LOCUS Exported 11358 bp ds-DNA circular SYN 17-FEB-2017 DEFINITION pMZ374 sequence 11384 bps. ACCESSION . VERSION . KEYWORDS SnapGene DNA SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11358) AUTHORS . TITLE Direct Submission JOURNAL Exported Friday, Jun 2, 2017 from SnapGene 3.3.4 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11358 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 75..322 /gene="S. cerevisiae CYC1" /label=CYC1 terminator /note="transcription terminator for CYC1" CDS complement(338..358) /codon_start=1 /product="nuclear localization signal of SV40 large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS complement(371..4474) /codon_start=1 /product="Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=Cas9 /note="generates RNA-guided double strand breaks in DNA" /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" promoter complement(4495..5236) /label=adh1 promoter /note="promoter for the Schizosaccharomyces pombe adh1 gene encoding alcohol dehydrogenase" promoter 5265..5369 /gene="bla" /label=AmpR promoter CDS 5370..6230 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 6401..6989 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 7277..7298 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 7313..7343 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7351..7367 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 7375..7391 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" misc_feature 7428..7784 /label=rrk1 gene -1 to -358 promoter 7428..7784 /label=rrk1 promoter /leader /note="promoter and leader RNA for the Schizosaccharomyces pombe rrk1 gene encoding the K-RNA component of RNase P (Jacobs et al., 2014)" misc_feature 7765..7784 /label=20bp misc_feature 7785..7792 /label=Not1 Cutting Site misc_feature 7793..7830 /label=sgRNA Cassette misc_feature 7793..7812 /label=20bp misc_RNA 7877..7928 /label=sTRSV HHRz /note="hammerhead ribozyme from the tobacco ringspot virus satellite RNA (Khvorova et al., 2003)" primer_bind complement(7978..7994) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" CDS complement(8635..9429) /codon_start=1 /gene="Schizosaccharomyces pombe ura4" /product="orotidine 5'-phosphate decarboxylase, required for uracil biosynthesis" /label=S. pombe ura4 /translation="MDARVFQSYSARAEGMKNPIAKELLALMEEKQSNLSVAVDLTKKS EILELVDKIGPYVCVIKTHIDVVEDFDQDMVEKLVALGKKHRFLIFEDRKFADIGNTVK LQYASGVYKIASWAHITNCHTVPGEGIIQGLKEVGLPLGRGLLLLAEMSSKGSLATGSY TEKTLEWFEKHTDFCFGFIAGRRFPNLQSDYITMSPGIGLDVKGDGLGQQYRTPEEVIV NCGSDIIIVGRGVYGAGRNPVVEAKRYREAGWKAYQQRLSQH" rep_origin 10039..10820 /label=ars1 /note="Schizosaccharomyces pombe autonomously replicating sequence ars1" ORIGIN 1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 61 cttagacggg gatcgcaaat taaagccttc gagcgtccca aaaccttctc aagcaaggtt 121 ttcagtataa tgttacatgc gtacacgcgt ctgtacagaa aaaaaagaaa aatttgaaat 181 ataaataacg ttcttaatac taacataact ataaaaaaat aaatagggac ctagacttca 241 ggttgtctaa ctccttcctt ttcggttaga gcggatgtgg ggggagggcg tgaatgtaag 301 cgtgacataa ctaattacat gactcgagaa gagatcacac cttcctcttc ttcttggggt 361 cagccctgct gtctccaccg agctgagaga ggtcgattct tgtttcatag agccccgtaa 421 ttgactgatg aatcagtgtg gcgtccagga cctcctttgt agaggtgtac cgctttctgt 481 ctatggtggt gtcgaagtac ttgaaggctg caggcgcgcc caagttggtc agagtaaaca 541 agtggataat gttttctgcc tgctccctga tgggcttatc cctgtgctta ttgtaagcag 601 aaagcacctt atcgaggtta gcgtcggcga ggatcactct tttggagaat tcgcttattt 661 gctcgatgat ctcatcaagg tagtgtttgt gttgttccac gaacagctgc ttctgctcat 721 tatcttcggg agaccctttg agcttttcat agtggctggc cagatacaag aaattaacgt 781 atttagaggg cagtgccagc tcgttacctt tctgcagctc gcccgcacta gcgagcattc 841 gtttccggcc gttttcaagc tcaaagagag agtacttggg aagcttaatg atgaggtctt 901 ttttgacctc tttatatcct ttcgcctcga gaaagtcgat ggggtttttt tcgaagcttg 961 atcgctccat gattgtgatg cccagcagtt ccttgacgct tttgagtttt ttagacttcc 1021 ctttctccac tttggccaca accagtacac tgtaagcgac tgtaggagaa tcgaatccgc 1081 cgtatttctt ggggtcccaa tcttttttgc gtgcgatcag cttgtcgctg ttccttttcg 1141 ggaggatact ttccttggag aagcctccgg tctgtacttc ggtcttttta acgatgttca 1201 cctgcggcat ggacaggacc ttccggactg tcgcgaaatc cctacccttg tcccacacga 1261 tttctcctgt ttctccgttt gtttcgataa gtggtcgctt ccgaatctct ccattggcca 1321 gtgtaatctc ggtcttgaaa aaattcataa tattgctgta aaagaagtac ttagcggtgg 1381 ccttgcctat ttcctgctca gactttgcga tcattttcct aacatcgtac actttatagt 1441 ctccgtaaac aaattcagat tcaagcttgg gatatttttt gataagtgca gtgcctacca 1501 ctgcattcag gtaggcatca tgcgcatggt ggtaattgtt gatctctctc accttataaa 1561 actgaaagtc ctttctgaaa tctgagacca gcttagactt cagagtaata actttcacct 1621 ctcgaatcag tttgtcattt tcatcgtact tggtgttcat gcgtgaatcg agaatttggg 1681 ccacgtgctt ggtgatctgg cgtgtctcaa caagctgcct tttgatgaag ccggctttat 1741 ccaactcaga caggccacct cgttcagcct tagtcagatt atcgaacttc cgttgtgtga 1801 tcagtttggc gttcagcagc tgccgccaat aatttttcat tttcttgaca acttcttctg 1861 aggggacgtt atcactcttc cctctatttt tatcggatct tgtcaacact ttattatcaa 1921 tagaatcatc tttgagaaaa gactggggca cgatatgatc cacgtcgtag tcggagagcc 1981 gattgatgtc cagttcctga tccacgtaca tgtccctgcc gttctgcagg tagtacaggt 2041 agagcttctc attctgaagc tgggtgtttt caactgggtg ttccttaagg atttgggacc 2101 ccagttcttt tataccctct tcaatcctct tcatcctttc cctactgttc ttctgtccct 2161 tctgggtagt ttggttctct cgggccatct cgataacgat attctcgggc ttatgccttc 2221 ccattacttt gacgagttca tccacgacct taacggtctg cagtattccc tttttgatag 2281 ctgggctacc tgcaagatta gcgatgtgct cgtgaagact gtccccctgg ccagaaactt 2341 gtgctttctg gatgtcctcc ttaaaggtga gagagtcatc atggatcaac tgcatgaagt 2401 tccggttggc aaatccatcg gacttaagaa aatccaggat tgtctttcca gactgcttat 2461 ctcggatccc attgatcagt tttcttgaca gccgccccca tcctgtatat cggcgcctct 2521 tgagctgttt catgactttg tcgtcgaaga gatgagcgta agttttcaag cgttcttcaa 2581 tcatctccct atcttcaaac aacgtaaggg tgaggacaat gtcctcaaga atgtcctcgt 2641 tctcctcatt gtccaggaag tccttgtctt taatgatttt caggagatcg tgatacgttc 2701 ccagggatgc gttgaagcga tcctccactc cgctgatttc aacagagtcg aaacattcaa 2761 tctttttgaa atagtcttct ttgagctgtt tcacggtaac tttccggttc gtcttgaaga 2821 ggaggtccac gatagctttc ttctgctctc cagacaggaa tgctggcttt ctcatccctt 2881 ctgtgacgta tttgaccttg gtgagctcgt tataaactgt gaagtactcg tacagcagag 2941 agtgtttagg aagcaccttt tcgttaggca gatttttatc aaagttagtc atcctttcga 3001 tgaaggactg ggcagaggcc cccttatcca cgacttcctc gaagttccag ggagtgatgg 3061 tctcttctga tttgcgagtc atccacgcga atctggaatt tccccgggcg agggggccta 3121 catagtaggg tatccgaaat gtgaggattt tctcaatctt ttccctgtta tctttcaaaa 3181 aggggtagaa atcctcttgc cgcctgagga tagcgtgcag ttcgcccagg tgaatctggt 3241 gggggatgct tccattgtcg aaagtgcgct gtttgcgcaa cagatcttct ctgttaagct 3301 ttaccagcag ctcctcggtg ccgtccattt tttccaagat gggcttaata aatttgtaaa 3361 attcctcctg gcttgctccg ccgtcaatgt atccggcgta gccattttta gactgatcga 3421 agaaaatttc cttgtacttc tcaggcagtt gctgtctgac aagggccttc agcaaagtca 3481 agtcttggtg gtgctcatca tagcgcttga tcatactagc gctcagcgga gctttggtga 3541 tctccgtgtt cactcgcaga atatcactca gcagaatggc gtctgacagg ttctttgccg 3601 ccaaaaaaag gtctgcgtac tggtcgccga tctgggccag cagattgtcg agatcatcat 3661 cgtaggtgtc tttgctcagt tgaagcttgg catcttcggc caggtcgaag ttagatttaa 3721 agttgggggt cagcccgagt gacagggcga taagattacc aaacaggccg ttcttcttct 3781 ccccagggag ctgtgcgatg aggttttcga gccgccggga tttggacagc ctagcgctca 3841 ggattgcttt ggcgtcaact ccggatgcgt tgatcgggtt ctcttcgaaa agctgattgt 3901 aagtctgaac cagttggata aagagtttgt cgacatcgct gttgtctggg ttcaggtccc 3961 cctcgatgag gaagtgtccc cgaaatttga tcatatgcgc cagcgcgaga tagatcaacc 4021 gcaagtcagc cttatcagta ctgtctacaa gcttcttcct cagatgatat atggttgggt 4081 acttttcatg gtacgccacc tcgtccacga tattgccaaa gattgggtgg cgctcgtgct 4141 ttttatcctc ctccaccaaa aaggactcct ccagcctatg gaagaaagag tcatccacct 4201 tagccatctc attactaaag atctcctgca ggtagcagat ccgattcttt ctgcgggtat 4261 atctgcgccg tgctgttctt ttgagccgcg tggcttcggc cgtctccccg gagtcgaaca 4321 ggagggcgcc aatgaggttc ttctttatgc tgtggcgatc ggtattgccc agaactttga 4381 attttttgct cggcaccttg tactcgtccg taatgacggc ccaaccgacg ctgtttgtgc 4441 cgatatcgag cccaatggag tacttcttgt ccatctctag agtcgacctg cagcaattct 4501 cttgcttaaa gaaaagcgaa ggcacctgtc caccacccac tatttatacc atgggaggcg 4561 tcaaaccgag tttttgcgaa aggattatcg cacctctccc caagtaacat cggcaaagca 4621 aaaacggagc ggaagaccaa tgagacgcgg aaggggagcg ccgaatgagg aaagcgagag 4681 agaaagaagt ggatggaatg attccccgcc aaagtggaca catttcggga atcttcagga 4741 agcatggatc accttatgga caagaagata atccggaagc caataggagg gcgacagggg 4801 gtcagcgata agcgtcggaa aaaactttat gatatggtaa atacgtagga ggttacggtg 4861 cagaggaggg gccggtagcg agtgatagcg agggaaagac gatcgctatc gggccgtaag 4921 gaggaaatgg gaggttgggg gaaggatgcg ttggaatgcg gagtagagaa attgaagaga 4981 gagagaggac acacctagag agaaagaaat ggatatagag aaagagaaaa aataagagga 5041 atacggatac gatggagtgc gttgaatgag agaaaagaaa agaaaaggaa tgataagaga 5101 aggaatgaaa attgttttgt tttgttttca attgctttct tttgttttct ttgtctttgt 5161 tcttccttcc cttgtcccga tgctgttctt ctcaccagct tccgcatgat agccattttc 5221 ttagttgttg tagggcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt 5281 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa 5341 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta 5401 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag 5461 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca 5521 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta 5581 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc 5641 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc 5701 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca 5761 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc 5821 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca 5881 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac 5941 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg 6001 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg 6061 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg 6121 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac 6181 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc 6241 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct 6301 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc 6361 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc 6421 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg 6481 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa 6541 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc 6601 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt 6661 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa 6721 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc 6781 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc 6841 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct 6901 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat 6961 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc 7021 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg 7081 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc 7141 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg 7201 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca 7261 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact 7321 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa 7381 acagctatga catgattacg aattcgagct cggtaccccg ggtacctttt gcttatgttg 7441 gtggtagttg gcatgcgtag actgatgact agtcagcaag gagcgtagaa cagtcacact 7501 cgttatatat gtgcttccaa gaaaactcaa gaatttacca ttagcaaaca cttttttgaa 7561 atgttagaca tttaaatgac gaaggcatat agaagctttg aataggtgtt gtaaagtgtt 7621 gatttatgtg acgctgaggg tgcgcatgaa aggaatgttg ggtcacgatt attaaacagt 7681 ttgctagctt ggacacttga gtattggaag ttgttgaatt ctaaaaaact ttcagttgat 7741 ttgaatagtt gctgttgcca aaaaacataa cctgtaccga agaagcggcc gcgttttaga 7801 gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa agtgagtggc 7861 accgagtcgg tggtgccctg tcaccggatg tgctttccgg tctgatgagt ccgtgaggac 7921 gaaacagggg taccgtgggg atcctctaga gtcgacctgc aggcatgcaa gcttggcact 7981 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct 8041 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc 8101 ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac 8161 gcatctgtgc ggtatttcac accgcatacc atcgcgatga gcttgtgata ttgacgaaac 8221 tttttgacat ctaatttatt ctgttccaac accaatgttt ataaccaagt tttatcttgt 8281 ttgtctacat ggtattttac attcatctac atacatcttt cattggcttt gtacatagtt 8341 atcattacaa gtctaaaaaa attcactctt ttcttattca atgtcaatcc aagagaaaag 8401 attgtggtaa tgttgtagga gcatgtttaa taaattacta tagcaaatta ctttttattc 8461 ccaaggtgtt tatctataat agttaatatt ttagtcgcta cataaaattt taccaaagag 8521 tacttgtata ctaattctaa atgccttctg acataaaacg cctaggaaaa caaacgcaaa 8581 caaggcatcg actttttcaa taaccaacca aaaaaatttt acattagtct ttttttaatg 8641 ctgagaaagt ctttgctgat atgccttcca accagcttct ctatatctct tggcttcgac 8701 aacaggatta cgaccagctc catagactcc acgaccaaca atgatgatat cgctaccgca 8761 gtttacaatc acttcttcag gagtacgata ttgctgtccc agcccgtctc ctttaacatc 8821 caagccgata ccaggggaca tagttatgta gtcgctttga aggttaggaa atcgacgacc 8881 agctataaag ccaaagcaaa aatcggtatg cttctcaaac cattctaagg ttttctctgt 8941 gtaggaacca gtagccaaag agcctttgga agacatttca gccaaaagca agagaccacg 9001 tcccaaaggt aaaccaactt ctttgaggcc ttgtataata ccctcgcctg gcactgtatg 9061 gcaatttgtg atatgagccc aagaagcaat tttgtacaca ccagatgcat attgtagctt 9121 gacggtattt ccaatgtctg cgaatttgcg atcctcaaag ataagaaaac gatgcttttt 9181 acctaaggcc accagttttt ctaccatatc ctggtcgaaa tcctcgacaa cgtcaatatg 9241 tgtcttgata acacagacat agggtccaat tttatctacc aattctaaga tttcggattt 9301 cttcgtcaaa tcgaccgcga ctgacaagtt gctttgcttt tcttccatca aagccaacaa 9361 ttccttggca atgggatttt tcatcccctc agctctagct gaatagcttt gaaatactct 9421 agcatccata actttgcttt taaaccttta atttcgatcc aagcaaaaaa gaggttcttg 9481 gtaggacaat acggtaagaa aacacgacat gtgcagagat gccgacgaag catagttaaa 9541 ctgggatggt aaaatcaatt aagaatttat aaagacaaaa ttgtataagt ctctaaaaca 9601 tcttaattat acctcacaga actatctaaa atatattcac aaagtgcaaa cattatcatg 9661 aaaaagaacc attttaattt aaagcaaggg cattaaggct tatttacaga atttcttact 9721 tttgtaaaga ttataaggct gattatcttt ttcaccatgc caaaaattac acaagataga 9781 atggatgttt gaaattaaac gtgagtatac aaacaaatac actaggtaaa tcgaaacatt 9841 tttttctcca ttaagtaaca aattcctatt tagagaaaga atgctgagta gattaaataa 9901 tctatacaaa cttttttaac acaaatgcat acatatagcc agtgggattt gtagctaagc 9961 tcatcgatgg cgagtctaac tccttaacca ctcggacata gtgacttatc tgacactcat 10021 tgtaaattaa tatatatata ggcattttgt ttagttaaag gtacttaagt aattagtata 10081 aacgaaccaa ttttataatc aggaagttaa gtgaatggta gcacatgtcg taaaaattgt 10141 gaatttttat tgaataatat tttaaataca agcctttcta gactaggtat actcataaac 10201 atatgagcaa aaggatagag gagattacat tgcatcttct acaaatttat ttattgccct 10261 ttactgaaaa attaaataat gagtactaaa tgataaaaag cgctcagtac aagaaagatt 10321 gcaaaaatat tgcattcttc atgaattaaa gttgcatata aggcatattg aaagtaatag 10381 tactaaaaca gcagttagcg aaaattaata gaattatatt cgcaagacaa ttgtgacatt 10441 aaattaaaaa attgtagaat ttttactatc ctctttaacg ccatgagcct ttataaaaag 10501 gttaaattag ttttaacatt ctttttttga gtaagagttt acaatttatc aaaacctgtg 10561 ttattatatt cattagtttc aatttattag catctagaga aaaatcaatt ggcagttaca 10621 ttgttggaat ttatgaagaa aaagaatcta caacggagaa taagttgctg atcgctttcc 10681 ctaaaattgt atattttgct gagcttattt tgacatttcg ttgaagtttt ctctaattcg 10741 cattcatttt aagtaaaaca atgagaaata aaattacaaa aaatacaaat taaaatacaa 10801 tttttagcta taattataga cgatgccctt gtatcccatt ctgtctcgct tgcccctact 10861 ttttatcttt tatataccat aatgaacgct gccgctacta accatacccc gattttacat 10921 ttcggactcc caaggacgta caaaatagaa aactatagaa aaaaataatc agaaaatagc 10981 atgtcatctc tttgtaaaac gcgtttgcaa gaagaaagga aacaatggag aagagatcat 11041 ccatttgtat gtaaatttta gtaaacttga agaaatcact aacaacttct cttacttagg 11101 gattctatgc aaaaccttgt aaatcatctg atggaggact cgatttaatg aattggaagg 11161 ttggccatcg attatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc 11221 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat 11281 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt 11341 catcaccgaa acgcgcga //