LOCUS       Exported               11113 bp ds-DNA     circular SYN 07-MAR-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    2015_11_02 pIW447 pJSK316-CRISPR-Tef1KmCas-KmRPR1p-GlytRNA-Xdh1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11113)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 21, 2017 from SnapGene 2.8.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11113
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        190..410
                     /gene="S. cerevisiae URA3"
                     /note="URA3 promoter"
     CDS             411..1214
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /note="URA3"
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      complement(1343..1798)
                     /direction=LEFT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     CDS             complement(1581..2000)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="fragment of beta-galactosidase"
                     /note="lacZ'"
                     /translation="WSSNSPYSESYYNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWR
                     NSEEARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPS
                     FLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD"
     primer_bind     1943..1959
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1966..1984
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    1993..2000
                     /note="MCS"
                     /note="pBluescript multiple cloning site"
     promoter        2001..2369
                     /gene="S. cerevisiae TEF1"
                     /note="TEF1 promoter"
                     /note="promoter for EF-1-alpha"
     misc_feature    2370..2430
                     /note="Cut sites and overlap with plasmid"
     CDS             2431..6534
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /note="KmCas9"
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPENYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    3769..3798
                     /note="OVERLAP"
     misc_feature    5197..5226
                     /note="OVERLAP"
     CDS             6547..6567
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /note="SV40 NLS"
                     /translation="PKKKRKV"
     misc_feature    6571..6613
                     /note="Cut sites and overlap with plasmid"
     terminator      6583..6830
                     /gene="S. cerevisiae CYC1"
                     /note="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     misc_feature    6843..7287
                     /note="KmRPR1"
     misc_feature    6961..6971
                     /note="A-Box"
     misc_feature    7001..7009
                     /note="B-Box"
     misc_feature    7288..7295
                     /note="NotI cutsite"
     misc_feature    7319..7356
                     /note="trna"
     misc_feature    7326..7336
                     /note="A-Box"
     misc_feature    7357..7393
                     /note="intron"
     misc_feature    7394..7428
                     /note="trna"
     misc_feature    7408..7416
                     /note="B-Box"
     misc_feature    7430..7437
                     /note="PspXI cutsite"
     misc_feature    7438..7457
                     /note="XDH1 target"
     misc_feature    7458..7535
                     /note="Structural gRNA element"
     misc_feature    7536..7555
                     /note="SUP4"
     CDS             complement(7568..7623)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="fragment of beta-galactosidase"
                     /note="lacZ'"
                     /translation="MTMITPSSELTLTKGNKS"
     promoter        complement(7580..7598)
                     /note="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    7589..7594
                     /note="End confirmed gRNA seq T3"
                     /note="Sequence weird after this point"
     primer_bind     complement(7619..7635)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    7619..7635
                     /note="M13R"
     protein_bind    7643..7659
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7667..7697)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     rep_origin      complement(8021..8609)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8780..9640)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9641..9745)
                     /gene="bla"
                     /note="AmpR promoter"
     repeat_region   9777..11042
                     /note="Km CEN/ARS"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accacgcttt tcaattcaat tcatcatttt ttttttattc ttttttttga tttcggtttc
      241 tttgaaattt ttttgattcg gtaatctccg aacagaagga agaacgaagg aaggagcaca
      301 gacttagatt ggtatatata cgcatatgta gtgttgaaga aacatgaaat tgcccagtat
      361 tcttaaccca actgcacaga acaaaaacct gcaggaaacg aagataaatc atgtcgaaag
      421 ctacatataa ggaacgtgct gctactcatc ctagtcctgt tgctgccaag ctatttaata
      481 tcatgcacga aaagcaaaca aacttgtgtg cttcattgga tgttcgtacc accaaggaat
      541 tactggagtt agttgaagca ttaggtccca aaatttgttt actaaaaaca catgtggata
      601 tcttgactga tttttccatg gagggcacag ttaagccgct aaaggcatta tccgccaagt
      661 acaatttttt actcttcgaa gacagaaaat ttgctgacat tggtaataca gtcaaattgc
      721 agtactctgc gggtgtatac agaatagcag aatgggcaga cattacgaat gcacacggtg
      781 tggtgggccc aggtattgtt agcggtttga agcaggcggc agaagaagta acaaaggaac
      841 ctagaggcct tttgatgtta gcagaattgt catgcaaggg ctccctatct actggagaat
      901 atactaaggg tactgttgac attgcgaaga gcgacaaaga ttttgttatc ggctttattg
      961 ctcaaagaga catgggtgga agagatgaag gttacgattg gttgattatg acacccggtg
     1021 tgggtttaga tgacaaggga gacgcattgg gtcaacagta tagaaccgtg gatgatgtgg
     1081 tctctacagg atctgacatt attattgttg gaagaggact atttgcaaag ggaagggatg
     1141 ctaaggtaga gggtgaacgt tacagaaaag caggctggga agcatatttg agaagatgcg
     1201 gccagcaaaa ctaaaaaact gtattataag taaatgcatg tatactaaac tcacaaatta
     1261 gagcttcaat ttaattatat cagttattac cctgcggtgt gaaataccgc acagatgcgt
     1321 aaggagaaaa taccgcatca ggaaattgta aacgttaata ttttgttaaa attcgcgtta
     1381 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     1441 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     1501 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     1561 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
     1621 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     1681 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     1741 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg
     1801 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
     1861 ctattacgcc agctggcgaa ggggggatgt gctgcaaggc gattaagttg ggtaacgcca
     1921 gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta
     1981 tagggcgaat tggagctcca catagcttca aaatgtttct actccttttt tactcttcca
     2041 gattttctcg gactccgcgc atcgccgtac cacttcaaaa cacccaagca cagcatacta
     2101 aatttcccct ctttcttcct ctagggtgtc gttaattacc cgtactaaag gtttggaaaa
     2161 gaaaaaagag accgcctcgt ttctttttct tcgtcgaaaa aggcaataaa aatttttatc
     2221 acgtttcttt ttcttgaaaa tttttttttt gatttttttc tctttcgatg acctcccatt
     2281 gatatttaag ttaataaacg gtcttcaatt tctcaagttt cagtttcatt tttcttgttc
     2341 tattacaact ttttttactt cttgctcatt agaaagaaag catagcaatc taatctaagt
     2401 tttctagaac tagtggatcc cccgggaaaa atggacaaga agtactctat cggtttggac
     2461 atcggtacca actctgttgg ttgggctgtt atcaccgacg aatacaaggt tccatctaag
     2521 aagttcaagg ttttgggtaa caccgacaga cactctatta agaagaattt gatcggtgct
     2581 ttgttgttcg actctggtga gaccgctgaa gctaccagat tgaagagaac cgctcgtaga
     2641 agatacacca gaagaaagaa cagaatctgt tatttgcaag aaattttctc taacgagatg
     2701 gctaaggtcg acgattcttt ctttcacaga ttggaagaat ctttcttggt cgaagaggat
     2761 aagaaacacg aaagacaccc aatcttcggt aacattgttg acgaagttgc ttaccacgaa
     2821 aagtacccaa ccatctacca tttgagaaaa aagttggttg attccaccga caaggctgac
     2881 ttgagattga tctacttggc cttggcccac atgatcaagt tcagaggtca cttcttgatt
     2941 gaaggtgact tgaacccaga caattctgac gttgacaagt tgttcatcca attggttcaa
     3001 acctacaacc aattgttcga ggaaaatcca atcaacgctt ccggtgtcga cgccaaagcc
     3061 attttgtccg ctagattatc taaatctcgt agattagaaa acttgattgc ccaattgcca
     3121 ggtgagaaga agaatggttt gttcggtaac ttgatcgcct tgtccttggg tttaacccca
     3181 aatttcaagt ctaatttcga cttggctgag gatgctaagt tacaattgtc caaggacacc
     3241 tacgacgatg acttggataa cttattggct caaattggtg atcaatacgc cgacttattc
     3301 ttggctgcca agaatttgtc tgatgccatc ttgttgtccg atatcttgag agttaacacc
     3361 gaaatcacca aggctccatt gtccgcttct atgatcaaga gatacgatga acatcatcaa
     3421 gatttgacct tattgaaagc tttggttaga caacagttgc cagaaaatta caaggaaatt
     3481 ttctttgatc aatctaagaa cggttatgct ggttacatcg atggtggtgc gtctcaagag
     3541 gaattctaca agttcattaa gccaatcttg gagaagatgg acggtaccga agaattgttg
     3601 gttaagttga acagagaaga tttgttgaga aagcaaagaa ccttcgacaa cggttctatc
     3661 ccacaccaaa tccacttggg tgaattgcac gctatcttga gaagacaaga agatttctac
     3721 ccattcttga aggataacag agagaagatc gaaaagattt tgaccttcag aatcccatac
     3781 tacgttggtc cattggctag aggtaactct agattcgctt ggatgaccag aaagtctgaa
     3841 gaaaccatca ccccatggaa cttcgaagaa gtcgttgaca agggtgcttc tgctcaatct
     3901 ttcatcgaaa gaatgaccaa cttcgacaag aacttgccaa acgaaaaggt tttgccaaag
     3961 cactctttgt tgtacgaata cttcaccgtt tacaacgaat tgaccaaggt taagtacgtt
     4021 accgaaggta tgagaaagcc agctttcttg tctggtgaac aaaagaaggc tatcgttgac
     4081 ttgttgttca agactaacag aaaggttacc gtcaagcagt tgaaggaaga ttacttcaag
     4141 aagatcgaat gtttcgactc tgtcgaaatt tctggtgtcg aagacagatt caatgcttct
     4201 ttgggtactt accacgattt gttgaaaatc attaaggata aggatttctt agataacgag
     4261 gagaacgaag atattttgga ggatattgtc ttgactttga ctttgtttga ggatagagaa
     4321 atgatcgagg aaagattgaa gacttacgct catttgttcg atgataaggt catgaagcaa
     4381 ttgaagagaa gaagatacac tggttggggt agattgtcca gaaagttgat caacggtatt
     4441 agagataagc aatccggtaa gaccattttg gatttcttga agtctgacgg tttcgctaat
     4501 agaaacttta tgcaattgat tcacgacgat tctttgactt ttaaggaaga tattcaaaag
     4561 gctcaagtct ctggtcaggg tgattctttg cacgaacaca tcgctaactt ggctggttct
     4621 ccagctatca aaaaaggtat tttgcaaact gttaaggttg ttgacgaatt ggttaaggtt
     4681 atgggtagac acaagccaga gaatattgtc atcgagatgg ctagagaaaa tcaaaccact
     4741 caaaagggtc aaaagaactc cagagagaga atgaaaagaa ttgaggaagg tatcaaggag
     4801 ttgggttccc aaattttgaa ggaacaccca gtcgagaaca ctcaattgca aaatgaaaag
     4861 ttgtacttgt attacttgca aaacggtaga gatatgtacg ttgaccaaga attggacatt
     4921 aacagattgt ctgactacga tgttgatcac atcgttccac aatccttttt gaaggatgac
     4981 tctatcgata acaaggtttt gactagatcc gacaagaata gaggtaagtc tgataacgtt
     5041 ccatctgaag aagtcgtcaa gaagatgaag aactactgga gacagttgtt gaatgctaag
     5101 ttgattactc aaagaaagtt cgataacttg actaaggctg agagaggtgg tttgtctgag
     5161 ttggataagg ccggtttcat taagagacaa ttggtcgaaa ccagacaaat caccaagcac
     5221 gttgctcaaa tcttggactc tagaatgaac accaagtacg acgaaaacga caaattgatt
     5281 agagaggtta aggtcatcac cttgaagtcc aaattggttt ccgactttag aaaggatttc
     5341 caattctaca aggtcagaga aatcaataac taccaccatg ctcacgacgc ctacttgaac
     5401 gctgttgtcg gtactgcttt gatcaagaag tacccaaagt tggagtctga atttgtctac
     5461 ggtgattaca aagtttacga cgttagaaag atgattgcta agtctgagca agaaatcggt
     5521 aaggctactg ctaaatactt cttctactcc aacattatga atttcttcaa gaccgagatt
     5581 accttggcca acggtgaaat cagaaagaga ccattgattg aaactaacgg tgaaaccggt
     5641 gagattgtct gggacaaggg tagagatttc gctactgtta gaaaggtctt gtctatgcca
     5701 caagtcaaca ttgtcaagaa gaccgaagtc cagactggtg gtttctccaa ggaatctatt
     5761 ttgccaaaga gaaactctga taagttgatt gccagaaaga aagactggga tccaaagaag
     5821 tacggtggtt tcgactctcc aaccgttgct tactccgttt tggttgtcgc taaggttgaa
     5881 aagggtaagt ccaagaagtt gaagtccgtc aaggagttgt tgggtattac catcatggaa
     5941 agatcttcct tcgaaaagaa cccaattgat ttcttggagg ctaagggtta caaggaagtc
     6001 aagaaggact tgatcattaa gttgccaaag tactctttgt tcgaattgga gaacggtaga
     6061 aagagaatgt tggcttccgc tggtgaattg caaaagggta acgaattggc tttgccatct
     6121 aagtacgtta acttcttgta cttggcttcc cactacgaaa agttgaaggg ttctccagaa
     6181 gataacgaac aaaagcaatt gttcgttgaa caacataagc actacttgga cgaaatcatc
     6241 gaacaaatct ctgaattctc taagagagtt atcttggctg acgctaactt ggacaaggtt
     6301 ttgtctgctt acaacaagca cagagacaag ccaatcagag agcaagctga aaacatcatc
     6361 cacttgttca ctttgaccaa cttgggtgct ccagctgctt tcaagtactt cgacaccacc
     6421 atcgacagaa agagatacac ctctaccaag gaagttttgg acgctacctt gatccaccaa
     6481 tctatcaccg gtttgtacga aaccagaatc gacttgtctc aattgggtgg tgactctaga
     6541 gctgacccaa agaagaagag aaaggtttga tctcttctcg agtcatgtaa ttagttatgt
     6601 cacgcttaca ttcacgccct ccccccacat ccgctctaac cgaaaaggaa ggagttagac
     6661 aacctgaagt ctaggtccct atttattttt ttatagttat gttagtatta agaacgttat
     6721 ttatatttca aatttttctt ttttttctgt acagacgcgt gtacgcatgt aacattatac
     6781 tgaaaacctt gcttgagaag gttttgggac gctcgaaggc tttaatttgc cttcctaggg
     6841 cgtatactcc aacttggtcg aaagatgttt cagtgactgt gtcaacgact ttacctctgc
     6901 taagctaact tccaaggagc aaagctgcat aatgaaatgc tcagaaaagt tcttgaaaca
     6961 tagtgaacgt gttggacaac gtttccaaga gcaaaacgct gctttgaacc aaagcatggg
     7021 tcgttaagtt atacaaacag ttatgatata aatagttata gctttctctc tgtatatagc
     7081 caatatagct actaatagtt cacataaaca agtgctgcac gatgtgtaaa ggaacttatc
     7141 tataatagat ttcaaatatc aaccactact actcactata ctcagtgatg gccgatgtca
     7201 taagaatcgc taatataaat taataggcga atgcacaaga agcataccaa gaaaataaaa
     7261 gttgaaggaa aaagagagaa tttagatgcg gccgcaattt ctcttctacc acgaactcga
     7321 cactgtagtc tagtggttta ggatactccc cttccactta acaagtttct tcctaaaaag
     7381 aagtaacaag gcgagggagt gacacgggtt cgaatcccgt cagtgtcaac ctcgaggacg
     7441 atcgccaacc ttgaccattt tagagctaga aatagcaagt taaaataagg ctagtccgtt
     7501 atcaacttga aaaagtggca ccgagtcggt ggtgcttttt ttgtttttta tgtctctgcc
     7561 gcggtaccag cttttgttcc ctttagtgag ggttaattcc gagcttggcg taatcatggt
     7621 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac ataggagccg
     7681 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag gtaactcaca ttaattgcgt
     7741 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     7801 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     7861 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     7921 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     7981 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctcggccccc
     8041 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     8101 aaagatacca ggcgttcccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     8161 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct
     8221 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     8281 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     8341 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     8401 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     8461 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     8521 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     8581 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     8641 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     8701 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     8761 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     8821 gtctatttcg ttcatccata gttgcctgac tgcccgtcgt gtagataact acgatacggg
     8881 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     8941 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     9001 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     9061 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     9121 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     9181 ccatgttgtg aaaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     9241 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     9301 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     9361 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     9421 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     9481 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     9541 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     9601 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     9661 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     9721 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgagc
     9781 tcctttcatt tctgataaaa gtaagattac tccatttatc ttttcaccaa catattcata
     9841 gttgaaagtt atccttctaa gtacgtatac aatattaatt aaacgtaaaa acaaaactga
     9901 ctgtaaaaat gtgtaaaaaa aaaatatcaa attcatagca gtttcaagga atgaaaacta
     9961 ttatgatctg gtcacgtgta tataaattat taattttaaa cccatataat ttattatttt
    10021 tttattctaa agtttaaagt aattttagta gtattttata ttttgaataa atatacttta
    10081 aatttttatt tttatatttt attactttta aaaataatgt ttttatttaa aacaaaatta
    10141 taagttaaaa agttgttccg aaagtaaaat atattttata gtttttacaa aaataaatta
    10201 tttttaacgt atttttttta attatatttt tgtatgtgat tatatccaca ggtattatgc
    10261 tgaatttagc tgtttcagtt taccagtgtg atagtatgat tttttttgcc tctcaaaagc
    10321 tattttttta gaagcttcgt cttagaaata ggtggtgtat aaattgcggt tgacttttaa
    10381 ctatatatca ttttcgattt atttattaca tagagaggtg cttttaattt tttaattttt
    10441 attttcaata attttaaaag tgggtacttt taaattggaa caaagtgaaa aatatctgtt
    10501 atacgtgcaa ctgaatttta ctgaccttaa aggactatct caatcctggt tcagaaatcc
    10561 ttgaaatgat tgatatgttg gtggattttc tctgattttc aaacaagagg tattttattt
    10621 catatttatt atatttttta catttatttt atattttttt attgtttgga agggaaagcg
    10681 acaatcaaat tcaaaatata ttaattaaac tgtaatactt aataagagac aaataacagc
    10741 caagaatcaa atactgggtt tttaatcaaa agatctctct acatgcaccc aaattcatta
    10801 tttaaattta ctatactaca gacagaatat acgaacccag attaagtagt cagacgcttt
    10861 tccgctttat tgagtatata gccttacata ttttctgccc ataatttctg gatttaaaat
    10921 aaacaaaaat ggttactttg tagttatgaa aaaaggcttt tccaaaatgc gaaatacgtg
    10981 ttatttaagg ttaatcaaca aaacgcatat ccatatgggt agttggacaa aacttcaatc
    11041 gatgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
    11101 aggccctttc gtc
//
