LOCUS       029_BB1_12_pPpPfk      2162 bp    DNA     circular UNA 
DEFINITION  Order Date: 22/12/2014
            RedID:68195111
            IDT DNA
FEATURES             Location/Qualifiers
     CDS             join(1461..2162,1..117)
                     /label=KanR
     oriT            163..778
                     /label=ORI
     Primer          805..822
                     /label=Seq_BB1_FW
     Fusion_Site     948..951
                     /label=FS1
     Promoter        960..1175
                     /label=pPpPfk
     Fusion_Site     1176..1179
                     /label=FS2
     Primer          complement(1262..1278)
                     /label=Seq_BB1_BW
ORIGIN
        1 TGCCTCGGTG AGTTTTCTCC TTCATTACAG AAACGGCTTT TTCAAAAATA TGGTATTGAT
       61 AATCCTGATA TGAATAAATT GCAGTTTCAC TTGATGCTCG ATGAGTTTTT CTAATGAGGA
      121 CCTAAATGTA ATCACCTGGC TCACCTTCGG GTGGGCCTTT CTGCGTTGCT GGCGTTTTTC
      181 CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAT GCTCAAGTCA GAGGTGGCGA
      241 AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT
      301 CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG
      361 GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG
      421 CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT
      481 CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC
      541 AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC
      601 TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTCG
      661 GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT
      721 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATTT
      781 TCTACCGAAG AAAGGCCCAC CCGTGTAAAA CGACGGCCAG TTTATCTAGT CAGCTTGATT
      841 CTAGCTGATC GTGGACCGGA AGGTGAGCCA GTGAGTTGAT TGCAGTCCAG TTACGCTGGA
      901 GTCTGAGGCT CGTCCTGAAT GATATGCGGC CTCGAATTCG AAGACGCGGA GCCTGCCATT
      961 AGGCAAACAC CAACTAGGAA CCTACACCAA GTTATGAAGG AGCTTGGAAA GGATTATAAA
     1021 AAATGCGATT ACCTAATACA CACACGATGC CTGAGCGGTT AATGTGAAAT TTCCCGGCCA
     1081 TCGATCGCCC CACAATCAGA CTATTTTCGC TCATACAATA AAAAGTCTCC TGTTTCCACC
     1141 CGAGTATCAC CAGAACAAAG TAACAAATAC AAAACCATGG CGTCTTCGAA TTCGCGCGTG
     1201 ATCTTACGGC ATTATACGTA TGATCGGTCC ACGATCAGCT AGATTATCTA GTCAGCTTGA
     1261 TGTCATAGCT GTTTCCTGAG GCTCAATACT GACCATTTAA ATCATACCTG ACCTCCATAG
     1321 CAGAAAGTCA AAAGCCTCCG ACCGGAGGCT TTTGACTTGA TCGGCACGTA AGAGGTTCCA
     1381 ACTTTCACCA TAATGAAATA AGATCACTAC CGGGCGTATT TTTTGAGTTA TCGAGATTTT
     1441 CAGGAGCTAA GGAAGCTAAA ATGAGCCATA TTCAACGGGA AACGTCTTGC TTGAAGCCGC
     1501 GATTAAATTC CAACATGGAT GCTGATTTAT ATGGGTATAA ATGGGCTCGC GATAATGTCG
     1561 GGCAATCAGG TGCGACAATC TATCGATTGT ATGGGAAGCC CGATGCGCCA GAGTTGTTTC
     1621 TGAAACATGG CAAAGGTAGC GTTGCCAATG ATGTTACAGA TGAGATGGTC AGGCTAAACT
     1681 GGCTGACGGA ATTTATGCCT CTTCCGACCA TCAAGCATTT TATCCGTACT CCTGATGATG
     1741 CATGGTTACT CACCACTGCG ATCCCAGGGA AAACAGCATT CCAGGTATTA GAAGAATATC
     1801 CTGATTCAGG TGAAAATATT GTTGATGCGC TGGCAGTGTT CCTGCGCCGG TTGCATTCGA
     1861 TTCCTGTTTG TAATTGTCCT TTTAACGGCG ATCGCGTATT TCGTCTCGCT CAGGCGCAAT
     1921 CACGAATGAA TAACGGTTTG GTTGGTGCGA GTGATTTTGA TGACGAGCGT AATGGCTGGC
     1981 CTGTTGAACA AGTCTGGAAA GAAATGCATA AACTCTTGCC ATTCTCACCG GATTCAGTCG
     2041 TCACTCATGG TGATTTCTCA CTTGATAACC TTATTTTTGA CGAGGGGAAA TTAATAGGTT
     2101 GTATTGATGT TGGACGAGTC GGAATCGCAG ACCGATACCA GGATCTTGCC ATCCTATGGA
     2161 AC
//
