LOCUS       049_BB1_12_pDAS1      2487 bp    DNA     circular UNA 
DEFINITION  Order Date: 22/12/2014
            RedID:68195111
            IDT DNA
FEATURES             Location/Qualifiers
     CDS             join(1786..2487,1..117)
                     /label=KanR
     oriT            163..778
                     /label=ORI
     Primer          805..822
                     /label=Seq_BB1_FW
     Fusion_Site     948..951
                     /label=FS1
     Promoter        952..1500
                     /label=pDAS1
     Fusion_Site     1501..1504
                     /label=FS2
     Primer          complement(1587..1603)
                     /label=Seq_BB1_BW
ORIGIN
        1 TGCCTCGGTG AGTTTTCTCC TTCATTACAG AAACGGCTTT TTCAAAAATA TGGTATTGAT
       61 AATCCTGATA TGAATAAATT GCAGTTTCAC TTGATGCTCG ATGAGTTTTT CTAATGAGGA
      121 CCTAAATGTA ATCACCTGGC TCACCTTCGG GTGGGCCTTT CTGCGTTGCT GGCGTTTTTC
      181 CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAT GCTCAAGTCA GAGGTGGCGA
      241 AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT
      301 CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG
      361 GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG
      421 CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT
      481 CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC
      541 AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC
      601 TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTCG
      661 GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT
      721 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATTT
      781 TCTACCGAAG AAAGGCCCAC CCGTGTAAAA CGACGGCCAG TTTATCTAGT CAGCTTGATT
      841 CTAGCTGATC GTGGACCGGA AGGTGAGCCA GTGAGTTGAT TGCAGTCCAG TTACGCTGGA
      901 GTCTGAGGCT CGTCCTGAAT GATATGCGGC CTCGAATTCG AAGACGCGGA GAATGATATA
      961 AACAACAATT GAGTGACAGG TCTACTTTGT TCTCAAAAGG CCATAACCAT CTGTTTGCAT
     1021 CTCTTATCAC CACACCATCC TCCTCATCTG GCCTTCAATT GTGGGGAACA ACTAGCATCC
     1081 CAACACCAGA CTAACTCCAC CCAGATGAAA CCAGTTGTCG CTTACCAGTC AATGAATGTT
     1141 GAGCTAACGT TCCTTGAAAC TCGAATGATC CCAGCCTTGC TGCGTATCAT CCCTCCGCTA
     1201 TTCCGCCGCT TGCTCCAACC ATGTTTCCGC CTTTTTCGAA CAAGTTCAAA TACCTATCTT
     1261 TGGCAGGACT TTTCCTCCTG CCTTTTTTAG CCTCAGTTCT CGGTTAGCCT CTAGGCAAAT
     1321 TCTGTTCTTC ATACCTATAT CAACTTTTCA TCAGATAGCC TTTGGGTTCA AAAAAGAACT
     1381 AAAGCAGGAT GCCTGATATA TAAATCCCAG ATGATCTGCT TTTGAAACTA TTTTCAGTAT
     1441 CTTGATTCGT TTACTTACAA ACAACTATTG TTGATTTTAT CTGGAGAATA ATCGAACAAA
     1501 CATGGCGTCT TCGAATTCGC GCGTGATCTT ACGGCATTAT ACGTATGATC GGTCCACGAT
     1561 CAGCTAGATT ATCTAGTCAG CTTGATGTCA TAGCTGTTTC CTGAGGCTCA ATACTGACCA
     1621 TTTAAATCAT ACCTGACCTC CATAGCAGAA AGTCAAAAGC CTCCGACCGG AGGCTTTTGA
     1681 CTTGATCGGC ACGTAAGAGG TTCCAACTTT CACCATAATG AAATAAGATC ACTACCGGGC
     1741 GTATTTTTTG AGTTATCGAG ATTTTCAGGA GCTAAGGAAG CTAAAATGAG CCATATTCAA
     1801 CGGGAAACGT CTTGCTTGAA GCCGCGATTA AATTCCAACA TGGATGCTGA TTTATATGGG
     1861 TATAAATGGG CTCGCGATAA TGTCGGGCAA TCAGGTGCGA CAATCTATCG ATTGTATGGG
     1921 AAGCCCGATG CGCCAGAGTT GTTTCTGAAA CATGGCAAAG GTAGCGTTGC CAATGATGTT
     1981 ACAGATGAGA TGGTCAGGCT AAACTGGCTG ACGGAATTTA TGCCTCTTCC GACCATCAAG
     2041 CATTTTATCC GTACTCCTGA TGATGCATGG TTACTCACCA CTGCGATCCC AGGGAAAACA
     2101 GCATTCCAGG TATTAGAAGA ATATCCTGAT TCAGGTGAAA ATATTGTTGA TGCGCTGGCA
     2161 GTGTTCCTGC GCCGGTTGCA TTCGATTCCT GTTTGTAATT GTCCTTTTAA CGGCGATCGC
     2221 GTATTTCGTC TCGCTCAGGC GCAATCACGA ATGAATAACG GTTTGGTTGG TGCGAGTGAT
     2281 TTTGATGACG AGCGTAATGG CTGGCCTGTT GAACAAGTCT GGAAAGAAAT GCATAAACTC
     2341 TTGCCATTCT CACCGGATTC AGTCGTCACT CATGGTGATT TCTCACTTGA TAACCTTATT
     2401 TTTGACGAGG GGAAATTAAT AGGTTGTATT GATGTTGGAC GAGTCGGAAT CGCAGACCGA
     2461 TACCAGGATC TTGCCATCCT ATGGAAC
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