LOCUS       019_BB1_12_pADH2      2753 bp    DNA     circular UNA 
DEFINITION  Order Date: 22/12/2014
            RedID:68195111
            IDT DNA
FEATURES             Location/Qualifiers
     CDS             join(2052..2753,1..117)
                     /label=KanR
     oriT            163..778
                     /label=ORI
     Primer          805..822
                     /label=Seq_BB1_FW
     Fusion_Site     948..951
                     /label=FS1
     Promoter        952..>1766
                     /origin="Fragment (FR839629_Chr2 (pADH2_RV - pADH2_FW))
                     (845bp linear)Fragment: from BsaI cut at 11^12 to BsaI
                     cut at 830^831, 819bpOrientation: reverse
                     complementedPlus strand 5':   no changePlus strand 3':
                     no changeMinus strand 5':  no changeMinus strand 3':  no
                     change"
                     /label=pADH2
     Fusion_Site     1767..1770
                     /label=FS2
     Primer          complement(1853..1869)
                     /label=Seq_BB1_BW
ORIGIN
        1 TGCCTCGGTG AGTTTTCTCC TTCATTACAG AAACGGCTTT TTCAAAAATA TGGTATTGAT
       61 AATCCTGATA TGAATAAATT GCAGTTTCAC TTGATGCTCG ATGAGTTTTT CTAATGAGGA
      121 CCTAAATGTA ATCACCTGGC TCACCTTCGG GTGGGCCTTT CTGCGTTGCT GGCGTTTTTC
      181 CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAT GCTCAAGTCA GAGGTGGCGA
      241 AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT
      301 CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG
      361 GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG
      421 CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT
      481 CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC
      541 AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC
      601 TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTCG
      661 GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT
      721 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATTT
      781 TCTACCGAAG AAAGGCCCAC CCGTGTAAAA CGACGGCCAG TTTATCTAGT CAGCTTGATT
      841 CTAGCTGATC GTGGACCGGA AGGTGAGCCA GTGAGTTGAT TGCAGTCCAG TTACGCTGGA
      901 GTCTGAGGCT CGTCCTGAAT GATATGCGGC CTCGAATTCG AAGACGCGGA GTGATTATGT
      961 AAGAAGAGGG GGGTGATTCG GCCGGCTATC GAACTCTAAC AACTAGGGGG GTGAACAATG
     1021 CCCAGCAGTC CTCCCCACTC TTTGACAAAT CAGTATCACC GATTAACACC CCAAATCTTA
     1081 TTCTCAACGG TCCCTCATCC TTGCACCCCT CTTTGGACAA ATGGCAGTTA GCATTGGTGC
     1141 ACTGACTGAC TGCCCAACCT TAAACCCAAA TTTCTTAGAA GGGGCCCATC TAGTTAGCGA
     1201 GGGGTGAAAA ATTCCTCCAT CGGAGATGTA TTGACCGTAA GTTGCTGCTT AAAAAAAATC
     1261 AGTTCAGATA GCGAGACTTT TTTGATTTCG CAACGGGAGT GCCTGTTCCA TTCGATTGCA
     1321 ATTCTCACCC CTTCTGCCCA GTCCTGCCAA TTGCCCATGA ATCTGCTAAT TTCGTTGATT
     1381 CCCACCCCCC TTTCCAACTC CACAAATTGT CCAATCTCGT TTTCCATTTG GGAGAATCTG
     1441 CATGTCGACT ACATAAAGCG ACCGGTGTCC GAAAAGATCT GTGTAGTTTT CAACATTTTG
     1501 TGCTCCCCCC GCTGTTTGAA AACGGGGGTG AGCGCTCTCC GGGGTGCGAA TTCGTGCCCA
     1561 ATTCCTTTCA CCCTGCCTAT TGTAGACGTC AACCCGCATC TGGTGCGAAT ATAGCGCACC
     1621 CCCAATGATC ACACCAACAA TTGGTCCACC CCTCCCCAAT CTCTAATATT CACAATTCAC
     1681 CTCACTATAA ATACCCCTGT CCTGCTCCCA AATTCTTTTT TCCTTCTTCC ATCAGCTACT
     1741 AGCTTTTATC TTATTTACTT TACGAACATG GCGTCTTCGA ATTCGCGCGT GATCTTACGG
     1801 CATTATACGT ATGATCGGTC CACGATCAGC TAGATTATCT AGTCAGCTTG ATGTCATAGC
     1861 TGTTTCCTGA GGCTCAATAC TGACCATTTA AATCATACCT GACCTCCATA GCAGAAAGTC
     1921 AAAAGCCTCC GACCGGAGGC TTTTGACTTG ATCGGCACGT AAGAGGTTCC AACTTTCACC
     1981 ATAATGAAAT AAGATCACTA CCGGGCGTAT TTTTTGAGTT ATCGAGATTT TCAGGAGCTA
     2041 AGGAAGCTAA AATGAGCCAT ATTCAACGGG AAACGTCTTG CTTGAAGCCG CGATTAAATT
     2101 CCAACATGGA TGCTGATTTA TATGGGTATA AATGGGCTCG CGATAATGTC GGGCAATCAG
     2161 GTGCGACAAT CTATCGATTG TATGGGAAGC CCGATGCGCC AGAGTTGTTT CTGAAACATG
     2221 GCAAAGGTAG CGTTGCCAAT GATGTTACAG ATGAGATGGT CAGGCTAAAC TGGCTGACGG
     2281 AATTTATGCC TCTTCCGACC ATCAAGCATT TTATCCGTAC TCCTGATGAT GCATGGTTAC
     2341 TCACCACTGC GATCCCAGGG AAAACAGCAT TCCAGGTATT AGAAGAATAT CCTGATTCAG
     2401 GTGAAAATAT TGTTGATGCG CTGGCAGTGT TCCTGCGCCG GTTGCATTCG ATTCCTGTTT
     2461 GTAATTGTCC TTTTAACGGC GATCGCGTAT TTCGTCTCGC TCAGGCGCAA TCACGAATGA
     2521 ATAACGGTTT GGTTGGTGCG AGTGATTTTG ATGACGAGCG TAATGGCTGG CCTGTTGAAC
     2581 AAGTCTGGAA AGAAATGCAT AAACTCTTGC CATTCTCACC GGATTCAGTC GTCACTCATG
     2641 GTGATTTCTC ACTTGATAAC CTTATTTTTG ACGAGGGGAA ATTAATAGGT TGTATTGATG
     2701 TTGGACGAGT CGGAATCGCA GACCGATACC AGGATCTTGC CATCCTATGG AAC
//
