LOCUS       Exported                6800 bp ds-DNA     circular SYN 05-JUN-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pIRESpuro2-CD86-mEOS2.txt
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jun 5, 2017 from SnapGene 3.1.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6800
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    309..786
                     /note="CMV\IE\Promoter/Enhancer"
     misc_feature    871..2589
                     /note="CD86-mEOS2"
     misc_feature    871..1840
                     /note="CD86"
     misc_feature    2591..2919
                     /note="IVS"
     intron          2623..2918
                     /note="synth int"
     misc_feature    3552..4149
                     /note="Puro(R)"
                     /note="label=(Puro(R) - 3552..2555"
     terminator      4290..4517
                     /note="bGH PA"
     primer_bind     complement(4639..4659)
                     /note="M13-rev"
     misc_binding    complement(4665..4687)
                     /note="LacO"
     promoter        complement(4692..4721)
                     /note="lac"
     rep_origin      complement(5027..5655)
                     /direction=LEFT
                     /note="ColE1 origin"
     misc_feature    5040..5640
                     /note="pUC\Origin"
     CDS             complement(5807..6466)
                     /note="AmpR"
     promoter        complement(6706..6734)
                     /note="Amp prom"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc
      841 gctagctaat ctcgagatgc tagcgccacc atgggactga gtaacattct ctttgtgatg
      901 gccttcctgc tctctggtgc tgctcctctg aagattcaag cttatttcaa tgagactgca
      961 gacctgccat gccaatttgc aaactctcaa aaccaaagcc tgagtgagct agtagtattt
     1021 tggcaggacc aggaaaactt ggttctgaat gaggtatact taggcaaaga gaaatttgac
     1081 agtgttcatt ccaagtatat gggccgcaca agttttgatt cggacagttg gaccctgaga
     1141 cttcacaatc ttcagatcaa ggacaagggc ttgtatcaat gtatcatcca tcacaaaaag
     1201 cccacaggaa tgattcgcat ccaccagatg aattctgaac tgtcagtgct tgctaacttc
     1261 agtcaacctg aaatagtacc aatttctaat ataacagaaa atgtgtacat aaatttgacc
     1321 tgctcatcta tacacggtta cccagaacct aagaagatga gtgttttgct aagaaccaag
     1381 aattcaacta tcgagtatga tggtattatg cagaaatctc aagataatgt cacagaactg
     1441 tacgacgttt ccatcagctt gtctgtttca ttccctgatg ttacgagcaa tatgaccatc
     1501 ttctgtattc tggaaactga caagacgcgg cttttatctt cacctttctc tatagagctt
     1561 gaggaccctc agcctccccc agaccacatt ccttggatta cagctgtact tccaacagtt
     1621 attatatgtg tgatggtttt ctgtctaatt ctatggaaat ggaagaagaa gaagcggcct
     1681 cgcaactctt ataaatgtgg aaccaacaca atggagaggg aagagagtga acagaccaag
     1741 aaaagagaaa aaatccatat acctgaaaga tctgatgaaa cccagcgtgt ttttaaaagt
     1801 tcgaagacat cttcatgcga caaaagtgat acatgttttg gaggcggacc ggtgccccag
     1861 tgggagggat tcgctgcgct gctggcaacc ccggtcggag gcgctgtcat gtctgctatc
     1921 aagccagaca tgaagatcaa gttgagaatg gaaggtaacg ttaacggtca ccacttcgtt
     1981 atcgacggtg acggtactgg taagccattc gaaggtaagc aatctatgga cttggaagtt
     2041 aaggaaggtg gtccattgcc attcgctttc gacatcttga ctactgcttt ccactacggt
     2101 aacagagttt tcgctaagta cccagacaac atccaagact acttcaagca atctttccca
     2161 aagggttact cttgggaaag atctttgact ttcgaagacg gtggtatctg tatcgctaga
     2221 aacgacatca ctatggaagg tgacactttc tacaacaagg ttagattcta cggtactaac
     2281 ttcccagcta acggtccagt tatgcaaaag aagactttga agtgggaacc atctactgaa
     2341 aagatgtacg ttagagacgg tgttttgact ggtgacatcc acatggcttt gttgttggaa
     2401 ggtaacgctc actacagatg tgacttcaga actacttaca aggctaagga aaagggtgtt
     2461 aagttgccag gttaccactt cgttgaccac tgtatcgaaa tcttgtctca cgacaaggac
     2521 tacaacaagg ttaagttgta cgaacacgct gttgctcact ctggtttgcc agacaacgct
     2581 agaagataaa gcggccgcat agataactga tccagtgtgc tggaattaat tcgctgtctg
     2641 cgagggccag ctgttggggt gagtactccc tctcaaaagc gggcatgact tctgcgctaa
     2701 gattgtcagt ttccaaaaac gaggaggatt tgatattcac ctggcccgcg gtgatgcctt
     2761 tgagggtggc cgcgtccatc tggtcagaaa agacaatctt tttgttgtca agcttgaggt
     2821 gtggcaggct tgagatctgg ccatacactt gagtgacaat gacatccact ttgcctttct
     2881 ctccacaggt gtccactccc aggtccaact gcaggtcgag catgcatcta gggcggccaa
     2941 ttccgcccct ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc
     3001 cggtgtgcgt ttgtctatat gtgattttcc accatattgc cgtcttttgg caatgtgagg
     3061 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
     3121 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
     3181 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
     3241 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
     3301 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc
     3361 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
     3421 cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac
     3481 cacggggacg tggttttcct ttgaaaaaca cgatgataag cttgccacaa cccacaagga
     3541 gacgaccttc catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     3601 cccgggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     3661 tcgacccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
     3721 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
     3781 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
     3841 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     3901 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     3961 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     4021 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     4081 ccgtcaccgc cgacgtcgag tgcccgaagg accgcgcgac ctggtgcatg acccgcaagc
     4141 ccggtgcctg acgcccgccc cacgacccgc agcgcccgac cgaaaggagc gcacgacccc
     4201 atggctccga ccgaagccga cccgggcggc cccgccgacc ccgcacccgc ccccgaggcc
     4261 caccgactct agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     4321 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     4381 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     4441 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     4501 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcga gtgcattcta
     4561 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
     4621 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     4681 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     4741 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     4801 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     4861 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     4921 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     4981 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     5041 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     5101 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     5161 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     5221 gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc
     5281 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     5341 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     5401 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     5461 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     5521 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     5581 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     5641 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     5701 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     5761 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     5821 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     5881 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     5941 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     6001 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     6061 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     6121 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     6181 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     6241 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     6301 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     6361 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     6421 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     6481 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     6541 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     6601 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     6661 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     6721 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     6781 gaaaagtgcc acctgacgtc
//
