LOCUS       Exported                6349 bp ds-DNA     circular SYN 25-JAN-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pXY027(Plac-FtsZ-GFP)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jun 14, 2017 from SnapGene 3.2.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6349
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        80..124
                     /note="T5 promoter"
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    100..116
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    132..148
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             167..178
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_feature    191..1339
                     /label=FtsZ
                     /note="FtsZ"
     misc_feature    1358..2068
                     /label=GFP
                     /note="GFP"
     terminator      2117..2211
                     /note="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     promoter        2767..2869
                     /note="cat promoter"
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             2870..3529
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /note="CmR"
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     rep_origin      4078..4666
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        4910..4987
                     /gene="lacI (mutant)"
                     /note="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             4988..6070
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /note="lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        6119..6149
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    6157..6173
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6181..6197
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6213..6229)
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
       61 tcgtcttcac ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat
      121 aatagattca attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt
      181 aactactagt atgtttgaac caatggaact taccaatgac gcggtgatta aagtcatcgg
      241 cgtcggcggc ggcggcggta atgctgttga acacatggtg cgcgagcgca ttgaaggtgt
      301 tgaattcttc gcggtaaata ccgatgcaca agcgctgcgt aaaacagcgg ttggacagac
      361 gattcaaatc ggtagcggta tcaccaaagg actgggcgct ggcgctaatc cagaagttgg
      421 ccgcaatgcg gctgatgagg atcgcgatgc attgcgtgcg gcgctggaag gtgcagacat
      481 ggtctttatt gctgcgggta tgggtggtgg taccggtaca ggtgcagcac cagtcgtcgc
      541 tgaagtggca aaagatttgg gtatcctgac cgttgctgtc gtcactaagc ctttcaactt
      601 tgaaggcaag aagcgtatgg cattcgcgga gcaggggatc actgaactgt ccaagcatgt
      661 ggactctctg atcactatcc cgaacgacaa actgctgaaa gttctgggcc gcggtatctc
      721 cctgctggat gcgtttggcg cagcgaacga tgtactgaaa ggcgctgtgc aaggtatcgc
      781 tgaactgatt actcgtccgg gtttgatgaa cgtggacttt gcagacgtac gcaccgtaat
      841 gtctgagatg ggctacgcaa tgatgggttc tggcgtggcg agcggtgaag accgtgcgga
      901 agaagctgct gaaatggcta tctcttctcc gctgctggaa gatatcgacc tgtctggcgc
      961 gcgcggcgtg ctggttaaca tcacggcggg cttcgatctg cgtctggatg agttcgaaac
     1021 ggtaggtaac accatccgtg catttgcttc cgacaacgcg actgtggtta tcggtacttc
     1081 tcttgacccg gatatgaatg acgagctgcg cgtaaccgtt gttgcgacag gtatcggcat
     1141 ggacaaacgt cctgaaatca ctctggtgac caataagcag gttcagcagc cagtgatgga
     1201 tcgctaccag cagcatggga tggctccgct gacccaggag cagaagccgg ttgctaaagt
     1261 cgtgaatgac aatgcgccgc aaactgcgaa agagccggat tatctggata tcccagcatt
     1321 cctgcgtaag caagctgatg gcctatgcgg ccgcagtaaa ggagaagaac ttttcactgg
     1381 agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag
     1441 tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac
     1501 tggaaaacta cctgttccat ggccaacact tgtcactact ctgacgtatg gtgttcaatg
     1561 cttttcccgt tatccggatc atatgaaacg gcatgacttt ttcaagagtg ccatgcccga
     1621 aggttatgta caggaacgca ctatatcttt caaagatgac gggaactaca agacgcgtgc
     1681 tgaagtcaag tttgaaggtg atacccttgt taatcgtatc gagttaaaag gtattgattt
     1741 taaagaagat ggaaacattc tcggacacaa actcgagtac aactataact cacacaatgt
     1801 atacatcacg gcagacaaac aaaagaatgg aatcaaagct aacttcaaaa ttcgccacaa
     1861 cattgaagat ggatccgttc aactagcaga ccattatcaa caaaatactc caattggcga
     1921 tggccctgtc cttttaccag acaaccatta cctgtcgaca caatctgccc ttttgaaaga
     1981 tcccaacgaa aagcgtgacc acatggtcct tcttgagttt gtaactgctg ctgggattac
     2041 acatggcatg gatgaactat acaaataagc ggccgctaag ggtcgacctg cagccaagct
     2101 taattagctg agcttggact cctgttgata gatccagtaa tgacctcaga actccatctg
     2161 gatttgttca gaacgctcgg ttgccgccgg gcgtttttta ttggtgagaa tccaagctag
     2221 gggaattcat cgtgactgac tgacgatctg cctcgcgcgt ttcggtgatg acggtgaaaa
     2281 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     2341 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac
     2401 ccagtcacgt agcgatagcg gagtgtataa ttcttgaaga cgaaagggcc tcgtgatacg
     2461 cctattttta taggttaatg tcatgataat aatggtttct taggatcctg atgtccggcg
     2521 gtgcttttgc cgttacgcac caccccgtca gtagctgaac aggagggaca gctgatagaa
     2581 acagaagcca ctggagcacc tcaaaaacac catcatacac taaatcagta agttggcagc
     2641 atcacccgac gcactttgcg ccgaataaat acctgtgacg gaagatcact tcgcagaata
     2701 aataaatcct ggtgtccctg ttgataccgg gaagccctgg gccaactttt ggcgaaaatg
     2761 agacgttgat cggcacgtaa gaggttccaa ctttcaccat aatgaaataa gatcactacc
     2821 gggcgtattt tttgagttat cgagattttc aggagctaag gaagctaaaa tggagaaaaa
     2881 aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc
     2941 atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt
     3001 tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc
     3061 ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg agctggtgat
     3121 atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc
     3181 gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt
     3241 ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt
     3301 cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga
     3361 caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct
     3421 gatgccgctg gcgattcagg ttcatcatgc cgtctgtgat ggcttccatg tcggcagaat
     3481 gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaat ttttttaagg
     3541 cagttattgg tgcccttaaa cgcctggtgc tacgcctgaa taagtgataa taagcggatg
     3601 aatggcagaa attcgaaagc aaattcgacc cggtcgtcgg ttcagggcag ggtcgttaaa
     3661 tagccgctta tgtctattgc tggtttaccg gtttattgac taccggaagc agtgtgaccg
     3721 tgtgcttctc aaatgcctga ggccagtttg ctcaggctct ccccgtggag gtaataattg
     3781 acgatatgat catttattct gcctcccaga gcctgataaa aacggttagg atcggagtca
     3841 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     3901 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     3961 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     4021 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     4081 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     4141 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     4201 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     4261 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     4321 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     4381 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     4441 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     4501 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     4561 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     4621 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     4681 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     4741 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     4801 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg
     4861 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc ataaattccg acaccatcga
     4921 atggtgcaaa acctttcgcg gtatggcatg atagcgcccg gaagagagtc aattcagggt
     4981 ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag tatgccggtg tctcttatca
     5041 gaccgtttcc cgcgtggtga accaggccag ccacgtttct gcgaaaacgc gggaaaaagt
     5101 ggaagcggcg atggcggagc tgaattacat tcccaaccgc gtggcacaac aactggcggg
     5161 caaacagtcg ttgctgattg gcgttgccac ctccagtctg gccctgcacg cgccgtcgca
     5221 aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg tggtgtcgat
     5281 ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg
     5341 cgtcagtggg ctgatcatta actatccgct ggatgaccag gatgccattg ctgtggaagc
     5401 tgcctgcact aatgttccgg cgttatttct tgatgtctct gaccagacac ccatcaacag
     5461 tattattttc tcccatgaag acggtacgcg actgggcgtg gagcatctgg tcgcattggg
     5521 tcaccagcaa atcgcgctgt tagcgggccc attaagttct gtctcggcgc gtctgcgtct
     5581 ggctggctgg cataaatatc tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg
     5641 cgactggagt gccatgtccg gttttcaaca aaccatgcaa atgctgaatg agggcatcgt
     5701 tcccactgcg atgctggttg ccaacgatca gatggcgctg ggcgcaatgc gcgccattac
     5761 cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg ataccgaaga
     5821 cagctcatgt tatatcccgc cgttaaccac catcaaacag gattttcgcc tgctggggca
     5881 aaccagcgtg gaccgcttgc tgcaactctc tcagggccag gcggtgaagg gcaatcagct
     5941 gttgcccgtc tcactggtga aaagaaaaac caccctggcg cccaatacgc aaaccgcctc
     6001 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     6061 cgggcagtga gcgcaacgca attaatgtaa gttagctcac tcattaggca ccccaggctt
     6121 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     6181 caggaaacag ctatgaccat gattacggat tcactggccg tcgttttaca acgtcgtgac
     6241 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     6301 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgc
//
