LOCUS       BBa_J23100_PROPS        3155 bp ds-DNA     linear       12-JUN-2017
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     100-PROPS Example Construct version 1 (still working out some kinks
            while comparing to sequencing)
COMMENT     This is an example construct using the backbone (BBa_J61002) +
            promoter (BBa_J23100) + RBS-PROPS-TERMINATOR  (all three components
            were taken from pBAD)
COMMENT     Question: Am I missing some of the nts that were rescued when the
            promoter was cloned into this backbone (cloned between XBA1/SPE1? I
            just pasted the promoter in but that may not actually be accurate
COMMENT     If this is the case, it would not really matter for expression but
            it could be confusing and problematic using traditional restriction
            digest/ligation cloning. I should compare some sequencing results
            to the backbone.
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     rep_origin      complement(1631..2313)
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    441..1211
                     /label=GPR D97N
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=#004080
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(2411..3070)
                     /label=AmpR
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    412..428
                     /label=RBS (T7)
                     /ApEinfo_fwdcolor=#800080
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1530..1549
                     /label=Reverse Sequencing Primer Binding Site (VR)
                     /ApEinfo_fwdcolor=#8000ff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1314..1471
                     /label=rrnB_terminator
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    411..411
                     /label=SpeI digest relic nt
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#004080
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1472..1472
                     /label=PstI digest relic nt
                     /ApEinfo_fwdcolor=#0080c0
                     /ApEinfo_revcolor=#ff0080
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    236..255
                     /label=Forward Sequencing Primer Site (VF2)
                     /ApEinfo_fwdcolor=#0080ff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    411..411
                     /label=Insert Goes between these two nt, include RBS and T
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#800040
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1472..1472
                     /label=Insert Goes between these two nt, include RBS and
                     T(1)
                     /ApEinfo_label=Insert Goes between these two nt, include
                     RBS and T
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#800040
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    369..374
                     /label=XBA1 site, 3' end of promoter insert site
                     /ApEinfo_fwdcolor=#8080c0
                     /ApEinfo_revcolor=#000080
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    376..410
                     /label=BBa_J23118 Strong Constituative
                     /ApEinfo_fwdcolor=#235afa
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
       61 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
      121 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
      181 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
      241 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
      301 aggcagaatt tcagataaaa aaaatcctta gctttcgcta aggatgattt ctggaattcg
      361 cggccgcatc tagagttgac ggctagctca gtcctaggta ttgtgctagc aTTAACTTTA
      421 AGAAGGAGAT ATACATACCC ATGGGTAAAT TATTACTGAT ATTAGGTAGT GTTATTGCAC
      481 TTCCTACATT TGCTGCAGGT GGTGGTGACC TTGATGCTAG TGATTACACT GGTGTTTCTT
      541 TTTGGTTAGT TACTGCTGCT TTATTAGCAT CTACTGTATT TTTCTTTGTT GAAAGAGATA
      601 GAGTTTCTGC AAAATGGAAA ACATCATTAA CTGTATCTGG TCTTGTTACT GGTATTGCTT
      661 TCTGGCATTA CATGTACATG AGAGGGGTAT GGATTGAAAC TGGTGATTCG CCAACTGTAT
      721 TTAGATACAT TAATTGGTTA CTAACAGTTC CTCTATTAAT ATGTGAATTC TACTTAATTC
      781 TTGCTGCTGC AACTAATGTT GCTGGATCAT TATTTAAGAA ATTACTAGTT GGTTCTCTTG
      841 TTATGCTTGT GTTTGGTTAC ATGGGTGAAG CAGGAATCAT GGCTGCATGG CCTGCATTCA
      901 TTATTGGGTG TTTAGCTTGG GTATACATGA TTTATGAATT ATGGGCTGGA GAAGGAAAAT
      961 CTGCATGTAA TACTGCAAGT CCTGCTGTGC AATCAGCTTA CAACACAATG ATGTATATTA
     1021 TCATCTTTGG TTGGGCGATT TATCCTGTAG GTTATTTCAC AGGTTACCTG ATGGGTGACG
     1081 GTGGATCAGC TCTTAACTTA AACCTTATCT ATAACCTTGC TGACTTTGTT AACAAGATTC
     1141 TATTTGGTTT AATTATATGG AATGTTGCTG TTAAAGAATC TTCTAATGCT CACCATCACC
     1201 ATCACCATTG AGTTTAAACG GTCTCCAGCT TGGCTGTTTT GGCGGATGAG AGAAGATTTT
     1261 CAGCCTGATA CAGATTAAAT CAGAACGCAG AAGCGGTCTG ATAAAACAGA ATTTGCCTGG
     1321 CGGCAGTAGC GCGGTGGTCC CACCTGACCC CATGCCGAAC TCAGAAGTGA AACGCCGTAG
     1381 CGCCGATGGT AGTGTGGGGT CTCCCCATGC GAGAGTAGGG AACTGCCAGG CATCAAATAA
     1441 AACGAAAGGC TCAGTCGAAA GACTGGGCCT Tggcttcctc gctcactgac tcgctgcgct
     1501 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     1561 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     1621 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     1681 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     1741 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     1801 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     1861 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     1921 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     1981 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     2041 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     2101 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     2161 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     2221 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     2281 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     2341 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     2401 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     2461 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     2521 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     2581 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     2641 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     2701 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     2761 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     2821 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     2881 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     2941 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     3001 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     3061 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     3121 tgagatccag ttcgatgtaa cccactcgtg caccc  
//
