LOCUS       pCML_221                3044 bp ds-DNA     circular     09-JUN-2017
SOURCE      
  ORGANISM  
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    855..875
                     /vntifkey="21"
                     /label=Linker\Sequence
                     /ApEinfo_fwdcolor=#808080
                     /ApEinfo_revcolor=#808080
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1134..1163
                     /vntifkey="21"
                     /label=c-myc\Tag
                     /ApEinfo_fwdcolor=#b9b900
                     /ApEinfo_revcolor=#b9b900
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             876..1127
                     /vntifkey="4"
                     /label=YFP\C\terminus
                     /ApEinfo_fwdcolor=#ff80ff
                     /ApEinfo_revcolor=#ff80ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     831..839
                     /label=M13-rev
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     819..830
                     /label=M13-rev(1)
                     /ApEinfo_label=M13-rev
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_binding    791..813
                     /label=LacO
                     /ApEinfo_fwdcolor=#6495ed
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     819..839
                     /label=M13-rev(2)
                     /ApEinfo_label=M13-rev
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1659..2454
                     /label=KanR
                     /ApEinfo_fwdcolor=#ff8040
                     /ApEinfo_revcolor=#ff8040
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    757..786
                     /label=lac promoter
                     /ApEinfo_fwdcolor=#00ff40
                     /ApEinfo_revcolor=#ff8080
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 atcaattacc agtggctgct gccagtggtg cttttgcatg tctttccggg ttggactcaa
       61 gacgatagtt accggataag gcgcagcggt cggactgaac ggggggttcg tgcatacagt
      121 ccagcttgga gcgaactgcc tacccggaac tgagtgtcag gcgtggaatg agacaaacgc
      181 ggccataaca gcggaatgac accggtaaac cgaaaggcag gaacaggaga gcgcacgagg
      241 gagccgccag gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc caccactgat
      301 ttgagcgtca gatttcgtga tgcttgtcag gggggcggag cctatggaaa aacggctttg
      361 ccgcggccct ctcacttccc tgttaagtat cttcctggca tcttccagga aatctccgcc
      421 ccgttcgtaa gccatttccg ctcgccgcag tcgaacgacc gagcgtagcg agtcagtgag
      481 cgaggaagcg gaatatatcc tgtatcacat attctgctga cgcaccggtg cagccttttt
      541 tctcctgcca catgaagcac ttcactgaca ccctcatcag tgccaacata gtaagccagt
      601 atatacactc cgctagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
      661 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
      721 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
      781 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      841 ttacgccaag cttgaaacag aaagttatga atcatgacaa gcagaagaac ggcatcaagg
      901 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
      961 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct
     1021 accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     1081 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagact agtgaacaga
     1141 aactgattag cgaagaagat ctgtaaatcg atataactaa gtaatatggt gcactctcag
     1201 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     1261 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     1321 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     1381 ccgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgctgatgtc cggcggtgct
     1441 tttgccgtta cgcaccaccc cgtcagtagc tgaacaggag ggacaggggg tgggcgaaga
     1501 actccagcat gagatccccg cgctggagga tcatccagcc ggcgtcccgg aaaacgattc
     1561 cgaagcccaa cctttcatag aaggcggcgg tggaatcgaa atctcgtgat ggcaggttgg
     1621 gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct cagaagaact cgtcaagaag
     1681 gcgatagaag gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg
     1741 gtcagcccat tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg
     1801 atagcggtcc gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc
     1861 caccatgata ttcggcaagc aggcatcgcc atgggtcacg acgagatcct cgccgtcggg
     1921 catccgcgcc ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc
     1981 cagatcatcc tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg
     2041 tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc
     2101 atcagccatg atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc
     2161 cggcacttcg cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc
     2221 tgcgcaagga acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cttggagttc
     2281 attcagggca ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag
     2341 ccggaacacg gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag
     2401 cctctccacc caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa
     2461 cgatcctcat cctgtctctt gatcagatct tgatcccctg cgccatcaga tccttggcgg
     2521 caagaaagcc atccagttta ctttgcaggg cttcccaacc ttaccagagg gcgccccagc
     2581 tggcaattcc ggttcgcttg ctgtccataa aaccgcccag tctagctatc gccatgtaag
     2641 cccactgcaa gctacctgct ttctctttgc gcttgcgttt tcccttgtcc agatagccca
     2701 gtagctgaca ttcatccggg gtcagcaccg tttctgcgga ctggctttct acgtgttccg
     2761 cttcctttag cagcccttgc gccctgagtg cttgcggcag cgtgaagctg tgcctagaaa
     2821 tattttatct gattaataag atgatcttct tgagatcgtt ttggtctgcg cgtaatctct
     2881 tgctctgaaa acgaaaaaac cgccttgcag ggcggttttt cgaaggttct ctgagctacc
     2941 aactctttga accgaggtaa ctggcttgga ggagcgcagt caccaaaact tgtcctttca
     3001 gtttagcctt aaccggcgca tgacttcaag actaactcct ctaa 
//
