LOCUS       25_ITPKA_mScarle        4793 bp ds-DNA     circular     08-JUN-2017
DEFINITION  Mammalian expression vector.
SOURCE      
  ORGANISM   
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     promoter        1..589
                     /vntifkey="29"
                     /note="CMV promoter"
                     /label=CMV\promoter
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      4068..4711
                     /vntifkey="33"
                     /note="ColE1 Origin of Replication"
                     /label=ColE1\ORI
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3719..3737
                     /vntifkey="21"
                     /label=HSV\TK\polyA
                     /ApEinfo_fwdcolor=#008000
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    628..726
                     /label=ITPKA
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        2338..2567
                     /vntifkey="29"
                     /label=SV40\early\promoter
                     /ApEinfo_fwdcolor=#408080
                     /ApEinfo_revcolor=#408080
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      2505..2640
                     /vntifkey="33"
                     /note="SV40 origin of replication"
                     /label=SV40\ORI
                     /ApEinfo_fwdcolor=#808000
                     /ApEinfo_revcolor=#808000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        2226..2254
                     /vntifkey="30"
                     /note="Bacterial promoter"
                     /label=Bact.\promoter
                     /ApEinfo_fwdcolor=#000080
                     /ApEinfo_revcolor=#000080
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     polyA_signal    1612..1646
                     /vntifkey="25"
                     /label=SV40\polyA
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    760..1453
                     /label=mScarletI
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_marker     2689..3483
                     /vntifkey="22"
                     /note="Kanamycin / Neomycin Resistance"
                     /label=Kanamycin\/\Neomycin\R
                     /ApEinfo_fwdcolor=#690f96
                     /ApEinfo_revcolor=#690f96
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      4015..4697
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      complement(1841..2147)
                     /label=F1 ori
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             2692..3483
                     /label=Kan/neoR
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        2458..2654
                     /label=SV40 early promoter
                     /ApEinfo_fwdcolor=cornflower blue
                     /ApEinfo_revcolor=cornflower blue
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct cAAGCTTATG GGCATGGCGC GACCACGGGG CGCGGGGCCC
      661 TGCAGCCCCG GGTTGGAGCG GGCTCCGCGC CGGAGCGTCG GGGAGCTGCG CCTGCTCTTC
      721 GAAGCGGGCG GATCTGGATC GGATCCaccg gtcgccacca tggtgagcaa gggcgaggCa
      781 GTGatcaagg agttcatgCG Gttcaaggtg cacatggagg gctccAtgaa cggccacgag
      841 ttcgagatcg agggcgaggg cgagggcCGC ccctacgagg gcacccagac cgccaagctg
      901 aaggtgacca agggtggccc cctgcccttc Tcctgggaca tcctgtcccc tcagttcatg
      961 tacggctcca gggcctTcaT caagcacccc gccgacatcc ccgactacTA TaagcAgtcc
     1021 ttccccgagg gcttcaAgtg ggagcgcgtg atgaacttcg aggacggcgg cGCCgtgacc
     1081 gtgacccagg acAcctccct gGAGgacggc ACCCTGatct acaaggtgaa gCTCcgcggc
     1141 accaacttcc cTCCTgacgg ccccgtaatg cagaagaaga cAatgggctg gGAAGCAtcc
     1201 ACCgagcggT TGtaccccGA GgacggcGTG ctgaagggcg acATTAAGAT GGCCctgCGC
     1261 ctgaaggacg gcggcCGCta cCTGGCGGAC TTCaagacca cctacAAGgc caagaagccc
     1321 gtgcagAtgc ccggcgccta caacgtcGAC CGCAagTtgg acatcaCctc ccacaacgag
     1381 gactacaccG TGgtggaaca gtacgaacgc TCCgagggcc gccactccac cggcggcatg
     1441 gacgagctgt acaagtaaag cggccgcgac tctagatcat aatcagccat accacatttg
     1501 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     1561 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     1621 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     1681 ccaaactcat caatgtatct taaggcgtaa attgtaagcg ttaatatttt gttaaaattc
     1741 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     1801 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     1861 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     1921 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     1981 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     2041 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     2101 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     2161 gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     2221 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     2281 tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt
     2341 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     2401 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     2461 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     2521 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     2581 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     2641 taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
     2701 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
     2761 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     2821 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca
     2881 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     2941 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     3001 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     3061 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     3121 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     3181 ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc
     3241 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     3301 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     3361 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     3421 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     3481 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag
     3541 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     3601 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg
     3661 ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa
     3721 taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt
     3781 cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg
     3841 tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca
     3901 acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt tagattgatt
     3961 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     4021 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     4081 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     4141 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     4201 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     4261 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     4321 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     4381 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     4441 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     4501 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     4561 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     4621 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     4681 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     4741 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg cat
//
