LOCUS       015_BB1_34_RPS25Att      2423 bp    DNA     circular UNA 07-Jan-2015
DEFINITION  
FEATURES             Location/Qualifiers
     CDS             join(1722..2423,1..117)
                     /label=KanR
     oriT            163..778
                     /label=ORI
     Primer          805..822
                     /label=Seq_BB1_FW
     Fusion_Site     948..951
                     /label=FS3
     Terminator      952..>1436
                     /note="Forward primer name: RPS25ATT_M1_FW"
                     /note="Forward primer: GacagGtcTccgCTTCCTTGTCGAGTGTGTTT"
                     /note="Forward primer misprimes: 9"
                     /note="Reverse primer name: RPS25ATT_RV"
                     /note="Reverse primer:
                     gatcggtctCcAgctGTCAAATATTCAGATGGTGA"
                     /note="Reverse primer misprimes: 13"
                     /note="Fragment length: 458"
                     /label=rps25aTT
     Fusion_Site     1437..1440
                     /label=FS4
     Primer          complement(1523..1539)
                     /label=Seq_BB1_BW
ORIGIN
        1 TGCCTCGGTG AGTTTTCTCC TTCATTACAG AAACGGCTTT TTCAAAAATA TGGTATTGAT
       61 AATCCTGATA TGAATAAATT GCAGTTTCAC TTGATGCTCG ATGAGTTTTT CTAATGAGGA
      121 CCTAAATGTA ATCACCTGGC TCACCTTCGG GTGGGCCTTT CTGCGTTGCT GGCGTTTTTC
      181 CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAT GCTCAAGTCA GAGGTGGCGA
      241 AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT
      301 CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG
      361 GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG
      421 CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT
      481 CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC
      541 AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC
      601 TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTCG
      661 GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT
      721 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATTT
      781 TCTACCGAAG AAAGGCCCAC CCGTGTAAAA CGACGGCCAG TTTATCTAGT CAGCTTGATT
      841 CTAGCTGATC GTGGACCGGA AGGTGAGCCA GTGAGTTGAT TGCAGTCCAG TTACGCTGGA
      901 GTCTGAGGCT CGTCCTGAAT GATATGCGGC CTCGAATTCG AAGACGCGCT TTTAGTGTAC
      961 ATCTGATAAT ATAGTACTAC CACGTATGAT AATGTAGAGA ATAGGCTTCC TTGTCGAGTG
     1021 TGTTTGCAGT TTTCTTGAGT TTCAAGGTTT AAATGCTGGT ATATTAGTTC ATCGAAGGTT
     1081 TCAGCCAATA GCACCTTAAA TCAATCAAAC TAATTCGACT CTTACGAAAG AGCCTACTGT
     1141 GTTTAGTATC GAAGTCGTTT ACCTTTCATG TTGAATAGCT TCCTCTCTGA CCCTAACATT
     1201 TCAAGATCCT CCTAAAGTTA CCCGGATTGT GAAATTCTAA TGATCCACCT GCCCAATGCA
     1261 TTTTTTCTTT ATTCAGTTTA CCTTTTTTAC CTAATATACG AGCTTGTTAA AGTAAGTGGC
     1321 ACTGCAATAC TAGGCTTATT GTTGATATTA TGATGAATCG TTTTCACAAA CTTGATTTCC
     1381 TGTGAACTCA CCATGTACTA AGGAAAAAAA CATGCATCAC CATCTGAATA TTTGACCGCT
     1441 GCGTCTTCGA ATTCGCGCGT GATCTTACGG CATTATACGT ATGATCGGTC CACGATCAGC
     1501 TAGATTATCT AGTCAGCTTG ATGTCATAGC TGTTTCCTGA GGCTCAATAC TGACCATTTA
     1561 AATCATACCT GACCTCCATA GCAGAAAGTC AAAAGCCTCC GACCGGAGGC TTTTGACTTG
     1621 ATCGGCACGT AAGAGGTTCC AACTTTCACC ATAATGAAAT AAGATCACTA CCGGGCGTAT
     1681 TTTTTGAGTT ATCGAGATTT TCAGGAGCTA AGGAAGCTAA AATGAGCCAT ATTCAACGGG
     1741 AAACGTCTTG CTTGAAGCCG CGATTAAATT CCAACATGGA TGCTGATTTA TATGGGTATA
     1801 AATGGGCTCG CGATAATGTC GGGCAATCAG GTGCGACAAT CTATCGATTG TATGGGAAGC
     1861 CCGATGCGCC AGAGTTGTTT CTGAAACATG GCAAAGGTAG CGTTGCCAAT GATGTTACAG
     1921 ATGAGATGGT CAGGCTAAAC TGGCTGACGG AATTTATGCC TCTTCCGACC ATCAAGCATT
     1981 TTATCCGTAC TCCTGATGAT GCATGGTTAC TCACCACTGC GATCCCAGGG AAAACAGCAT
     2041 TCCAGGTATT AGAAGAATAT CCTGATTCAG GTGAAAATAT TGTTGATGCG CTGGCAGTGT
     2101 TCCTGCGCCG GTTGCATTCG ATTCCTGTTT GTAATTGTCC TTTTAACGGC GATCGCGTAT
     2161 TTCGTCTCGC TCAGGCGCAA TCACGAATGA ATAACGGTTT GGTTGGTGCG AGTGATTTTG
     2221 ATGACGAGCG TAATGGCTGG CCTGTTGAAC AAGTCTGGAA AGAAATGCAT AAACTCTTGC
     2281 CATTCTCACC GGATTCAGTC GTCACTCATG GTGATTTCTC ACTTGATAAC CTTATTTTTG
     2341 ACGAGGGGAA ATTAATAGGT TGTATTGATG TTGGACGAGT CGGAATCGCA GACCGATACC
     2401 AGGATCTTGC CATCCTATGG AAC
//
