LOCUS       3316_mTQ2_Utroph        5516 bp ds-DNA     circular     07-JUN-2017
DEFINITION  Mammalian expression vector.
SOURCE      
  ORGANISM  
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     promoter        1..589
                     /vntifkey="29"
                     /note="CMV promoter"
                     /label=CMV\promoter
                     /ApEinfo_fwdcolor="seashell4"
                     /ApEinfo_revcolor="seashell4"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_marker     3412..4206
                     /vntifkey="22"
                     /note="Kanamycin / Neomycin resistance"
                     /label=Kan\/\Neo\resistance
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     polyA_signal    2335..2369
                     /vntifkey="25"
                     /note="Simian Virus 40 polyadenylation signal"
                     /label=SV40\polyA
                     /ApEinfo_fwdcolor="seashell4"
                     /ApEinfo_revcolor="seashell4"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      2432..2887
                     /vntifkey="33"
                     /note="f1 Origin of Replication (packages the noncoding
                     strand of EGFP"
                     /label=f1\ORI
                     /ApEinfo_fwdcolor="SlateGray3"
                     /ApEinfo_revcolor="SlateGray3"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2949..2977
                     /vntifkey="21"
                     /note="Bacterial promoter for expression Kan R gene"
                     /label=Bact.\promoter
                     /ApEinfo_fwdcolor="maroon4"
                     /ApEinfo_revcolor="maroon4"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3228..3363
                     /vntifkey="21"
                     /note="Simian Virus 40 Origin of Replication"
                     /label=SV40\ORI
                     /ApEinfo_fwdcolor="maroon4"
                     /ApEinfo_revcolor="maroon4"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3052..3290
                     /vntifkey="21"
                     /note="Simian Virus 40 early promoter"
                     /label=SV40\early\promoter
                     /ApEinfo_fwdcolor="maroon4"
                     /ApEinfo_revcolor="maroon4"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     polyA_signal    4442..4460
                     /vntifkey="25"
                     /note="Herpes simplex virus thymidine kinase
                     polyadenylation signal"
                     /label=HSV\TK\polyA
                     /ApEinfo_fwdcolor="seashell4"
                     /ApEinfo_revcolor="seashell4"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      4791..5434
                     /vntifkey="33"
                     /note="ColE1 Origin Of Replication"
                     /label=ColE1\ORI
                     /ApEinfo_fwdcolor="SlateGray3"
                     /ApEinfo_revcolor="SlateGray3"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    613..1327
                     /note="mTq2"
                     /label=mTurquoise2
                     /ApEinfo_fwdcolor="maroon4"
                     /ApEinfo_revcolor="maroon4"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1328..2127
                     /label=UtrCH
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="#ffff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgctA
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgtcc tggggcgtgc agtgcttcgc ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac TtTagcgaca acgtctatat caccgccgac
     1081 aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcggc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc aagctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaAGT CCGGAACCAT GGCCAAGTAT GGAGAACATG AAGCCAGTCC TGACAATGGG
     1381 CAGAACGAAT TCAGTGATAT CATTAAGTCC AGATCTGATG AACACAATGA CGTACAGAAG
     1441 AAAACCTTTA CCAAATGGAT AAATGCTCGA TTTTCAAAGA GTGGGAAACC ACCCATCAAT
     1501 GATATGTTCA CAGACCTCAA AGATGGAAGG AAGCTATTGG ATCTTCTAGA AGGCCTCACA
     1561 GGAACATCAC TGCCAAAGGA ACGTGGTTCC ACAAGGGTAC ATGCCTTAAA TAACGTCAAC
     1621 AGAGTGCTGC AGGTTTTACA TCAGAACAAT GTGGAATTAG TGAATATAGG GGGAACCGAC
     1681 ATTGTGGATG GAAATCACAA ACTGACTTTG GGGTTACTTT GGAGCATCAT TTTGCACTGG
     1741 CAGGTGAAAG ATGTCATGAA GGATGTCATG TCGGACCTGC AGCAGACGAA CAGTGAGAAG
     1801 ATCCTGCTCA GCTGGGTGCG TCAGACCACC AGGCCCTACA GCCAAGTCAA CGTCCTCAAC
     1861 TTCACCACCA GCTGGACAGA TGGACTCGCC TTTAATGCTG TCCTCCACCG ACATAAACCT
     1921 GATCTCTTCA GCTGGGATAA AGTTGTCAAA ATGTCACCAA TTGAGAGACT TGAACATGCC
     1981 TTCAGCAAGG CTCAAACTTA TTTGGGAATT GAAAAGCTGT TAGATCCTGA AGATGTTGCC
     2041 GTTCGGCTTC CTGACAAGAA ATCCATAATT ATGTATTTAA CATCTTTGTT TGAGGTGCTA
     2101 CCTCAGCAAG TCACCATAGA CTAACTCGAG CTgctcaagc ttcgaattct gcagtcgacg
     2161 gtaccgcggg cccgggatcc accggatcta gataactgat cataatcagc cataccacat
     2221 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     2281 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     2341 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     2401 tgtccaaact catcaatgta tcttaacgcg taaattgtaa gcgttaatat tttgttaaaa
     2461 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     2521 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     2581 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     2641 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     2701 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     2761 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     2821 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     2881 ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     2941 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     3001 tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag
     3061 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     3121 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     3181 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     3241 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     3301 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     3361 gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg catgattgaa
     3421 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     3481 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     3541 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag
     3601 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     3661 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     3721 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     3781 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     3841 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     3901 gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat
     3961 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     4021 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     4081 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     4141 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     4201 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     4261 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     4321 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta
     4381 gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg
     4441 caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc
     4501 ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc
     4561 gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag
     4621 ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata ctttagattg
     4681 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     4741 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     4801 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     4861 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     4921 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     4981 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     5041 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     5101 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     5161 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     5221 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     5281 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     5341 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     5401 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     5461 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc atgcat
//
