LOCUS       026_BB1_12_pMDH3      2912 bp    DNA     circular UNA 
DEFINITION  Order Date: 22/12/2014
            RedID:68195111
            IDT DNA
FEATURES             Location/Qualifiers
     CDS             join(2211..2912,1..117)
                     /label=KanR
     oriT            163..778
                     /label=ORI
     Primer          805..822
                     /label=Seq_BB1_FW
     Fusion_Site     948..951
                     /label=FS1
     Promoter        951..>1925
                     /origin="Fragment (FR839631_Chr4 (pMDH3_FW - pMDH3_RV))
                     (1.004bp linear)Fragment: from BsaI cut at 11^12 to BsaI
                     cut at 989^990, 978bpOrientation: originalPlus strand 5':
                     no changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
                     /label=pMDH3
     Fusion_Site     1926..1929
                     /label=FS2
     Primer          complement(2012..2028)
                     /label=Seq_BB1_BW
ORIGIN
        1 TGCCTCGGTG AGTTTTCTCC TTCATTACAG AAACGGCTTT TTCAAAAATA TGGTATTGAT
       61 AATCCTGATA TGAATAAATT GCAGTTTCAC TTGATGCTCG ATGAGTTTTT CTAATGAGGA
      121 CCTAAATGTA ATCACCTGGC TCACCTTCGG GTGGGCCTTT CTGCGTTGCT GGCGTTTTTC
      181 CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAT GCTCAAGTCA GAGGTGGCGA
      241 AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT
      301 CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG
      361 GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG
      421 CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT
      481 CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC
      541 AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC
      601 TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTCG
      661 GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT
      721 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATTT
      781 TCTACCGAAG AAAGGCCCAC CCGTGTAAAA CGACGGCCAG TTTATCTAGT CAGCTTGATT
      841 CTAGCTGATC GTGGACCGGA AGGTGAGCCA GTGAGTTGAT TGCAGTCCAG TTACGCTGGA
      901 GTCTGAGGCT CGTCCTGAAT GATATGCGGC CTCGAATTCG AAGACGCGGA GTAGCTTGGG
      961 TAGGACTTGA CAAGTACGGC TTCCGTGGTC ATACCAAACG CCTTTGTTAC CGTTGGCTAT
     1021 ACCTAATGAC CAAGGCATTT GTGGATTATA ACGGTATCGT AGTTGAAAAA TATGACGTAA
     1081 CCACTGGTAC TAGCCCCCAC AAGGTTGATG CTGAATACGG GAATCAAGGT GCCGATTTTA
     1141 AAGGAGTAGC CACTGAAGGG TTTGGCTGGG TCAATGCCTC TTTTATTTTG GGATTAACCT
     1201 ACTTAGATGT CCAAGGCATC CGTGCGATAG GCGCCGTTAC GTCCCCTGAT GTATTTTTCA
     1261 GGAAGCTCAA ACCTTGGGAA CGCGCAAGTT ATGGCCTAAG GCCATGTAAC GAGATAGTCA
     1321 AGTCAAACTA GAAGTATACG GTTTCCCCGC AGAAATAGCA GAAATAGGCG ACAAATACAT
     1381 ACAACATTTT CATTGTGATA GGGGGCGGCG GTTCCTAGGA GGGACAACCC CCAGAAACCT
     1441 TGTAGACTAC GTTTTCACGA CGATGGGTTA TTACTGTAAA GGAAGAATAT ACTACCCACC
     1501 AGTTGAATGT TTGAACGGAT CAAAGGTCGA AGGGAGTACA CGGCCCAACC AACGTAGCTA
     1561 CCGGAGAAAG CAAGACTTTC CCAAACCAAA TAGCTCCGGG TTTCTTCTCC GGCAACCCGT
     1621 CAGTTTTTGT GTGGCCGGAC AAAAATTCGC ACCCTCAGTC TAATTGAAAG GTCGGGCTCC
     1681 GAGCTCTAGG CGTTTGCGCA TGTAATATTG CATCCCCTCC CATAGATAAT ACTGCGCGAA
     1741 CACAGGGTGC AAATTATGAT GACCACACAT GCCAGTGACC AAAACAGTTT TTTAGTCTTT
     1801 AAAAACCCTC GGAACTTCTG AGTATATAAA GGCTTCTCAT TTCCTACAAG CAAACAAAGA
     1861 AGAAACTTCC ACTTTCTAAC TTTTTATCTA TAGACTTTAG AGTTACAACC AACGAACAAT
     1921 AACAACATGG CGTCTTCGAA TTCGCGCGTG ATCTTACGGC ATTATACGTA TGATCGGTCC
     1981 ACGATCAGCT AGATTATCTA GTCAGCTTGA TGTCATAGCT GTTTCCTGAG GCTCAATACT
     2041 GACCATTTAA ATCATACCTG ACCTCCATAG CAGAAAGTCA AAAGCCTCCG ACCGGAGGCT
     2101 TTTGACTTGA TCGGCACGTA AGAGGTTCCA ACTTTCACCA TAATGAAATA AGATCACTAC
     2161 CGGGCGTATT TTTTGAGTTA TCGAGATTTT CAGGAGCTAA GGAAGCTAAA ATGAGCCATA
     2221 TTCAACGGGA AACGTCTTGC TTGAAGCCGC GATTAAATTC CAACATGGAT GCTGATTTAT
     2281 ATGGGTATAA ATGGGCTCGC GATAATGTCG GGCAATCAGG TGCGACAATC TATCGATTGT
     2341 ATGGGAAGCC CGATGCGCCA GAGTTGTTTC TGAAACATGG CAAAGGTAGC GTTGCCAATG
     2401 ATGTTACAGA TGAGATGGTC AGGCTAAACT GGCTGACGGA ATTTATGCCT CTTCCGACCA
     2461 TCAAGCATTT TATCCGTACT CCTGATGATG CATGGTTACT CACCACTGCG ATCCCAGGGA
     2521 AAACAGCATT CCAGGTATTA GAAGAATATC CTGATTCAGG TGAAAATATT GTTGATGCGC
     2581 TGGCAGTGTT CCTGCGCCGG TTGCATTCGA TTCCTGTTTG TAATTGTCCT TTTAACGGCG
     2641 ATCGCGTATT TCGTCTCGCT CAGGCGCAAT CACGAATGAA TAACGGTTTG GTTGGTGCGA
     2701 GTGATTTTGA TGACGAGCGT AATGGCTGGC CTGTTGAACA AGTCTGGAAA GAAATGCATA
     2761 AACTCTTGCC ATTCTCACCG GATTCAGTCG TCACTCATGG TGATTTCTCA CTTGATAACC
     2821 TTATTTTTGA CGAGGGGAAA TTAATAGGTT GTATTGATGT TGGACGAGTC GGAATCGCAG
     2881 ACCGATACCA GGATCTTGCC ATCCTATGGA AC
//
