LOCUS       pAAV_DIO_hChR2-m        7158 bp ss-DNA     linear   SYN 07-Jun-2017
DEFINITION  -
ACCESSION   -
KEYWORDS    -
SOURCE      -
FEATURES             Location/Qualifiers
     source          join(<2582..>2585,<2582..>2585)
                     /db_xref="gi:1419022"
                     /note="GenBank# X95710 [Split]"
     source          <2582..>2585
                     /db_xref="taxon:10345"
                     /note="virion [Split]"
                     /organism="Pseudorabies virus"
     source          <2582..>2585
                     /db_xref="gi:61413"
                     /note="GenBank# X55001 [Split]"
     misc_feature    1..141
                     /note="ITR"
     intron          complement(<1587..1611)
                     /note="Chimeric intron [Split]"
     misc_feature    complement(1606..1611)
                     /note="Splice donor"
     CDS             complement(1612..1626)
                     /note="Linker"
     misc_feature    join(<1612..1625,complement(<2586..2601))
                     /note="ZK106 [Split]"
     CDS             complement(1627..2343)
                     /note="EGFP sequence"
     misc_feature    1627..1652
                     /note="ZK107"
     primer_bind     complement(1669..1690)
                     /note="EGFP-C"
     misc_feature    complement(1730..1755)
                     /note="Sequencing Primer 102"
     primer_bind     complement(2320..>2343)
                     /note="oME298 [Split]"
     misc_feature    complement(2330..>2336)
                     /note="Bac recombineering primer - FWD [Split]"
     misc_feature    complement(2340..2349)
                     /note="Kozak"
     misc_feature    <2472..2477
                     /note="MfeI [Split]"
     intron          <2474..2585
                     /note="Chimeric intron [Split]"
     primer_bind     2478..2502
                     /note="oME130 (+MfeI)"
     misc_feature    2531..2537
                     /note="Branch site?"
     misc_feature    2554..2560
                     /note="Branch site?"
     misc_feature    2571..2585
                     /note="Splice acceptor"
     misc_feature    <2582..>2585
                     /note="Good site to insert intron [Split]"
     misc_feature    <2582..>2585
                     /note="Unique Long (UL) Sequence [Split]"
     CDS             2586..2609
                     /note="Linker"
     misc_feature    2597..2602
                     /note="Hind III preceding L10"
     misc_difference 2609
                     /note="C->T to remove EcoRI."
     misc_feature    2610..2634
                     /note="ZK109"
     CDS             2610..3260
                     /note="L10 sequence"
     misc_feature    complement(2660..2687)
                     /note="ZK89 genotyping primer"
     primer_bind     complement(2682..2703)
                     /note="oME327"
     misc_feature    2880..2885
                     /note="Hind III in the middle of L10"
     primer_bind     complement(3237..>3260)
                     /note="oME299 [Split]"
     misc_feature    complement(3239..>3260)
                     /note="ZK108 [Split]"
     misc_feature    3285..3873
                     /note="WPRE"
     misc_feature    4421..4561
                     /note="ITR"
BASE COUNT      1489 A      1885 C      1968 G      1816 T         0 OTHER
ORIGIN      ?
        1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCAAAG CCCGGGCGTC
       61 GGGCGACCTT TGGTCGCCCG GCCTCAGTGA GCGAGCGAGC GCGCAGAGAG GGAGTGGCCA
      121 ACTCCATCAC TAGGGGTTCC TGCGGCCGCA CGCGTAAGCT TTGCAAAGAT GGATAAAGTT
      181 TTAAACAGAG AGGAATCTTT GCAGCTAATG GACCTTCTAG GTCTTGAAAG GAGTGGGAAT
      241 TGGCTCCGGT GCCCGTCAGT GGGCAGAGCG CACATCGCCC ACAGTCCCCG AGAAGTTGGG
      301 GGGAGGGGTC GGCAATTGAA CCGGTGCCTA GAGAAGGTGG CGCGGGGTAA ACTGGGAAAG
      361 TGATGTCGTG TACTGGCTCC GCCTTTTTCC CGAGGGTGGG GGAGAACCGT ATATAAGTGC
      421 AGTAGTCGCC GTGAACGTTC TTTTTCGCAA CGGGTTTGCC GCCAGAACAC AGGTAAGTGC
      481 CGTGTGTGGT TCCCGCGGGC CTGGCCTCTT TACGGGTTAT GGCCCTTGCG TGCCTTGAAT
      541 TACTTCCACT GGCTGCAGTA CGTGATTCTT GATCCCGAGC TTCGGGTTGG AAGTGGGTGG
      601 GAGAGTTCGA GGCCTTGCGC TTAAGGAGCC CCTTCGCCTC GTGCTTGAGT TGAGGCCTGG
      661 CCTGGGCGCT GGGGCCGCCG CGTGCGAATC TGGTGGCACC TTCGCGCCTG TCTCGCTGCT
      721 TTCGATAAGT CTCTAGCCAT TTAAAATTTT TGATGACCTG CTGCGACGCT TTTTTTCTGG
      781 CAAGATAGTC TTGTAAATGC GGGCCAAGAT CTGCACACTG GTATTTCGGT TTTTGGGGCC
      841 GCGGGCGGCG ACGGGGCCCG TGCGTCCCAG CGCACATGTT CGGCGAGGCG GGGCCTGCGA
      901 GCGCGGCCAC CGAGAATCGG ACGGGGGTAG TCTCAAGCTG GCCGGCCTGC TCTGGTGCCT
      961 GGCCTCGCGC CGCCGTGTAT CGCCCCGCCC TGGGCGGCAA GGCTGGCCCG GTCGGCACCA
     1021 GTTGCGTGAG CGGAAAGATG GCCGCTTCCC GGCCCTGCTG CAGGGAGCTC AAAATGGAGG
     1081 ACGCGGCGCT CGGGAGAGCG GGCGGGTGAG TCACCCACAC AAAGGAAAAG GGCCTTTCCG
     1141 TCCTCAGCCG TCGCTTCATG TGACTCCACG GAGTACCGGG CGCCGTCCAG GCACCTCGAT
     1201 TAGTTCTCGA GCTTTTGGAG TACGTCGTCT TTAGGTTGGG GGGAGGGGTT TTATGCGATG
     1261 GAGTTTCCCC ACACTGAGTG GGTGGAGACT GAAGTTAGGC CAGCTTGGCA CTTGATGTAA
     1321 TTCTCCTTGG AATTTGCCCT TTTTGAGTTT GGATCTTGGT TCATTCTCAA GCCTCAGACA
     1381 GTGGTTCAAA GTTTTTTTCT TCCATTTCAG GTGTCGTGAG GTACCGGATC CTCTAGAGTC
     1441 GACTCCGGAA TAACTTCGTA TAGGATACTT TATACGAAGT TATGCAGAAT GGTAGCTGGA
     1501 TTGTAGCTGC TATTAGCAAT ATGAAACCTC TTAATAACTT CGTATAGCAT ACATTATACG
     1561 AAGTTATGGC GCGCCTACGC GGCCGCCCTG TCTTGTAACC TTGATACTTA CCTGAGTCCG
     1621 GCCGGACTTG TACAGCTCGT CCATGCCGAG AGTGATCCCG GCGGCGGTCA CGAACTCCAG
     1681 CAGGACCATG TGATCGCGCT TCTCGTTGGG GTCTTTGCTC AGGGCGGACT GGGTGCTCAG
     1741 GTAGTGGTTG TCGGGCAGCA GCACGGGGCC GTCGCCGATG GGGGTGTTCT GCTGGTAGTG
     1801 GTCGGCGAGC TGCACGCTGC CGTCCTCGAT GTTGTGGCGG ATCTTGAAGT TCACCTTGAT
     1861 GCCGTTCTTC TGCTTGTCGG CCATGATATA GACGTTGTGG CTGTTGTAGT TGTACTCCAG
     1921 CTTGTGCCCC AGGATGTTGC CGTCCTCCTT GAAGTCGATG CCCTTCAGCT CGATGCGGTT
     1981 CACCAGGGTG TCGCCCTCGA ACTTCACCTC GGCGCGGGTC TTGTAGTTGC CGTCGTCCTT
     2041 GAAGAAGATG GTGCGCTCCT GGACGTAGCC TTCGGGCATG GCGGACTTGA AGAAGTCGTG
     2101 CTGCTTCATG TGGTCGGGGT AGCGGCTGAA GCACTGCACG CCGTAGGTCA GGGTGGTCAC
     2161 GAGGGTGGGC CAGGGCACGG GCAGCTTGCC GGTGGTGCAG ATGAACTTCA GGGTCAGCTT
     2221 GCCGTAGGTG GCATCGCCCT CGCCCTCGCC GGACACGCTG AACTTGTGGC CGTTTACGTC
     2281 GCCGTCCAGC TCGACCAGGA TGGGCACCAC CCCGGTGAAC AGCTCCTCGC CCTTGCTCAC
     2341 CATGGTGGCT AGCATAACTT CGTATAAAGT ATCCTATACG AAGTTATTTG CCTTAACCCA
     2401 GAAATTATCA CTGTTATTCT TTAGAATGGT GCAAAGAATA ACTTCGTATA ATGTATGCTA
     2461 TACGAAGTTA TCAATTGTTT AAGGAGACCA ATAGAAACTG GGCTTGTCGA GACAGAGAAG
     2521 ACTCTTGCGT TTCTGATAGG CACCTATTGG TCTTACTGAC ATCCACTTTG CCTTTCTCTC
     2581 CACAGATCTC GAGCTCAAGC TTCGAATTTA GCAGCAAAGT CTCACGCGAC ACCCTGTACG
     2641 AGGCGGTGCG GGAAGTCCTG CACGGGAACC AGCGCAAGCG CCGCAAGTTT CTGGAGACGG
     2701 TGGAGCTGCA GATCAGCCTG AAGAACTACG ACCCTCAGAA GGACAAACGT TTCTCGGGCA
     2761 CCGTCAGGCT CAAGTCCACC CCACGCCCCA AGTTCTCGGT GTGCGTTCTG GGGGACCAGC
     2821 AGCACTGTGA TGAAGCCAAG GCCGTGGATA TCCCCCACAT GGACATCGAG GCGCTCAAGA
     2881 AGCTTAACAA AAACAAGAAG TTGGTCAAGA AGCTGGCTAA GAAGTACGAT GCCTTTTTGG
     2941 CCTCTGAGTC TCTGATTAAG CAGATCCCAC GTATCCTGGG CCCAGGCCTA AACAAGGCTG
     3001 GCAAGTTCCC CTCCCTGCTG ACACACAATG AAAACATGGT GGCCAAAGTG GATGAGGTGA
     3061 AATCGACAAT CAAGTTCCAG ATGAAGAAGG TGCTGTGTTT GGCCGTCGCT GTTGGCCACG
     3121 TGAAGATGAC CGATGATGAG CTAGTCTACA ACATTCATCT GGCTGTCAAT TTCTTGGTGT
     3181 CCTTGCTTAA GAAAAACTGG CAAAACGTGC GGGCTCTGTA CATCAAGAGC ACCATGGGCA
     3241 AGCCCCAGCG TCTGTATTAG GAATTCGATA TCAAGCTTAT CGATAATCAA CCTCTGGATT
     3301 ACAAAATTTG TGAAAGATTG ACTGGTATTC TTAACTATGT TGCTCCTTTT ACGCTATGTG
     3361 GATACGCTGC TTTAATGCCT TTGTATCATG CTATTGCTTC CCGTATGGCT TTCATTTTCT
     3421 CCTCCTTGTA TAAATCCTGG TTGCTGTCTC TTTATGAGGA GTTGTGGCCC GTTGTCAGGC
     3481 AACGTGGCGT GGTGTGCACT GTGTTTGCTG ACGCAACCCC CACTGGTTGG GGCATTGCCA
     3541 CCACCTGTCA GCTCCTTTCC GGGACTTTCG CTTTCCCCCT CCCTATTGCC ACGGCGGAAC
     3601 TCATCGCCGC CTGCCTTGCC CGCTGCTGGA CAGGGGCTCG GCTGTTGGGC ACTGACAATT
     3661 CCGTGGTGTT GTCGGGGAAA TCATCGTCCT TTCCTTGGCT GCTCGCCTAT GTTGCCACCT
     3721 GGATTCTGCG CGGGACGTCC TTCTGCTACG TCCCTTCGGC CCTCAATCCA GCGGACCTTC
     3781 CTTCCCGCGG CCTGCTGCCG GCTCTGCGGC CTCTTCCGCG TCTTCGCCTT CGCCCTCAGA
     3841 CGAGTCGGAT CTCCCTTTGG GCCGCCTCCC CGCATCGATA CCGAGCGCTG CTCGAGAGAT
     3901 CTACGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC
     3961 TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT
     4021 GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA
     4081 GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC
     4141 TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA
     4201 GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG
     4261 ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC
     4321 ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT
     4381 TCTGATTTTG TAGGTAACCA CGTGCGGACC GAGCGGCCGC AGGAACCCCT AGTGATGGAG
     4441 TTGGCCACTC CCTCTCTGCG CGCTCGCTCG CTCACTGAGG CCGGGCGACC AAAGGTCGCC
     4501 CGACGCCCGG GCTTTGCCCG GGCGGCCTCA GTGAGCGAGC GAGCGCGCAG CTGCCTGCAG
     4561 GGGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATACGT
     4621 CAAAGCAACC ATAGTACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA
     4681 CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC
     4741 CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT
     4801 TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT TTGGGTGATG
     4861 GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA
     4921 CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGGCT
     4981 ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA
     5041 TTTAACAAAA ATTTAACGCG AATTTTAACA AAATATTAAC GTTTACAATT TTATGGTGCA
     5101 CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GCCCCGACAC CCGCCAACAC
     5161 CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA
     5221 CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGAC
     5281 GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT
     5341 AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT
     5401 AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT
     5461 ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG
     5521 CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG
     5581 AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC
     5641 TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT
     5701 GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT
     5761 ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA
     5821 TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT
     5881 TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG
     5941 ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG
     6001 AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG
     6061 AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG
     6121 CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG
     6181 CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC
     6241 GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA
     6301 TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT
     6361 ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC
     6421 TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG
     6481 ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT
     6541 GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC
     6601 CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC
     6661 TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG
     6721 CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT
     6781 TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT
     6841 GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC
     6901 TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA
     6961 GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA
     7021 GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG
     7081 GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT
     7141 GGCCTTTTGC TCACATGT
//
