LOCUS       _mScarletI_Lamp1        5948 bp ds-DNA     circular     07-JUN-2017
DEFINITION  Mammalian expression vector.
SOURCE      
  ORGANISM   
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     promoter        1..589
                     /dnas_title="CMV promoter"
                     /vntifkey=29
                     /note="CMV promoter"
                     /label=CMV\promoter
                     /ApEinfo_fwdcolor="seashell4"
                     /ApEinfo_revcolor="seashell4"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_marker     3844..4638
                     /dnas_title="Kan / Neo resistance"
                     /vntifkey=22
                     /note="Kanamycin / Neomycin resistance"
                     /label=Kan\/\Neo\resistance
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     polyA_signal    2767..2801
                     /dnas_title="SV40 polyA"
                     /vntifkey=25
                     /note="Simian Virus 40 polyadenylation signal"
                     /label=SV40\polyA
                     /ApEinfo_fwdcolor="seashell4"
                     /ApEinfo_revcolor="seashell4"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      2864..3319
                     /dnas_title="f1 ORI"
                     /vntifkey=33
                     /note="f1 Origin of Replication (packages the noncoding
                     strand of EGFP"
                     /label=f1\ORI
                     /ApEinfo_fwdcolor="SlateGray3"
                     /ApEinfo_revcolor="SlateGray3"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3381..3409
                     /dnas_title="Bact. promoter"
                     /vntifkey=21
                     /note="Bacterial promoter for expression Kan R gene"
                     /label=Bact.\promoter
                     /ApEinfo_fwdcolor="maroon4"
                     /ApEinfo_revcolor="maroon4"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3660..3795
                     /dnas_title="SV40 ORI"
                     /vntifkey=21
                     /note="Simian Virus 40 Origin of Replication"
                     /label=SV40\ORI
                     /ApEinfo_fwdcolor="maroon4"
                     /ApEinfo_revcolor="maroon4"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3484..3722
                     /dnas_title="SV40 early promoter"
                     /vntifkey=21
                     /note="Simian Virus 40 early promoter"
                     /label=SV40\early\promoter
                     /ApEinfo_fwdcolor="maroon4"
                     /ApEinfo_revcolor="maroon4"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     polyA_signal    4874..4892
                     /dnas_title="HSV TK polyA"
                     /vntifkey=25
                     /note="Herpes simplex virus thymidine kinase
                     polyadenylation signal"
                     /label=HSV\TK\polyA
                     /ApEinfo_fwdcolor="seashell4"
                     /ApEinfo_revcolor="seashell4"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      5223..5866
                     /dnas_title="ColE1 ORI"
                     /vntifkey=33
                     /note="ColE1 Origin Of Replication"
                     /label=ColE1\ORI
                     /ApEinfo_fwdcolor="SlateGray3"
                     /ApEinfo_revcolor="SlateGray3"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1349..2611
                     /label=Lamp1
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="#ffff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    613..1305
                     /label=mScarleti
                     /ApEinfo_fwdcolor="#ff5555"
                     /ApEinfo_revcolor="#ff5555"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gCaGTGatca aggagttcat gCGGttcaag
      661 gtgcacatgg agggctccAt gaacggccac gagttcgaga tcgagggcga gggcgagggc
      721 CGCccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc
      781 ttcTcctggg acatcctgtc ccctcagttc atgtacggct ccagggcctT caTcaagcac
      841 cccgccgaca tccccgacta cTATaagcAg tccttccccg agggcttcaA gtgggagcgc
      901 gtgatgaact tcgaggacgg cggcGCCgtg accgtgaccc aggacAcctc cctgGAGgac
      961 ggcACCCTGa tctacaaggt gaagCTCcgc ggcaccaact tcccTCCTga cggccccgta
     1021 atgcagaaga agacAatggg ctggGAAGCA tccACCgagc ggTTGtaccc cGAGgacggc
     1081 GTGctgaagg gcgacATTAA GATGGCCctg CGCctgaagg acggcggcCG CtacCTGGCG
     1141 GACTTCaaga ccacctacAA Ggccaagaag cccgtgcagA tgcccggcgc ctacaacgtc
     1201 GACCGCAagT tggacatcaC ctcccacaac gaggactaca ccGTGgtgga acagtacgaa
     1261 cgcTCCgagg gccgccactc caccggcggc atggacgagc tgtacaagtc cggactcaga
     1321 tctcgagctc aagcttcgaa ttctgcagTC GACATGGCGG CCCCCGgcag cgcccggcga
     1381 cccctgctgc tgctactgct gttgctgctg ctcggcctca tgcattgtgc gtcagcagca
     1441 atgtttatgg tgaaaaatgg caacgggacc gcgtgcataa tggccaactt ctctgctgcc
     1501 ttctcagtga actacgacac caagagtggc cctaagaaca tgacctttga cctgccatca
     1561 gatgccacag tggtgctcaa ccgcagctcc tgtggaaaag agaacacttc tgaccccagt
     1621 ctcgtgattg cttttggaag aggacataca ctcactctca atttcacgag aaatgcaaca
     1681 cgttacagcg tccagctcat gagttttgtt tataacttgt cagacacaca ccttttcccc
     1741 aatgcgagct ccaaagaaat caagactgtg gaatctataa ctgacatcag ggcagatata
     1801 gataaaaaat acagatgtgt tagtggcacc caggtccaca tgaacaacgt gaccgtaacg
     1861 ctccatgatg ccaccatcca ggcgtacctt tccaacagca gcttcagcag gggagagaca
     1921 cgctgtgaac aagacaggcc ttccccaacc acagcgcccc ctgcgccacc cagcccctcg
     1981 ccctcacccg tgcccaagag cccctctgtg gacaagtaca acgtgagcgg caccaacggg
     2041 acctgcctgc tggccagcat ggggctgcag ctgaacctca cctatgagag gaaggacaac
     2101 acgacggtga caaggcttct caacatcaac cccaacaaga cctcggccag cgggagctgc
     2161 ggcgcccacc tggtgactct ggagctgcac agcgagggca ccaccgtcct gctcttccag
     2221 ttcgggatga atgcaagttc tagccggttt ttcctacaag gaatccagtt gaatacaatt
     2281 cttcctgacg ccagagaccc tgcctttaaa gctgccaacg gctccctgcg agcgctgcag
     2341 gccacagtcg gcaattccta caagtgcaac gcggaggagc acgtccgtgt cacgaaggcg
     2401 ttttcagtca atatattcaa agtgtgggtc caggctttca aggtggaagg tggccagttt
     2461 ggctctgtgg aggagtgtct gctggacgag aacagcatgc tgatccccat cgctgtgggt
     2521 ggtgccctgg cggggctggt cctcatcgtc ctcatcgcct acctcgtcgg caggaagagg
     2581 agtcACGCAG GCTACCAGAC TATCAAGGAT Ccaccggatc tagataactg atcataatca
     2641 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     2701 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     2761 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     2821 ctagttgtgg tttgtccaaa ctcatcaatg tatcttaacg cgtaaattgt aagcgttaat
     2881 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
     2941 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
     3001 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
     3061 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
     3121 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
     3181 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
     3241 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
     3301 gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     3361 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     3421 atgcttcaat aatattgaaa aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg
     3481 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     3541 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     3601 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     3661 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     3721 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     3781 gcttttttgg aggcctaggc ttttgcaaag atcgatcaag agacaggatg aggatcgttt
     3841 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     3901 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     3961 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     4021 ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     4081 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     4141 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     4201 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     4261 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     4321 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc
     4381 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     4441 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     4501 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     4561 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     4621 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca
     4681 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     4741 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     4801 tcgcccaccc tagggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc
     4861 gcgctatgac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcata
     4921 aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg
     4981 gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc
     5041 cagggctcgc agccaacgtc ggggcggcag gccctgccat agcctcaggt tactcatata
     5101 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     5161 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     5221 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     5281 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     5341 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     5401 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     5461 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     5521 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     5581 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     5641 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     5701 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     5761 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     5821 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     5881 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     5941 ccatgcat     
//
