LOCUS       028_BB1_12_pPOR1      2637 bp    DNA     circular UNA 
DEFINITION  Order Date: 22/12/2014
            RedID:68195111
            IDT DNA
FEATURES             Location/Qualifiers
     CDS             join(1936..2637,1..117)
                     /label=KanR
     oriT            163..778
                     /label=ORI
     Primer          805..822
                     /label=Seq_BB1_FW
     Fusion_Site     948..951
                     /label=FS1
     Promoter        952..>1650
                     /label=pPOR1
     Restriction_sit join(1650^1651,complement(1650^1651))
                     /note="Name: BbsI"
                     /note="Pattern: gaagac"
                     /note="Number_of_matches: 2"
                     /note="cut_0_on_positive_strand: 2860^2861"
                     /note="cut_0_on_negative_strand: 2864^2865"
                     /note="inhibited_by: "
                     /note="site_type: other"
                     /note="restriction site"
                     /label=BbsI
     Fusion_Site     1651..1654
                     /label=FS2
     Primer          complement(1737..1753)
                     /label=Seq_BB1_BW
ORIGIN
        1 TGCCTCGGTG AGTTTTCTCC TTCATTACAG AAACGGCTTT TTCAAAAATA TGGTATTGAT
       61 AATCCTGATA TGAATAAATT GCAGTTTCAC TTGATGCTCG ATGAGTTTTT CTAATGAGGA
      121 CCTAAATGTA ATCACCTGGC TCACCTTCGG GTGGGCCTTT CTGCGTTGCT GGCGTTTTTC
      181 CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAT GCTCAAGTCA GAGGTGGCGA
      241 AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT
      301 CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG
      361 GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG
      421 CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT
      481 CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC
      541 AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC
      601 TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTCG
      661 GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT
      721 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATTT
      781 TCTACCGAAG AAAGGCCCAC CCGTGTAAAA CGACGGCCAG TTTATCTAGT CAGCTTGATT
      841 CTAGCTGATC GTGGACCGGA AGGTGAGCCA GTGAGTTGAT TGCAGTCCAG TTACGCTGGA
      901 GTCTGAGGCT CGTCCTGAAT GATATGCGGC CTCGAATTCG AAGACGCGGA GCGCATTATT
      961 TGGGTCGGCT ACTGTCATTG GTTATGATGG CCTTACCGAG CACGAAGGTG GTTGGATAGA
     1021 CCACAACTGG AAACAGTTGT ACAAACAGAT TGCATTCATT TTTGCTTGTA TTGGATACTC
     1081 GATGGCCATC ACCGCTCTTA TCTGTTTCAT CCTCAACCGT ATTCCATTTT TGCAACTGCG
     1141 AGCTTCAGAA GAGGCTGAGG AGAAAGGTAT GGATGAGGAT CAGATTGGAG AGTTCGCTTA
     1201 TGACTACGTG GAAGTACGTC GTGATTTTTT GGCTTGGGGA TCAGGCCCAA ACAATGGCTT
     1261 CAAGGAGCCG GAAGTTCTGG ATCAGGTAGT TCCGGTTAAT GATTTCAGCA GTGACCAGAA
     1321 TGTGACTAAT CAGACCAACG AATCTGAGAA GCAGTAGAGT AAATATAGAG ATGATATTTA
     1381 GTGTATTCTA ATGCTTATGT AATGTATTAA GCAAAAAGTT GTGTTTATGA GTTAGCATTT
     1441 GTCTTAGCAA ACATAAAATT ATGTCGACAT TTGCAACCCG CATGTCTAGT GTTTTTAGAT
     1501 CGATCTTCGA TGTGTAGAAT AATGCCTCCA CGTGATGCCC CGCGATTTTG TTGGGTCGCA
     1561 ATGCCTCCAA CATAAACCCA TCACGTATAA AAAGCCCTCT TAACCCTCCC CCCTGTTTCG
     1621 TTTGCTTCAT CACTTAACCT GAACTATCAA CATGGCGTCT TCGAATTCGC GCGTGATCTT
     1681 ACGGCATTAT ACGTATGATC GGTCCACGAT CAGCTAGATT ATCTAGTCAG CTTGATGTCA
     1741 TAGCTGTTTC CTGAGGCTCA ATACTGACCA TTTAAATCAT ACCTGACCTC CATAGCAGAA
     1801 AGTCAAAAGC CTCCGACCGG AGGCTTTTGA CTTGATCGGC ACGTAAGAGG TTCCAACTTT
     1861 CACCATAATG AAATAAGATC ACTACCGGGC GTATTTTTTG AGTTATCGAG ATTTTCAGGA
     1921 GCTAAGGAAG CTAAAATGAG CCATATTCAA CGGGAAACGT CTTGCTTGAA GCCGCGATTA
     1981 AATTCCAACA TGGATGCTGA TTTATATGGG TATAAATGGG CTCGCGATAA TGTCGGGCAA
     2041 TCAGGTGCGA CAATCTATCG ATTGTATGGG AAGCCCGATG CGCCAGAGTT GTTTCTGAAA
     2101 CATGGCAAAG GTAGCGTTGC CAATGATGTT ACAGATGAGA TGGTCAGGCT AAACTGGCTG
     2161 ACGGAATTTA TGCCTCTTCC GACCATCAAG CATTTTATCC GTACTCCTGA TGATGCATGG
     2221 TTACTCACCA CTGCGATCCC AGGGAAAACA GCATTCCAGG TATTAGAAGA ATATCCTGAT
     2281 TCAGGTGAAA ATATTGTTGA TGCGCTGGCA GTGTTCCTGC GCCGGTTGCA TTCGATTCCT
     2341 GTTTGTAATT GTCCTTTTAA CGGCGATCGC GTATTTCGTC TCGCTCAGGC GCAATCACGA
     2401 ATGAATAACG GTTTGGTTGG TGCGAGTGAT TTTGATGACG AGCGTAATGG CTGGCCTGTT
     2461 GAACAAGTCT GGAAAGAAAT GCATAAACTC TTGCCATTCT CACCGGATTC AGTCGTCACT
     2521 CATGGTGATT TCTCACTTGA TAACCTTATT TTTGACGAGG GGAAATTAAT AGGTTGTATT
     2581 GATGTTGGAC GAGTCGGAAT CGCAGACCGA TACCAGGATC TTGCCATCCT ATGGAAC
//
