LOCUS       GG_iRS3_SS_annot        4850 bp ds-DNA     linear       07-JUN-2017
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    2978..2996
                     /label=CB Region
                     /ApEinfo_fwdcolor=#fce903
                     /ApEinfo_revcolor=#fce903
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1885..2971
                     /label=PheS cassette
                     /ApEinfo_fwdcolor=#8080ff
                     /ApEinfo_revcolor=#8080ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(1510..1792)
                     /label=pBAD
                     /ApEinfo_fwdcolor=#c0c0c0
                     /ApEinfo_revcolor=#c0c0c0
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1799..1872
                     /label=pLtetO
                     /ApEinfo_fwdcolor=#800040
                     /ApEinfo_revcolor=#008000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2997..3002
                     /label=Linker Region/Loop
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3008..3015
                     /label=RBS
                     /ApEinfo_fwdcolor=#d12ec1
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3024..3740
                     /label=GFP
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=#00ff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(1111..1503)
                     /label=GROUP I INTRON WILD TYPE
                     /ApEinfo_fwdcolor=#ff0080
                     /ApEinfo_revcolor=#ff0080
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(1..795)
                     /label=KanR
                     /ApEinfo_fwdcolor=#408080
                     /ApEinfo_revcolor=#408080
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4032..4714
                     /label=ColE1
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT
       61 ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG
      121 GGTAGCCAAC GCTATGTCCT GATAGCGGTC CGCCACACCC AGCCGGCCAC AGTCGATGAA
      181 TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC
      241 GACGAGATCC TCGCCGTCGG GCATGCGCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC
      301 GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT
      361 ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG
      421 CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG
      481 AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC
      541 AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG
      601 CGCTGCCTCG TCCTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC
      661 CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG
      721 TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC
      781 ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCAGATC TTGATCCCCT
      841 GCGCCATCAG ATCCTTGGCG GCAAGAAAGC CATCCAGTTT ACTTTGCAGG GCTTCCCAAC
      901 CTTACCAGAG GGCGCCCCAG CTGGCAATTC CGACGTCAAA GGGCCCAAGG CCCAGTCTTT
      961 CGACTGAGCC TTTCGTTTTA TTTGATGCCT GGCAGTTCCC TACTCTCGCA TGGGGAGACC
     1021 CCACACTACC ATCGGCGCTA CGGCGTTTCA CTTCTGAGTT CGGCATGGGG TCAGGTGGGA
     1081 CCACCGCGCT ACTGCCGCCA GGCATCTAGA CGAGTACTCC AAAACTAATC AATATACTTT
     1141 CGCATACAAA TTAGTTCCCA GCGGCTCCAG TGTTGCATCA CTTCATCCGC TAGCTCCCAT
     1201 TAAGGAGAGG TCCGACTATA TCTTATGAGA AGAATACATC TTCCCCGACC GACATTTAGT
     1261 CTGTGAACTG CATCCATATC AACAGAAGAT CTGTTGACTT AGGACTTGGC TGCGTGGTTA
     1321 GGACCATGTC CGTCAGCTTA TTACCATACC CTTTGCAAGG CCATCTCAAA GTTTCCCCTG
     1381 AGACTTGGTA CTGAACGGCT GTTGACCCCT TTCCCGCAAT TTGACGGTCT TGCCTTTTAA
     1441 ACCGATGCAA TCTATTGGTT TAAAGACTAG CTACCAGGTG CATGCCTGAT AACTTTTCCC
     1501 TCCGTCGACT ATGGAGAAAC AGTAGAGAGT TGCGATAAAA AGCGTCAGGT AGGATCCGCT
     1561 AATCTTATGG ATAAAAATGC TATGGCATAG CAAAGTGTGA CGCCGTGCAA ATAATCAATG
     1621 TGGACTTTTC TGCCGTGATT ATAGACACTT TTGTTACGCG TTTTTGTCAT GGCTTTGGTC
     1681 CCGCTTTGTT ACAGAATGCT TTTAATAAGC GGGGTTACCG GTTTGGTTAG CGAGAAGAGC
     1741 CAGTAAAAGA CGCAGTGACG GCAATGTCTG ATGCAATATG GACAATTGGT TTCTCGAGTC
     1801 CCTATCAGTG ATAGAGATTG ACATCCCTAT CAGTGATAGA GATACTGAGC ACATCAGCAG
     1861 GACGCACTGA CCGAATTCGA GACGAGAAAA GCAGAAAAAA CGCCGCTGAA CGCGGCGTTT
     1921 TGGATTGTTT GCTGTTTCTt tatttaaact gtttgaggaa acgcagatcg ttttcgaaga
     1981 atgaacgcag gtcggtgacg ccgtaacgca acatagtcag acgctccatc cccatcccga
     2041 agccgaaacc agagtaaact tccgggtcga tgccaacgtt acgcaacacg ttcggatgca
     2101 ccatcccgca gcccagcact tccagccatt taccgttttt acccatgacg tccacttctg
     2161 cagaaggttc ggtaaacggg aagtaggaag gacggaagcg aatctgcaaa tcttcctcaa
     2221 agaagttacg caggaagtcg tgcagcgtgc ctttcaggtt ggtaaagctg atgttggtat
     2281 caacaatcag accttccatc tgatggaaca tcggcgtgtg agtctggtcg tagtcgttac
     2341 gataaacacg gccaggcgcg atgatacgaa tcggtggctg ctgggctttc atggtgcgga
     2401 tctgtacgcc agaggtctgg gtacgcagca ggcgggtagt gtcaaaccag aaagtgtcgt
     2461 ggtcagcgcg cgccgggtgg tgaccaggaa tgttcagagc atcgaagtta tgataatcgt
     2521 cttcgatttc cggcccggtt gccacggtaa agccaagctc accgaagaaa ctttcgatac
     2581 ggtcgatggt acgggtaacc ggatgcagac cgccgttttc aatgcgacga cctggcagag
     2641 agacatcaat cgtttccgcc gccagacgcg cattcagtgc agcgctttcc agttccgctt
     2701 tacgcgcatt cagcgcctgc tgaacctgct ctttcgcttc gttgataacc gcaccagctg
     2761 ccggacgctc ttctggcggc agctcacgca gggtcgtcat ctgaagggtt aagtgccctt
     2821 ttttacccaa atattcgacg cgcacattat ctaacgcggc aacatctgac gcctggctaa
     2881 tggccgcctt cgcactggca accagttctg cgagatgtga catCTAGTAT TTCCCCTCTT
     2941 TCAAAGCGTA AACAACGTAT AACGGCGTAT GCGTCTCTAC CATTCACCTC TTGGATTTGG
     3001 GTATTAAAGA GGAGAAAGGT ACCATGAGTA AAGGAGAAGA ACTTTTCACT GGAGTTGTCC
     3061 CAATTCTTGT TGAATTAGAT GGTGATGTTA ATGGGCACAA ATTTTCTGTC AGTGGAGAGG
     3121 GTGAAGGTGA TGCAACATAC GGAAAACTTA CCCTTAAATT TATTTGCACT ACTGGAAAAC
     3181 TACCTGTTCC ATGGCCAACA CTTGTCACTA CTTTCGGTTA TGGTGTTCAA TGCTTTGCGA
     3241 GATACCCAGA TCATATGAAA CAGCATGACT TTTTCAAGAG TGCCATGCCC GAAGGTTATG
     3301 TACAGGAAAG AACTATATTT TTCAAAGATG ACGGGAACTA CAAGACACGT GCTGAAGTCA
     3361 AGTTTGAAGG TGATACCCTT GTTAATAGAA TCGAGTTAAA AGGTATTGAT TTTAAAGAAG
     3421 ATGGAAACAT TCTTGGACAC AAATTGGAAT ACAACTATAA CTCACACAAT GTATACATCA
     3481 TGGCAGACAA ACAAAAGAAT GGAATCAAAG TTAACTTCAA AATTAGACAC AACATTGAAG
     3541 ATGGAAGCGT TCAACTAGCA GACCATTATC AACAAAATAC TCCAATTGGC GATGGCCCTG
     3601 TCCTTTTACC AGACAACCAT TACCTGTCCA CACAATCTGC CCTTTCGAAA GATCCCAACG
     3661 AAAAGAGAGA CCACATGGTC CTTCTTGAGT TTGTAACAGC TGCTGGGATT ACACATGGCA
     3721 TGGATGATCT CTACAAATAA AAGCTTGATA TCGAATTCCT GCAGCCCGGG GGATCCCATG
     3781 GTACGCGTGC TAGAGGCATC AAATAAAACG AAAGGCTCAG TCGAAAGACT GGGCCTTTCG
     3841 TTTTATCTGT TGTTTGTCGG TGAACGCTCT CCTGAGTAGG ACAAATCCGC CNCCNTAGAC
     3901 CTGGGCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC
     3961 ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG
     4021 AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT
     4081 CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG
     4141 GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA
     4201 TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG
     4261 TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT
     4321 CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC
     4381 GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC
     4441 GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT
     4501 GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC
     4561 GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC
     4621 AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG
     4681 AACGAAAACT CACGTTAAGG GATTTTGGTC ATGACTAGTG CTTGGATTCT CACCAATAAA
     4741 AAACGCCCGG CGGCAACCGA GCGTTCTGAA CAAATCCAGA TGGAGTTCTG AGGTCATTAC
     4801 TGGATCTATC AACAGGAGTC CAAGCGAGCT CTCGAACCCC AGAGTCCCGC 
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