LOCUS       Genomically_integrated_ 1196 bp ds-DNA     linear       18-DEC-2017
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..1196
                     /label="Reporter region for genome integration"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    complement(776..810)
                     /label="pR*G-1TAG-R"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    184..184
                     /label="c to a mutation in v5"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     CDS             1071..1088
                     /label="6x His Tag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    102..329
                     /label="Ubiquitin"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     gene            351..1091
                     /label="sfGFP"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1128..1196
                     /label="rrnB.T1_terminator"
                     /ApEinfo_revcolor=#7da958
                     /ApEinfo_fwdcolor=#7da958
     misc_feature    330..350
                     /label="N-degron"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1..74
                     /label="pLtetO_promoter"
                     /ApEinfo_revcolor=#7da958
                     /ApEinfo_fwdcolor=#7da958
     misc_feature    102..350
                     /label="Ubiquitin+N-degron"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcacatcagc
       61 aggacgcact gaccgaattc attaaagagg agaaaggtac cATGCAGATT TTTGTGAAGA
      121 CTTTAACAGG TAAGACGATT ACCCTGGAGG TGGAGTCCTC GGACACCATC GATAATGTAA
      181 AATCAAAAAT CCAAGATAAG GAAGGAATCC CTCCAGACCA GCAACGTCTG ATTTTCGCAG
      241 GTAAACAACT GGAGGATGGT CGCACGCTTT CGGACTACAA CATCCAGAAA GAATCTACCC
      301 TTCATTTGGT TCTGCGTCTG CGTGGAGGAT aGTTGTTTGT GCAGGAGCTT gcatccaagg
      361 gcgaggagct ctttactggc gtagtaccaa ttctcgtaga gctcgatggc gatgtaaatg
      421 gccataagtt ttccgtacgc ggcgagggcg agggcgatgc aactaacggc aagctcactc
      481 tcaagtttat ttgtactact ggcaagctcc cagtaccatg gccaactctc gtaactactc
      541 tgacctatgg cgtacaatgt ttttcccgct atccagatca catgaagcaa catgattttt
      601 ttaagtccgc aatgccagag ggctatgtac aagagcgcac tattagcttt aaggatgatg
      661 gcacctataa gactcgcgca gaggtaaagt ttgagggcga tactctcgta aatcgcattg
      721 agctcaaggg cattgatttt aaggaggatg gcaatattct cggccataag ctggagtata
      781 atttcaattc ccataatgta tacattaccg cagataagca aaagaatggc attaaggcga
      841 attttaagat tcgccataat gtggaggatg gctccgtaca actcgcagat cattatcaac
      901 aaaatactcc aattggcgat ggcccagtac tcctcccaga taatcattat ctctccactc
      961 aatccgtgct ctccaaagat ccaaatgaga agcgcgatca catggtactc ctggagtttg
     1021 taactgcagc aggcattact catggcatgg atgagctcta taagctcgag caccaccacc
     1081 accaccacta aaagcttgat gggggatccc atggtacgcg tgctagaggc atcaaataaa
     1141 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtga
//