LOCUS       014_BB1_34_RPP1BTT      2437 bp    DNA     circular UNA 07-Jan-2015
DEFINITION  
FEATURES             Location/Qualifiers
     CDS             join(1736..2437,1..117)
                     /label=KanR
     oriT            163..778
                     /label=ORI
     Primer          805..822
                     /label=Seq_BB1_FW
     Fusion_Site     948..951
                     /label=FS3
     Terminator      952..>1450
                     /note="Forward primer name: RPP1BTT_M2_FW"
                     /note="Forward primer: gacaggtctccgCTTCGTTACCGATAATTGGT"
                     /note="Forward primer misprimes: 12"
                     /note="Reverse primer name: RPP1BTT_RV"
                     /note="Reverse primer:
                     GaTCggtcTcCagctGCTCCTCTAGATACCATCAA"
                     /note="Reverse primer misprimes: 10"
                     /note="Fragment length: 265"
                     /label=rpp1bTT
     Fusion_Site     1451..1454
                     /label=FS4
     Primer          complement(1537..1553)
                     /label=Seq_BB1_BW
ORIGIN
        1 TGCCTCGGTG AGTTTTCTCC TTCATTACAG AAACGGCTTT TTCAAAAATA TGGTATTGAT
       61 AATCCTGATA TGAATAAATT GCAGTTTCAC TTGATGCTCG ATGAGTTTTT CTAATGAGGA
      121 CCTAAATGTA ATCACCTGGC TCACCTTCGG GTGGGCCTTT CTGCGTTGCT GGCGTTTTTC
      181 CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAT GCTCAAGTCA GAGGTGGCGA
      241 AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT
      301 CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG
      361 GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG
      421 CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT
      481 CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC
      541 AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC
      601 TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTCG
      661 GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT
      721 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATTT
      781 TCTACCGAAG AAAGGCCCAC CCGTGTAAAA CGACGGCCAG TTTATCTAGT CAGCTTGATT
      841 CTAGCTGATC GTGGACCGGA AGGTGAGCCA GTGAGTTGAT TGCAGTCCAG TTACGCTGGA
      901 GTCTGAGGCT CGTCCTGAAT GATATGCGGC CTCGAATTCG AAGACGCGCT TGGATGATAC
      961 TTTAATTGAT GCATGTGAAG AATTGAAGAA ACAGTAATCT AGTAAAATAT AAGAAAGTCC
     1021 TGAAAGTAGT TCATTTAGTA AGCTATGAAC CCAAGTTGGG GTAACCGATT TTTCAGGTAA
     1081 ACAGCTGTAG TGAGGAGACA TCTCAAATCG CCTGAGAAGA AAAATATCTA TGAGACAACT
     1141 ACAATGCGAA GAATGTGTTC AAATGCTCTA TTATTCACTA GTTTACCTAT TCAATGACCA
     1201 ACTCCTGGGA GGCTTCGTTA CCGATAATTG GTAACTTAAC TAGTACGTCC TTCATCAACT
     1261 CGTAGACAGT GAAAGTGACG GCTTGTCCCG GAGCAACACG CATGATACGC GGCGTGATAC
     1321 CTTTGTACAG AGCACGCATA CCTTCCTCTT GGATCAATCT CTTGGAAATA CGAAGAATAC
     1381 GGGCCACACC AGACTCATTG GTGACACCAG TCTCTCTCTG CATACGAGTC TTGATGGTAT
     1441 CTAGAGGAGC CGCTGCGTCT TCGAATTCGC GCGTGATCTT ACGGCATTAT ACGTATGATC
     1501 GGTCCACGAT CAGCTAGATT ATCTAGTCAG CTTGATGTCA TAGCTGTTTC CTGAGGCTCA
     1561 ATACTGACCA TTTAAATCAT ACCTGACCTC CATAGCAGAA AGTCAAAAGC CTCCGACCGG
     1621 AGGCTTTTGA CTTGATCGGC ACGTAAGAGG TTCCAACTTT CACCATAATG AAATAAGATC
     1681 ACTACCGGGC GTATTTTTTG AGTTATCGAG ATTTTCAGGA GCTAAGGAAG CTAAAATGAG
     1741 CCATATTCAA CGGGAAACGT CTTGCTTGAA GCCGCGATTA AATTCCAACA TGGATGCTGA
     1801 TTTATATGGG TATAAATGGG CTCGCGATAA TGTCGGGCAA TCAGGTGCGA CAATCTATCG
     1861 ATTGTATGGG AAGCCCGATG CGCCAGAGTT GTTTCTGAAA CATGGCAAAG GTAGCGTTGC
     1921 CAATGATGTT ACAGATGAGA TGGTCAGGCT AAACTGGCTG ACGGAATTTA TGCCTCTTCC
     1981 GACCATCAAG CATTTTATCC GTACTCCTGA TGATGCATGG TTACTCACCA CTGCGATCCC
     2041 AGGGAAAACA GCATTCCAGG TATTAGAAGA ATATCCTGAT TCAGGTGAAA ATATTGTTGA
     2101 TGCGCTGGCA GTGTTCCTGC GCCGGTTGCA TTCGATTCCT GTTTGTAATT GTCCTTTTAA
     2161 CGGCGATCGC GTATTTCGTC TCGCTCAGGC GCAATCACGA ATGAATAACG GTTTGGTTGG
     2221 TGCGAGTGAT TTTGATGACG AGCGTAATGG CTGGCCTGTT GAACAAGTCT GGAAAGAAAT
     2281 GCATAAACTC TTGCCATTCT CACCGGATTC AGTCGTCACT CATGGTGATT TCTCACTTGA
     2341 TAACCTTATT TTTGACGAGG GGAAATTAAT AGGTTGTATT GATGTTGGAC GAGTCGGAAT
     2401 CGCAGACCGA TACCAGGATC TTGCCATCCT ATGGAAC
//
