LOCUS       Exported               11370 bp ds-DNA     circular SYN 05-JAN-2017
DEFINITION  pMZ374 sequence 11384 bps.
ACCESSION   .
VERSION     .
KEYWORDS    SnapGene DNA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11370)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jun 2, 2017 from SnapGene 3.3.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11370
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      75..322
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     CDS             complement(338..358)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(371..4474)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     promoter        5265..5369
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5370..6230
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6401..6989
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    7277..7298
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7313..7343
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7351..7367
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7375..7391
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    7428..7784
                     /label=rrk1 gene -1 to -358
     misc_feature    7765..7784
                     /label=20bp
     misc_feature    7785..7804
                     /label=gRNA
     misc_feature    7805..7842
                     /label=sgRNA Cassette
     misc_feature    7805..7824
                     /label=20bp
     primer_bind     complement(7990..8006)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(8647..9441)
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe ura4"
                     /product="orotidine 5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=S. pombe ura4
                     /translation="MDARVFQSYSARAEGMKNPIAKELLALMEEKQSNLSVAVDLTKKS
                     EILELVDKIGPYVCVIKTHIDVVEDFDQDMVEKLVALGKKHRFLIFEDRKFADIGNTVK
                     LQYASGVYKIASWAHITNCHTVPGEGIIQGLKEVGLPLGRGLLLLAEMSSKGSLATGSY
                     TEKTLEWFEKHTDFCFGFIAGRRFPNLQSDYITMSPGIGLDVKGDGLGQQYRTPEEVIV
                     NCGSDIIIVGRGVYGAGRNPVVEAKRYREAGWKAYQQRLSQH"
     rep_origin      10051..10832
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacggg gatcgcaaat taaagccttc gagcgtccca aaaccttctc aagcaaggtt
      121 ttcagtataa tgttacatgc gtacacgcgt ctgtacagaa aaaaaagaaa aatttgaaat
      181 ataaataacg ttcttaatac taacataact ataaaaaaat aaatagggac ctagacttca
      241 ggttgtctaa ctccttcctt ttcggttaga gcggatgtgg ggggagggcg tgaatgtaag
      301 cgtgacataa ctaattacat gactcgagaa gagatcacac cttcctcttc ttcttggggt
      361 cagccctgct gtctccaccg agctgagaga ggtcgattct tgtttcatag agccccgtaa
      421 ttgactgatg aatcagtgtg gcgtccagga cctcctttgt agaggtgtac cgctttctgt
      481 ctatggtggt gtcgaagtac ttgaaggctg caggcgcgcc caagttggtc agagtaaaca
      541 agtggataat gttttctgcc tgctccctga tgggcttatc cctgtgctta ttgtaagcag
      601 aaagcacctt atcgaggtta gcgtcggcga ggatcactct tttggagaat tcgcttattt
      661 gctcgatgat ctcatcaagg tagtgtttgt gttgttccac gaacagctgc ttctgctcat
      721 tatcttcggg agaccctttg agcttttcat agtggctggc cagatacaag aaattaacgt
      781 atttagaggg cagtgccagc tcgttacctt tctgcagctc gcccgcacta gcgagcattc
      841 gtttccggcc gttttcaagc tcaaagagag agtacttggg aagcttaatg atgaggtctt
      901 ttttgacctc tttatatcct ttcgcctcga gaaagtcgat ggggtttttt tcgaagcttg
      961 atcgctccat gattgtgatg cccagcagtt ccttgacgct tttgagtttt ttagacttcc
     1021 ctttctccac tttggccaca accagtacac tgtaagcgac tgtaggagaa tcgaatccgc
     1081 cgtatttctt ggggtcccaa tcttttttgc gtgcgatcag cttgtcgctg ttccttttcg
     1141 ggaggatact ttccttggag aagcctccgg tctgtacttc ggtcttttta acgatgttca
     1201 cctgcggcat ggacaggacc ttccggactg tcgcgaaatc cctacccttg tcccacacga
     1261 tttctcctgt ttctccgttt gtttcgataa gtggtcgctt ccgaatctct ccattggcca
     1321 gtgtaatctc ggtcttgaaa aaattcataa tattgctgta aaagaagtac ttagcggtgg
     1381 ccttgcctat ttcctgctca gactttgcga tcattttcct aacatcgtac actttatagt
     1441 ctccgtaaac aaattcagat tcaagcttgg gatatttttt gataagtgca gtgcctacca
     1501 ctgcattcag gtaggcatca tgcgcatggt ggtaattgtt gatctctctc accttataaa
     1561 actgaaagtc ctttctgaaa tctgagacca gcttagactt cagagtaata actttcacct
     1621 ctcgaatcag tttgtcattt tcatcgtact tggtgttcat gcgtgaatcg agaatttggg
     1681 ccacgtgctt ggtgatctgg cgtgtctcaa caagctgcct tttgatgaag ccggctttat
     1741 ccaactcaga caggccacct cgttcagcct tagtcagatt atcgaacttc cgttgtgtga
     1801 tcagtttggc gttcagcagc tgccgccaat aatttttcat tttcttgaca acttcttctg
     1861 aggggacgtt atcactcttc cctctatttt tatcggatct tgtcaacact ttattatcaa
     1921 tagaatcatc tttgagaaaa gactggggca cgatatgatc cacgtcgtag tcggagagcc
     1981 gattgatgtc cagttcctga tccacgtaca tgtccctgcc gttctgcagg tagtacaggt
     2041 agagcttctc attctgaagc tgggtgtttt caactgggtg ttccttaagg atttgggacc
     2101 ccagttcttt tataccctct tcaatcctct tcatcctttc cctactgttc ttctgtccct
     2161 tctgggtagt ttggttctct cgggccatct cgataacgat attctcgggc ttatgccttc
     2221 ccattacttt gacgagttca tccacgacct taacggtctg cagtattccc tttttgatag
     2281 ctgggctacc tgcaagatta gcgatgtgct cgtgaagact gtccccctgg ccagaaactt
     2341 gtgctttctg gatgtcctcc ttaaaggtga gagagtcatc atggatcaac tgcatgaagt
     2401 tccggttggc aaatccatcg gacttaagaa aatccaggat tgtctttcca gactgcttat
     2461 ctcggatccc attgatcagt tttcttgaca gccgccccca tcctgtatat cggcgcctct
     2521 tgagctgttt catgactttg tcgtcgaaga gatgagcgta agttttcaag cgttcttcaa
     2581 tcatctccct atcttcaaac aacgtaaggg tgaggacaat gtcctcaaga atgtcctcgt
     2641 tctcctcatt gtccaggaag tccttgtctt taatgatttt caggagatcg tgatacgttc
     2701 ccagggatgc gttgaagcga tcctccactc cgctgatttc aacagagtcg aaacattcaa
     2761 tctttttgaa atagtcttct ttgagctgtt tcacggtaac tttccggttc gtcttgaaga
     2821 ggaggtccac gatagctttc ttctgctctc cagacaggaa tgctggcttt ctcatccctt
     2881 ctgtgacgta tttgaccttg gtgagctcgt tataaactgt gaagtactcg tacagcagag
     2941 agtgtttagg aagcaccttt tcgttaggca gatttttatc aaagttagtc atcctttcga
     3001 tgaaggactg ggcagaggcc cccttatcca cgacttcctc gaagttccag ggagtgatgg
     3061 tctcttctga tttgcgagtc atccacgcga atctggaatt tccccgggcg agggggccta
     3121 catagtaggg tatccgaaat gtgaggattt tctcaatctt ttccctgtta tctttcaaaa
     3181 aggggtagaa atcctcttgc cgcctgagga tagcgtgcag ttcgcccagg tgaatctggt
     3241 gggggatgct tccattgtcg aaagtgcgct gtttgcgcaa cagatcttct ctgttaagct
     3301 ttaccagcag ctcctcggtg ccgtccattt tttccaagat gggcttaata aatttgtaaa
     3361 attcctcctg gcttgctccg ccgtcaatgt atccggcgta gccattttta gactgatcga
     3421 agaaaatttc cttgtacttc tcaggcagtt gctgtctgac aagggccttc agcaaagtca
     3481 agtcttggtg gtgctcatca tagcgcttga tcatactagc gctcagcgga gctttggtga
     3541 tctccgtgtt cactcgcaga atatcactca gcagaatggc gtctgacagg ttctttgccg
     3601 ccaaaaaaag gtctgcgtac tggtcgccga tctgggccag cagattgtcg agatcatcat
     3661 cgtaggtgtc tttgctcagt tgaagcttgg catcttcggc caggtcgaag ttagatttaa
     3721 agttgggggt cagcccgagt gacagggcga taagattacc aaacaggccg ttcttcttct
     3781 ccccagggag ctgtgcgatg aggttttcga gccgccggga tttggacagc ctagcgctca
     3841 ggattgcttt ggcgtcaact ccggatgcgt tgatcgggtt ctcttcgaaa agctgattgt
     3901 aagtctgaac cagttggata aagagtttgt cgacatcgct gttgtctggg ttcaggtccc
     3961 cctcgatgag gaagtgtccc cgaaatttga tcatatgcgc cagcgcgaga tagatcaacc
     4021 gcaagtcagc cttatcagta ctgtctacaa gcttcttcct cagatgatat atggttgggt
     4081 acttttcatg gtacgccacc tcgtccacga tattgccaaa gattgggtgg cgctcgtgct
     4141 ttttatcctc ctccaccaaa aaggactcct ccagcctatg gaagaaagag tcatccacct
     4201 tagccatctc attactaaag atctcctgca ggtagcagat ccgattcttt ctgcgggtat
     4261 atctgcgccg tgctgttctt ttgagccgcg tggcttcggc cgtctccccg gagtcgaaca
     4321 ggagggcgcc aatgaggttc ttctttatgc tgtggcgatc ggtattgccc agaactttga
     4381 attttttgct cggcaccttg tactcgtccg taatgacggc ccaaccgacg ctgtttgtgc
     4441 cgatatcgag cccaatggag tacttcttgt ccatctctag agtcgacctg cagcaattct
     4501 cttgcttaaa gaaaagcgaa ggcacctgtc caccacccac tatttatacc atgggaggcg
     4561 tcaaaccgag tttttgcgaa aggattatcg cacctctccc caagtaacat cggcaaagca
     4621 aaaacggagc ggaagaccaa tgagacgcgg aaggggagcg ccgaatgagg aaagcgagag
     4681 agaaagaagt ggatggaatg attccccgcc aaagtggaca catttcggga atcttcagga
     4741 agcatggatc accttatgga caagaagata atccggaagc caataggagg gcgacagggg
     4801 gtcagcgata agcgtcggaa aaaactttat gatatggtaa atacgtagga ggttacggtg
     4861 cagaggaggg gccggtagcg agtgatagcg agggaaagac gatcgctatc gggccgtaag
     4921 gaggaaatgg gaggttgggg gaaggatgcg ttggaatgcg gagtagagaa attgaagaga
     4981 gagagaggac acacctagag agaaagaaat ggatatagag aaagagaaaa aataagagga
     5041 atacggatac gatggagtgc gttgaatgag agaaaagaaa agaaaaggaa tgataagaga
     5101 aggaatgaaa attgttttgt tttgttttca attgctttct tttgttttct ttgtctttgt
     5161 tcttccttcc cttgtcccga tgctgttctt ctcaccagct tccgcatgat agccattttc
     5221 ttagttgttg tagggcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     5281 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     5341 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     5401 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     5461 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     5521 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     5581 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     5641 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     5701 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     5761 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     5821 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     5881 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     5941 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     6001 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     6061 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     6121 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     6181 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     6241 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     6301 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     6361 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     6421 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     6481 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     6541 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     6601 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     6661 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     6721 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     6781 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     6841 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     6901 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     6961 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     7021 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     7081 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     7141 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     7201 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     7261 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     7321 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     7381 acagctatga catgattacg aattcgagct cggtaccccg ggtacctttt gcttatgttg
     7441 gtggtagttg gcatgcgtag actgatgact agtcagcaag gagcgtagaa cagtcacact
     7501 cgttatatat gtgcttccaa gaaaactcaa gaatttacca ttagcaaaca cttttttgaa
     7561 atgttagaca tttaaatgac gaaggcatat agaagctttg aataggtgtt gtaaagtgtt
     7621 gatttatgtg acgctgaggg tgcgcatgaa aggaatgttg ggtcacgatt attaaacagt
     7681 ttgctagctt ggacacttga gtattggaag ttgttgaatt ctaaaaaact ttcagttgat
     7741 ttgaatagtt gctgttgcca aaaaacataa cctgtaccga agaaatttat tggatataat
     7801 gacagtttta gagctagaaa tagcaagtta aaataaggct agtccgttat caacttgaaa
     7861 aaagtgagtg gcaccgagtc ggtggtgccc tgtcaccgga tgtgctttcc ggtctgatga
     7921 gtccgtgagg acgaaacagg ggtaccgtgg ggatcctcta gagtcgacct gcaggcatgc
     7981 aagcttggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     8041 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     8101 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta
     8161 ttttctcctt acgcatctgt gcggtatttc acaccgcata ccatcgcgat gagcttgtga
     8221 tattgacgaa actttttgac atctaattta ttctgttcca acaccaatgt ttataaccaa
     8281 gttttatctt gtttgtctac atggtatttt acattcatct acatacatct ttcattggct
     8341 ttgtacatag ttatcattac aagtctaaaa aaattcactc ttttcttatt caatgtcaat
     8401 ccaagagaaa agattgtggt aatgttgtag gagcatgttt aataaattac tatagcaaat
     8461 tactttttat tcccaaggtg tttatctata atagttaata ttttagtcgc tacataaaat
     8521 tttaccaaag agtacttgta tactaattct aaatgccttc tgacataaaa cgcctaggaa
     8581 aacaaacgca aacaaggcat cgactttttc aataaccaac caaaaaaatt ttacattagt
     8641 ctttttttaa tgctgagaaa gtctttgctg atatgccttc caaccagctt ctctatatct
     8701 cttggcttcg acaacaggat tacgaccagc tccatagact ccacgaccaa caatgatgat
     8761 atcgctaccg cagtttacaa tcacttcttc aggagtacga tattgctgtc ccagcccgtc
     8821 tcctttaaca tccaagccga taccagggga catagttatg tagtcgcttt gaaggttagg
     8881 aaatcgacga ccagctataa agccaaagca aaaatcggta tgcttctcaa accattctaa
     8941 ggttttctct gtgtaggaac cagtagccaa agagcctttg gaagacattt cagccaaaag
     9001 caagagacca cgtcccaaag gtaaaccaac ttctttgagg ccttgtataa taccctcgcc
     9061 tggcactgta tggcaatttg tgatatgagc ccaagaagca attttgtaca caccagatgc
     9121 atattgtagc ttgacggtat ttccaatgtc tgcgaatttg cgatcctcaa agataagaaa
     9181 acgatgcttt ttacctaagg ccaccagttt ttctaccata tcctggtcga aatcctcgac
     9241 aacgtcaata tgtgtcttga taacacagac atagggtcca attttatcta ccaattctaa
     9301 gatttcggat ttcttcgtca aatcgaccgc gactgacaag ttgctttgct tttcttccat
     9361 caaagccaac aattccttgg caatgggatt tttcatcccc tcagctctag ctgaatagct
     9421 ttgaaatact ctagcatcca taactttgct tttaaacctt taatttcgat ccaagcaaaa
     9481 aagaggttct tggtaggaca atacggtaag aaaacacgac atgtgcagag atgccgacga
     9541 agcatagtta aactgggatg gtaaaatcaa ttaagaattt ataaagacaa aattgtataa
     9601 gtctctaaaa catcttaatt atacctcaca gaactatcta aaatatattc acaaagtgca
     9661 aacattatca tgaaaaagaa ccattttaat ttaaagcaag ggcattaagg cttatttaca
     9721 gaatttctta cttttgtaaa gattataagg ctgattatct ttttcaccat gccaaaaatt
     9781 acacaagata gaatggatgt ttgaaattaa acgtgagtat acaaacaaat acactaggta
     9841 aatcgaaaca tttttttctc cattaagtaa caaattccta tttagagaaa gaatgctgag
     9901 tagattaaat aatctataca aactttttta acacaaatgc atacatatag ccagtgggat
     9961 ttgtagctaa gctcatcgat ggcgagtcta actccttaac cactcggaca tagtgactta
    10021 tctgacactc attgtaaatt aatatatata taggcatttt gtttagttaa aggtacttaa
    10081 gtaattagta taaacgaacc aattttataa tcaggaagtt aagtgaatgg tagcacatgt
    10141 cgtaaaaatt gtgaattttt attgaataat attttaaata caagcctttc tagactaggt
    10201 atactcataa acatatgagc aaaaggatag aggagattac attgcatctt ctacaaattt
    10261 atttattgcc ctttactgaa aaattaaata atgagtacta aatgataaaa agcgctcagt
    10321 acaagaaaga ttgcaaaaat attgcattct tcatgaatta aagttgcata taaggcatat
    10381 tgaaagtaat agtactaaaa cagcagttag cgaaaattaa tagaattata ttcgcaagac
    10441 aattgtgaca ttaaattaaa aaattgtaga atttttacta tcctctttaa cgccatgagc
    10501 ctttataaaa aggttaaatt agttttaaca ttcttttttt gagtaagagt ttacaattta
    10561 tcaaaacctg tgttattata ttcattagtt tcaatttatt agcatctaga gaaaaatcaa
    10621 ttggcagtta cattgttgga atttatgaag aaaaagaatc tacaacggag aataagttgc
    10681 tgatcgcttt ccctaaaatt gtatattttg ctgagcttat tttgacattt cgttgaagtt
    10741 ttctctaatt cgcattcatt ttaagtaaaa caatgagaaa taaaattaca aaaaatacaa
    10801 attaaaatac aatttttagc tataattata gacgatgccc ttgtatccca ttctgtctcg
    10861 cttgccccta ctttttatct tttatatacc ataatgaacg ctgccgctac taaccatacc
    10921 ccgattttac atttcggact cccaaggacg tacaaaatag aaaactatag aaaaaaataa
    10981 tcagaaaata gcatgtcatc tctttgtaaa acgcgtttgc aagaagaaag gaaacaatgg
    11041 agaagagatc atccatttgt atgtaaattt tagtaaactt gaagaaatca ctaacaactt
    11101 ctcttactta gggattctat gcaaaacctt gtaaatcatc tgatggagga ctcgatttaa
    11161 tgaattggaa ggttggccat cgattatggt gcactctcag tacaatctgc tctgatgccg
    11221 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
    11281 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
    11341 ggttttcacc gtcatcaccg aaacgcgcga
//
