LOCUS       018_BB1_L_12_syn_BsaI_2      1966 bp    DNA     circular UNA 
DEFINITION  Order Date: 22/12/2014
            RedID:68195111
            IDT DNA
FEATURES             Location/Qualifiers
     CDS             join(1265..1966,1..117)
                     /label=KanR
     oriT            163..778
                     /label="Origin of Replication"
     Region          complement(163..178)
                     /label=Rest_F1_ori
     Region          complement(163..778)
                     /label="Homology to pSTBlue"
     Primer          805..822
                     /label=Seq_BB1_FW
     Region          937..994
                     /label=Linker
     FS              948..951
                     /label=FS1
     FS              980..983
                     /label=FS2
     Primer          complement(1066..1082)
                     /label=Seq_BB1_BW
ORIGIN
        1 TGCCTCGGTG AGTTTTCTCC TTCATTACAG AAACGGCTTT TTCAAAAATA TGGTATTGAT
       61 AATCCTGATA TGAATAAATT GCAGTTTCAC TTGATGCTCG ATGAGTTTTT CTAATGAGGA
      121 CCTAAATGTA ATCACCTGGC TCACCTTCGG GTGGGCCTTT CTGCGTTGCT GGCGTTTTTC
      181 CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAT GCTCAAGTCA GAGGTGGCGA
      241 AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT
      301 CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG
      361 GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG
      421 CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT
      481 CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC
      541 AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC
      601 TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTCG
      661 GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT
      721 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATTT
      781 TCTACCGAAG AAAGGCCCAC CCGTGTAAAA CGACGGCCAG TTTATCTAGT CAGCTTGATT
      841 CTAGCTGATC GTGGACCGGA AGGTGAGCCA GTGAGTTGAT TGCAGTCCAG TTACGCTGGA
      901 GTCTGAGGCT CGTCCTGAAT GATATGCGGC CTCGAATTCG AAGACGCGGA GTGAGACCGG
      961 ATCCATCTCG AGGGTCTCAC ATGGCGTCTT CGAATTCGCG CGTGATCTTA CGGCATTATA
     1021 CGTATGATCG GTCCACGATC AGCTAGATTA TCTAGTCAGC TTGATGTCAT AGCTGTTTCC
     1081 TGAGGCTCAA TACTGACCAT TTAAATCATA CCTGACCTCC ATAGCAGAAA GTCAAAAGCC
     1141 TCCGACCGGA GGCTTTTGAC TTGATCGGCA CGTAAGAGGT TCCAACTTTC ACCATAATGA
     1201 AATAAGATCA CTACCGGGCG TATTTTTTGA GTTATCGAGA TTTTCAGGAG CTAAGGAAGC
     1261 TAAAATGAGC CATATTCAAC GGGAAACGTC TTGCTTGAAG CCGCGATTAA ATTCCAACAT
     1321 GGATGCTGAT TTATATGGGT ATAAATGGGC TCGCGATAAT GTCGGGCAAT CAGGTGCGAC
     1381 AATCTATCGA TTGTATGGGA AGCCCGATGC GCCAGAGTTG TTTCTGAAAC ATGGCAAAGG
     1441 TAGCGTTGCC AATGATGTTA CAGATGAGAT GGTCAGGCTA AACTGGCTGA CGGAATTTAT
     1501 GCCTCTTCCG ACCATCAAGC ATTTTATCCG TACTCCTGAT GATGCATGGT TACTCACCAC
     1561 TGCGATCCCA GGGAAAACAG CATTCCAGGT ATTAGAAGAA TATCCTGATT CAGGTGAAAA
     1621 TATTGTTGAT GCGCTGGCAG TGTTCCTGCG CCGGTTGCAT TCGATTCCTG TTTGTAATTG
     1681 TCCTTTTAAC GGCGATCGCG TATTTCGTCT CGCTCAGGCG CAATCACGAA TGAATAACGG
     1741 TTTGGTTGGT GCGAGTGATT TTGATGACGA GCGTAATGGC TGGCCTGTTG AACAAGTCTG
     1801 GAAAGAAATG CATAAACTCT TGCCATTCTC ACCGGATTCA GTCGTCACTC ATGGTGATTT
     1861 CTCACTTGAT AACCTTATTT TTGACGAGGG GAAATTAATA GGTTGTATTG ATGTTGGACG
     1921 AGTCGGAATC GCAGACCGAT ACCAGGATCT TGCCATCCTA TGGAAC
//
