LOCUS       070_BB1_23_L_syn_BsaI_1_v2      1966 bp    DNA     circular UNA 07-Jan-2015
DEFINITION  old name: BB1_linker_FS2_BsaI_FS3
FEATURES             Location/Qualifiers
     CDS             complement(join(1850..1966,1..702))
                     /label=KanR
     Primer          885..901
                     /label="Seq_BB1_BW (B3_63)"
     FS              complement(984..987)
                     /label=FS3
     FS              complement(1016..1019)
                     /label=FS2
     Primer          complement(1145..1162)
                     /label="Seq_BB1_FW (B3_62)"
     oriT            complement(1189..1804)
                     /label="Origin of Replication"
ORIGIN
        1 GTTCCATAGG ATGGCAAGAT CCTGGTATCG GTCTGCGATT CCGACTCGTC CAACATCAAT
       61 ACAACCTATT AATTTCCCCT CGTCAAAAAT AAGGTTATCA AGTGAGAAAT CACCATGAGT
      121 GACGACTGAA TCCGGTGAGA ATGGCAAGAG TTTATGCATT TCTTTCCAGA CTTGTTCAAC
      181 AGGCCAGCCA TTACGCTCGT CATCAAAATC ACTCGCACCA ACCAAACCGT TATTCATTCG
      241 TGATTGCGCC TGAGCGAGAC GAAATACGCG ATCGCCGTTA AAAGGACAAT TACAAACAGG
      301 AATCGAATGC AACCGGCGCA GGAACACTGC CAGCGCATCA ACAATATTTT CACCTGAATC
      361 AGGATATTCT TCTAATACCT GGAATGCTGT TTTCCCTGGG ATCGCAGTGG TGAGTAACCA
      421 TGCATCATCA GGAGTACGGA TAAAATGCTT GATGGTCGGA AGAGGCATAA ATTCCGTCAG
      481 CCAGTTTAGC CTGACCATCT CATCTGTAAC ATCATTGGCA ACGCTACCTT TGCCATGTTT
      541 CAGAAACAAC TCTGGCGCAT CGGGCTTCCC ATACAATCGA TAGATTGTCG CACCTGATTG
      601 CCCGACATTA TCGCGAGCCC ATTTATACCC ATATAAATCA GCATCCATGT TGGAATTTAA
      661 TCGCGGCTTC AAGCAAGACG TTTCCCGTTG AATATGGCTC ATTTTAGCTT CCTTAGCTCC
      721 TGAAAATCTC GATAACTCAA AAAATACGCC CGGTAGTGAT CTTATTTCAT TATGGTGAAA
      781 GTTGGAACCT CTTACGTGCC GATCAAGTCA AAAGCCTCCG GTCGGAGGCT TTTGACTTTC
      841 TGCTATGGAG GTCAGGTATG ATTTAAATGG TCAGTATTGA GCCTCAGGAA ACAGCTATGA
      901 CATCAAGCTG ACTAGATAAT CTAGCTGATC GTGGACCGAT CATACGTATA ATGCCGTAAG
      961 ATCACGCGCG AATTCGAAGA CGCCATGTGA GACCCTCGAG ATGGATCCGG TCTCAGCTTG
     1021 CGTCTTCGAA TTCGAGGCCG CATATCATTC AGGACGAGCC TCAGACTCCA GCGTAACTGG
     1081 ACTGCAATCA ACTCACTGGC TCACCTTCCG GTCCACGATC AGCTAGAATC AAGCTGACTA
     1141 GATAAACTGG CCGTCGTTTT ACACGGGTGG GCCTTTCTTC GGTAGAAAAT CAAAGGATCT
     1201 TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA
     1261 CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAG GTAACTGGCT
     1321 TCAGCAGAGC GCAGATACCA AATACTGTTC TTCTAGTGTA GCCGTAGTTA GGCCACCACT
     1381 TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG
     1441 CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA
     1501 AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA
     1561 CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG
     1621 GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG
     1681 AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC
     1741 TTGAGCATCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA
     1801 ACGCAGAAAG GCCCACCCGA AGGTGAGCCA GGTGATTACA TTTAGGTCCT CATTAGAAAA
     1861 ACTCATCGAG CATCAAGTGA AACTGCAATT TATTCATATC AGGATTATCA ATACCATATT
     1921 TTTGAAAAAG CCGTTTCTGT AATGAAGGAG AAAACTCACC GAGGCA
//
