LOCUS       pET28b                  5368 bp    DNA     circular UNA 06-AUG-2014
DEFINITION  .
ACCESSION   urn.local...1g-3js4sas
VERSION     urn.local...1g-3js4sas
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     CDS             2407..3489
                     /created_by="User"
                     /label=lacI
     unsure          2966..2971
                     /created_by="User"
                     /modified_by="User"
                     /label=ApaI
     unsure          3207..3212
                     /created_by="User"
                     /label=EcoR_V
     rep_origin      complement(4906..5525)
                     /created_by="User"
                     /modified_by="User"
                     /label=pBR322
     ORF             5631..6446
                     /created_by="User"
                     /label=KAN
     rep_origin      complement(6540..6995)
                     /created_by="User"
                     /label=f1 origin
     CDS             complement(158..1936)
                     /dnas_title="ORF frame 1"
                     /label=ORF frame 1
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKL
                     EEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNG
                     KLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWP
                     LIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAF
                     NKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKEL
                     AKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNI
                     PQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGIEGRGENLY
                     FQGHHHHHHHGESLFKGPRDYNPISSTICHLTNESDGHTTSLYGIGFGPFIITNKHLF
                     RRNNGTLLVQSLHGVFKVKNTTTLQQHLIDGRDMIIIRMPKDFPPFPQKLKFREPQRE
                     ERICLVTTNFQTKSMSSMVSDTSCTFPSSDGIFWKHWIQTKDGQCGSPLVSTRDGFIV
                     GIHSASNFTNTNNYFTSVPKNFMELLTNQEAQQWVSGWRLNADSVLWGGHKVFMVKPE
                     EPFQPVKEATQLMN"
     misc_feature    complement(839..862)
                     /dnas_title="MBP_F_primer"
                     /label=MBP_F_primer
     misc_feature    complement(782..799)
                     /dnas_title="6xHis"
                     /label=6xHis
     misc_feature    complement(800..823)
                     /note="TEVcutsite"
     misc_feature    complement(1191..1221)
                     /note="MSP215"
     misc_feature    complement(138..157)
                     /note="MSP422"
     gene            complement(824..1936)
                     /note="MBP"
     PCR_primer      complement(1912..1961)
                     /note="MSP425"
     PCR_primer      1937..1975
                     /note="MSP424"
     gene            complement(158..778)
                     /note="GST"
     PCR_primer      138..180
                     /note="MSP426"
     PCR_primer      complement(758..778)
                     /note="MSP427"
     PCR_primer      761..819
                     /note="MSP428"
     misc_feature    791..842
                     /note="MSP447"
     misc_feature    complement(770..798)
                     /note="MSP450"
     source          1..7005
                     /dnas_title="pET28b MBP(no 10xN)-GENLYFEG-6his-GST"
ORIGIN      
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
      121 tgttagcagc cggatctcag tggtggtggt ggtggtgCTG CTTGCCGTTT CCGTTAATCG
      181 GGCGGTTTAC GTGTTCAGGT GACGCTAAGA AAGCTTTGAT CTTCGGACGG GCGCTCAGGC
      241 GCGCTACATA TGCGCTCAGT AACGGGAAAG CGTCTAAACA GCTAGGATTT AAAACCTGGT
      301 GAATGCGTAA TAAGTCTAAT AAGTTATAAT CAGCGAAGCT AATTTGGCTA CCAACTACGA
      361 ACGCCTGACC TCCCTGATTC TGGCTCAGTA ATGTTTCAAA CGGCTTCAGG TGTTCAGGTA
      421 ATTCTTTCAC GTACTTCTCT TTACCGGCTT CATAATTAGT GTAAATTAAC GTCGCGTATT
      481 TACAACGTAA GTCTTCCACG CCATCATTCA CCATATCCAC TAAGGCTGCT TCTTTCTGAT
      541 CTTTACCGTA TAAACCAAAT GAGCGACCTA AATGACGTAA GATCGCGTTA GACTGGTATA
      601 AGGTTAAATC ACCATCCTGA AACTTCGGTA ATTGACGGAA TAAACAGCTA GGCTTTAACG
      661 GCGGCCAGGT TTCCATTGTC ACTACTTCTT CCTTCCATGA TTGATCCTGG TCCGCTAATA
      721 ACATACGCAT CGCTTCACAA CGACCACGTA CCGGAAAGTA TGTAATGGTG TACGGCGGat
      781 gatgatgatg atgatgatga ctTTGAAAAT AAAGATTaTg TCCCGAGCTC GAATTAGTCT
      841 GCGCGTCTTT CAGGGCTTCA TCGACAGTCT GACGACCGCT GGCGGCGTTG ATCACCGCAG
      901 TACGCACGGC ATACCAGAAA GCGGACATCT GCGGGATGTT CGGCATGATT TCACCTTTCT
      961 GGGCGTTTTC CATGGTGGCG GCAATACGTG GATCTTTCGC CAACTCTTCC TCGTAAGACT
     1021 TCAGCGCTAC GGCACCCAGC GGTTTGTCTT TATTAACCGC TTCCAGACCT TCATCAGTCA
     1081 GCAGATAGTT TTCGAGGAAC TCTTTTGCCA GCTCTTTGTT CGGACTGGCG GCGTTAATAC
     1141 CTGCGCTCAG CACGCCAACG AACGGTTTGG ATGGTTGACC CTTGAAGGTC GGCAGTACCG
     1201 TTACACCATA ATTCACTTTG CTGGTGTCGA TGTTGGACCA TGCCCACGGG CCGTTGATGG
     1261 TCATCGCTGT TTCGCCTTTA TTAAAGGCAG CTTCTGCGAT GGAGTAATCG GTGTCTGCAT
     1321 TCATGTGTTT GTTTTTAATC AGGTCAACCA GGAAGGTCAG ACCCGCTTTC GCGCCAGCGT
     1381 TATCCACGCC CACGTCTTTA ATGTCGTACT TGCCGTTTTC ATACTTGAAC GCATAACCCC
     1441 CGTCAGCAGC AATCAGCGGC CAGGTGAAGT ACGGTTCTTG CAGGTTGAAC ATCAGCGCGC
     1501 TCTTACCTTT CGCTTTCAGT TCTTTATCCA GCGCCGGGAT CTCTTCCCAG GTTTTTGGCG
     1561 GGTTCGGCAG CAGATCTTTG TTATAAATCA GCGATAACGC TTCAACAGCG ATCGGGTAAG
     1621 CAATCAGCTT GCCGTTGTAA CGTACGGCAT CCCAGGTAAA CGGATACAGC TTGTCCTGGA
     1681 ACGCTTTGTC CGGGGTGATT TCAGCCAACA GGCCAGATTG AGCGTAGCCA CCAAAGCGGT
     1741 CGTGTGCCCA GAAGATAATG TCAGGGCCAT CGCCAGTTGC CGCAACCTGT GGGAATTTCT
     1801 CTTCCAGTTT ATCCGGATGC TCAACGGTGA CTTTAATTCC GGTATCTTTC TCGAATTTCT
     1861 TACCGACTTC AGCGAGACCG TTATAGCCTT TATCGCCGTT AATCCAGATT ACCAGTTTAC
     1921 CTTCTTCGAT TTTCATgtat atctccttct taaagttaaa caaaattatt tctagagggg
     1981 aattgttatc cgctcacaat tcccctatag tgagtcgtat taatttcgcg ggatcgagat
     2041 ctcgatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc
     2101 tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat
     2161 gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc
     2221 catctccttg catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact
     2281 gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgagatcccg gacaccatcg
     2341 aatggcgcaa aacctttcgc ggtatggcat gatagcgccc ggaagagagt caattcaggg
     2401 tggtgaatgt gaaaccagta acgttatacg atgtcgcaga gtatgccggt gtctcttatc
     2461 agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag
     2521 tggaagcggc gatggcggag ctgaattaca ttcccaaccg cgtggcacaa caactggcgg
     2581 gcaaacagtc gttgctgatt ggcgttgcca cctccagtct ggccctgcac gcgccgtcgc
     2641 aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg gtggtgtcga
     2701 tggtagaacg aagcggcgtc gaagcctgta aagcggcggt gcacaatctt ctcgcgcaac
     2761 gcgtcagtgg gctgatcatt aactatccgc tggatgacca ggatgccatt gctgtggaag
     2821 ctgcctgcac taatgttccg gcgttatttc ttgatgtctc tgaccagaca cccatcaaca
     2881 gtattatttt ctcccatgaa gacggtacgc gactgggcgt ggagcatctg gtcgcattgg
     2941 gtcaccagca aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg cgtctgcgtc
     3001 tggctggctg gcataaatat ctcactcgca atcaaattca gccgatagcg gaacgggaag
     3061 gcgactggag tgccatgtcc ggttttcaac aaaccatgca aatgctgaat gagggcatcg
     3121 ttcccactgc gatgctggtt gccaacgatc agatggcgct gggcgcaatg cgcgccatta
     3181 ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt gggatacgac gataccgaag
     3241 acagctcatg ttatatcccg ccgttaacca ccatcaaaca ggattttcgc ctgctggggc
     3301 aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc
     3361 tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg caaaccgcct
     3421 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     3481 gcgggcagtg agcgcaacgc aattaatgta agttagctca ctcattaggc accgggatct
     3541 cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg
     3601 actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg
     3661 gcagcgctct gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc
     3721 ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggtccc
     3781 gccaccaaac gtttcggcga gaagcaggcc attatcgccg gcatggcggc cccacgggtg
     3841 cgcatgatcg tgctcctgtc gttgaggacc cggctaggct ggcggggttg ccttactggt
     3901 tagcagaatg aatcaccgat acgcgagcga acgtgaagcg actgctgctg caaaacgtct
     3961 gcgacctgag caacaacatg aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg
     4021 cggaagtcag cgccctgcac cattatgttc cggatctgca tcgcaggatg ctgctggcta
     4081 ccctgtggaa cacctacatc tgtattaacg aagcgctggc attgaccctg agtgattttt
     4141 ctctggtccc gccgcatcca taccgccagt tgtttaccct cacaacgttc cagtaaccgg
     4201 gcatgttcat catcagtaac ccgtatcgtg agcatcctct ctcgtttcat cggtatcatt
     4261 acccccatga acagaaatcc cccttacacg gaggcatcag tgaccaaaca ggaaaaaacc
     4321 gcccttaaca tggcccgctt tatcagaagc cagacattaa cgcttctgga gaaactcaac
     4381 gagctggacg cggatgaaca ggcagacatc tgtgaatcgc ttcacgacca cgctgatgag
     4441 ctttaccgca gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     4501 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     4561 ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat
     4621 agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     4681 atatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc
     4741 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     4801 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     4861 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     4921 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     4981 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     5041 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     5101 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     5161 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     5221 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     5281 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     5341 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     5401 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     5461 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     5521 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     5581 catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata
     5641 ttcaacggga aacgtcttgc tctaggccgc gattaaattc caacatggat gctgatttat
     5701 atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt
     5761 atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg
     5821 atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca
     5881 tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg atccccggga
     5941 aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc
     6001 tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg
     6061 atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga
     6121 gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata
     6181 aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc
     6241 ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag
     6301 accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac
     6361 agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc
     6421 atttgatgct cgatgagttt ttctaagaat taattcatga gcggatacat atttgaatgt
     6481 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgaa
     6541 attgtaaacg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
     6601 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
     6661 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
     6721 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
     6781 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     6841 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
     6901 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
     6961 cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgcca
//

