LOCUS       pET28b                  5368 bp    DNA     circular UNA 06-AUG-2014
DEFINITION  .
ACCESSION   urn.local...1g-3js4sas
VERSION     urn.local...1g-3js4sas
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     CDS             2497..3579
                     /created_by="User"
                     /label=lacI
     unsure          3056..3061
                     /created_by="User"
                     /modified_by="User"
                     /label=ApaI
     unsure          3297..3302
                     /created_by="User"
                     /label=EcoR_V
     rep_origin      complement(4996..5615)
                     /created_by="User"
                     /modified_by="User"
                     /label=pBR322
     ORF             5721..6536
                     /created_by="User"
                     /label=KAN
     rep_origin      complement(6630..7085)
                     /created_by="User"
                     /label=f1 origin
     CDS             complement(158..2026)
                     /dnas_title="ORF frame 1"
                     /label=ORF frame 1
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKL
                     EEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNG
                     KLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWP
                     LIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAF
                     NKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKEL
                     AKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNI
                     PQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGIEGRGENLY
                     FQGHHHHHHHGESLFKGPRDYNPISSTICHLTNESDGHTTSLYGIGFGPFIITNKHLF
                     RRNNGTLLVQSLHGVFKVKNTTTLQQHLIDGRDMIIIRMPKDFPPFPQKLKFREPQRE
                     ERICLVTTNFQTKSMSSMVSDTSCTFPSSDGIFWKHWIQTKDGQCGSPLVSTRDGFIV
                     GIHSASNFTNTNNYFTSVPKNFMELLTNQEAQQWVSGWRLNADSVLWGGHKVFMVKPE
                     EPFQPVKEATQLMN"
     misc_feature    complement(929..952)
                     /dnas_title="MBP_F_primer"
                     /label=MBP_F_primer
     misc_feature    complement(872..889)
                     /dnas_title="6xHis"
                     /label=6xHis
     misc_feature    complement(158..886)
                     /dnas_title="6xHis"
                     /label=6xHis
     misc_feature    complement(890..913)
                     /note="TEVcutsite"
     misc_feature    158..909
                     /note="msp421"
     misc_feature    complement(1281..1311)
                     /note="MSP215"
     misc_feature    complement(138..157)
                     /note="MSP422"
     misc_feature    138..157
                     /note="MSP423"
     gene            complement(914..2026)
                     /note="MBP"
     PCR_primer      complement(2002..2051)
                     /note="MSP425"
     PCR_primer      2027..2065
                     /note="MSP424"
     misc_feature    881..932
                     /note=""
     misc_feature    complement(158..888)
                     /note="MSP445"
     misc_feature    139..157
                     /note="MSP448"
     misc_feature    complement(118..147)
                     /note="MSP449"
     source          1..7095
                     /dnas_title="MSP969"
ORIGIN      
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
      121 tgttagcagc cggatctcag tggtggtggt ggtggtgtta attcatgagt tgagtcgctt
      181 ccttaactgg ctgaaaaggc tcttcaggtt tcaccatgaa aactttatgg cccccccaca
      241 atactgagtc agcatttaat cgccaaccac taacccactg ctgcgcctcc tgatttgtca
      301 acaattccat gaagtttttc ggcacgcttg tgaaataCAt gtttgtgttg gtgaaattcg
      361 Ctgctgagtg tataccaaca atgaacccat ctctagttga tactaatgga Ttgccacact
      421 gcccaGGctt ggAttgaatc caatgcttcc agaatatgcc atcagatgaa gggaatgtgc
      481 aactagtgtc tgacaccatg ctagacatgc tcttagtttg gaagttggtt gtcacaagac
      541 atatgcgctc ttccctttgt ggctctctaa atttcagctt ttgaggaaat ggtgggaaat
      601 ccttaggcat gcgaataatt atcatgtccc tcccatcaat gaggtgttgt tgcaaagtcg
      661 tggtgttctt gaccttgaat acaccatgta gtgattggac caacagtgtt ccattatttc
      721 ttctaaacaa gtgcttgttt gtaatgatga agggaccaaa tccaatacca tacaacgatg
      781 ttgtgtgccc atcagattca ttcgtcaaat gacaaatggt gctcgatatc gggttgtaat
      841 cacgcggccc cttaaacaag ctttctccat gatgatgatg atgatgatgc gaTTGAAAAT
      901 AAAGATTTTC TCCCGAGCTC GAATTAGTCT GCGCGTCTTT CAGGGCTTCA TCGACAGTCT
      961 GACGACCGCT GGCGGCGTTG ATCACCGCAG TACGCACGGC ATACCAGAAA GCGGACATCT
     1021 GCGGGATGTT CGGCATGATT TCACCTTTCT GGGCGTTTTC CATGGTGGCG GCAATACGTG
     1081 GATCTTTCGC CAACTCTTCC TCGTAAGACT TCAGCGCTAC GGCACCCAGC GGTTTGTCTT
     1141 TATTAACCGC TTCCAGACCT TCATCAGTCA GCAGATAGTT TTCGAGGAAC TCTTTTGCCA
     1201 GCTCTTTGTT CGGACTGGCG GCGTTAATAC CTGCGCTCAG CACGCCAACG AACGGTTTGG
     1261 ATGGTTGACC CTTGAAGGTC GGCAGTACCG TTACACCATA ATTCACTTTG CTGGTGTCGA
     1321 TGTTGGACCA TGCCCACGGG CCGTTGATGG TCATCGCTGT TTCGCCTTTA TTAAAGGCAG
     1381 CTTCTGCGAT GGAGTAATCG GTGTCTGCAT TCATGTGTTT GTTTTTAATC AGGTCAACCA
     1441 GGAAGGTCAG ACCCGCTTTC GCGCCAGCGT TATCCACGCC CACGTCTTTA ATGTCGTACT
     1501 TGCCGTTTTC ATACTTGAAC GCATAACCCC CGTCAGCAGC AATCAGCGGC CAGGTGAAGT
     1561 ACGGTTCTTG CAGGTTGAAC ATCAGCGCGC TCTTACCTTT CGCTTTCAGT TCTTTATCCA
     1621 GCGCCGGGAT CTCTTCCCAG GTTTTTGGCG GGTTCGGCAG CAGATCTTTG TTATAAATCA
     1681 GCGATAACGC TTCAACAGCG ATCGGGTAAG CAATCAGCTT GCCGTTGTAA CGTACGGCAT
     1741 CCCAGGTAAA CGGATACAGC TTGTCCTGGA ACGCTTTGTC CGGGGTGATT TCAGCCAACA
     1801 GGCCAGATTG AGCGTAGCCA CCAAAGCGGT CGTGTGCCCA GAAGATAATG TCAGGGCCAT
     1861 CGCCAGTTGC CGCAACCTGT GGGAATTTCT CTTCCAGTTT ATCCGGATGC TCAACGGTGA
     1921 CTTTAATTCC GGTATCTTTC TCGAATTTCT TACCGACTTC AGCGAGACCG TTATAGCCTT
     1981 TATCGCCGTT AATCCAGATT ACCAGTTTAC CTTCTTCGAT TTTCATgtat atctccttct
     2041 taaagttaaa caaaattatt tctagagggg aattgttatc cgctcacaat tcccctatag
     2101 tgagtcgtat taatttcgcg ggatcgagat ctcgatcctc tacgccggac gcatcgtggc
     2161 cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg
     2221 ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc
     2281 aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc
     2341 ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa
     2401 gggagagcgt cgagatcccg gacaccatcg aatggcgcaa aacctttcgc ggtatggcat
     2461 gatagcgccc ggaagagagt caattcaggg tggtgaatgt gaaaccagta acgttatacg
     2521 atgtcgcaga gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca
     2581 gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag ctgaattaca
     2641 ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc gttgctgatt ggcgttgcca
     2701 cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg
     2761 atcaactggg tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta
     2821 aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc
     2881 tggatgacca ggatgccatt gctgtggaag ctgcctgcac taatgttccg gcgttatttc
     2941 ttgatgtctc tgaccagaca cccatcaaca gtattatttt ctcccatgaa gacggtacgc
     3001 gactgggcgt ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc
     3061 cattaagttc tgtctcggcg cgtctgcgtc tggctggctg gcataaatat ctcactcgca
     3121 atcaaattca gccgatagcg gaacgggaag gcgactggag tgccatgtcc ggttttcaac
     3181 aaaccatgca aatgctgaat gagggcatcg ttcccactgc gatgctggtt gccaacgatc
     3241 agatggcgct gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata
     3301 tctcggtagt gggatacgac gataccgaag acagctcatg ttatatcccg ccgttaacca
     3361 ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct
     3421 ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa
     3481 ccaccctggc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     3541 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgta
     3601 agttagctca ctcattaggc accgggatct cgaccgatgc ccttgagagc cttcaaccca
     3661 gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc
     3721 tttatcatgc aactcgtagg acaggtgccg gcagcgctct gggtcatttt cggcgaggac
     3781 cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg aatcttgcac
     3841 gccctcgctc aagccttcgt cactggtccc gccaccaaac gtttcggcga gaagcaggcc
     3901 attatcgccg gcatggcggc cccacgggtg cgcatgatcg tgctcctgtc gttgaggacc
     3961 cggctaggct ggcggggttg ccttactggt tagcagaatg aatcaccgat acgcgagcga
     4021 acgtgaagcg actgctgctg caaaacgtct gcgacctgag caacaacatg aatggtcttc
     4081 ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag cgccctgcac cattatgttc
     4141 cggatctgca tcgcaggatg ctgctggcta ccctgtggaa cacctacatc tgtattaacg
     4201 aagcgctggc attgaccctg agtgattttt ctctggtccc gccgcatcca taccgccagt
     4261 tgtttaccct cacaacgttc cagtaaccgg gcatgttcat catcagtaac ccgtatcgtg
     4321 agcatcctct ctcgtttcat cggtatcatt acccccatga acagaaatcc cccttacacg
     4381 gaggcatcag tgaccaaaca ggaaaaaacc gcccttaaca tggcccgctt tatcagaagc
     4441 cagacattaa cgcttctgga gaaactcaac gagctggacg cggatgaaca ggcagacatc
     4501 tgtgaatcgc ttcacgacca cgctgatgag ctttaccgca gctgcctcgc gcgtttcggt
     4561 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     4621 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     4681 ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg
     4741 catcagagca gattgtactg agagtgcacc atatatgcgg tgtgaaatac cgcacagatg
     4801 cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg
     4861 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     4921 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     4981 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     5041 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     5101 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     5161 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     5221 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     5281 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     5341 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     5401 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     5461 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     5521 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     5581 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     5641 gaacgaaaac tcacgttaag ggattttggt catgaacaat aaaactgtct gcttacataa
     5701 acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcttgc tctaggccgc
     5761 gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg
     5821 ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca gagttgtttc
     5881 tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact
     5941 ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg
     6001 catggttact caccactgcg atccccggga aaacagcatt ccaggtatta gaagaatatc
     6061 ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga
     6121 ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat
     6181 cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc
     6241 ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg
     6301 tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt
     6361 gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga
     6421 actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg
     6481 ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaagaat
     6541 taattcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     6601 cgcacatttc cccgaaaagt gccacctgaa attgtaaacg ttaatatttt gttaaaattc
     6661 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     6721 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     6781 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     6841 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     6901 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     6961 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     7021 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     7081 gcgtcccatt cgcca
//

