LOCUS 27_mScarletI_Lam 6470 bp ds-DNA circular 07-JUN-2017 DEFINITION Mammalian expression vector. SOURCE ORGANISM COMMENT COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers promoter 1..589 /dnas_title="CMV promoter" /vntifkey=29 /note="CMV promoter" /label=CMV\promoter /ApEinfo_fwdcolor="seashell4" /ApEinfo_revcolor="seashell4" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 1373..3133 /label=LMNB1 /ApEinfo_fwdcolor="#00ffff" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_marker 4366..5160 /dnas_title="Kan / Neo resistance" /vntifkey=22 /note="Kanamycin / Neomycin resistance" /label=Kan\/\Neo\resistance /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 1324..1329 /label=mScarleti /ApEinfo_fwdcolor="#ff5555" /ApEinfo_revcolor="#ff5555" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" polyA_signal 3289..3323 /dnas_title="SV40 polyA" /vntifkey=25 /note="Simian Virus 40 polyadenylation signal" /label=SV40\polyA /ApEinfo_fwdcolor="seashell4" /ApEinfo_revcolor="seashell4" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" rep_origin 3386..3841 /dnas_title="f1 ORI" /vntifkey=33 /note="f1 Origin of Replication (packages the noncoding strand of EGFP" /label=f1\ORI /ApEinfo_fwdcolor="SlateGray3" /ApEinfo_revcolor="SlateGray3" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 3903..3931 /dnas_title="Bact. promoter" /vntifkey=21 /note="Bacterial promoter for expression Kan R gene" /label=Bact.\promoter /ApEinfo_fwdcolor="maroon4" /ApEinfo_revcolor="maroon4" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 4182..4317 /dnas_title="SV40 ORI" /vntifkey=21 /note="Simian Virus 40 Origin of Replication" /label=SV40\ORI /ApEinfo_fwdcolor="maroon4" /ApEinfo_revcolor="maroon4" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 4006..4244 /dnas_title="SV40 early promoter" /vntifkey=21 /note="Simian Virus 40 early promoter" /label=SV40\early\promoter /ApEinfo_fwdcolor="maroon4" /ApEinfo_revcolor="maroon4" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" polyA_signal 5396..5414 /dnas_title="HSV TK polyA" /vntifkey=25 /note="Herpes simplex virus thymidine kinase polyadenylation signal" /label=HSV\TK\polyA /ApEinfo_fwdcolor="seashell4" /ApEinfo_revcolor="seashell4" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" rep_origin 5745..6388 /dnas_title="ColE1 ORI" /vntifkey=33 /note="ColE1 Origin Of Replication" /label=ColE1\ORI /ApEinfo_fwdcolor="SlateGray3" /ApEinfo_revcolor="SlateGray3" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 613..1329 /label=mTq2 /ApEinfo_fwdcolor="#0080c0" /ApEinfo_revcolor="#0080c0" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 CCGGTCGCCA CCatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg 661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc 721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg 781 ccctggccca ccctcgtgac caccctgtcc tggggcgtgc agtgcttcgc ccgctacccc 841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag 961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac 1021 atcctggggc acaagctgga gtacaactac TtTagcgaca acgtctatat caccgccgac 1081 aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcggc 1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg 1201 cccgacaacc actacctgag cacccagtcc aagctgagca aagaccccaa cgagaagcgc 1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag 1321 ctgtacaaga agtccggact cagatctcga gctcaagctt cgaattctgc agATGGCGAC 1381 TGCGACCCCC GTGCCGCCGC GGATGGGCAG CCGCGCTGGC GGCCCCACCA CGCCGCTGAG 1441 CCCCACGCGC CTGTCGCGGC TCCAGGAGAA GGAGGAGCTG CGCGAGCTCA ATGACCGGCT 1501 GGCGGTGTAC ATCGACAAGG TGCGCAGCCT GGAGACGGAG AACAGCGCGC TGCAGCTGCA 1561 GGTGACGGAG CGCGAGGAGG TGCGCGGCCG TGAGCTCACC GGCCTCAAGG CGCTCTACGA 1621 GACCGAGCTG GCCGACGCGC GACGCGCGCT CGACGACACG GCCCGCGAGC GCGCCAAGCT 1681 GCAGATCGAG CTGGGCAAGT GCAAGGCGGA ACACGACCAG CTGCTCCTCA ACTATGCTAA 1741 GAAGGAATCT GATCTTAATG GCGCCCAGAT CAAGCTTCGA GAATATGAAG CAGCACTGAA 1801 TTCGAAAGAT GCAGCTCTTG CTACTGCACT TGGTGACAAA AAAAGTTTAG AGGGAGATTT 1861 GGAGGATCTG AAGGATCAGA TTGCCCAGTT GGAAGCCTCC TTAGCTGCAG CCAAAAAACA 1921 GTTAGCAGAT GAAACTTTAC TTAAAGTAGA TTTGGAGAAT CGTTGTCAGA GCCTTACTGA 1981 GGACTTGGAG TTTCGCAAAA GCATGTATGA AGAGGAGATT AACGAGACCA GAAGGAAGCA 2041 TGAAACGCGC TTGGTAGAGG TGGATTCTGG GCGTCAAATT GAGTATGAGT ACAAGCTGGC 2101 GCAAGCCCTT CATGAGATGA GAGAGCAACA TGATGCCCAA GTGAGGCTGT ATAAGGAGGA 2161 GCTGGAGCAG ACTTACCATG CCAAACTTGA GAATGCCAGA CTGTCATCAG AGATGAATAC 2221 TTCTACTGTC AACAGTGCCA GGGAAGAACT GATGGAAAGC CGCATGAGAA TTGAGAGCCT 2281 TTCATCCCAG CTTTCTAATC TACAGAAAGA GTCTAGAGCA TGTTTGGAAA GGATTCAAGA 2341 ATTAGAGGAC TTGCTTGCTA AAGAAAAAGA CAACTCTCGT CGCATGCTGA CAGACAAAGA 2401 GAGAGAGATG GCGGAAATAA GGGATCAAAT GCAGCAACAG CTGAATGACT ATGAACAGCT 2461 TCTTGATGTA AAGTTAGCCC TGGACATGGA AATCAGTGCT TACAGGAAAC TCTTAGAAGG 2521 CGAAGAAGAG AGGTTGAAGC TGTCTCCAAG CCCTTCTTCC CGTGTGACAG TATCCCGAGC 2581 ATCCTCAAGT CGTAGTGTAC GTACAACTAG AGGAAAGCGG AAGAGGGTTG ATGTGGAAGA 2641 ATCAGAGGCG AGTAGTAGTG TTAGCATCTC TCATTCCGCC TCAGCCACTG GAAATGTTTG 2701 CATCGAAGAA ATTGATGTTG ATGGGAAATT TATCCGCTTG AAGAACACTT CTGAACAGGA 2761 TCAACCAATG GGAGGCTGGG AGATGATCAG AAAAATTGGA GACACATCAG TCAGTTATAA 2821 ATATACCTCA AGATATGTGC TGAAGGCAGG CCAGACTGTT ACAATTTGGG CTGCAAACGC 2881 TGGTGTCACA GCCAGCCCCC CAACTGACCT CATCTGGAAG AACCAGAACT CGTGGGGCAC 2941 TGGCGAAGAT GTGAAGGTTA TATTGAAAAA TTCTCAGGGA GAGGAGGTTG CTCAAAGAAG 3001 TACAGTCTTT AAAACAACCA TACCTGAAGA AGAGGAGGAG GAGGAAGAAG CAGCTGGAGT 3061 GGTTGTTGAG GAAGAACTTT TCCACCAGCA GGGAACCCCA AGAGCATCCA ATAGAAGCTG 3121 TGCAATTATG TAAcaccgga tctagataac tgatcataat cagccatacc acatttgtag 3181 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga 3241 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata 3301 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca 3361 aactcatcaa tgtatcttaa cgcgtaaatt gtaagcgtta atattttgtt aaaattcgcg 3421 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct 3481 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt 3541 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat 3601 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca 3661 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac 3721 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta 3781 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg 3841 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata 3901 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga 3961 aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg 4021 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 4081 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc 4141 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc 4201 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg 4261 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag 4321 gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat 4381 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca 4441 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg 4501 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg 4561 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact 4621 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct 4681 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg 4741 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt 4801 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc 4861 gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc 4921 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga 4981 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc 5041 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt 5101 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga 5161 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt 5221 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg 5281 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga 5341 ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa 5401 aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc 5461 agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt 5521 cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg 5581 tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag attgatttaa 5641 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca 5701 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag 5761 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac 5821 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa 5881 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc 5941 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag 6001 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac 6061 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc 6121 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc 6181 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca 6241 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc 6301 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg 6361 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct 6421 ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat //