LOCUS       1857_EB3_mTQ2_N1        5570 bp ds-DNA     circular     07-JUN-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    1857_EB3-mTQ2-N1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Mar 1, 2016 from SnapGene Viewer 3.0.3
            http://www.snapgene.com
COMMENT     LOCUS Exported File 4733 bp ds-DNA circular SYN 07-SEP-2012
            DEFINITION Vector for fusing EGFP to the C-terminus of a partner
            protein. ACCESSION U55762 VERSION . KEYWORDS pEGFP-N1 SOURCE
            synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE
            1 (bases 1 to 4733) AUTHORS Clontech TITLE Direct Submission
            JOURNAL  Exported Oct 31, 2012 from SnapGene Viewer 1.3.3
            http://www.snapgene.com COMMENT
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     source          1..5570
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=source:synthetic DNA construct
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     source          1..4981
                     /note="synthetic DNA construct"
                     /label=synthetic DNA construct
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2..2
                     /note="MCS"
                     /note="multiple cloning site of fluorescent protein
                     plasmids"
                     /label=MCS
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    63..905
                     /note="EB3"
                     /note="EB3: dbj|AB528380.1| Synthetic construct DNA,
                     clone: pF1KB4992, Homo sapiens MAPRE3 gene for
                     microtubule-associated protein, RP/EB family, member 3,
                     without stop codon, in Flexi system"
                     /label=EB3
                     /ApEinfo_fwdcolor="#53a6a6"
                     /ApEinfo_revcolor="#53a6a6"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    927..1636
                     /note="mTq2"
                     /label=mTq2
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ffff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     polyA_signal    1769..1890
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
                     /label=SV40 poly(A) signal
                     /ApEinfo_fwdcolor="#ff3eee"
                     /ApEinfo_revcolor="#ff3eee"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     polyA_signal    1800..1834
                     /note="SV40 polyA"
                     /label=SV40 polyA
                     /ApEinfo_fwdcolor="#ff3eee"
                     /ApEinfo_revcolor="#ff3eee"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(1897..2352)
                     /direction=LEFT
                     /note="LEFT"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label=LEFT
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        2414..2442
                     /note="Bact. promoter"
                     /note="Bacterial promoter"
                     /label=Bact. promoter
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        2485..2842
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
                     /label=SV40 promoter
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      2693..2828
                     /direction=RIGHT
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
                     /label=SV40 ori
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2877..3671
                     /note="Kanamycin / Neomycin R"
                     /note="Kanamycin / Neomycin Resistance"
                     /label=Kanamycin / Neomycin R
                     /ApEinfo_fwdcolor="#dfe6e3"
                     /ApEinfo_revcolor="#dfe6e3"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3903..3950
                     /note="HSV TK poly(A) signal"
                     /note="herpesvirus thymidine kinase polyadenylation
                     signal"
                     /label=HSV TK poly(A) signal
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3907..3925
                     /note="HSV TK polyA"
                     /label=HSV TK polyA
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      4256..4899
                     /note="ColE1 ORI"
                     /note="ColE1 Origin of Replication"
                     /label=ColE1 ORI
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        4982..5570
                     /note="CMV promoter"
                     /label=CMV promoter
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 cgctagcgct accggactca gatctcgagc tcaagcttcg aattctgcag tcgactgcca
       61 ccatggccgt caatgtgtac tccacatctg tgaccagtga aaatctgagt cgccatgata
      121 tgcttgcatg ggtcaacgac tccctgcacc tcaactatac caagatagaa cagctttgtt
      181 caggggcagc ctactgccag ttcatggaca tgctcttccc cggctgtgtg cacttgagga
      241 aagtgaagtt ccaggccaaa ctagagcatg aatacatcca caacttcaag gtgctgcaag
      301 cagctttcaa gaagatgggt gttgacaaaa tcattcctgt agagaaatta gtgaaaggaa
      361 aattccaaga taattttgag tttattcagt ggtttaagaa attctttgac gcaaactatg
      421 atggaaagga ttacaaccct ctgctggcgc ggcagggcca ggacgtagcg ccacctccta
      481 acccaggtga tcagatcttc aacaaatcca agaaactcat tggcacagca gttccacaga
      541 ggacgtcccc cacaggccca aaaaacatgc agacctctgg ccggctgagc aatgtggccc
      601 ccccctgcat tctccggaag aatcctccat cagcccgaaa tggcggccat gagactgatg
      661 cccaaattct tgaactcaac caacagctgg tggacttgaa gctgacagtg gatgggctgg
      721 agaaggaacg tgacttctac ttcagcaaac ttcgtgacat cgagctcatc tgccaggagc
      781 atgaaagtga aaacagccct gttatctcag gcatcattgg catcctctat gccacagagg
      841 aaggattcgc accccctgag gacgatgaga ttgaagagca tcaacaagaa gaccaggacg
      901 agtacctgga tccaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg
      961 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     1021 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     1081 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gtcctggggc gtgcagtgct
     1141 tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     1201 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     1261 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     1321 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctactttagc gacaacgtct
     1381 atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc cgccacaaca
     1441 tcgaggacgg cggcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     1501 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccaagctg agcaaagacc
     1561 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     1621 tcggcatgga cgagctgtac aagtaaagcg gccgcgactc tagatcataa tcagccatac
     1681 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     1741 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     1801 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     1861 tggtttgtcc aaactcatca atgtatctta aggcgtaaat tgtaagcgtt aatattttgt
     1921 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
     1981 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     2041 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     2101 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
     2161 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     2221 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     2281 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     2341 tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     2401 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     2461 aataatattg aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca
     2521 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     2581 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     2641 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     2701 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     2761 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     2821 tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga
     2881 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     2941 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     3001 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag
     3061 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     3121 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     3181 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     3241 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     3301 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     3361 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg
     3421 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     3481 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     3541 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     3601 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     3661 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
     3721 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     3781 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     3841 ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat
     3901 gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg
     3961 ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata
     4021 cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct
     4081 cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta
     4141 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     4201 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     4261 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     4321 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     4381 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     4441 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     4501 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     4561 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     4621 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     4681 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     4741 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     4801 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     4861 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     4921 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca
     4981 ttagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
     5041 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     5101 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     5161 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     5221 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
     5281 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     5341 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
     5401 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
     5461 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
     5521 tacggtggga ggtctatata agcagagctg gtttagtgaa ccgtcagatc 
//
