LOCUS       021_BB1_34_PAS_chr4_0883      2413 bp    DNA     circular UNA 07-Jan-2015
DEFINITION  
FEATURES             Location/Qualifiers
     CDS             join(1712..2413,1..117)
                     /label=KanR
     oriT            163..778
                     /label=ORI
     Primer          805..822
                     /label=Seq_BB1_FW
     Fusion_Site     948..951
                     /label=FS3
     Terminator      join(<952..974,complement(975..1406),complement(1407..>1426))
                     /note="Forward primer name: PAS_chr4_0883 TT_rv_fs4"
                     /note="Forward primer:
                     gtaggGtctCtAGCgAAACAACCTGGTAAGAAACG"
                     /note="Forward primer misprimes: 10"
                     /note="Reverse primer name: PAS_chr4_0883 TT_FW_fs3"
                     /note="Reverse primer:
                     gaCaggtctcCgcttCTGTTTGTCCTTATACATCATTC"
                     /note="Reverse primer misprimes: 13"
                     /note="Fragment length: 505"
                     /label="PAS_chr4_0883 TT"
     Fusion_Site     1427..1430
                     /label=FS4
     Primer          complement(1513..1529)
                     /label=Seq_BB1_BW
ORIGIN
        1 TGCCTCGGTG AGTTTTCTCC TTCATTACAG AAACGGCTTT TTCAAAAATA TGGTATTGAT
       61 AATCCTGATA TGAATAAATT GCAGTTTCAC TTGATGCTCG ATGAGTTTTT CTAATGAGGA
      121 CCTAAATGTA ATCACCTGGC TCACCTTCGG GTGGGCCTTT CTGCGTTGCT GGCGTTTTTC
      181 CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAT GCTCAAGTCA GAGGTGGCGA
      241 AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT
      301 CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG
      361 GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG
      421 CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT
      481 CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC
      541 AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC
      601 TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTCG
      661 GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT
      721 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATTT
      781 TCTACCGAAG AAAGGCCCAC CCGTGTAAAA CGACGGCCAG TTTATCTAGT CAGCTTGATT
      841 CTAGCTGATC GTGGACCGGA AGGTGAGCCA GTGAGTTGAT TGCAGTCCAG TTACGCTGGA
      901 GTCTGAGGCT CGTCCTGAAT GATATGCGGC CTCGAATTCG AAGACGCGCT TCTGTTTGTC
      961 CTTATACATC ATTCCAAGGT TAGAAAAGGC CGGAAAACAG ACATTTTTAT TTCATAGAAT
     1021 ATTGCAGTAG GATATATCCG CTACACTCGC TTATCAGCCC ATTTTAATTT AATATTTTAG
     1081 TTTAATTTTT TAACTGATCA GTTAGCCTTC TTTCAGCAAG GGCTGAAAGA TGTCGTGCGT
     1141 AAACTCAAAT AGTTACTTAC TTTAGCAATG ATCATTTGTT TGCTCTTGTT GTAGCCAAGT
     1201 TTCTCGGCAG ACAAAAGCCG CTCGTTGAAG TCCATTCGGA AAGTCAAAGG TCATCGAAAG
     1261 TATTTCGAAG GGGCTGAATC TCGAAGGGGG CTGAATCTGC AGGGACTTTA CGTGTCTTAT
     1321 CGATCAAAAG ATTAATTCTA AAACAGGCAA GAAATGCGCG AATTAATCTT ACAACTATAG
     1381 TTGCTGCTGC TTTTTAGAGC GGTCAACGTT TCTTACCAGG TTGTTTCGCT GCGTCTTCGA
     1441 ATTCGCGCGT GATCTTACGG CATTATACGT ATGATCGGTC CACGATCAGC TAGATTATCT
     1501 AGTCAGCTTG ATGTCATAGC TGTTTCCTGA GGCTCAATAC TGACCATTTA AATCATACCT
     1561 GACCTCCATA GCAGAAAGTC AAAAGCCTCC GACCGGAGGC TTTTGACTTG ATCGGCACGT
     1621 AAGAGGTTCC AACTTTCACC ATAATGAAAT AAGATCACTA CCGGGCGTAT TTTTTGAGTT
     1681 ATCGAGATTT TCAGGAGCTA AGGAAGCTAA AATGAGCCAT ATTCAACGGG AAACGTCTTG
     1741 CTTGAAGCCG CGATTAAATT CCAACATGGA TGCTGATTTA TATGGGTATA AATGGGCTCG
     1801 CGATAATGTC GGGCAATCAG GTGCGACAAT CTATCGATTG TATGGGAAGC CCGATGCGCC
     1861 AGAGTTGTTT CTGAAACATG GCAAAGGTAG CGTTGCCAAT GATGTTACAG ATGAGATGGT
     1921 CAGGCTAAAC TGGCTGACGG AATTTATGCC TCTTCCGACC ATCAAGCATT TTATCCGTAC
     1981 TCCTGATGAT GCATGGTTAC TCACCACTGC GATCCCAGGG AAAACAGCAT TCCAGGTATT
     2041 AGAAGAATAT CCTGATTCAG GTGAAAATAT TGTTGATGCG CTGGCAGTGT TCCTGCGCCG
     2101 GTTGCATTCG ATTCCTGTTT GTAATTGTCC TTTTAACGGC GATCGCGTAT TTCGTCTCGC
     2161 TCAGGCGCAA TCACGAATGA ATAACGGTTT GGTTGGTGCG AGTGATTTTG ATGACGAGCG
     2221 TAATGGCTGG CCTGTTGAAC AAGTCTGGAA AGAAATGCAT AAACTCTTGC CATTCTCACC
     2281 GGATTCAGTC GTCACTCATG GTGATTTCTC ACTTGATAAC CTTATTTTTG ACGAGGGGAA
     2341 ATTAATAGGT TGTATTGATG TTGGACGAGT CGGAATCGCA GACCGATACC AGGATCTTGC
     2401 CATCCTATGG AAC
//
