LOCUS       pPMVAu5                12354 bp    DNA     circular SYN 29-NOV-2013
DEFINITION  
ACCESSION   pPMVAu5
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 12354)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
FEATURES             Location/Qualifiers
     promoter        10..650
                     /gene="SSA1p"
     CDS             651..1845
                     /gene="ERG10"
     terminator      1854..2153
                     /gene="NAT1t"
     promoter        2160..2809
                     /gene="RPL4Ap"
     CDS             2810..4283
                     /gene="ERG13"
     terminator      4292..4591
                     /gene="EFM1t"
     promoter        4598..5220
                     /gene="RPL15Ap"
     CDS             5221..6796
                     /gene="tHMG1"
     terminator      6805..7219
                     /gene="EBS1t"
     rep_origin      7660..8279
                     /gene="ori"
     CDS             8434..9277
                     /gene="bla"
     rep_origin      9426..10397
                     /gene="2micron"
     CDS             11339..11995
                     /gene="HIS3"
ORIGIN      
        1 taaggatcca tagtgggtag ataaccttaa actatttatt tagaggcgac aatgtgaaaa
       61 attttttctt caaaggctcg gttgtcgaca aattgttacg ttgtgctttg atttctaaag
      121 cgcttcttca cctgcaggtt ctgagcccta agaaaaaaaa tttccttggt tgaaaatggc
      181 ggaaaaaaaa aattcagaaa aagaaataaa gcacgtgtgc gcggtgtgtg gatgatggtt
      241 tcatcattgt caacggcatt ttcgttcttg tggattgttg taaactttcc agaacattct
      301 agaaagaaag cacacggaac gtttagaagc tgtcatttgc gttttttctc cagattttag
      361 ttgagaaagt aattaaatta ttcttctttt tccagaacgt tccatcggcg gcaaaaggga
      421 gagaaagaac ccaaaaagaa ggggggccat ttagattagc tgatcgtttc gaggacttca
      481 aggttatata aggggtggat tgatgtatct tcgagaaggg attgagttgt agtttcgttt
      541 cccaattctt acttaagttg ttttattttc tctatttgta agataagcac atcaaaagaa
      601 aagtaatcaa gtattacaag aaacaaaaat tcaagtaaat aacagataaa atgtctcaga
      661 acgtttacat tgtatcgact gccagaaccc caattggttc attccagggt tctctatcct
      721 ccaagacagc agtggaattg ggtgctgttg ctttaaaagg cgccttggct aaggttccag
      781 aattggatgc atccaaggat tttgacgaaa ttatttttgg taacgttctt tctgccaatt
      841 tgggccaagc tccggccaga caagttgctt tggctgccgg tttgagtaat catatcgttg
      901 caagcacagt taacaaggtc tgtgcatccg ctatgaaggc aatcattttg ggtgctcaat
      961 ccatcaaatg tggtaatgct gatgttgtcg tagctggtgg ttgtgaatct atgactaacg
     1021 caccatacta catgccagca gcccgtgcgg gtgccaaatt tggccaaact gttcttgttg
     1081 atggtgtcga aagagatggg ttgaacgatg cgtacgatgg tctagccatg ggtgtacacg
     1141 cagaaaagtg tgcccgtgat tgggatatta ctagagaaca acaagacaat tttgccatcg
     1201 aatcctacca aaaatctcaa aaatctcaaa aggaaggtaa attcgacaat gaaattgtac
     1261 ctgttaccat taagggattt agaggtaagc ctgatactca agtcacgaag gacgaggaac
     1321 ctgctagatt acacgttgaa aaattgagat ctgcaaggac tgttttccaa aaagaaaacg
     1381 gtactgttac tgccgctaac gcttctccaa tcaacgatgg tgctgcagcc gtcatcttgg
     1441 tttccgaaaa agttttgaag gaaaagaatt tgaagccttt ggctattatc aaaggttggg
     1501 gtgaggccgc tcatcaacca gctgatttta catgggctcc atctcttgca gttccaaagg
     1561 ctttgaaaca tgctggcatc gaagacatca attctgttga ttactttgaa ttcaatgaag
     1621 ccttttcggt tgtcggtttg gtgaacacta agattttgaa gctagaccca tctaaggtta
     1681 atgtatatgg tggtgctgtt gctctaggtc acccattggg ttgttctggt gctagagtgg
     1741 ttgttacact gctatccatc ttacagcaag aaggaggtaa gatcggtgtt gccgccattt
     1801 gtaatggtgg tggtggtgct tcctctattg tcattgaaaa gatataatga taacctgcaa
     1861 ctcctcaatg tgtcaattaa ctcttactta atttatgtat atatttttta tgtatatgct
     1921 tatatgcatg cgcatatgct cataaaagat acattgttat aggtcatttc ttttccaagc
     1981 tacatctagc actccttcca accatggtcc aggacagcca ggaccttttc ttgccaaaat
     2041 tcttgctttc ccatctcaga gaaaaaacga ggaatttttc tttgtcctct ttggccgcca
     2101 gttgcggtaa aatatttttt ttctaggtcg catagtttga aagacttgtc tttactagta
     2161 aatcagacaa tctgtctgac gatatagaga gcgaagagga agaggaagag gaagagggtg
     2221 atgatagctt ggaatcatgg ctttcccagc tttacataga ctcaagtggc gagccttcac
     2281 cctcaacgtg ggccttagct aaccttttga ctttaacggc cgttcaatgg gaatcattgt
     2341 tttccaaaaa ggccactcct cacatcagtg attctatagt taacgaagaa aagctgccct
     2401 tcctggccaa aaaagacaat ccccattcca agaaactgct ttccaactta cttaaggaaa
     2461 agcaactacc ttgcataaaa ggcgacaact cgtcaaagat aacaagtgct actaaaagca
     2521 tgctccaaaa tgctcgaaca cttgtacaat cggagcataa tatcttagat agatgtctca
     2581 agagactatc ctaaataata ttgaatatgc acttttacta tattaatatc acgtcacacg
     2641 acgcacagtg agaagtgaaa aatttttttt caatctgaaa aaaaaaaaaa aaaaaaaaaa
     2701 aaatttatat aaacgaatgg tatctccatc acatttcttt tagcctcgca acttgtactt
     2761 ttcatcactt ttcttgtaat ttagcaatat cccaagaaca atcatcaaaa tgaaactctc
     2821 aactaaactt tgttggtgtg gtattaaagg aagacttagg ccgcaaaagc aacaacaatt
     2881 acacaataca aacttgcaaa tgactgaact aaaaaaacaa aagaccgctg aacaaaaaac
     2941 cagacctcaa aatgtcggta ttaaaggtat ccaaatttac atcccaactc aatgtgtcaa
     3001 ccaatctgag ctagagaaat ttgatggcgt ttctcaaggt aaatacacaa ttggtctggg
     3061 ccaaaccaac atgtcttttg tcaatgacag agaagatatc tactcgatgt ccctaactgt
     3121 tttgtctaag ttgatcaaga gttacaacat cgacaccaac aaaattggta gattagaagt
     3181 cggtactgaa actctgattg acaagtccaa gtctgtcaag tctgtcttga tgcaattgtt
     3241 tggtgaaaac actgacgtcg aaggtattga cacgcttaat gcctgttacg gtggtaccaa
     3301 cgcgttgttc aactctttga actggattga atctaacgca tgggatggta gagacgccat
     3361 tgtagtttgc ggtgatattg ccatctacga taagggtgcc gcaagaccaa ccggtggtgc
     3421 cggtactgtt gctatgtgga tcggtcctga tgctccaatt gtatttgact ctgtaagagc
     3481 ttcttacatg gaacacgcct acgattttta caagccagat ttcaccagcg aatatcctta
     3541 cgtcgatggt catttttcat taacttgtta cgtcaaggct cttgatcaag tttacaagag
     3601 ttattccaag aaggctattt ctaaagggtt ggttagcgat cccgctggtt cggatgcttt
     3661 gaacgttttg aaatatttcg actacaacgt tttccatgtt ccaacctgta aattggtcac
     3721 aaaatcatac ggtagattac tatataacga tttcagagcc aatcctcaat tgttcccaga
     3781 agttgacgcc gaattagcta ctcgcgatta tgacgaatct ttaaccgata agaacattga
     3841 aaaaactttt gttaatgttg ctaagccatt ccacaaagag agagttgccc aatctttgat
     3901 tgttccaaca aacacaggta acatgtacac cgcatctgtt tatgccgcct ttgcatctct
     3961 attaaactat gttggatctg acgacttaca aggcaagcgt gttggtttat tttcttacgg
     4021 ttccggttta gctgcatctc tatattcttg caaaattgtt ggtgacgtcc aacatattat
     4081 caaggaatta gatattacta acaaattagc caagagaatc accgaaactc caaaggatta
     4141 cgaagctgcc atcgaattga gagaaaatgc ccatttgaag aagaacttca aacctcaagg
     4201 ttccattgag catttgcaaa gtggtgttta ctacttgacc aacatcgatg acaaatttag
     4261 aagatcttac gatgttaaaa aataatgata actttgatct gtagcctaag tataaaattc
     4321 tacgtatgta tatatttaca tgcaattttt tctttttcca attcatgtta atgttcttca
     4381 tcatttgata ataggccaat gatattatct actatcttct ccttttgagg agaagctgct
     4441 ttcacttcct cgttgctttt ctcttcgtat aaatacatga atttcgactt gtccagcatt
     4501 tttttatcaa tacaatgcat gtgcccatga agttctacct ccgacaaatc tttaacaaaa
     4561 tatgtcgtta tttgtgtcat gaatttcagt actcgaggta ttcaagatgt tcttatcaaa
     4621 gcacttattt tttcattata tttctatatt gatttaagga cggtacgcta ttttctttgt
     4681 accttttact atgcataaaa aaaggggaag tgtttaacaa tcgacactta cttaatatgt
     4741 tttgccgccc ttcataagag ggtgtttcta aaatttattg ggcaagaatg agatggactc
     4801 gcaccctaca tgacgtttaa atatttagtg ttaaggttca gaacatgcac caggtgcgac
     4861 atgtgttgcg attatcatga caatgtctct atccgagatg catttgtagt atcaattgat
     4921 gcgtattatg acatgattta catagcatac atcgtcaaac atgatattat attctttttt
     4981 tgataaatgt acggatttaa agctgtcgaa tatattttct gaaatttctt ggagctgacg
     5041 caaaattttc aaaggtgcta aaatttttca agatttctca cttttgcttg gtaacaaaga
     5101 atgatggcat tgcattttta ccaccggtac atttaactgc tatttctcac gtttctttcc
     5161 ctatccttaa gtaattcttt tacaatctaa gaaaaccacg atcaaacaaa taaatcaaca
     5221 atggaccaat tggtgaaaac tgaagtcacc aagaagtctt ttactgctcc tgtacaaaag
     5281 gcttctacac cagttttaac caataaaaca gtcatttctg gatcgaaagt caaaagttta
     5341 tcatctgcgc aatcgagctc atcaggacct tcatcatcta gtgaggaaga tgattcccgc
     5401 gatattgaaa gcttggataa gaaaatacgt cctttagaag aattagaagc attattaagt
     5461 agtggaaata caaaacaatt gaagaacaaa gaggtcgctg ccttggttat tcacggtaag
     5521 ttacctttgt acgctttgga gaaaaaatta ggtgatacta cgagagcggt tgcggtacgt
     5581 aggaaggctc tttcaatttt ggcagaagct cctgtattag catctgatcg tttaccatat
     5641 aaaaattatg actacgaccg cgtatttggc gcttgttgtg aaaatgttat aggttacatg
     5701 cctttgcccg ttggtgttat aggccccttg gttatcgatg gtacatctta tcatatacca
     5761 atggcaacta cagagggttg tttggtagct tctgccatgc gtggctgtaa ggcaatcaat
     5821 gctggcggtg gtgcaacaac tgttttaact aaggatggta tgacaagagg cccagtagtc
     5881 cgtttcccaa ctttgaaaag atctggtgcc tgtaagatat ggttagactc agaagaggga
     5941 caaaacgcaa ttaaaaaagc ttttaactct acatcaagat ttgcacgtct gcaacatatt
     6001 caaacttgtc tagcaggaga tttactcttc atgagattta gaacaactac tggtgacgca
     6061 atgggtatga atatgatttc taaaggtgtc gaatactcat taaagcaaat ggtagaagag
     6121 tatggctggg aagatatgga ggttgtctcc gtttctggta actactgtac cgacaaaaaa
     6181 ccagctgcca tcaactggat cgaaggtcgt ggtaagagtg tcgtcgcaga agctactatt
     6241 cctggtgatg ttgtcagaaa agtgttaaaa agtgatgttt ccgcattggt tgagttgaac
     6301 attgctaaga atttggttgg atctgcaatg gctgggtctg ttggtggatt taacgcacat
     6361 gcagctaatt tagtgacagc tgttttcttg gcattaggac aagatcctgc acaaaatgtt
     6421 gaaagttcca actgtataac attgatgaaa gaagtggacg gtgatttgag aatttccgta
     6481 tccatgccat ccatcgaagt aggtaccatc ggtggtggta ctgttctaga accacaaggt
     6541 gccatgttgg acttattagg tgtaagaggc ccgcatgcta ccgctcctgg taccaacgca
     6601 cgtcaattag caagaatagt tgcctgtgcc gtcttggcag gtgaattatc cttatgtgct
     6661 gccctagcag ccggccattt ggttcaaagt catatgaccc acaacaggaa acctgctgaa
     6721 ccaacaaaac ctaacaattt ggacgccact gatataaatc gtttgaaaga tgggtccgtc
     6781 acctgcatta aatcctaatg ataatattgt tcgtttttta tcatagtttt tcaaatcttc
     6841 atatgaatac taggtagaaa tttggcttat cagtacgaat gaattacagt ttgactgatt
     6901 tgtacacgag aattgacgta taacttcttt tgaaagtctt tgaaattaca tggtagtaca
     6961 tacatatata tatatatata tatatgttat attattgtta attaaatatt attatgcgta
     7021 ttttcctttt ctttattagt atagtattaa tgacagtaat aataataata atagtaacaa
     7081 tatctctttt tttttttcag tgagctttta tttttttttc attgctcttc ttttggcctc
     7141 ttttgttttt ttcttgattt cctccagttt catctgtttt ttctttggat cagatacaaa
     7201 atctggtttg aacgcgagct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt
     7261 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     7321 caacatagga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgaggtaact
     7381 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     7441 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     7501 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     7561 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     7621 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     7681 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     7741 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     7801 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     7861 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     7921 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     7981 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     8041 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     8101 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     8161 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     8221 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     8281 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     8341 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     8401 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     8461 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     8521 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     8581 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     8641 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     8701 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     8761 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     8821 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     8881 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     8941 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     9001 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     9061 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     9121 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     9181 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     9241 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     9301 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     9361 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     9421 acctgaacga agcatctgtg cttcattttg tagaacaaaa atgcaacgcg agagcgctaa
     9481 tttttcaaac aaagaatctg agctgcattt ttacagaaca gaaatgcaac gcgaaagcgc
     9541 tattttacca acgaagaatc tgtgcttcat ttttgtaaaa caaaaatgca acgcgagagc
     9601 gctaattttt caaacaaaga atctgagctg catttttaca gaacagaaat gcaacgcgag
     9661 agcgctattt taccaacaaa gaatctatac ttcttttttg ttctacaaaa atgcatcccg
     9721 agagcgctat ttttctaaca aagcatctta gattactttt tttctccttt gtgcgctcta
     9781 taatgcagtc tcttgataac tttttgcact gtaggtccgt taaggttaga agaaggctac
     9841 tttggtgtct attttctctt ccataaaaaa agcctgactc cacttcccgc gtttactgat
     9901 tactagcgaa gctgcgggtg cattttttca agataaaggc atccccgatt atattctata
     9961 ccgatgtgga ttgcgcatac tttgtgaaca gaaagtgata gcgttgatga ttcttcattg
    10021 gtcagaaaat tatgaacggt ttcttctatt ttgtctctat atactacgta taggaaatgt
    10081 ttacattttc gtattgtttt cgattcactc tatgaatagt tcttactaca atttttttgt
    10141 ctaaagagta atactagaga taaacataaa aaatgtagag gtcgagttta gatgcaagtt
    10201 caaggagcga aaggtggatg ggtaggttat atagggatat agcacagaga tatatagcaa
    10261 agagatactt ttgagcaatg tttgtggaag cggtattcgc aatattttag tagctcgtta
    10321 cagtccggtg cgtttttggt tttttgaaag tgcgtcttca gagcgctttt ggttttcaaa
    10381 agcgctctga agttcctata ctttctagag aataggaact tcggaatagg aacttcaaag
    10441 cgtttccgaa aacgagcgct tccgaaaatg caacgcgagc tgcgcacata cagctcactg
    10501 ttcacgtcgc acctatatct gcgtgttgcc tgtatatata tatacatgag aagaacggca
    10561 tagtgcgtgt ttatgcttaa atgcgtactt atatgcgtct atttatgtag gatgaaaggt
    10621 agtctagtac ctcctgtgat attatcccat tccatgcggg gtatcgtatg cttccttcag
    10681 cactaccctt tagctgttct atatgctgcc actcctcaat tggattagtc tcatccttca
    10741 atgctatcat ttcctttgat attggatcat ctaagaaacc attattatca tgacattaac
    10801 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
    10861 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
    10921 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
    10981 ctatgcggca tcagagcaga ttgtactgag agtgcaccat aaattcccgt tttaagagct
    11041 tggtgagcgc taggagtcac tgccaggtat cgtttgaaca cggcattagt cagggaagtc
    11101 ataacacagt cctttcccgc aattttcttt ttctattact cttggcctcc tctagtacac
    11161 tctatatttt tttatgcctc ggtaatgatt ttcatttttt tttttcccct agcggatgac
    11221 tctttttttt tcttagcgat tggcattatc acataatgaa ttatacatta tataaagtaa
    11281 tgtgatttct tcgaagaata tactaaaaaa tgagcaggca agataaacga aggcaaagat
    11341 gacagagcag aaagccctag taaagcgtat tacaaatgaa accaagattc agattgcgat
    11401 ctctttaaag ggtggtcccc tagcgataga gcactcgatc ttcccagaaa aagaggcaga
    11461 agcagtagca gaacaggcca cacaatcgca agtgattaac gtccacacag gtatagggtt
    11521 tctggaccat atgatacatg ctctggccaa gcattccggc tggtcgctaa tcgttgagtg
    11581 cattggtgac ttacacatag acgaccatca caccactgaa gactgcggga ttgctctcgg
    11641 tcaagctttt aaagaggccc tactggcgcg tggagtaaaa aggtttggat caggatttgc
    11701 gcctttggat gaggcacttt ccagagcggt ggtagatctt tcgaacaggc cgtacgcagt
    11761 tgtcgaactt ggtttgcaaa gggagaaagt aggagatctc tcttgcgaga tgatcccgca
    11821 ttttcttgaa agctttgcag aggctagcag aattaccctc cacgttgatt gtctgcgagg
    11881 caagaatgat catcaccgta gtgagagtgc gttcaaggct cttgcggttg ccataagaga
    11941 agccacctcg cccaatggta ccaacgatgt tccctccacc aaaggtgttc ttatgtagtg
    12001 acaccgatta tttaaagctg cagcatacga tatatataca tgtgtatata tgtataccta
    12061 tgaatgtcag taagtatgta tacgaacagt atgatactga agatgacaag gtaatgcatc
    12121 attctatacg tgtcattctg aacgaggcgc gctttccttt tttctttttg ctttttcttt
    12181 ttttttctct tgaactcgac ggatctatgc ggtgtgaaat accgcacaga tgcgtaagga
    12241 gaaaataccg catcaggaaa ttgtaaacgt taatattttg ttaaaattcg cgttaaattt
    12301 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc ctta
//
