LOCUS       pAAV_DIO_hChR2-m        7008 bp ss-DNA     circular SYN 08-Jun-2017
DEFINITION  -
ACCESSION   -
KEYWORDS    -
SOURCE      -
FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /note="ITR"
     primer_bind     <1570..1599
                     /note="oME299 [Split]"
     misc_feature    <1572..1597
                     /note="ZK108 [Split]"
     CDS             complement(1576..2226)
                     /note="L10 sequence"
     misc_feature    complement(1951..1956)
                     /note="Hind III in the middle of L10"
     primer_bind     2133..2154
                     /note="oME327"
     misc_feature    2149..2176
                     /note="ZK89 genotyping primer"
     misc_feature    complement(2202..2226)
                     /note="ZK109"
     misc_feature    complement(2234..2239)
                     /note="Hind III preceding L10"
     misc_feature    2235..2264
                     /note="ZK106"
     CDS             complement(2266..2982)
                     /note="EGFP sequence"
     misc_feature    2266..2291
                     /note="ZK107"
     primer_bind     complement(2308..2329)
                     /note="EGFP-C"
     misc_feature    complement(2369..2394)
                     /note="Sequencing Primer 102"
     primer_bind     complement(2959..>2987)
                     /note="oME298 [Split]"
     misc_feature    complement(2969..>2975)
                     /note="Bac recombineering primer - FWD [Split]"
     misc_feature    3135..3723
                     /note="WPRE"
     misc_feature    4271..4411
                     /note="ITR"
BASE COUNT      1413 A      1851 C      1933 G      1811 T         0 OTHER
ORIGIN      ?
        1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCAAAG CCCGGGCGTC
       61 GGGCGACCTT TGGTCGCCCG GCCTCAGTGA GCGAGCGAGC GCGCAGAGAG GGAGTGGCCA
      121 ACTCCATCAC TAGGGGTTCC TGCGGCCGCA CGCGTAAGCT TTGCAAAGAT GGATAAAGTT
      181 TTAAACAGAG AGGAATCTTT GCAGCTAATG GACCTTCTAG GTCTTGAAAG GAGTGGGAAT
      241 TGGCTCCGGT GCCCGTCAGT GGGCAGAGCG CACATCGCCC ACAGTCCCCG AGAAGTTGGG
      301 GGGAGGGGTC GGCAATTGAA CCGGTGCCTA GAGAAGGTGG CGCGGGGTAA ACTGGGAAAG
      361 TGATGTCGTG TACTGGCTCC GCCTTTTTCC CGAGGGTGGG GGAGAACCGT ATATAAGTGC
      421 AGTAGTCGCC GTGAACGTTC TTTTTCGCAA CGGGTTTGCC GCCAGAACAC AGGTAAGTGC
      481 CGTGTGTGGT TCCCGCGGGC CTGGCCTCTT TACGGGTTAT GGCCCTTGCG TGCCTTGAAT
      541 TACTTCCACT GGCTGCAGTA CGTGATTCTT GATCCCGAGC TTCGGGTTGG AAGTGGGTGG
      601 GAGAGTTCGA GGCCTTGCGC TTAAGGAGCC CCTTCGCCTC GTGCTTGAGT TGAGGCCTGG
      661 CCTGGGCGCT GGGGCCGCCG CGTGCGAATC TGGTGGCACC TTCGCGCCTG TCTCGCTGCT
      721 TTCGATAAGT CTCTAGCCAT TTAAAATTTT TGATGACCTG CTGCGACGCT TTTTTTCTGG
      781 CAAGATAGTC TTGTAAATGC GGGCCAAGAT CTGCACACTG GTATTTCGGT TTTTGGGGCC
      841 GCGGGCGGCG ACGGGGCCCG TGCGTCCCAG CGCACATGTT CGGCGAGGCG GGGCCTGCGA
      901 GCGCGGCCAC CGAGAATCGG ACGGGGGTAG TCTCAAGCTG GCCGGCCTGC TCTGGTGCCT
      961 GGCCTCGCGC CGCCGTGTAT CGCCCCGCCC TGGGCGGCAA GGCTGGCCCG GTCGGCACCA
     1021 GTTGCGTGAG CGGAAAGATG GCCGCTTCCC GGCCCTGCTG CAGGGAGCTC AAAATGGAGG
     1081 ACGCGGCGCT CGGGAGAGCG GGCGGGTGAG TCACCCACAC AAAGGAAAAG GGCCTTTCCG
     1141 TCCTCAGCCG TCGCTTCATG TGACTCCACG GAGTACCGGG CGCCGTCCAG GCACCTCGAT
     1201 TAGTTCTCGA GCTTTTGGAG TACGTCGTCT TTAGGTTGGG GGGAGGGGTT TTATGCGATG
     1261 GAGTTTCCCC ACACTGAGTG GGTGGAGACT GAAGTTAGGC CAGCTTGGCA CTTGATGTAA
     1321 TTCTCCTTGG AATTTGCCCT TTTTGAGTTT GGATCTTGGT TCATTCTCAA GCCTCAGACA
     1381 GTGGTTCAAA GTTTTTTTCT TCCATTTCAG GTGTCGTGAG GTACCGGATC CTCTAGAGTC
     1441 GACTCCGGAA TAACTTCGTA TAGGATACTT TATACGAAGT TATGCAGAAT GGTAGCTGGA
     1501 TTGTAGCTGC TATTAGCAAT ATGAAACCTC TTAATAACTT CGTATAGCAT ACATTATACG
     1561 AAGTTATGGC GCGCCCTAAT ACAGACGCTG GGGCTTGCCC ATGGTGCTCT TGATGTACAG
     1621 AGCCCGCACG TTTTGCCAGT TTTTCTTAAG CAAGGACACC AAGAAATTGA CAGCCAGATG
     1681 AATGTTGTAG ACTAGCTCAT CATCGGTCAT CTTCACGTGG CCAACAGCGA CGGCCAAACA
     1741 CAGCACCTTC TTCATCTGGA ACTTGATTGT CGATTTCACC TCATCCACTT TGGCCACCAT
     1801 GTTTTCATTG TGTGTCAGCA GGGAGGGGAA CTTGCCAGCC TTGTTTAGGC CTGGGCCCAG
     1861 GATACGTGGG ATCTGCTTAA TCAGAGACTC AGAGGCCAAA AAGGCATCGT ACTTCTTAGC
     1921 CAGCTTCTTG ACCAACTTCT TGTTTTTGTT AAGCTTCTTG AGCGCCTCGA TGTCCATGTG
     1981 GGGGATATCC ACGGCCTTGG CTTCATCACA GTGCTGCTGG TCCCCCAGAA CGCACACCGA
     2041 GAACTTGGGG CGTGGGGTGG ACTTGAGCCT GACGGTGCCC GAGAAACGTT TGTCCTTCTG
     2101 AGGGTCGTAG TTCTTCAGGC TGATCTGCAG CTCCACCGTC TCCAGAAACT TGCGGCGCTT
     2161 GCGCTGGTTC CCGTGCAGGA CTTCCCGCAC CGCCTCGTAC AGGGTGTCGC GTGAGACTTT
     2221 GCTGCTGAAT TCGAAGCTTG AGCTCGAGAT CTGAGTCCGG CCGGACTTGT ACAGCTCGTC
     2281 CATGCCGAGA GTGATCCCGG CGGCGGTCAC GAACTCCAGC AGGACCATGT GATCGCGCTT
     2341 CTCGTTGGGG TCTTTGCTCA GGGCGGACTG GGTGCTCAGG TAGTGGTTGT CGGGCAGCAG
     2401 CACGGGGCCG TCGCCGATGG GGGTGTTCTG CTGGTAGTGG TCGGCGAGCT GCACGCTGCC
     2461 GTCCTCGATG TTGTGGCGGA TCTTGAAGTT CACCTTGATG CCGTTCTTCT GCTTGTCGGC
     2521 CATGATATAG ACGTTGTGGC TGTTGTAGTT GTACTCCAGC TTGTGCCCCA GGATGTTGCC
     2581 GTCCTCCTTG AAGTCGATGC CCTTCAGCTC GATGCGGTTC ACCAGGGTGT CGCCCTCGAA
     2641 CTTCACCTCG GCGCGGGTCT TGTAGTTGCC GTCGTCCTTG AAGAAGATGG TGCGCTCCTG
     2701 GACGTAGCCT TCGGGCATGG CGGACTTGAA GAAGTCGTGC TGCTTCATGT GGTCGGGGTA
     2761 GCGGCTGAAG CACTGCACGC CGTAGGTCAG GGTGGTCACG AGGGTGGGCC AGGGCACGGG
     2821 CAGCTTGCCG GTGGTGCAGA TGAACTTCAG GGTCAGCTTG CCGTAGGTGG CATCGCCCTC
     2881 GCCCTCGCCG GACACGCTGA ACTTGTGGCC GTTTACGTCG CCGTCCAGCT CGACCAGGAT
     2941 GGGCACCACC CCGGTGAACA GCTCCTCGCC CTTGCTCACC ATGGTGGCTA GCATAACTTC
     3001 GTATAAAGTA TCCTATACGA AGTTATTTGC CTTAACCCAG AAATTATCAC TGTTATTCTT
     3061 TAGAATGGTG CAAAGAATAA CTTCGTATAA TGTATGCTAT ACGAAGTTAT GAATTCGATA
     3121 TCAAGCTTAT CGATAATCAA CCTCTGGATT ACAAAATTTG TGAAAGATTG ACTGGTATTC
     3181 TTAACTATGT TGCTCCTTTT ACGCTATGTG GATACGCTGC TTTAATGCCT TTGTATCATG
     3241 CTATTGCTTC CCGTATGGCT TTCATTTTCT CCTCCTTGTA TAAATCCTGG TTGCTGTCTC
     3301 TTTATGAGGA GTTGTGGCCC GTTGTCAGGC AACGTGGCGT GGTGTGCACT GTGTTTGCTG
     3361 ACGCAACCCC CACTGGTTGG GGCATTGCCA CCACCTGTCA GCTCCTTTCC GGGACTTTCG
     3421 CTTTCCCCCT CCCTATTGCC ACGGCGGAAC TCATCGCCGC CTGCCTTGCC CGCTGCTGGA
     3481 CAGGGGCTCG GCTGTTGGGC ACTGACAATT CCGTGGTGTT GTCGGGGAAA TCATCGTCCT
     3541 TTCCTTGGCT GCTCGCCTAT GTTGCCACCT GGATTCTGCG CGGGACGTCC TTCTGCTACG
     3601 TCCCTTCGGC CCTCAATCCA GCGGACCTTC CTTCCCGCGG CCTGCTGCCG GCTCTGCGGC
     3661 CTCTTCCGCG TCTTCGCCTT CGCCCTCAGA CGAGTCGGAT CTCCCTTTGG GCCGCCTCCC
     3721 CGCATCGATA CCGAGCGCTG CTCGAGAGAT CTACGGGTGG CATCCCTGTG ACCCCTCCCC
     3781 AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA
     3841 ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG
     3901 GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG
     3961 GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT
     4021 TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG
     4081 GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT
     4141 CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA
     4201 GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTG TAGGTAACCA CGTGCGGACC
     4261 GAGCGGCCGC AGGAACCCCT AGTGATGGAG TTGGCCACTC CCTCTCTGCG CGCTCGCTCG
     4321 CTCACTGAGG CCGGGCGACC AAAGGTCGCC CGACGCCCGG GCTTTGCCCG GGCGGCCTCA
     4381 GTGAGCGAGC GAGCGCGCAG CTGCCTGCAG GGGCGCCTGA TGCGGTATTT TCTCCTTACG
     4441 CATCTGTGCG GTATTTCACA CCGCATACGT CAAAGCAACC ATAGTACGCG CCCTGTAGCG
     4501 GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG
     4561 CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC
     4621 CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC
     4681 TCGACCCCAA AAAACTTGAT TTGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA
     4741 CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA
     4801 CTGGAACAAC ACTCAACCCT ATCTCGGGCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA
     4861 TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTTAACA
     4921 AAATATTAAC GTTTACAATT TTATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT
     4981 AGTTAAGCCA GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC
     5041 TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT
     5101 TTTCACCGTC ATCACCGAAA CGCGCGAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT
     5161 AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG
     5221 TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA
     5281 GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC
     5341 ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC
     5401 CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA
     5461 TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC
     5521 CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG
     5581 GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC
     5641 CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA
     5701 TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG
     5761 AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC
     5821 CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG
     5881 CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT
     5941 TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG
     6001 CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG
     6061 CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC
     6121 AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC
     6181 ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT
     6241 TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT
     6301 AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT
     6361 GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG
     6421 CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA
     6481 GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA
     6541 AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG
     6601 CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG
     6661 CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT
     6721 ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA
     6781 GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC
     6841 TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG
     6901 AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG
     6961 CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGT
//
