LOCUS Exported 11370 bp ds-DNA circular SYN 03-JAN-2017 DEFINITION pMZ374 sequence 11384 bps. ACCESSION . VERSION . KEYWORDS SnapGene DNA SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11370) AUTHORS . TITLE Direct Submission JOURNAL Exported Friday, Jun 2, 2017 from SnapGene 3.3.4 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11370 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 75..322 /gene="S. cerevisiae CYC1" /label=CYC1 terminator /note="transcription terminator for CYC1" CDS complement(338..358) /codon_start=1 /product="nuclear localization signal of SV40 large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS complement(371..4474) /codon_start=1 /product="Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=Cas9 /note="generates RNA-guided double strand breaks in DNA" /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" promoter 5265..5369 /gene="bla" /label=AmpR promoter CDS 5370..6230 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 6401..6989 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 7277..7298 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 7313..7343 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7351..7367 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 7375..7391 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" misc_feature 7428..7784 /label=rrk1 gene -1 to -358 misc_feature 7765..7784 /label=20bp misc_feature 7785..7804 /label=gRNA misc_feature 7805..7842 /label=sgRNA Cassette misc_feature 7805..7824 /label=20bp primer_bind complement(7990..8006) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" CDS complement(8647..9441) /codon_start=1 /gene="Schizosaccharomyces pombe ura4" /product="orotidine 5'-phosphate decarboxylase, required for uracil biosynthesis" /label=S. pombe ura4 /translation="MDARVFQSYSARAEGMKNPIAKELLALMEEKQSNLSVAVDLTKKS EILELVDKIGPYVCVIKTHIDVVEDFDQDMVEKLVALGKKHRFLIFEDRKFADIGNTVK LQYASGVYKIASWAHITNCHTVPGEGIIQGLKEVGLPLGRGLLLLAEMSSKGSLATGSY TEKTLEWFEKHTDFCFGFIAGRRFPNLQSDYITMSPGIGLDVKGDGLGQQYRTPEEVIV NCGSDIIIVGRGVYGAGRNPVVEAKRYREAGWKAYQQRLSQH" rep_origin 10051..10832 /label=ars1 /note="Schizosaccharomyces pombe autonomously replicating sequence ars1" ORIGIN 1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 61 cttagacggg gatcgcaaat taaagccttc gagcgtccca aaaccttctc aagcaaggtt 121 ttcagtataa tgttacatgc gtacacgcgt ctgtacagaa aaaaaagaaa aatttgaaat 181 ataaataacg ttcttaatac taacataact ataaaaaaat aaatagggac ctagacttca 241 ggttgtctaa ctccttcctt ttcggttaga gcggatgtgg ggggagggcg tgaatgtaag 301 cgtgacataa ctaattacat gactcgagaa gagatcacac cttcctcttc ttcttggggt 361 cagccctgct gtctccaccg agctgagaga ggtcgattct tgtttcatag agccccgtaa 421 ttgactgatg aatcagtgtg gcgtccagga cctcctttgt agaggtgtac cgctttctgt 481 ctatggtggt gtcgaagtac ttgaaggctg caggcgcgcc caagttggtc agagtaaaca 541 agtggataat gttttctgcc tgctccctga tgggcttatc cctgtgctta ttgtaagcag 601 aaagcacctt atcgaggtta gcgtcggcga ggatcactct tttggagaat tcgcttattt 661 gctcgatgat ctcatcaagg tagtgtttgt gttgttccac gaacagctgc ttctgctcat 721 tatcttcggg agaccctttg agcttttcat agtggctggc cagatacaag aaattaacgt 781 atttagaggg cagtgccagc tcgttacctt tctgcagctc gcccgcacta gcgagcattc 841 gtttccggcc gttttcaagc tcaaagagag agtacttggg aagcttaatg atgaggtctt 901 ttttgacctc tttatatcct ttcgcctcga gaaagtcgat ggggtttttt tcgaagcttg 961 atcgctccat gattgtgatg cccagcagtt ccttgacgct tttgagtttt ttagacttcc 1021 ctttctccac tttggccaca accagtacac tgtaagcgac tgtaggagaa tcgaatccgc 1081 cgtatttctt ggggtcccaa tcttttttgc gtgcgatcag cttgtcgctg ttccttttcg 1141 ggaggatact ttccttggag aagcctccgg tctgtacttc ggtcttttta acgatgttca 1201 cctgcggcat ggacaggacc ttccggactg tcgcgaaatc cctacccttg tcccacacga 1261 tttctcctgt ttctccgttt gtttcgataa gtggtcgctt ccgaatctct ccattggcca 1321 gtgtaatctc ggtcttgaaa aaattcataa tattgctgta aaagaagtac ttagcggtgg 1381 ccttgcctat ttcctgctca gactttgcga tcattttcct aacatcgtac actttatagt 1441 ctccgtaaac aaattcagat tcaagcttgg gatatttttt gataagtgca gtgcctacca 1501 ctgcattcag gtaggcatca tgcgcatggt ggtaattgtt gatctctctc accttataaa 1561 actgaaagtc ctttctgaaa tctgagacca gcttagactt cagagtaata actttcacct 1621 ctcgaatcag tttgtcattt tcatcgtact tggtgttcat gcgtgaatcg agaatttggg 1681 ccacgtgctt ggtgatctgg cgtgtctcaa caagctgcct tttgatgaag ccggctttat 1741 ccaactcaga caggccacct cgttcagcct tagtcagatt atcgaacttc cgttgtgtga 1801 tcagtttggc gttcagcagc tgccgccaat aatttttcat tttcttgaca acttcttctg 1861 aggggacgtt atcactcttc cctctatttt tatcggatct tgtcaacact ttattatcaa 1921 tagaatcatc tttgagaaaa gactggggca cgatatgatc cacgtcgtag tcggagagcc 1981 gattgatgtc cagttcctga tccacgtaca tgtccctgcc gttctgcagg tagtacaggt 2041 agagcttctc attctgaagc tgggtgtttt caactgggtg ttccttaagg atttgggacc 2101 ccagttcttt tataccctct tcaatcctct tcatcctttc cctactgttc ttctgtccct 2161 tctgggtagt ttggttctct cgggccatct cgataacgat attctcgggc ttatgccttc 2221 ccattacttt gacgagttca tccacgacct taacggtctg cagtattccc tttttgatag 2281 ctgggctacc tgcaagatta gcgatgtgct cgtgaagact gtccccctgg ccagaaactt 2341 gtgctttctg gatgtcctcc ttaaaggtga gagagtcatc atggatcaac tgcatgaagt 2401 tccggttggc aaatccatcg gacttaagaa aatccaggat tgtctttcca gactgcttat 2461 ctcggatccc attgatcagt tttcttgaca gccgccccca tcctgtatat cggcgcctct 2521 tgagctgttt catgactttg tcgtcgaaga gatgagcgta agttttcaag cgttcttcaa 2581 tcatctccct atcttcaaac aacgtaaggg tgaggacaat gtcctcaaga atgtcctcgt 2641 tctcctcatt gtccaggaag tccttgtctt taatgatttt caggagatcg tgatacgttc 2701 ccagggatgc gttgaagcga tcctccactc cgctgatttc aacagagtcg aaacattcaa 2761 tctttttgaa atagtcttct ttgagctgtt tcacggtaac tttccggttc gtcttgaaga 2821 ggaggtccac gatagctttc ttctgctctc cagacaggaa tgctggcttt ctcatccctt 2881 ctgtgacgta tttgaccttg gtgagctcgt tataaactgt gaagtactcg tacagcagag 2941 agtgtttagg aagcaccttt tcgttaggca gatttttatc aaagttagtc atcctttcga 3001 tgaaggactg ggcagaggcc cccttatcca cgacttcctc gaagttccag ggagtgatgg 3061 tctcttctga tttgcgagtc atccacgcga atctggaatt tccccgggcg agggggccta 3121 catagtaggg tatccgaaat gtgaggattt tctcaatctt ttccctgtta tctttcaaaa 3181 aggggtagaa atcctcttgc cgcctgagga tagcgtgcag ttcgcccagg tgaatctggt 3241 gggggatgct tccattgtcg aaagtgcgct gtttgcgcaa cagatcttct ctgttaagct 3301 ttaccagcag ctcctcggtg ccgtccattt tttccaagat gggcttaata aatttgtaaa 3361 attcctcctg gcttgctccg ccgtcaatgt atccggcgta gccattttta gactgatcga 3421 agaaaatttc cttgtacttc tcaggcagtt gctgtctgac aagggccttc agcaaagtca 3481 agtcttggtg gtgctcatca tagcgcttga tcatactagc gctcagcgga gctttggtga 3541 tctccgtgtt cactcgcaga atatcactca gcagaatggc gtctgacagg ttctttgccg 3601 ccaaaaaaag gtctgcgtac tggtcgccga tctgggccag cagattgtcg agatcatcat 3661 cgtaggtgtc tttgctcagt tgaagcttgg catcttcggc caggtcgaag ttagatttaa 3721 agttgggggt cagcccgagt gacagggcga taagattacc aaacaggccg ttcttcttct 3781 ccccagggag ctgtgcgatg aggttttcga gccgccggga tttggacagc ctagcgctca 3841 ggattgcttt ggcgtcaact ccggatgcgt tgatcgggtt ctcttcgaaa agctgattgt 3901 aagtctgaac cagttggata aagagtttgt cgacatcgct gttgtctggg ttcaggtccc 3961 cctcgatgag gaagtgtccc cgaaatttga tcatatgcgc cagcgcgaga tagatcaacc 4021 gcaagtcagc cttatcagta ctgtctacaa gcttcttcct cagatgatat atggttgggt 4081 acttttcatg gtacgccacc tcgtccacga tattgccaaa gattgggtgg cgctcgtgct 4141 ttttatcctc ctccaccaaa aaggactcct ccagcctatg gaagaaagag tcatccacct 4201 tagccatctc attactaaag atctcctgca ggtagcagat ccgattcttt ctgcgggtat 4261 atctgcgccg tgctgttctt ttgagccgcg tggcttcggc cgtctccccg gagtcgaaca 4321 ggagggcgcc aatgaggttc ttctttatgc tgtggcgatc ggtattgccc agaactttga 4381 attttttgct cggcaccttg tactcgtccg taatgacggc ccaaccgacg ctgtttgtgc 4441 cgatatcgag cccaatggag tacttcttgt ccatctctag agtcgacctg cagcaattct 4501 cttgcttaaa gaaaagcgaa ggcacctgtc caccacccac tatttatacc atgggaggcg 4561 tcaaaccgag tttttgcgaa aggattatcg cacctctccc caagtaacat cggcaaagca 4621 aaaacggagc ggaagaccaa tgagacgcgg aaggggagcg ccgaatgagg aaagcgagag 4681 agaaagaagt ggatggaatg attccccgcc aaagtggaca catttcggga atcttcagga 4741 agcatggatc accttatgga caagaagata atccggaagc caataggagg gcgacagggg 4801 gtcagcgata agcgtcggaa aaaactttat gatatggtaa atacgtagga ggttacggtg 4861 cagaggaggg gccggtagcg agtgatagcg agggaaagac gatcgctatc gggccgtaag 4921 gaggaaatgg gaggttgggg gaaggatgcg ttggaatgcg gagtagagaa attgaagaga 4981 gagagaggac acacctagag agaaagaaat ggatatagag aaagagaaaa aataagagga 5041 atacggatac gatggagtgc gttgaatgag agaaaagaaa agaaaaggaa tgataagaga 5101 aggaatgaaa attgttttgt tttgttttca attgctttct tttgttttct ttgtctttgt 5161 tcttccttcc cttgtcccga tgctgttctt ctcaccagct tccgcatgat agccattttc 5221 ttagttgttg tagggcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt 5281 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa 5341 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta 5401 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag 5461 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca 5521 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta 5581 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc 5641 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc 5701 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca 5761 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc 5821 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca 5881 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac 5941 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg 6001 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg 6061 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg 6121 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac 6181 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc 6241 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct 6301 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc 6361 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc 6421 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg 6481 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa 6541 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc 6601 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt 6661 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa 6721 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc 6781 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc 6841 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct 6901 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat 6961 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc 7021 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg 7081 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc 7141 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg 7201 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca 7261 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact 7321 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa 7381 acagctatga catgattacg aattcgagct cggtaccccg ggtacctttt gcttatgttg 7441 gtggtagttg gcatgcgtag actgatgact agtcagcaag gagcgtagaa cagtcacact 7501 cgttatatat gtgcttccaa gaaaactcaa gaatttacca ttagcaaaca cttttttgaa 7561 atgttagaca tttaaatgac gaaggcatat agaagctttg aataggtgtt gtaaagtgtt 7621 gatttatgtg acgctgaggg tgcgcatgaa aggaatgttg ggtcacgatt attaaacagt 7681 ttgctagctt ggacacttga gtattggaag ttgttgaatt ctaaaaaact ttcagttgat 7741 ttgaatagtt gctgttgcca aaaaacataa cctgtaccga agaacaacac cgttaagtgt 7801 acgagtttta gagctagaaa tagcaagtta aaataaggct agtccgttat caacttgaaa 7861 aaagtgagtg gcaccgagtc ggtggtgccc tgtcaccgga tgtgctttcc ggtctgatga 7921 gtccgtgagg acgaaacagg ggtaccgtgg ggatcctcta gagtcgacct gcaggcatgc 7981 aagcttggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca 8041 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg 8101 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta 8161 ttttctcctt acgcatctgt gcggtatttc acaccgcata ccatcgcgat gagcttgtga 8221 tattgacgaa actttttgac atctaattta ttctgttcca acaccaatgt ttataaccaa 8281 gttttatctt gtttgtctac atggtatttt acattcatct acatacatct ttcattggct 8341 ttgtacatag ttatcattac aagtctaaaa aaattcactc ttttcttatt caatgtcaat 8401 ccaagagaaa agattgtggt aatgttgtag gagcatgttt aataaattac tatagcaaat 8461 tactttttat tcccaaggtg tttatctata atagttaata ttttagtcgc tacataaaat 8521 tttaccaaag agtacttgta tactaattct aaatgccttc tgacataaaa cgcctaggaa 8581 aacaaacgca aacaaggcat cgactttttc aataaccaac caaaaaaatt ttacattagt 8641 ctttttttaa tgctgagaaa gtctttgctg atatgccttc caaccagctt ctctatatct 8701 cttggcttcg acaacaggat tacgaccagc tccatagact ccacgaccaa caatgatgat 8761 atcgctaccg cagtttacaa tcacttcttc aggagtacga tattgctgtc ccagcccgtc 8821 tcctttaaca tccaagccga taccagggga catagttatg tagtcgcttt gaaggttagg 8881 aaatcgacga ccagctataa agccaaagca aaaatcggta tgcttctcaa accattctaa 8941 ggttttctct gtgtaggaac cagtagccaa agagcctttg gaagacattt cagccaaaag 9001 caagagacca cgtcccaaag gtaaaccaac ttctttgagg ccttgtataa taccctcgcc 9061 tggcactgta tggcaatttg tgatatgagc ccaagaagca attttgtaca caccagatgc 9121 atattgtagc ttgacggtat ttccaatgtc tgcgaatttg cgatcctcaa agataagaaa 9181 acgatgcttt ttacctaagg ccaccagttt ttctaccata tcctggtcga aatcctcgac 9241 aacgtcaata tgtgtcttga taacacagac atagggtcca attttatcta ccaattctaa 9301 gatttcggat ttcttcgtca aatcgaccgc gactgacaag ttgctttgct tttcttccat 9361 caaagccaac aattccttgg caatgggatt tttcatcccc tcagctctag ctgaatagct 9421 ttgaaatact ctagcatcca taactttgct tttaaacctt taatttcgat ccaagcaaaa 9481 aagaggttct tggtaggaca atacggtaag aaaacacgac atgtgcagag atgccgacga 9541 agcatagtta aactgggatg gtaaaatcaa ttaagaattt ataaagacaa aattgtataa 9601 gtctctaaaa catcttaatt atacctcaca gaactatcta aaatatattc acaaagtgca 9661 aacattatca tgaaaaagaa ccattttaat ttaaagcaag ggcattaagg cttatttaca 9721 gaatttctta cttttgtaaa gattataagg ctgattatct ttttcaccat gccaaaaatt 9781 acacaagata gaatggatgt ttgaaattaa acgtgagtat acaaacaaat acactaggta 9841 aatcgaaaca tttttttctc cattaagtaa caaattccta tttagagaaa gaatgctgag 9901 tagattaaat aatctataca aactttttta acacaaatgc atacatatag ccagtgggat 9961 ttgtagctaa gctcatcgat ggcgagtcta actccttaac cactcggaca tagtgactta 10021 tctgacactc attgtaaatt aatatatata taggcatttt gtttagttaa aggtacttaa 10081 gtaattagta taaacgaacc aattttataa tcaggaagtt aagtgaatgg tagcacatgt 10141 cgtaaaaatt gtgaattttt attgaataat attttaaata caagcctttc tagactaggt 10201 atactcataa acatatgagc aaaaggatag aggagattac attgcatctt ctacaaattt 10261 atttattgcc ctttactgaa aaattaaata atgagtacta aatgataaaa agcgctcagt 10321 acaagaaaga ttgcaaaaat attgcattct tcatgaatta aagttgcata taaggcatat 10381 tgaaagtaat agtactaaaa cagcagttag cgaaaattaa tagaattata ttcgcaagac 10441 aattgtgaca ttaaattaaa aaattgtaga atttttacta tcctctttaa cgccatgagc 10501 ctttataaaa aggttaaatt agttttaaca ttcttttttt gagtaagagt ttacaattta 10561 tcaaaacctg tgttattata ttcattagtt tcaatttatt agcatctaga gaaaaatcaa 10621 ttggcagtta cattgttgga atttatgaag aaaaagaatc tacaacggag aataagttgc 10681 tgatcgcttt ccctaaaatt gtatattttg ctgagcttat tttgacattt cgttgaagtt 10741 ttctctaatt cgcattcatt ttaagtaaaa caatgagaaa taaaattaca aaaaatacaa 10801 attaaaatac aatttttagc tataattata gacgatgccc ttgtatccca ttctgtctcg 10861 cttgccccta ctttttatct tttatatacc ataatgaacg ctgccgctac taaccatacc 10921 ccgattttac atttcggact cccaaggacg tacaaaatag aaaactatag aaaaaaataa 10981 tcagaaaata gcatgtcatc tctttgtaaa acgcgtttgc aagaagaaag gaaacaatgg 11041 agaagagatc atccatttgt atgtaaattt tagtaaactt gaagaaatca ctaacaactt 11101 ctcttactta gggattctat gcaaaacctt gtaaatcatc tgatggagga ctcgatttaa 11161 tgaattggaa ggttggccat cgattatggt gcactctcag tacaatctgc tctgatgccg 11221 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc 11281 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga 11341 ggttttcacc gtcatcaccg aaacgcgcga //