cccaccctgg tgaccaccct gggctacggc gtgcagtgct tcgcccggta ccccgaccac
      841 atgaagcagc acgacttctt caagagcgcc atgcccgagg gctacgtgca ggagcggacc
      901 atcttcttca aggacgacgg caactacaag acccgggccg aggtgaagtt cgagggcgac
      961 accctggtga accggatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     1021 ggccacaagc tggagtacaa ctacaacagc cacaacgtgt acatcaccgc cgacaagcag
     1081 aagaacggca tcaaggccaa cttcaagatc cggcacaaca tcgaggacgg cggcgtgcag
     1141 ctggccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     1201 aaccactacc tgagctacca gagcgccctg ttcaaggacc ccaacgagaa gcgggaccac
     1261 atggtgctgc tggagttcct gaccgccgcc ggcatcaccg agggcatgaa cgagctctat
     1321 aaggctagcg gagcatccgc aggaatgctg aaaccagaaa tgattgaaaa acttaatgag
     1381 cagatgaacc tggaactgta ctcttcactg ctttatcagc aaatgagcgc ctggtgcagc
     1441 tatcatacct tcgaaggtgc tgccgcgttc ctgcgccgtc acgcccagga agagatgacg
     1501 catatgcagc gtctgtttga ttacctgact gataccggca atttaccgcg tattaatacc
     1561 gttgaatctc cgtttgctga atattcctca cttgatgaat tattccagga aacctataaa
     1621 cacgaacaat taatcaccca gaaaattaac gaactggctc atgctgcaat gaccaatcag
     1681 gactacccaa catttaattt cctgcaatgg tatgtttctg agcagcatga agaagagaaa
     1741 ctgttcaaat cgattattga taaattaagc ctggcaggca aaagcggcga aggtctgtat
     1801 tttatcgaca aagaactctc taccctcgac acacaaaact aacggccgca tcgataacag
     1861 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     1921 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     1981 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     2041 tagttgtggt ttgtccaaac tcatcaatgt atcttaacgc gtaaattgta agcgttaata
     2101 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     2161 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     2221 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     2281 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     2341 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     2401 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     2461 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     2521 cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     2581 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     2641 tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt
     2701 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     2761 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     2821 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     2881 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     2941 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     3001 cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc
     3061 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     3121 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     3181 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     3241 tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     3301 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     3361 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     3421 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     3481 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     3541 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg
     3601 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     3661 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     3721 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     3781 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     3841 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
     3901 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     3961 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     4021 cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg
     4081 cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa
     4141 acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg
     4201 ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc
     4261 agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat
     4321 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     4381 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     4441 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     4501 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     4561 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     4621 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     4681 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     4741 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     4801 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     4861 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     4921 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     4981 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     5041 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     5101 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     5161 catgcat
//
