LOCUS       022_BB1_34_TDH1tt      2140 bp    DNA     circular UNA 07-Jan-2015
DEFINITION  
FEATURES             Location/Qualifiers
     CDS             join(1439..2140,1..117)
                     /label=KanR
     oriT            163..778
                     /label=ORI
     Primer          805..822
                     /label=Seq_BB1_FW
     Fusion_Site     948..951
                     /label=FS3
     Terminator      <952..>1153
                     /origin="Fragment (FR839629_Chr2 (TDH1(pGAP)TT_FW -
                     TDH1(pGAP)TT_RV)) (232bp linear)Fragment: from BsaI cut
                     at 11^12 to BsaI cut at 217^218, 206bpOrientation:
                     reverse complementedPlus strand 5':   no changePlus
                     strand 3':   no changeMinus strand 5':  no changeMinus
                     strand 3':  no change"
                     /label=tdh1TT
     Fusion_Site     1154..1157
                     /label=FS4
     Primer          complement(1240..1256)
                     /label=Seq_BB1_BW
ORIGIN
        1 TGCCTCGGTG AGTTTTCTCC TTCATTACAG AAACGGCTTT TTCAAAAATA TGGTATTGAT
       61 AATCCTGATA TGAATAAATT GCAGTTTCAC TTGATGCTCG ATGAGTTTTT CTAATGAGGA
      121 CCTAAATGTA ATCACCTGGC TCACCTTCGG GTGGGCCTTT CTGCGTTGCT GGCGTTTTTC
      181 CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAT GCTCAAGTCA GAGGTGGCGA
      241 AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT
      301 CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG
      361 GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG
      421 CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT
      481 CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC
      541 AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC
      601 TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTCG
      661 GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT
      721 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATTT
      781 TCTACCGAAG AAAGGCCCAC CCGTGTAAAA CGACGGCCAG TTTATCTAGT CAGCTTGATT
      841 CTAGCTGATC GTGGACCGGA AGGTGAGCCA GTGAGTTGAT TGCAGTCCAG TTACGCTGGA
      901 GTCTGAGGCT CGTCCTGAAT GATATGCGGC CTCGAATTCG AAGACGCGCT TTCGATTTGT
      961 ATGTGAAATA GCTGAAATTC GAAAATTTCA TTATGGCTGT ATCTACTTTA GCGTATTAGG
     1021 CATTTGAGCA TTGGCTTGAA CAATGCGGGC TGTAGTGTGT CACCAAAGAA ACCATTCGGG
     1081 TTCGGATCTG GAAGTCCTCA TCACGTGATG CCGATCTCGT GTATTTTATT TTCAGATAAC
     1141 ACCTGAGGAC TTTCGCTGCG TCTTCGAATT CGCGCGTGAT CTTACGGCAT TATACGTATG
     1201 ATCGGTCCAC GATCAGCTAG ATTATCTAGT CAGCTTGATG TCATAGCTGT TTCCTGAGGC
     1261 TCAATACTGA CCATTTAAAT CATACCTGAC CTCCATAGCA GAAAGTCAAA AGCCTCCGAC
     1321 CGGAGGCTTT TGACTTGATC GGCACGTAAG AGGTTCCAAC TTTCACCATA ATGAAATAAG
     1381 ATCACTACCG GGCGTATTTT TTGAGTTATC GAGATTTTCA GGAGCTAAGG AAGCTAAAAT
     1441 GAGCCATATT CAACGGGAAA CGTCTTGCTT GAAGCCGCGA TTAAATTCCA ACATGGATGC
     1501 TGATTTATAT GGGTATAAAT GGGCTCGCGA TAATGTCGGG CAATCAGGTG CGACAATCTA
     1561 TCGATTGTAT GGGAAGCCCG ATGCGCCAGA GTTGTTTCTG AAACATGGCA AAGGTAGCGT
     1621 TGCCAATGAT GTTACAGATG AGATGGTCAG GCTAAACTGG CTGACGGAAT TTATGCCTCT
     1681 TCCGACCATC AAGCATTTTA TCCGTACTCC TGATGATGCA TGGTTACTCA CCACTGCGAT
     1741 CCCAGGGAAA ACAGCATTCC AGGTATTAGA AGAATATCCT GATTCAGGTG AAAATATTGT
     1801 TGATGCGCTG GCAGTGTTCC TGCGCCGGTT GCATTCGATT CCTGTTTGTA ATTGTCCTTT
     1861 TAACGGCGAT CGCGTATTTC GTCTCGCTCA GGCGCAATCA CGAATGAATA ACGGTTTGGT
     1921 TGGTGCGAGT GATTTTGATG ACGAGCGTAA TGGCTGGCCT GTTGAACAAG TCTGGAAAGA
     1981 AATGCATAAA CTCTTGCCAT TCTCACCGGA TTCAGTCGTC ACTCATGGTG ATTTCTCACT
     2041 TGATAACCTT ATTTTTGACG AGGGGAAATT AATAGGTTGT ATTGATGTTG GACGAGTCGG
     2101 AATCGCAGAC CGATACCAGG ATCTTGCCAT CCTATGGAAC
//
