LOCUS       048_BB1_12_pDAS2      2838 bp    DNA     circular UNA 
DEFINITION  Order Date: 22/12/2014
            RedID:68195111
            IDT DNA
FEATURES             Location/Qualifiers
     CDS             join(2137..2838,1..117)
                     /label=KanR
     oriT            163..778
                     /label=ORI
     Primer          805..822
                     /label=Seq_BB1_FW
     Fusion_Site     948..951
                     /label=FS1
     Promoter        952..1851
                     /label=pDAS2
     FS              1852..1855
                     /label=FS2
     Fusion_Site     1852..1855
                     /label=FS2
     Primer          complement(1938..1954)
                     /label=Seq_BB1_BW
ORIGIN
        1 TGCCTCGGTG AGTTTTCTCC TTCATTACAG AAACGGCTTT TTCAAAAATA TGGTATTGAT
       61 AATCCTGATA TGAATAAATT GCAGTTTCAC TTGATGCTCG ATGAGTTTTT CTAATGAGGA
      121 CCTAAATGTA ATCACCTGGC TCACCTTCGG GTGGGCCTTT CTGCGTTGCT GGCGTTTTTC
      181 CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAT GCTCAAGTCA GAGGTGGCGA
      241 AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT
      301 CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG
      361 GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG
      421 CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT
      481 CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC
      541 AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC
      601 TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTCG
      661 GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT
      721 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATTT
      781 TCTACCGAAG AAAGGCCCAC CCGTGTAAAA CGACGGCCAG TTTATCTAGT CAGCTTGATT
      841 CTAGCTGATC GTGGACCGGA AGGTGAGCCA GTGAGTTGAT TGCAGTCCAG TTACGCTGGA
      901 GTCTGAGGCT CGTCCTGAAT GATATGCGGC CTCGAATTCG AAGACGCGGA GCAATCTGTG
      961 GTTTGCTAAA CTGGAAGTCT GGTAAGGACT CTAGCAAGTC CGTTACTCAA AAAGTCATAC
     1021 CAAGTAAGAT TACGTAACAC CTGGGCATGA CTTTCTAAGT TAGCAAGTCA CCAAGAGGGT
     1081 CCTATTTAAC GTTTGGCGGT ATCTGAAACA CAAGACTTGC CTATCCCATA GTACATCATA
     1141 TTACCTGTCA AGCTATGCTA CCCCACAGAA ATACCCCAAA AGTTGAAGTG AAAAAATGAA
     1201 AATTACTGGT AACTTCACCC CATAACAAAC TTAATAATTT CTGTAGCCAA TGAAAGTAAA
     1261 CCCCATTCAA TGTTCCGAGA TTTAGTATAC TTGCCCCTAT AAGAAACGAA GGATTTCAGC
     1321 TTCCTTACCC CATGAACAGA AATCTTCCAT TTACCCCCCA CTGGAGAGAT CCGCCCAAAC
     1381 GAACAGATAA TAGAAAAAAG AAATTCGGAC AAATAGAACA CTTTCTCAGC CAATTAAAGT
     1441 CATTCCATGC ACTCCCTTTA GCTGCCGTTC CATCCCTTTG TTGAGCAACA CCATCGTTAG
     1501 CCAGTACGAA AGAGGAAACT TAACCGATAC CTTGGAGAAA TCTAAGGCGC GAATGAGTTT
     1561 AGCCTAGATA TCCTTAGTGA AGGGTTGTTC CGATACTTCT CCACATTCAG TCATAGATGG
     1621 GCAGCTTTGT TATCATGAAG AGACGGAAAC GGGCATTAAG GGTTAACCGC CAAATTATAT
     1681 AAAGACAACA TGTCCCCAGT TTAAAGTTTT TCTTTCCTAT TCTTGTATCC TGAGTGACCG
     1741 TTGTGTTTAA TATAACAAGT TCGTTTTAAC TTAAGACCAA AACCAGTTAC AACAAATTAT
     1801 AACCCCTCTA AACACTAAAG TTCACTCTTA TCAAACTATC AAACATCAAA ACATGGCGTC
     1861 TTCGAATTCG CGCGTGATCT TACGGCATTA TACGTATGAT CGGTCCACGA TCAGCTAGAT
     1921 TATCTAGTCA GCTTGATGTC ATAGCTGTTT CCTGAGGCTC AATACTGACC ATTTAAATCA
     1981 TACCTGACCT CCATAGCAGA AAGTCAAAAG CCTCCGACCG GAGGCTTTTG ACTTGATCGG
     2041 CACGTAAGAG GTTCCAACTT TCACCATAAT GAAATAAGAT CACTACCGGG CGTATTTTTT
     2101 GAGTTATCGA GATTTTCAGG AGCTAAGGAA GCTAAAATGA GCCATATTCA ACGGGAAACG
     2161 TCTTGCTTGA AGCCGCGATT AAATTCCAAC ATGGATGCTG ATTTATATGG GTATAAATGG
     2221 GCTCGCGATA ATGTCGGGCA ATCAGGTGCG ACAATCTATC GATTGTATGG GAAGCCCGAT
     2281 GCGCCAGAGT TGTTTCTGAA ACATGGCAAA GGTAGCGTTG CCAATGATGT TACAGATGAG
     2341 ATGGTCAGGC TAAACTGGCT GACGGAATTT ATGCCTCTTC CGACCATCAA GCATTTTATC
     2401 CGTACTCCTG ATGATGCATG GTTACTCACC ACTGCGATCC CAGGGAAAAC AGCATTCCAG
     2461 GTATTAGAAG AATATCCTGA TTCAGGTGAA AATATTGTTG ATGCGCTGGC AGTGTTCCTG
     2521 CGCCGGTTGC ATTCGATTCC TGTTTGTAAT TGTCCTTTTA ACGGCGATCG CGTATTTCGT
     2581 CTCGCTCAGG CGCAATCACG AATGAATAAC GGTTTGGTTG GTGCGAGTGA TTTTGATGAC
     2641 GAGCGTAATG GCTGGCCTGT TGAACAAGTC TGGAAAGAAA TGCATAAACT CTTGCCATTC
     2701 TCACCGGATT CAGTCGTCAC TCATGGTGAT TTCTCACTTG ATAACCTTAT TTTTGACGAG
     2761 GGGAAATTAA TAGGTTGTAT TGATGTTGGA CGAGTCGGAA TCGCAGACCG ATACCAGGAT
     2821 CTTGCCATCC TATGGAAC
//
