LOCUS       020_BB1_34_ScCYC1tt      2215 bp    DNA     circular UNA 07-Jan-2015
DEFINITION  
FEATURES             Location/Qualifiers
     CDS             join(1514..2215,1..117)
                     /label=KanR
     oriT            163..778
                     /label=ORI
     Primer          805..822
                     /label=Seq_BB1_FW
     Fusion_Site     948..951
                     /label=FS3
     Terminator      958..1228
                     /label=ScCYC1tt
     Fusion_Site     1229..1232
                     /label=FS4
     Primer          complement(1315..1331)
                     /label=Seq_BB1_BW
ORIGIN
        1 TGCCTCGGTG AGTTTTCTCC TTCATTACAG AAACGGCTTT TTCAAAAATA TGGTATTGAT
       61 AATCCTGATA TGAATAAATT GCAGTTTCAC TTGATGCTCG ATGAGTTTTT CTAATGAGGA
      121 CCTAAATGTA ATCACCTGGC TCACCTTCGG GTGGGCCTTT CTGCGTTGCT GGCGTTTTTC
      181 CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAT GCTCAAGTCA GAGGTGGCGA
      241 AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT
      301 CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG
      361 GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG
      421 CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT
      481 CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC
      541 AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC
      601 TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTCG
      661 GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT
      721 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATTT
      781 TCTACCGAAG AAAGGCCCAC CCGTGTAAAA CGACGGCCAG TTTATCTAGT CAGCTTGATT
      841 CTAGCTGATC GTGGACCGGA AGGTGAGCCA GTGAGTTGAT TGCAGTCCAG TTACGCTGGA
      901 GTCTGAGGCT CGTCCTGAAT GATATGCGGC CTCGAATTCG AAGACGCGCT TCAGGCCCCT
      961 TTTCCTTTGT CGATATCATG TAATTAGTTA TGTCACGCTT ACATTCACGC CCTCCCCCCA
     1021 CATCCGCTCT AACCGAAAAG GAAGGAGTTA GACAACCTGA AGTCTAGGTC CCTATTTATT
     1081 TTTTTATAGT TATGTTAGTA TTAAGAACGT TATTTATATT TCAAATTTTT CTTTTTTTTC
     1141 TGTACAGACG CGTGTACGCA TGTAACATTA TACTGAAAAC CTTGCTTGAG AAGGTTTTGG
     1201 GACGCTCGAA GGCTTTAATT TGCAAGCTCG CTGCGTCTTC GAATTCGCGC GTGATCTTAC
     1261 GGCATTATAC GTATGATCGG TCCACGATCA GCTAGATTAT CTAGTCAGCT TGATGTCATA
     1321 GCTGTTTCCT GAGGCTCAAT ACTGACCATT TAAATCATAC CTGACCTCCA TAGCAGAAAG
     1381 TCAAAAGCCT CCGACCGGAG GCTTTTGACT TGATCGGCAC GTAAGAGGTT CCAACTTTCA
     1441 CCATAATGAA ATAAGATCAC TACCGGGCGT ATTTTTTGAG TTATCGAGAT TTTCAGGAGC
     1501 TAAGGAAGCT AAAATGAGCC ATATTCAACG GGAAACGTCT TGCTTGAAGC CGCGATTAAA
     1561 TTCCAACATG GATGCTGATT TATATGGGTA TAAATGGGCT CGCGATAATG TCGGGCAATC
     1621 AGGTGCGACA ATCTATCGAT TGTATGGGAA GCCCGATGCG CCAGAGTTGT TTCTGAAACA
     1681 TGGCAAAGGT AGCGTTGCCA ATGATGTTAC AGATGAGATG GTCAGGCTAA ACTGGCTGAC
     1741 GGAATTTATG CCTCTTCCGA CCATCAAGCA TTTTATCCGT ACTCCTGATG ATGCATGGTT
     1801 ACTCACCACT GCGATCCCAG GGAAAACAGC ATTCCAGGTA TTAGAAGAAT ATCCTGATTC
     1861 AGGTGAAAAT ATTGTTGATG CGCTGGCAGT GTTCCTGCGC CGGTTGCATT CGATTCCTGT
     1921 TTGTAATTGT CCTTTTAACG GCGATCGCGT ATTTCGTCTC GCTCAGGCGC AATCACGAAT
     1981 GAATAACGGT TTGGTTGGTG CGAGTGATTT TGATGACGAG CGTAATGGCT GGCCTGTTGA
     2041 ACAAGTCTGG AAAGAAATGC ATAAACTCTT GCCATTCTCA CCGGATTCAG TCGTCACTCA
     2101 TGGTGATTTC TCACTTGATA ACCTTATTTT TGACGAGGGG AAATTAATAG GTTGTATTGA
     2161 TGTTGGACGA GTCGGAATCG CAGACCGATA CCAGGATCTT GCCATCCTAT GGAAC
//
