LOCUS       Exported                4898 bp ds-DNA     circular SYN 06-FEB-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    SnapGene DNA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jun 2, 2017 from SnapGene 3.3.4
            http://www.snapgene.com
COMMENT     RFP Gibsoned into SmaI site of pTZ19u
FEATURES             Location/Qualifiers
     source          1..4898
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     240..259
                     /label=T7
     misc_feature    318..1317
                     /label=upstream homologous region
     misc_feature    752..1197
                     /label=ina17
     CDS             join(752..853,962..1197)
                     /codon_start=1
                     /translation="MLRKLPINFAKWTVKKVPVQQKRFNSQQKEISPHIMFYKNYARPL
                     GKVTLFALATYYGLEIVWWKLDASEQEAIKNSKLLICESSFSLLTFRRITEFRECEIKT
                     RDLYDPEI"
     misc_feature    1318..2317
                     /label=downstream homolgous region
     misc_feature    complement(1490..2317)
                     /label=php5
     CDS             complement(1936..2317)
                     /codon_start=1
                     /translation="NVNQMNMQRPVMVAPYMAEHLYRYPPTHLESGSSAFRLQSSPMGY
                     QMPQFQGNMRPNMQQSQMFDPSAYGMSRRPGSPRQFDQQQRLYSQPNAMMYQTQQGRQG
                     NPMHQQFSQQQNPLSRYSQQPQ"
     primer_bind     complement(2353..2369)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2441..2509
                     /label=LacZ alpha
     rep_origin      2528..2834
                     /label=F1 ori
     promoter        2993..3097
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3098..3958
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             join(3296..3813,3815..3955)
                     /label=AmpR
     rep_origin      4053..4735
                     /label=ColE1 origin
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct
      241 aatacgactc actataggga aagcttgcat gcctgcaggt cgactctaga ggatcggccg
      301 gcccgcggcc gcacagtgcg agcacttgga ttttaacctc ctaatttgta aacggtactg
      361 tatctttctt tttataattt ttgagtcgaa gcatgttctg ctgcataaca cattcatatt
      421 tgtcaattct acttaaatag attttaaatc attgcattcg aagataaaat tatggtagct
      481 tagtgttcct acggatctta atgtatttat atgttcactt tataatacga tacaaaacaa
      541 attttatctc tcctttgttt caaattgact ggcaaaaaaa aataacaaat aactaatcac
      601 aaaaacctaa ggaatatgaa aaatagttgc taattttcgt agaaacttgg atcttgatta
      661 gaaatgaacc aaattatgca tcattcaaat agattgtagt gtgtatggtg cagcgggaac
      721 gttataatta accatcacct atagtctcaa aatgttacgc aaattgccca tcaattttgc
      781 gaaatggact gtaaaaaagg tgccagttca gcagaaaaga tttaatagtc aacaaaaaga
      841 aatctcacct catgtaagct tttctaaata cgctttaggt cataaatttt atctgtgcta
      901 aaattcattc aagcattttt gcattgtttt atttaacatt attttattaa cgatcataaa
      961 gattatgttt tataaaaact acgctagacc tcttggtaaa gtgacgcttt ttgccttagc
     1021 aacttattat ggattggaaa tagtttggtg gaaattagat gcatcagaac aggaagctat
     1081 taaaaacagt aagttgttga tatgtgaaag ctcttttagt ttgttgacat ttaggcgaat
     1141 tacggaattt agagaatgcg aaataaaaac acgagatttg tatgacccag aaatttagta
     1201 acgtatatgg cttaaagaag cgagattacg atttgttaat agttcagtat gtcaaaaatt
     1261 ctttgaatag ctaaatgcta cagacgaaac actcagtcaa aaggtcctcg agttgttgaa
     1321 tggtggtttt ttatgcagtc gggaatgaaa gcagctgtaa tttccttgca taatgagcct
     1381 aaataccttt ttacatagaa gttaaaattt atgagatttt aaattaaaaa atcatcaaaa
     1441 gtgcaaattt tcaagtacat atacgaataa atttaattat aaagtaaacc taaacgttgt
     1501 ttaaagctaa ttgcttcttg gtaaccgatt gcaacatgcc atcatttatt tacattgaca
     1561 acccgtaaat atccataata ggtagaatgt tatcgacaat gaatttcaaa tgcatatata
     1621 tgtttaagta aaacaaaata ctctacacta gcagtcccgc aatgggaaaa taaagaggca
     1681 gcaaatatga aaagcttccg tcatttcttt ttcggtcttg ctaaaaaaat aaaaccacac
     1741 atagcttaaa aaaagtaaca atgactagac atagcatgtc atgaaaatca actgtttctt
     1801 taagcttttg tagataaacc gaaaattgac aaattgaaag ttccacctaa aaatgcatca
     1861 ctgttggatc ctcccaaata atttaataga aattatttaa attgcatgtt ctaaatcaac
     1921 aggtattaaa agatattact ggggttgttg cgaatagcga gaaagagggt tttgttgctg
     1981 ggaaaactgc tgatgcattg ggtttccctg acgcccttgc tgagtttgat acatcatagc
     2041 gtttggctgt gaataaagtc tctgttgctg atcgaattgc cgaggagatc caggtcttct
     2101 tgacatacca tatgcagaag gatcaaacat ttgagactgt tgcatattag gtcgcatatt
     2161 accttgaaat tggggcattt gatatcccat aggggatgac tgcaatcgga aagctgagga
     2221 tccagattcg agatgcgtag gtggataacg gtataaatgc tcagccatgt acggtgctac
     2281 catgaccggt ctttgcatat tcatctggtt aacattttag cggccgcggg ccggcctacc
     2341 gagctcgaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     2401 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     2461 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg
     2521 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     2581 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     2641 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     2701 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     2761 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     2821 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     2881 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     2941 taacaaaata ttaacgttta caatttcagg tggcactttt cggggaaatg tgcgcggaac
     3001 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     3061 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     3121 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     3181 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     3241 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     3301 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     3361 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     3421 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     3481 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     3541 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     3601 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     3661 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     3721 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     3781 tattgctgat aaatctggag ccggtgagcg tggatctcgc ggtatcattg cagcactggg
     3841 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     3901 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     3961 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     4021 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     4081 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     4141 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     4201 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     4261 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     4321 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     4381 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     4441 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     4501 gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga gaaaggcgga
     4561 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     4621 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     4681 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     4741 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     4801 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     4861 gaccgagcgc agcgagtcag tgagcgagga agcggaag
//
